جلد 12، شماره 2 - ( (بهار و تابستان) 1402 )
جلد 12 شماره 2 صفحات 26-11 |
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Jahandideh M, Nematollahi S, Rakhshandehroo F. (2023). The reaction of four pumpkin varieties to cucumber mosaic virus by analyzing the expression of PAL and PR2 genes. Plant Pathol. Sci.. 12(2), 11-26. doi:10.52547/pps.12.2.11
URL: http://yujs.yu.ac.ir/pps/article-1-416-fa.html
URL: http://yujs.yu.ac.ir/pps/article-1-416-fa.html
جهان دیده مهسا، نعمت اللهی سویل، رخشنده رو فرشاد. واکنش چهاررقم کدو به ویروس موزاییک خیار با آنالیز بیان ژنهای PAL و PR2 دانش بیماری شناسی گیاهی 1402; 12 (2) :26-11 10.52547/pps.12.2.11
گروه گیاه پزشکی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران ، nematollahi2001@yahoo.com
چکیده: (506 مشاهده)
جهاندیده م، نعمت اللهی س، رخشنده رو ف (1402) واکنش چهاررقم کدو به ویروس موزاییک خیار با آنالیز بیان ژنهای PAL و . PR2 دانش بیماریشناسی گیاهی 12(2): 26-11.
مقدمه: ویروس موزائیک خیار Cucumber mosaic virus, CMV)) یکی از مهمترین ویروسهای بیمارگر کدو است. شناسایی و کشت رقمهای مقاوم یا متحمل بهترین روش مدیریت بیماری است. پژوهش حاضر به منظور بررسی واکنش چهار رقم کدو نسبت به CMV با ارزیابی بیان ژنهای دخیل در مقاومت (PAL, PR2) انجام پذیرفت. مواد و روشها: چهل نمونه برگ کدوی مشکوک به بیماری از مزرعه های شمال غربی ایران جمع آوری و توسط آزمون TAS-ELISA بررسی شدند. یک جدایه CMV پس از خالصسازی بیولوژیکی برای بررسی واکنش چهار رقم کدوی تحت کشت در این منطقه به اسامی : اسما، پارس، پروف و PS در آزمایشی گلخانه ای به گیاهان مایه زنی شد. با آزمونهای TAS-ELISA و semi quantitative(Sq)-RT-PCR غلظت ویروس در رقمهای کدو بررسی و شاخص شدت بیماری تا ۳۰ روز پس از مایه زنی ارزیابی شد. میزان بیان ژن های PAL و PR2 نیز با تکنیک Real time PCR Quantitative بررسی شد. یافته ها: شانزده نمونه از مجموع 40 نمونه آلوده به CMV بودند. بررسی تیتر ویروس نشان داد که غلظت ویروس و شاخص شدت بیماری در دو رقم پارس و پروف نسبت به رقمهای PS و اسما بالاتر بود. بیان ژنهای PAL و PR2 در همه رقمها نسبت به شاهد افزایش داشت ولی در رقم PS و پس از آن اسما بیشتر بود. نتیجه گیری: رقمهای PS و اسما از تحمل بالاتری نسبت بهCMV برخوردار هستند و کشت وسیعتر آنها برای مدیریت مفید است.
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