Volume 11, Issue 2 ((Spring and Summer) 2022)
Plant Pathol. Sci. 2022, 11(2): 61-72 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Moradi F, Mazaheri-laghab H, Kashi L, Mosavi S. (2022). Impact of raw and pure saponins of six alfalfa ecotypes on Ditylenchus dipsaci egg hatching. Plant Pathol. Sci.. 11(2), 61-72. doi:10.52547/pps.11.2.61
URL: http://yujs.yu.ac.ir/pps/article-1-375-en.html
URL: http://yujs.yu.ac.ir/pps/article-1-375-en.html
Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran , l.kashi@basu.ac.ir
Abstract: (1502 Views)
Moradi F, Mazaheri-laghab H, Kashi L, Moosavi SS (2022) Impact of raw and pure saponins of six alfalfa ecotypes on Ditylenchus dipsaci egg hatching. Plant Pathology Science 11(2):61-72. Doi: 10.2982/PPS.11.2.61
Introduction: The stem and bulb nematode, Ditylenchus dipsaci, is an important and damaging pathogen in a number of agricultural and ornamental plants, including alfalfa. The aim of this study was to investigate the effect of raw and pure saponins of six alfalfa ecotypes on the hatching of this nematode's eggs in order to find a biological method for its management. Materials and Methods: The effect of raw and pure saponins of six alfalfa ecotypes on the hatching of stem nematode eggs was investigated in a completely randomized factorial design with two factors of alfalfa ecotypes (six ecotypes) and their saponins (raw and pure) in vitro. Results: Analysis of variance showed that the interaction effect between two factors, ecotype and saponin, is statistically significant. At concentrations of 50 and 90 microliters of crude saponin from different ecotypes, 30-42% and 33-59% of the nematode eggs did not hatch, respectively. The Nishaburi ecotypes caused the most and the Shiraz and Khrisari polycross caused the least number of egg hatching. Concentrations of 10 and 50 microliters of pure saponin resulted in between 56 and 69% and 61 and 79% of total nematode eggs failing to hatch, respectively. The local ecotypes Miandoab and Nishaburi had the highest and Shiraz Polycross the lowest number of egg hatches. Conclusion: Pure saponins of alfalfa ecotypes have a greater effect on nematode egg hatching than raw saponins. Saponins of Shiraz Polycross alfalfa ecotype have a better effect than other ecotypes.
References
1. Akula R, Ravishankar GA (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior 6:1720-1731. [DOI:10.4161/psb.6.11.17613] [PMID] [PMCID]
2. Benelli G (2018) Plant-borne compounds and nanoparticles: Challenges for medicine, parasitology and entomology. Environmental and Science and Pollution Research. 25:10149-10150. [DOI:10.1007/s11356-017-9960-y] [PMID]
3. Cook R, Starr JL (2006) Resistant cultivars. In Plant Nematology, pp. 370-389. In: RN Perry and M Moens (eds.). Plant Nematology. Wallingford, CABI International. [DOI:10.1079/9781845930561.0370] [PMID]
4. D-addabbo T, Carbonara T, Leonetti P, Tava A, Avato P (2011) Control of plant parasitic nematodes with active saponins and biomass from Medicago sativa. Photochemistry Reviews 10:503-519. [DOI:10.1007/s11101-010-9180-2]
5. Fasihi M, Tanha Maafi Z, Kargar Bideh A, Eskandari A (2010) Host ranges variability, multiplication and seed-borne ability of some population of stem and bulb nematode (Ditylenchus dipsaci) in IRAN. Iranian Journal Plant Pathology 46:179-187. (In Persian with English Summary).
6. Faulkner LR, Bower DB, Evans DW, Elgin JH (1974) Mass culturing of Ditylenchus dipsaci to yield large quantities of inoculum. Journal of Nematology 6:126-129.
7. Francis G, Kerem Z, Makkar HPS, Becker K (2002) The biological action of saponins in animal systems: A Review. British Journal of Nutrition 88:587-605. [DOI:10.1079/BJN2002725] [PMID]
8. Golawska S, Leszczynski B, Oleszek W (2006) Effect of low and high-saponin lines of alfalfa on pea aphid. Journal of Insect Physiology 52:737-743. [DOI:10.1016/j.jinsphys.2006.04.001] [PMID]
9. Golawska S, Lukasik I, Wojcicka A, Sytykiewicz H (2012) Relationship between saponin content in alfalfa and aphid development. Acta Biological Cracoviensia Series Botanica 54:39-46. [DOI:10.2478/v10182-012-0022-y]
10. Hajihassani A, Tenuta M. Gulden RH (2016) Host preference and seed borne transmission of Ditylenchus wiescheri and D. dipsaci on select pulse and non-pulse crops grown in the Canadian prairies. Plant Disease 100:1087-1092. [DOI:10.1094/PDIS-11-15-1260-RE] [PMID]
11. IPPC (2016) Diagnostic protocols for regulated pests, Ditylenchus dipsaci and Ditylenchus destructor, Diagnostic protocols for regulated pests. International Plant Production Convention 1-34.
