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Former Ms.c. Saeed Sharify Rostam-Abady, Dr. Mahdieh Rostami, Dr. Farahnaz Jahanshahi Afshar, Fariba Ardeshir,
Volume 12, Issue 1 (3-2023)
Volume 12, Issue 1 (3-2023)
Abstract
Sharify Rostam-Abadi, S., Rostami, M., Jahanshahi Afshar, F., Ardeshir, F. (2023). Three plant-parasitic nematodes of the family Longidoridae from orchards of Rafsanjan county, Iran. Plant Pathology Science 12(1),12-24.
Introduction: Longidoridae is a family of plant-parasitic nematodes belonging to the order Dorylaimida, that cause damage to most agricultural crops especially fruit trees by direct damage and also transmitting some plant pathogenic viruses. Considering the lack of prior research studies on this topic in the fruit orchards of Rafsanjan region, identifying and monitoring their population is important. Materials and Methods: In a study that was conducted to identify plant-parasitic nematodes of horticultural products in Rafsanjan city, some soil samples were collected from the rhizosphere of various fruit trees, in 2020. The nematodes were extracted from the soil samples using two methods, centrifugal flotation-sieving and tray technique, fixed and transferred to the anhydrous glycerin. After preparing permanent slides, the nematodes were studied using a light microscopy. The nematode species were identified based upon morphological and morphometric data using relevant valid references. Results: In this study, three species of Dorylaimida order belonging to the Longidoridae family including the two genera: Longidorus (L. africanus) and Xiphinema (X. vuittenezi and X. index) were obtained from the rhizosphere. Also, in order to confirm the traditional identification of X. vuittenezi, this species was molecularly studied using D2-D3 extension fragment of LSU rDNA. The obtained sequence of the species was 100% identical to the sequences of the other populations of X. vuittenezi in the GenBank. Conclusion: This study shows that regional nematode population monitoring needs more attention.
Aminallah Tahmasebi,
Volume 12, Issue 1 (3-2023)
Volume 12, Issue 1 (3-2023)
Abstract
Tahmasebi, A. (2023). The role of translation initiation factors in plants recessive resistance to viruses. Plant Pathology Science, 12(1), 113-121.
Abstract
Plant viruses are important pathogens that cause quantitative and qualitative decline of agricultural products all over the world. Plants resistance is the most effective way to control plant viruses. Viruses as obligate parasites to complete their infection cycle, such as the processes of protein synthesis, replication, and movement, are dependent on the compatibility of cellular factors of host plants. Absence or mutation in these essential factors for the virus infection cycle or mutation in the regulator of plant defense responses may cause the host's recessive resistance to the virus. Recessive genes identified in virus-plant interactions include eukaryotic translation initiation factors eIF4E, eIF4G, and their isoforms. A number of translation factors have been identified in plants, such as eIF3, eEF1A, and eEF1B, which are essential in interacting with viral RNAs and regulating various processes in the virus infection cycle. More awareness of molecular mechanisms of these factors as well as their interaction with other host and viral factors can be used in the development of new management methods such as silencing or genome editing against viruses.
Fereshteh Esmaeilzadeh, Dr. Davoud Koolivand,
Volume 12, Issue 1 (3-2023)
Volume 12, Issue 1 (3-2023)
Abstract
Esmaeilzadeh, F., & Koolivand, D. (2022). Tomato brown rogue fruit virus. Plant Pathology Science 12(1), 84-93.
Tomato brown rugose fruit virus (ToBRFV) is an emerging pathogen that generally causes disease in greenhouses. This virus causes yellow areas, malformation and brown necrotic lesions on tomato fruits. This virus, which has a global distribution, has recently been reported using molecular methods in the greenhouses of few provinces of Iran. According to the report of this virus from Iran, the characteristics of the ToBRFV, its symptoms, host range, mode of transmission and spread, and its management methods have been described based on previous studies.
Arman Kamgar, Saghar Ketabchi,
Volume 12, Issue 1 (3-2023)
Volume 12, Issue 1 (3-2023)
Abstract
Kamgar, A. & Ketabchi, S. (2023). The effect of three biofertilizers on growth indices of tomato and severity of bacterial wilt disease. Plant Pathology Science 12(1), 105-112.
Introduction: Bacterial wilt caused by Ralstonia solanacearum is one of the major diseases of tomato in the world. Today, new methods based on the use of biological products for the management of plant diseases have been considered to reduce the consumption of chemical toxins and damage to the environment, to provide an organic product for consumers. Materials and Methods: The effect of three biofertilizers: Nitro Kara (containing several nitrogen-fixing bacteria), effective microorganisms (EM®: containing several species of yeasts and bacteria), and compost on tomato growth indices and severity of bacterial wilt disease were evaluated in a completely randomized design in greenhouse conditions. Results: Analysis of variance showed that, these three bio-fertilizers had significant effect on increasing tomato growth indices, but did not significantly reduce the disease severity. Compost among them had the greatest effect on tomato growth indices even in the presence of the pathogen. Conclusion: Compost can be considered as an improvement factor for tomato growth indices in the management program of bacterial wilt disease.
Zahereh Panahi, Reza Khakvar, Naser Aliasgharzad, Saeed Zehtab, Reza Farshabf Pourabad,
Volume 12, Issue 1 (3-2023)
Volume 12, Issue 1 (3-2023)
Abstract
Panahi, Z., Khakvar, R., Aliasgharzad, N., Zehtab, S., & Farshbaf PourAbad, R. (2023). The effect of copper nanoparticles on soft rot agent of potato, carrot and onion. Plant Pathology Science, 12(1), 1-11.
