Volume 13, Issue 2 ((Spring and Summer) 2024)
Plant Pathol. Sci. 2024, 13(2): 25-38 |
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Mohammadi S, Roosefid Z. (2024). Effect of Salvia mirzayanii and Mentha piperita essential oils on toxigenicity and aflatoxin gene expression in Aspergillus flavus. Plant Pathol. Sci.. 13(2), 25-38. doi:10.61186/pps.13.2.25
URL: http://yujs.yu.ac.ir/pps/article-1-453-en.html
URL: http://yujs.yu.ac.ir/pps/article-1-453-en.html
Department of Plant Pathology, Faculty of Agricultural Sciences and New Technologies, Islamic Azad University Shiraz Branch, Shiraz, Iran , Mohammadi.pp@gmail.com
Abstract: (531 Views)
Aflatoxins are toxic metabolites produced by certain Aspergillus species. They cause disease and contaminate food, so reducing and controlling these toxins is extremely important. Due to their antifungal properties, essential oils and extracts from medicinal plants are increasingly used. As part of this study, the medicinal plants Salvia mirzayanii and Mentha piperita were harvested from their original habitat in Hormozgan Province and essential oils were extracted. Subsequently, the toxin-producing fungus A. flavus was isolated and purified from maize kernels, and the effect of the essential oil of both plants on the toxigenicity of A. flavus and the expression of the aflatoxin-encoding gene was investigated using real-time PCR. The antifungal properties were assessed using the disk diffusion method and a mixed evaluation, and then the effective concentration of the essential oil in both plants was determined. The essential oils of both plants effectively inhibited the growth of A. flavus at concentrations of 1000 and 2000 ppm. However, peppermint essential oil significantly reduced toxin production and had a stronger effect on the expression of the gene encoding aflatoxin at a concentration of 2000 ppm. Considering the effectiveness of peppermint essential oil at a concentration of 2000 ppm on the expression of the gene encoding aflatoxin, this essential oil can be introduced as a biological and safe method for the biological control of aflatoxin.
References
1. Aasa, A., Fru, F., Adelusi, O., Oyeyinka, S., & Njobeh, P. (2023). A review of toxigenic fungi and mycotoxins in feeds and food commodities in West Africa. World Mycotoxin Journal, 16(1), 33-47. [DOI:10.3920/WMJ2021.2766]
2. Abbas, H. K., Cartwright, R. D., Xie, W., & Shier, W. T. (2006)Aflatoxin and fumonisin contamination of corn (maize, Zea mays) hybrids in Arkansas. Crop protection, 25(1), 1-9. [DOI:10.1016/j.cropro.2005.02.009]
3. Aeineh, M., Najafian, S., & Hosseinifarahi, M. (2023). Investigating the Phenological Stages of Essential Oil Yield and Essential Oil Compounds and Phytochemical Properties of Artemisia absinthium L.(Wormwood). Russian Journal of Plant Physiology, 70(6), 149. [DOI:10.1134/S1021443723601891]
4. Ali, N. (2019). Aflatoxins in rice: Worldwide occurrence and public health perspectives. Toxicology Reports, 6, 1188-1197. [DOI:10.1016/j.toxrep.2019.11.007] [PMID] []
5. Asgharnezhad, F., Mahdian, S., Amiri Besheli, B., & Jafar Nazari, S. (2014). Investigation of the production of B1, B2, G1 & G2 aflatoxins in some species of Aspergillus with high performance liquid chromatography (HPLC) methods. Research in Plant Pathology, 2(2), 55-70. https://rpp.marvdasht.iau.ir/article_561_16d33393254dd38b5adfa53b2d2fee7c.pdf
6. Brahmi, F., Khodir, M., Mohamed, C., & Pierre, D. (2017). Chemical composition and biological activities of Mentha species. Aromatic and medicinal plants-Back to nature, 10, 47-79.