12. Kuhnhold V, Kiewnick S, Sikora RA (2006) Development of an in vivo bioassay to identify sugar beet resistance to the stem nematode Ditylenchus dipsaci. Nematology 8:641-645. [DOI:10.1163/156854106778877875]
13. Lamberti F, Sasnelli N, D-addabbo T, D-aloisio V, DE-cosmus P (2001) Chemical treatments and soil solarization for the control of the stem nematode (Ditylenchus dipsaci) on onions. Nematologia Mediterranea 29:149-152.
14. Madani M, Tenuta M, Chizhov VN, Subbotin SA (2015) Diagnostics of stem and bulb nematodes, Ditylenchus weischeri and D. dipsaci (Nematoda: Anguinidae), using PCR with species-specific primers. Canadian Journal of Plant Pathology 37:212-220. [DOI:10.1080/07060661.2015.1035754]
15. Mazahery-laghab H, Yazdi-samadi B (2004) Study of the resistance of alfalfa cultivars (Medicago sativa) to alfalfa weevil (Hypera postica Gyll.) attack under water stress conditions. Pajouhesh and Sazandegi 17:8-15. (In Persian with English Summary).
16. Navarro P, Giner RM, Recio M C, Manez S, Nicolas MC, Rios JL (2001) In vivo anti-inflammatory activity of saponins from Bupleurum rotundifolium. Life Science 68:1199-1206. [DOI:10.1016/S0024-3205(00)01019-5] [PMID]
17. Oleszek W, Stochmal A (2002) Triterpene saponins and flavonoids in the seeds of Trifolium species. Phytochemistry 61:165-170. [DOI:10.1016/S0031-9422(02)00230-3] [PMID]
18. Oleszek W, Jurzsta M, Ploszyvski M, Cplquhoun IA, Price KR, Fenwick GR (1992) Zahnic acid tridesmoside and other dominant saponins from alfalfa (Medicago sativa L.) aerial parts. Journal of Agricultural and Food Chemistry 40:191-196. [DOI:10.1021/jf00014a005]
19. Radovic J, Sokolovic D, Markovic J (2009) Alfalfa-most important perennial forage legume in animal husbandry. Biotechnology in Animal Husbandry 25:465-475. [DOI:10.2298/BAH0906465R]
20. Raju K, Jagadeewary MK, Satyanarayan K, Veeranna KC, Rajeshwari YB, Nagaraj CS, Shilpa SJ (2018) Intensive cultivation of Medicago sativa for sustainable milk production an action oriented approach. International Journal of Livestock Research. 8:101-108. [DOI:10.5455/ijlr.20171017055204]
21. Rosas-Hernandez L, Ramirez-Suarez A, Alcasio-Rangel S, Lopez-Buenfil JA, Mediva-Gomez E (2017) Detection, identification and phylogenetic inference of the stem nematode Ditylenchus dipsaci (Kuhn) filipjev (Nematoda: Anguinidae) affecting alfalfa Medicago sativa L. in Jalisco, Mexico. Mexican Journal of Phytopathology. 35:377-396. [DOI:10.18781/R.MEX.FIT.1703-8]
22. Saurabh P, Manila B, Tripathi N, Bansal YK (2015) Secondary metabolites of plants and their role: Overview. Current Trends in Biotechnology and Pharmacy 9:293-304.
23. Shany S, Gestener B, Brik Y, Bondi A, Kirson I (1972) Isolation of hederagenin and its saponin from alfalfa (Medicago Sativa). Israel Journal of Chemistry 10:881-884. [DOI:10.1002/ijch.197200088]
24. Taiz L, Zeiger E, Moller IM, Murphy A (2015) Plant physiology and development. 6th Edition, Sinauer Associates, Sunderland, CT.
25. Tava A, Avato P (2006) Chemical and biological activity of triterpene saponins from Medicago species. Natural Product Communication 1:1159-1180. [DOI:10.1177/1934578X0600101217]
26. Ustundag O, Mazza G (2007) Saponins: Properties, applications and processing. Critical Reviews in Food Science and Nutrition 47:231-258. [DOI:10.1080/10408390600698197] [PMID]
27. Vincken JP, Heng L, Degrot A, Gruppen H (2007) Saponins, classification and occurrence in the plant kingdom. Phytochemistry 68:275-297. [DOI:10.1016/j.phytochem.2006.10.008] [PMID]
28. Whitehead AG, Hemming JR (1965) A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology 55:25-38. [DOI:10.1111/j.1744-7348.1965.tb07864.x]
29. Xuehui L, Brumme EC (2012) Applied genetics and genomics in alfalfa breeding.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
