Volume 9, Issue 2 (8-2020)                   pps 2020, 9(2): 1-13 | Back to browse issues page

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MIRZAPOUR S, DARVISHNIA M, bazgir E, mirzaei najafgholi H. Pathogenic variation of Didymella rabiei isolates causing Chickpea blight in three western provinces of Iran. pps. 2020; 9 (2) :1-13
URL: http://yujs.yu.ac.ir/pps/article-1-309-en.html
Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
Abstract:   (498 Views)
Mirzapour S, Darvishnia M, Bazgir E, Mirzaei Najafgholi H (2020) Pathogenic variation of Didymella rabiei isolates causing Chickpea blight in three western provinces of Iran. Plant Pathology Science 9(2):1-13. DOI: 10.2982/PPS.9.2.1
 
Introduction: Chickpea blight, caused by Didymella rabiei, is the most limiting factor in chickpea production areas in the world, including the western provinces of Iran. The aim of this study was to investigate the pathogenic diversity of the isolates collected from western provinces of Iran (Ilam, Lorestan and Kermanshah) on differential lines of chickpea, as well as the relationship between pathogenic diversity of the isolates and their geographical origins. Materials and Methods: During the 2017-18 chickpea growing season, samples of infected plants were collected from fields in western provinces and transferred to the laboratory. Then 100 pure isolates of D. rabiei were obtained. Based on the location of collection, the isolates were divided into 20 groups. Based on the morphological characteristics, one isolate from each group was selected as a representative for greenhouse experiments. The pathogenic diversity of theses 20 isolates was assessed on eight differential lines of chickpea. The factorial experiments were performed in a completely randomized block design under greenhouse conditions. Results: The results of analysis of variance showed that there was a significant difference between differential lines and isolates at a probability level of p<0.01. Based on the response of the differential lines, the pathogenic isolates were grouped into three pathogenic groups, including pathogenic group 1 (weakly aggressive), group 2 (aggressive or moderately aggressive), and group 3 (highly aggressive). The isolates from Kermanshah and Lorestan Province were classified into Group 1 and the Ilam isolates into Groups 1, 2 and 3. The greatest severity of the disease was found in three isolates of Ilam province. The variety ILC3996 showed the highest resistance to all isolates. Conclusion: D. rabiei isolates from three western provinces of Iran are divided into three pathogenic groups: 1 (low aggressive), 2 (moderately aggressive), and 3 (highly aggressive). Highly aggressive isolates identified in Ilam province can be used in chickpea breeding programs to produce resistant cultivars to the disease.. The wild variety ILC3996 has resistant genes against these pathogenic groups and showed resistance to all of these isolates.
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Type of Study: Research | Subject: Special

