Volume 12, Issue 2 ((Spring and Summer) 2023)
Plant Pathol. Sci. 2023, 12(2): 11-26 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Jahandideh M, Nematollahi S, Rakhshandehroo F. (2023). The reaction of four pumpkin varieties to cucumber mosaic virus by analyzing the expression of PAL and PR2 genes. Plant Pathol. Sci.. 12(2), 11-26. doi:10.52547/pps.12.2.11
URL: http://yujs.yu.ac.ir/pps/article-1-416-en.html
URL: http://yujs.yu.ac.ir/pps/article-1-416-en.html
Department of Plant Protection, College of Agricultural Sciences and Food Industries, Islamic Azad University Science and Research Branch, Tehran, Iran , nematollahi2001@yahoo.com
Abstract: (505 Views)
Jahandideh M, Nematollahi S, Rakhshandehroo F (2023) The reaction of four pumpkin varieties to cucumber mosaic virus by analyzing the expression of PAL and PR2 genes. Plant Pathology Science 12(2):11- 26.
Introduction: Cucumber mosaic virus (CMV) is one of the most important pathogenic cucurbit viruses. Identifying and growing resistant or tolerant varieties is the best method for disease control. The present study was conducted to investigate the response of four pumpkin varieties to CMV by assessing the expression of genes involved in resistance (PAL, PR2). Materials and Methods: Forty samples of pumpkin leaves with suspected disease were collected from the farms in northwestern Iran and analyzed using the TAS-ELISA test. To study the response of the four pumpkin varieties Asma, Pars, Prof and PS grown in this region, a CMV isolate was inoculated into the plants in a greenhouse experiment after its biological purification. TAS-ELISA and semi-quantitative (Sq) RT-PCR tests were used to examine the virus concentration in pumpkin varieties. The disease severity index was evaluated 30 days after inoculation. The expression level of PAL and PR2 genes was also checked by quantitative real-time PCR technique. Results: Of the 40 samples, 16 samples were infected with CMV. The study of virus titer revealed that the virus concentration and disease severity index were higher in Pars and Proof varieties than in PS and Asma varieties. The expression of PAL and PR2 genes was increased in all varieties compared to control but was higher in PS cultivar followed by Asma. Conclusion: PS and Asma varieties have higher CMV tolerance and their wider cultivation is recommended for disease control.
References
1. Abdelkhalek A(2019). Expression of tomato pathogenesis related genes in response to tobacco mosaic virus. Journal of Animal Plant Science 29:1596-1602.
2. Abtahi FS, Hatami M, Salehi-Arjmand H, Mahdieh M, Yazdani R(2021) Detection and phylogenetic analysis of new Iranian isolates of Cucumber mosaic virus on Achillea species. Journal of Crop Protection 10 (2): 309-317.
3. Arafati N, Farzadfar S, Pourrahim R(2013) Characterization of coat protein gene of Cucumber mosaic virus isolates in Iran. Iranian Journal of Biotechnology 11(2): 109-114. [DOI:10.5812/ijb.10715]
4. Arzani A, Ahoonmanesh A (2000) Study of resistance to Cucumber mosaic virus,Watermelon mosaic virus and Zucchini yellow mosaic virus in melon varieties. Iranian Agricultural Research 19:129-144.
5. Ashfaq M, Iqbal S, Mukhtar T, Shah H (2014) Screening for resistance to
6. Cucumber mosaic cucumovirus in Chilli Pepper. Journal of Animal Plant Science 24:791-795.
7. Buchanan BB (2000). Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists Rockville, Maryland, Pp: 610-628.
8. Chahook M, Seyyed Z, Masoomi H, Heydarnejhad J, Hosinipoor A (2021) Reaction of 40 genotypes of zucchini to cucumber mosaic virus. Knowledge of plant pathology 11 (1): 49-59. (In Persian).
9. Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475-483. [DOI:10.1099/0022-1317-34-3-475] [PMID]
10. Converse RH, Martin RR (1990). ELISA methods for plant viruses. In: R., Hampton, E., Ball and S. De Boer (Eds.). Serological Methods for Detection and Identification of Viral and Bacterial Plant Pathogens. A laboratory manual. APS Press. 389 pp
11. Cueto-Ginzo I, Serrano L, Sin E, Rodríguez R., Morales J, Lade S, Medina V, Achon M (2016). Exogenous salicylic acid treatment delays initial infection and counteracts alterations induced by Maize dwarf mosaic virus in the maize proteome. Physiological and Molecular Plant Pathology 96: 47-59. [DOI:10.1016/j.pmpp.2016.07.001]
12. ElMorsi, A., Abdelkhalek, A., Alshehaby, O. and Hafez, E.E. 2015. Pathogenesis-related genes as tools for discovering the response of onion defence system against Iris yellow spot virus infection. Botan. 93: 735-744. [DOI:10.1139/cjb-2015-0017]
13. Elsharkawy MM, Shimizu M, Takahashi H, Hyakumachi M (2012). The plant growth-promoting fungus Fusarium equiseti and the arbuscular mycorrhizal fungus Glomus mosseae induce systemic resistance against Cucumber mosaic virus in cucumber plants. Plant and Soil 361:397−409. [DOI:10.1007/s11104-012-1255-y]
14. Fitzgerald HA, Chern MS, Navarre R, Ronald PC (2004) Overexpression of (At) NPR1 in rice leads to a BTH-and environment-induced lesionmimic/cell death phenotype. Molecular Plant Microbe Interaction 17(2): 140-151. [DOI:10.1094/MPMI.2004.17.2.140] [PMID]
15. Gallitelli D (2000) The ecology of cucumber mosaic virus and sustainable agriculture. Virus Research 71: 9-21. [DOI:10.1016/S0168-1702(00)00184-2] [PMID]
16. Heydarnejhad A, Babayizad V, Rahimian H (2015). Studying the role of PAL and PR2 genes in rice plant resistance against bacteria Acidovorax avenae sub sp. Avenae . Biotechnology of Agriculture 7(4):67-81. (In Persian).
17. Jwa NS, Agrawal GK, Tamogami S, Yonekura M, Han O, Iwahashi H, Rakwal R (2006) Role of defense/stress-related marker genes, proteins and secondary metabolites in defining rice selfdefense mechanisms. Plant Physiology Biochemistry 44: 261- 273 [DOI:10.1016/j.plaphy.2006.06.010] [PMID]
18. Li N, Yu C, Yin Y, Gao S, Wang F, Jiao C,Yao M (2020) Pepper Crop Improvement Against Cucumber Mosaic Virus (CMV). Front Plant Science 11: 598-798. [DOI:10.3389/fpls.2020.598798] [PMID] []
19. Lin H, Rubio L, Smythe A, Jiminez M, Falk WB (2003) Genetic diversity and biological variation among California isolates of cucumber mosaic virus. Journal of General Virology 84:249-258. [DOI:10.1099/vir.0.18673-0] [PMID]
20. Marone M, Mozzetti S, Ritis D, Pierelli L, Scambia G (2001). Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biological Procedures 3:19-25. [DOI:10.1251/bpo20] [PMID] []
21. Martín-Hernández AM, Picó B, 2021. Natural resistances to viruses in cucurbits. Agronomy 11(23): 1-29. [DOI:10.3390/agronomy11010023]
22. Mirhosseini-chahooki SZ, Massumi H, Heydanejada J, Hosseinipour A, Abdoshahi R, Maddahian M, 2022. The reaction of forty cucurbits genotypes against Cucumber mosaic virus. Plant Pathology Science 11(1): 48-59. [DOI:10.52547/pps.11.1.48]
23. Nematollahi S, Sokhandan BN, Rakhshandehroo F, Zamanizadeh HR (2012) Phylogenetic analysis of new isolates of Cucumber mosaic virus from Iran on the basis of different genomic regions. The Plant Pathology Journal 28(4):381-389. [DOI:10.5423/PPJ.OA.06.2012.0077]
24. Nematollahi S, Sokhandan BN, Rakhshandehroo F, Zamanizadeh HR (2016) Investigating the biological and molecular characteristics of several isolates of Cucumber mosaic virus from northwestern Iran. Journal of Applied Research in Plant Protection 6 (3): 97-108. (In Persian).