7. Caceres, I., Khoury, A. A., Khoury, R. E., Lorber, S., Oswald, I. P., Khoury, A. E., . . . Bailly, J. D. (2020). Aflatoxin Biosynthesis and Genetic Regulation: A Review. Toxins (Basel), 12(3). [DOI:10.3390/toxins12030150] [PMID] []
8. Cai, J., Yan, R., Shi, J., Chen, J., Long, M., Wu, W., & Kuca, K. (2022). Antifungal and mycotoxin detoxification ability of essential oils: A review. Phytotherapy Research, 36(1), 62-72. [DOI:10.1002/ptr.7281] [PMID]
9. Ebrahimi, M., Gharehbagh, H. J., Dabaghian, F., Mojtabavi, S., Khademian, S., Saeedi, M., Khanavi, M. (2024). Salvia mirzayanii and Salvia hypoleuca Essential Oils: Chemical Composition, Alpha-Glucosidase Inhibitory, and Synergistic Effects of Selected Components. Jundishapur Journal of Natural Pharmaceutical Products(In Press). [DOI:10.5812/jjnpp-143185]
10. Fakhri, Y., Ghorbani, R., Taghavi, M., Keramati, H., Amanidaz, N., Moradi, B., Khaneghah, A. M. (2019). Concentration and prevalence of aflatoxin M1 in human breast milk in Iran: Systematic review, meta-analysis, and carcinogenic risk assessment: A review. Journal of Food Protection, 82(5), 785-795. [DOI:10.4315/0362-028X.JFP-18-367] [PMID]
11. Gallo, A., Solfrizzo, M., Epifani, F., Panzarini, G., & Perrone, G. (2016). Effect of temperature and water activity on gene expression and aflatoxin biosynthesis in Aspergillus flavus on almond medium. International Journal of Food Microbiology, 217, 162-169. [DOI:10.1016/j.ijfoodmicro.2015.10.026] [PMID]
12. Gorran, A., Salehnia, B., Farzaneh, H. R., Farzaneh, M., & Shivazad, M. (2015). Effect of essential oils and extracts of Satureja macrosiphon and Satureja khozistanica on mycelial growth and aflatoxin B1 production in Aspergillus flavus. Journal of Veterinary Research, 70(2), 139-145.
13. Lahooji, A., Mirabolfathy, M., & Karami-Osboo, R. (2010). Effect Of Zataria Multiflora and Satureja Hortensis Essential Oils, Thymol and Carvacrol on growth of Fusarium Gramineum Isolates and Deoxynivalenol production. Iranian Journal of Plant Pathology, 46(1), 37-50.
14. Lee, Y., Park, S. J., Kim, K., Kim, T.-O., & Lee, S.-E. (2023). Antifungal and antiaflatoxigenic activities of massoia essential oil and C10 massoia lactone against aflatoxin-producing Aspergillus flavus. Toxins (Basel), 15(9), 571. [DOI:10.3390/toxins15090571] [PMID] []
15. Liu, Y., & Wu, F. (2010). Global burden of aflatoxin-induced hepatocellular carcinoma: a risk assessment. Environ Health Perspect, 118(6), 818-824. [DOI:10.1289/ehp.0901388] [PMID] []
16. Mahendran, G., & Rahman, L. U. (2020). Ethnomedicinal, phytochemical and pharmacological updates on Peppermint (Mentha × piperita L.)-A review. Phytother. Res., 34(9), 2088-2139. [DOI:10.1002/ptr.6664] [PMID]
17. Mohammadi, M., Hashemi, S. J., Rezaie, S., & Bayat, M. (2018). Assessment of antifungal activity of Rosemary oil extract and its effect on AFL1 gene expression in Aspergillus flavus by Real-Time PCR. Journal of Microbial World, 11(1), 88-100.
18. Moon, Y.-S., Lee, H.-S., & Lee, S.-E. (2018). Inhibitory effects of three monoterpenes from ginger essential oil on growth and aflatoxin production of Aspergillus flavus and their gene regulation in aflatoxin biosynthesis. Applied Biological Chemistry, 61, 243-250. [DOI:10.1007/s13765-018-0352-x]
19. Nazir, A., Kalim, I., Sajjad, M., Usman, M., & Iqbal, M. (2019). Prevalence of aflatoxin contamination in pulses and spices in different regions of Punjab. Chem. Int., 5(4), 274.