References
1. Atik O, Seid A, Abang MM, Imtiaz M, Hamwieh A, Baum M, El-Ahmed A, Murad S, Yabrak MM (2013) Pathogenic and genetic diversity of Didymella rabiei affecting chickpea in Syria. Crop Protection 46:70-79 [DOI:10.1016/j.cropro.2012.12.012]
2. Baite MS, Dubey S, Singh B (2016) Morphological variability in the Indian isolates of Ascochyta rabiei causing blight in chickpea and evaluation of chickpea cultivars. Indian Journal Plant Protection 44:74-82.
3. Barnet HL, Hunter BB (1972) Illustrated Genera of Imperfect Fungi. Burgess Publishing Company, Minneapolis, USA, 241p.
4. Chen W, Coyne CJ, Peever TL, Muehlbaur FJ (2004) Characterization of chickpea differentials for pathogenicity assay of Ascochyta blight and identification of chickpea accessions resistant to Didymella rabiei. Plant Pathology Journal 53:759-769. [DOI:10.1111/j.1365-3059.2004.01103.x]
5. Chongo G, Gossen B, Buchwaldt L, Adhikari T, Rimmer S ( 2004) Genetic diversity of Ascochyta rabiei in Canada. Plant Disease 88:4-10. [DOI:10.1094/PDIS.2004.88.1.4]
6. Dolar FS, Tenuta A, Higgins VJ (1994) Detached leaf assay for screening chickpea for resistance to Ascochyta blight. Canadian Journal of Plant Pathology 16:215-220. [DOI:10.1080/07060669409500756]
7. Gharacheh N, Sadravi M (2015) Five important fungal diseases of pulse crops in Iran. Plant Pathology Science 4(2):17-25.
8. Ghiai S, Razavi M, Shahriyari D (2011) Study on pathogenic and molecular variability in some isolates of Ascochyta rabiei causal agent of Ascochyta blight of chickpea in Iran. Entomology and Phytopathology Journal 79:199-218. (In Persian with English Abstract).
9. Hamwieh A, Imtiaz M, Hobson K, Kemal SA (2013) Genetic diversity of microsatellite alleles located at quantitative resistance loci for Ascochyta blight resistance in a global collection of chickpea germplasm. Phytopathology Mediterranea 52:183−191.
10. Hina A, Alam SS, Nayyer I (2013). Genetic and pathogenic variability of Ascochyta rabiei isolates from Pakistan and Syria as detected by universal rice primers. Journal of Plant Pathology and Microbiology 4:212.
11. Imtiaz M, Abang MM, Malhotra R S, Ahmed S, Bayaa B, Udupa SM, Baum M (2011) Pathotype IV, a new and highly virulent pathotype of Didymella rabiei, causing Ascochyta blight in chickpea in Syria. Plant Disease 95:1192-1192. [DOI:10.1094/PDIS-04-11-0333]
12. Jamil FF, Sarwar N, Sarwar M, Khan JA, Geistlinger J, Kahl G (2000) Genetic and pathogenic diversity within Ascochyta rabiei (Pass.) Lab. populations in Pakistan causing blight of chickpea (Cicer arietinum L.). Physiological and Molecular Plant Pathology 57:243-254. [DOI:10.1006/pmpp.2000.0303]
13. Mahiout D, Bendahmane BS, Benkada MY, Rickauer M (2015) Physiological Characterisation of Ascochyta rabiei (Pass.) Lab. Isolated from diseased chickpea Fields in Six Regions of Northwestern Algeria. American-Eurasian Journal Agriculture & Environment Science 15:1136-1146.
14. Mehmood Y, Sambasivam P, Kaur S, Davidson J, Leo AE, Hobson K, Linde C C, Moore K, Brownlie J, Ford R (2017). Evidence and consequence of a highly adapted clonal haplotype within the Australian Ascochyta rabiei population. Frontiers in Plant Science, 8:1029. [DOI:10.3389/fpls.2017.01029]
15. Mel'nik VA, Braun U, Hagedorn G (2000) Key to the Fungi of the Genus Ascochyta Lib. (Coelomycetes). Biologische Bundesanstalt für Land- und Forstwirtschaft, Berlin, Germany,192p.
16. Nourollahi K, Javannikkhah M, Naghavi MR, Okhovat SM (2009) Pathogenic diversity in Didymella rabiei from the western Iranian Ilam and Kermanshah provinces. Journal of Plant Protection 23:56-65. (In Persian with English Abstract).
17. Pande S, Sharma M, Gaur PM, Tripathi S, Kaur L, Basandrai A, Khan T, Gowda CLL, Siddique KHM (2011) Development of screening techniques and identification of new sources of resistance to Ascochyta blight disease of chickpea. Australasian Plant Pathology 40:149-156. [DOI:10.1007/s13313-010-0024-8]
18. Paymard A, Torabi M, Shahryari D (2014). Pathogenic variability of Didymella rabiei the agent of ascochyta blight of chickpea in Kermanshah province. Applied Plant Protection 3:73-85. (In Persian with English Abstract).
19. Peever T, Chen W, Abdo Z, Kaiser W (2012) Genetics of virulence in Ascochyta rabiei. Plant Pathology 61:754-760 [DOI:10.1111/j.1365-3059.2011.02566.x]
20. Pouralibaba H, Mahmodi F, Keshavarz K, Nourollahi KH (2008) Identification of pathotypes of Didymella rabiei causing agent of chickpea blight disease in different parts of Iran using trap nursery. Journal of Plant Pathology 44:170-175. (In Persian with English Abstract).
21. Rahimi M, Sabbagh SK, Nikkhah MJ, Salari M, Panjekeh N (2013) Study of genetic diversity of A. rabiei the causal agent of chickpea Ascochyta blight by SSR marker in Lorestan province. Journal of Plant Protection Science 44:273-282. (In Persian with English Abstract).
22. Sharma M, Ghosh R (2016) An update on genetic resistance of chickpea to ascochyta blight. Agronomy 6:18. [DOI:10.3390/agronomy6010018]
23. Shokouhifar F, Bagheri A, Falahati RM, Malekzadeh S (2003) Pathotyping of Ascochyta rabiei isolate in Iran. Journal of Agricultural Sciences and Natural Resources 1:217-232. (In Persian with English Abstract).
24. Tekin M, Sari D, Catal M, Ikten C, Smykal P, Penmetsa RV (2017) Eco-geographic distribution of Cicer arietinum PH Davis and threats to the species. Genetic Resources and Crop Evolution 65:67-77 [DOI:10.1007/s10722-017-0509-1]
25. Turkkan M, Dolar FS (2009) Determination of pathogenic variability of D. rabiei, the agent of ascochyta blight of chickpea in Turkey. Turkish Journal of Agriculture and Forestry 33:585-591.
26. Vafaei SH (2019) Blight disease of chickpea. Journal of Plant Pathology 8:45-57. (In Persian with English Abstract). [DOI:10.29252/pps.8.2.45]
27. Vafaei SH, Rezaee S, Abasi Moghadam A, Zamanizadeh H R (2015) Virulence diversity of A. rabiei the causal agent of Ascochyta blight of chickpea in the western provinces of Iran. Archives of Phytopathology and Plant Protection 48:921-930. [DOI:10.1080/03235408.2016.1143600]
28. Vail S, Banniza S (2008) Structure and pathogenic variability in Ascochyta rabiei populations on chickpea in the Canadian prairies. Plant Pathology 57:665-673. [DOI:10.1111/j.1365-3059.2008.01837.x]
29. Younesi H, Okhovvat SM, Hadjaroude GA, Zad SJ, Talei AR, Zamani M (2003) Virulence variability of Ascochyta rabiei isolates on chickpea cultivars in Kermanshah province. Journal of Plant Pathology 39:213-228. (In Persian with English Abstract).

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