25. Nisha S, Revathi K, Chandrasekaran R, Kirubakaran SA, Sathish-Narayanan S, Stout M J, Senthil-Nathan S (2012). Effect of plant compounds on induced activities of defenserelated enzymes and pathogenesis related protein in bacterial blight disease susceptible rice plant. Physiological and Molecular Plant Pathology 80: 1-9. [DOI:10.1016/j.pmpp.2012.07.001]
26. Nourbakhsh shourabi F, Shamsbakhsh M (2019). Cucumber mosaic virus and cauliflower mosaic virus contamination on rapeseed resistance to turnip mosaic virus. Knowledge of herbal medicine. Iran, 50 (1), 119-127. (In Persian)
27. Oide S, Bejai S, Staal J, Guan N, Kaliff M, Dixelius CA, (2013) novel role of PR 2 in abscisic acid (ABA) mediated, pathogen-induced callose deposition in Arabidopsis thaliana. New Phytologist 200: 1187-1199. [DOI:10.1111/nph.12436] [PMID]
28. Otulak-Kozieł K, Kozieł E, Lockhart B (2018) Plant cell wall dynamics in compatible and incompatible potato response to infection caused by potato virus Y (PVYNTN). International Journal of Molecular Science 19: 862-868. [DOI:10.3390/ijms19030862] [PMID] []
29. Palukaitis P, Garcia-Arenal F( 2003) Cucumoviruses. Advance Virus Research 62: 241-23. [DOI:10.1016/S0065-3527(03)62005-1] [PMID]
30. Peltonen S, Karjalainen R (1995). Phenylalanine ammonia-lyase activity in barley after infection withBipolaris sorokinianaor treatment with its puri®ed xylanase. Journal of Phytopathology 143: 239-245. [DOI:10.1111/j.1439-0434.1995.tb00606.x]
31. Peyman M,Ghannadha MR, Majidi E, Zarbakhsh A, Darvish F, Hasanabadi H (2004) Evaluation and introduction of virus resistant genotypes in potato. Iranatonal Journal of Agricultural Science 35(4): 809-815. (In Persian).
32. Phan MS, Seo J, Choi HS, Lee SH, Kim KH (2014) Pseudore combination between two distinct strains of Cucumber mosaic virus results in enhancement of symptom sseverity. The Plant Pathology Journal 30:316-322. [DOI:10.5423/PPJ.NT.04.2014.0031] [PMID] []
33. Pourrahim R., Farzadfar Sh,.Golnaraghi A R (2002) Basics of plant virology. Saman Pishegar. Press, 474 p. (In Persian).
34. Rakhshandehroo F, Takeshita M, Squires J, Palukaitis P (2009) The Influence of RNA-Dependent RNA Polymerase 1 on Potato virus Y Infection and on Other Antiviral Response Genes. Molecular Plant-Microbe Interaction 22: 1312-1318. [DOI:10.1094/MPMI-22-10-1312] [PMID]
35. Rizos, H., Gunn, L. V., Pares, R. D., and Gillings, R. M. 1992. Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. Journal of General Virology, 73:2099-2103. [DOI:10.1099/0022-1317-73-8-2099] [PMID]
36. Samiei A, Masoumi H, Shabaniyan M, Hosinpoor A, Heidarneghad J (2007). Evaluation of the response of different varieties of zucchini to 6 important viruses in field and greenhouse conditions. The Journal of Agriculture and Natural Resources Sciences 15:23-30. (In Persian).
37. Sindelarova M and Sindelar L (2005). Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV. Plant Prot. Sci. 41: 52-57. [DOI:10.17221/2747-PPS]
38. Sokhandan BN, Nematollahi S, Torabi E (2008) Cucumber mosaic virus subgroup IA frequently occurs in the northwest Iran. Acta Virologica 52(4):237-42.
39. Stotz HU, Thomson JG, Wang Y( 2009) Plant defensins Defense, development and application. Plant Signaling & Behavior 4: 1010-1012. [DOI:10.4161/psb.4.11.9755] [PMID] []
40. Vidhyasekaran P (2002) Bacterial disease resistance in plants: molecular biology and biotechnological applications. Routledge. 322p.
41. Xu H, Park NI, Li X, Kim YK, Lee SY, Park SU (2010) Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis. Biotechmology 101(24): 9715-9722. [DOI:10.1016/j.biortech.2010.07.083] [PMID]
42. Yang Y, Yan S, Wang H, Chen Z (1992) Screening and application of differential host of CMV strain on pepper. Virologica Sinica 7: 317-327.
43. Yuan J, Reed A, Chen F, Stewart C (2006). Statistical analysis of real time PCR data. BMC Bioinformatics 7: 85-90.
https://doi.org/10.1016/j.csda.2004.10.008 [DOI:10.1016/j.csda.2005.11.017]
44. Zeng R, Liao Q, Feng J, Li D, Chen J (2007). Synergy between Cucumber Mosaic Virus and Zucchini Yellow Mosaic Virus on Cucurbitaceae hosts tested by Real-time Reverse Transcription-Polymerase chain reaction. Acta Biochimica et Biophysica Sinica 39(6): 431-437. [DOI:10.1111/j.1745-7270.2007.00292.x] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
