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Showing 2 results for Naseri

Bita Naseri,
Volume 5, Issue 2 (8-2016)
Abstract

Naseri  B. 2016. Integrated  management of Rhizoctonia root rot of bean. Plant Pathology Science 5(2):42-51.

Rhizoctonia root rot caused by Rhizoctonia solani Kühn seriously reduces bean yield. Restricting wide distribution of the disease in main bean growing regions requires identification of factors effect on the disease occurrence and prevalence. Due to the lack of resistant cultivars and ineffective chemical control measures, cultural practice management plays an important role in disease control. According to the findings, increasing soil organic matter, improving rhizoobial nodule formation on root, not planting beans in sandy soils, maintaining nuteral pH of field soil, following 7-9 days irrigation interval throughout growing season, planting standard density of 30 plants per square meter, seeding at less than five cm depth under warm and dry climatic conditions, using sprinkler irrigation, growing red bean in infected fields, appropriate rotation program, applying maximum 50 kg/ha urea, weed control, and seed treatment with proper systemic fungicide should be considered in an integrated management program.


Masoumeh Vakili-Ghartavol, Hossein Arouiee, Dr. Shiva Golmohammadzadeh, Mahboobeh Naseri,
Volume 11, Issue 2 ((Spring and Summer) 2022)
Abstract

Vakili-Ghartavol M, Arouiee H, Golmohammadzadeh S, Naseri M (2022) Optimal methods for the preparation of fungal mycelium for examination with the scanning electron microscope. Plant Pathology Science 11(2):42-51.  
 Doi: 10.2982/PPS.11.2.42
 
Introduction: Plant-pathogenic fungi are considered a serious threat to world food security, causing spoilage in plant products and food poisoning. One of the ways to identify fungi is to examine their mycelium using a scanning electron microscope (SEM). In order to take high-resolution microscopic images and obtain the surface properties of mushroom mycelium samples, it is very important to prepare the samples, i.e. the method of dehydrating and drying them. Materials and Methods: In this research, the effect of two dehydration methods and two drying methods on the mycelia of two fungi, Rhizoctonia solani and Rhizopus stolonifer, were compared to study them with SEM. Results: Microscopic observations showed that for R. stolonifer, dehydration with 100% ethanol and drying and for R. solani at room temperature and dehydration with 50% ethanol and the freezing method produce a smooth and high-resolution image compared to other methods. Conclusion: Dehydration of fungal mycelium with 50% to 100% ethanol and drying at room temperature or freezing are the optimal methods to prepare them for examination with SEM and produce a smooth and high-resolution image, and the surface features of their mycelium are preserved and well-defined.


 

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