Volume 9, Issue 2 ((Spring and Summer) 2020)
Plant Pathol. Sci. 2020, 9(2): 14-27 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rostami A, Sadravi M, Rezaee R, ABDOLLAHI M. (2020). Biological control of Fusarium root rot of bean with two Trichoderma species and Pseudomonas fluorescens. Plant Pathol. Sci.. 9(2), 14-27. doi:10.52547/pps.9.2.14
URL: http://yujs.yu.ac.ir/pps/article-1-315-en.html
URL: http://yujs.yu.ac.ir/pps/article-1-315-en.html
Department of Plant Protection, Faculty of Agriculture, Yasouj University, Yasouj, Iran , m.sadravi@gmail.com
Abstract: (5601 Views)
Rostami A, Sadravi M, Rezaei R, Abdollahi M (2020) Biological control of Fusarium root rot of bean with two Trichoderma species and Pseudomonas fluorescens. Plant Pathology Science 9(2): 14-27. Doi: 10.2982/PPS.9.2.14
Introduction: Fusarium root rot with damage reported up to 85% of the crop yield, caused by Fusarium solani f. sp. phaseoli, is one of the most important bean diseases in the world. Biological control is a healthy and environmentally friendly way to manage this soil-borne disease. Materials and Methods: Bean farms in Kohgiluyeh and Boyer-Ahmad Province were visited and the rotten roots of diseased plants were sampled. Two isolates of the pathogen were isolated, purified and identified. The pathogenicity of these two isolates was tested on two bean varieties Drakhshan and Pak under greenhouse conditions. The colony growth inhibition rate of the hypervirulant isolate of the pathogen was assessed by 14 native isolates of Trichoderma harzianum, four isolates of Trichoderma virens, two isolates of Trichoderma atroviridae, and five native isolates of Pseudomonas florescens and P. florescens CHAO with hyperparasitic ability and production of antibiotics in vitro. Finally, the effect of four superior T. harzianum isolates, one T. atroviridae isolate and two P. florescens isolates on disease severity were examined in a completely randomized design in the greenhouse. Results: All isolates of three species of Trichoderma had the ability to hyperparasite and destroy pathogenic hyphae. Four T. harzianum isolates showed a more significant ability to produce non-volatile and volatile antibiotic materials. All treatments significantly reduced the disease severity, but a T. harzianum isolate was more effective in vivo. Conclusion: Fusarium root rot is also found in bean fields in southwestern Iran. Native isolates of T. harzianum, T. virens and T. atroviridae have the hyperparasitic ability on the pathogen. These fungi and isolates of P. florescens have the ability to inhibit the growth of the pathogen colony by producing antibiotic substances. Isolates of Trichoderma harzianum, T. atroviridae and P. florescens CHAO have the ability to reduce the severity of the disease in vivo.
References
1. Abeysinghe S (2007) Biological control of Fusarium solani f. sp. phaseoli the causal agent of root rot of bean using Bacillus subtilis CA32 and Tichoderma harzianum RU01. Ruhuna Journal of Science 2:82-88.
2. Ahl P, Voisard C, Défago G (1986) Iron bound‐siderophores, cyanic acid, and antibiotics involved in suppression of Thielaviopsis basicola by a Pseudomonas fluorescens strain. Journal of Phytopathology [DOI:10.1111/j.1439-0434.1986.tb00903.x]
3. Akbari A, Zafari DM, Rouhani H, Khodakaramian G (2006) Study of antagonistic activity of Bacillus species from bean rhizosphere against root rot caused by Fusarium solani f. sp. phaseoli. Agricultural Research 5(3):54-70.
4. Desai S, Reddy M S, Kloepper M S(2002). Comprehensive testing of biological agents. Pp: 387-420, In: S. S. Gnanamanickam (Ed.). Biological Control of Crop Diseases. Marcel Dekker Inc., New York, USA.
5. Ershad D (2009) Fungi of Iran. Iranian Research Institute of Plant Protection. Tehran, Iran, 558p.
6. Gharacheh N, Sadravi M (2015) Five important fungal diseases of pulse crops in Iran. Plant Pathology Science 4(2):17-25.
7. Hall R (1991) Compendium of Bean Disease. APS Press, USA.
8. Harman, G. E., Howell, C. R., Viterbo, A., Chet, I. and Lorito, M. 2004. Trichoderma species opportunistic, avirulent plant symbionts. Nat Rev Micro. 2: 43-56. [DOI:10.1038/nrmicro797] [PMID]
9. Harson, S., Schichler, H., Oppenhim, A., and Chet, I. (1996). Defferential expression of Trichoderma harzianum chitinases during mycoparasitism. Phytopathology. 86: 980-985. [DOI:10.1094/Phyto-86-980]
10. Hatamabadi Farahani M, Rezaei S, Lak MR (2010) The effect of Rhizobium leguminosarum strains on Fusarium root rot disease of bean. Agroecology Journal 5:1-8 (In Persian with English abstract).
11. Howell, C.R. (2003). Mechanism employed by Trichoderma species in biological control of plant diseases: The history and evolution of current concepts. Plant Disease: 87: 4-10. [DOI:10.1094/PDIS.2003.87.1.4] [PMID]
12. Khalili E, Sadravi M, Naeimi S , Khosravi V (2012) Biological control of rice brown spot with native isolates of three Trichoderma species. Brazilian Journal of Microbiology 43(1):297-305. [DOI:10.1590/S1517-83822012000100035]
13. Lak MR, Ghanbari A A, Dori HR, Ghadiri A (2009) Effect of planting date on seed yield and Fusarium root rot disease severity in chitti bean in Khomein. Seed and Plant Production Journal 25(3):271-282.
14. Leslie, J. F. and B. A. Summerell. (2006). The Fusarium Laboratory Manul. Blackwell Publishing. 387 pp [DOI:10.1002/9780470278376]
15. MirzaeiGhomi P, Zamanizadeh HR, SaberiRiseh RA, Lak MR (2010) Powder formulation survey of strain Bacillus subtilis M36 for control of bean root rot, caused by Fusarium solani f.sp. phaseoli. Iranian Journal of Plant Protection Science 41(1):151-163.
16. Parsa M, Bagheri A (2008) Legumes. JDM Press, Mashhad, Iran. (In Persian)
17. Ramamoorthy V, Raguchander T, Samiyappan R (2002) Induction of defense-related proteins in tomato roots treated with Pseudomonas fluorescens Pf1 and Fusarium oxysporum f. sp. lycopersici. Plant and Soil 239:55-68. [DOI:10.1023/A:1014904815352]
18. Valed Saravi Z, Sadravi M, Bahrami M (2011) Efect of three biological products on rice sheath blight disease in the field. Journal of Plant Protection 25(1):44-49 (In Persian with English abstract).
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