20. Oyebamiji, Y. O., Shamsudin, N. A., Adebayo, I. A., Umar, O. B., Mohd Zaini, N. A., & Ismail, M. N. (2024). Aflatoxins in Cereals: Prevalence, Health Implications and Management Strategies: A Review. Egyptian Journal of Botany.
21. Pandey, D. K., Trpathi, N. N., Tripathi, R. D., & Dixit, S. N. (1982). Fungitoxic and phytotoxic properties of the essential oil of H. suaveolens. Zeit. Pflazenkraneaten and Pflanznschutz, 89, 344-349.
22. Raja, R. R. (2012). Medicinally potential plants of Labiatae (Lamiaceae) family: an overview. [DOI:10.3923/rjmp.2012.203.213]
23. Rouzbahan, S., Moein, S., & Homaei, A. (2016). Total antioxidant capacities and reduction of ferric ions by extracts of three medicinal plants and their fractions. Hormozgan Medical Journal, 20(4), 201-209.
24. Sahib, N. G., Anwar, F., Gilani, A. H., Hamid, A. A., Saari, N., & Alkharfy, K. M. (2013). Coriander (Coriandrum sativum L.): A potential source of high‐value components for functional foods and nutraceuticals‐A review. Phytotherapy Research, 27(10), 1439-1456. [DOI:10.1002/ptr.4897] [PMID]
25. Selma, M. V., Martínez-Culebras, P. V., & Aznar, R. (2008). Real-time PCR based procedures for detection and quantification of Aspergillus carbonarius in wine grapes. International Journal of Food Microbiology, 122(1-2), 126-134. [DOI:10.1016/j.ijfoodmicro.2007.11.049] [PMID]
26. Singh, R., Shushni, M. A., & Belkheir, A. (2015). Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry, 8(3), 322-328. [DOI:10.1016/j.arabjc.2011.01.019]
27. Tavaklipour, H., Zabar Jani, L., & Javanmard Dari, M. (2018). Comparison of the effect of sage extract and cumin in preventing toxin production of Aspergillus flavus fungus in pistachio nuts. Journal of Innovation in Food Science and Technology, 10, 11-24.
28. Temba, M. C., Njobeh, P., & Kayitesi, E. (2017). Storage stability of maize-groundnut composite flours and an assessment of aflatoxin B1 and ochratoxin A contamination in flours and porridges. Food Control, 71, 178-186. [DOI:10.1016/j.foodcont.2016.06.033]
29. Thathana, M. G., Murage, H., Abia, A. L. K., & Pillay, M. (2017). Morphological characterization and determination of aflatoxin-production potentials of Aspergillus flavus isolated from maize and soil in Kenya. Agriculture, 7(10), 80. [DOI:10.3390/agriculture7100080]
30. Thompson, D. (1989). Fungitoxic activity of essential oil components on food storage fungi. Mycologia, 81(1), 151-153. [DOI:10.1080/00275514.1989.12025637]
31. Valifard, M., Mohsenzadeh, S., Kholdebarin, B., & Rowshan, V. (2014). Effects of salt stress on volatile compounds, total phenolic content and antioxidant activities of Salvia mirzayanii. South African Journal of Botany, 93, 92-97. [DOI:10.1016/j.sajb.2014.04.002]
32. Weaver, M. A., Callicott, K. A., Mehl, H. L., Opoku, J., Park, L. C., Fields, K. S., & Mandel, J. R. (2022). Characterization of the Aspergillus flavus Population from Highly Aflatoxin-Contaminated Corn in the United States. Toxins (Basel), 14(11). [DOI:10.3390/toxins14110755] [PMID] []
33. Xiang, F., Zhao, Q., Zhao, K., Pei, H., & Tao, F. (2020). The efficacy of composite essential oils against aflatoxigenic fungus Aspergillus flavus in maize. Toxins (Basel), 12(9), 562. [DOI:10.3390/toxins12090562] [PMID] []
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