Introduction: Bacterial soft rot caused by Pectobacterium species is one of the important and common diseases in Potatoes and vegetables. Disinfection of tubers or seeds with chemicals is one of the methods of disease management. Copper nanoparticles, like silver and gold nanoparticles, have a strong inhibitory effect on bacterial cells, but they are much cheaper and more accessible than them. This research was conducted to determine the effect of copper nanoparticles alone and in combination with oxytetracycline and streptomycin antibiotics on potato, carrot and onion soft rot. Materials and Methods: The pathogen was isolated from rotten tissues of potato, carrot and onion, purified and identified by studying the phenotypic and genetic characteristics of the Pel-gene region using Pectobacterium specific primers (Y1 and Y2). The effect of copper nanoparticles, oxytetracycline and streptomycin, and their combination with copper nanoparticles on the pathogen growth was investigated in a completely randomized design experiment with three replications for each treatment in vitro. Results: Pectobacterium odoriferum was identified as pathogen based on phenotypic characteristics and genetic affinity. Streptomycin compared to oxytetracycline inhibited the pathogen growth more and their effect in combination with copper nanoparticles increased by 12 and 19.5%, respectively. Conclusion: Copper nanoparticles can inhibit the growth of P. odoriferum, and in combination with antibiotics increase their effect.
Mahdi Akhlaghi, Mohammad Reza Alymanesh, Abbas Rohani, Ali Reza Barjasteh, Mohammad Abedini Esfahlani, Ahmad Dezyania,
Volume 12, Issue 1 (3-2023)
Volume 12, Issue 1 (3-2023)
Abstract
Akhlaghi, M., Alymanesh, MR., Rohani, A., Barjasteh, A., and Abedini Esfahlani, M., & Dezianian, A. (2023). The impact of two active ingredients of plant essential oils on the causative agent of apple and pear fire blight disease. Plant Pathology Science, 12(1), 53-63. Introduction: Erwinia amylovora, the cause of apple and pear fire blight disease, is one of the main factors limiting the production of these fruits in the world. Since only a limited number of copper compounds have a protective effect in controlling this disease, increasing attention has been paid to investigating the effect of plant essential oils on this pathogen. Materials and Methods: The effect of different concentrations of carvacrol (the active ingredient of thyme, oregano, etc.) and trans-anethole (the active ingredient of fennel essential oil) on the growth of E. amylovora colony, was investigated in laboratory conditions, and their minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined. The inhibitory effect of MIC of these substances alone and the mixture of one-half of their MIC and MBC concentrations was investigated by disc method on nutrient agar medium on the growth of E. amylovora colony. The effect of the three best mixing ratios of these materials on the fruit tissue of the pear Spadona variety was evaluated on the severity of fire blight disease. Results: Carvacrol and trans-anethole both had antibacterial effects against E. amylovora, but the effect of carvacrol was greater than the other. Mixing carvacrol with trans-anethole increased its effectiveness. Among the three mixing ratios, 47 µg/ml of carvacrol + 141 µg/ml of trans-anethole caused the greatest reduction in disease severity in pear fruit tissue. Conclusion: The use of a mixture of carvacrol and trans-anethole can be considered for the management of fire blight disease.
Leila Rasouli , Mehdi Sadravi , Kavoos Keshavarz ,
Volume 12, Issue 1 (3-2023)
Volume 12, Issue 1 (3-2023)
Abstract
Rasouli, L., Sadravi, M., & Keshavarz, K. (2023). Screening eight tomato varieties for resistance to Fusarium wilt disease. Plant Pathology Science, 12(1), 46-52.
Introduction: Fusarium wilt caused by soil-borne fungus Fusarium oxysporum f. sp. lycopersici (Fol) is one of the most important diseases of tomatoes in the world. Identification and cultivation of resistant varieties is an environmentally friendly method of disease management that leads to the production of a chemical-free and clean yield. This research was conducted to evaluate the reactions of eight tomato varieties to the disease and their correlation with peroxidase enzyme. Materials and Methods: Wilted tomato plants were sampled in the greenhouses of southwestern Iran and after isolation and purification and studying the morphological characteristics, two Fol isolates were identified. The reactions of eight varieties vs. Kingston, Dafnis, Super chef, Karnak, CHpooya, Earlypooya, Superpooya, and CH to these two isolates were investigated in a factorial experiment with a completely randomized statistical design with four replications for each treatment under greenhouse conditions. The disease severity index and the vegetative and reproductive indices of the plant were determined. The level of peroxidase enzyme in the leaves of plants of each treatment was measured by spectrophotometric method at 470 nm wavelength and its correlation coefficient was determined with the disease severity index. Results: Analysis of the variance of these experimental data revealed a significant difference between Fol isolates in pathogenicity and interaction between Fol isolates and varieties. Fol2 isolate was more aggressive than Fol1 and CH was moderately susceptible, and Earlypooya, Karnak, Superpooya, Dafnis, CHpooya, were moderately resistant, while Kingston and Super chef were completely resistant to this hyper-virulent isolate of the pathogen and disease. The peroxidase level had negatively correlated with the disease severity index. Conclusion: Among these varieties, Kingstone and Super chef are resistant to the disease. Peroxidase levels can be used as a resistance marker to assess how different tomato varieties react to the disease.
Report of six plant parasitic nematodes from the orchards of Rafsanjan region, Kerman Province, Iran
Former Ms.c. Saeed Sharify Rostam-Abady, Mahdieh Rostami, Farahnaz Jahanshahi Afshar, ,
Volume 12, Issue 2 (9-2023)
Volume 12, Issue 2 (9-2023)
Abstract
Sharify Rostam-Abadi S, Rostami M, Jahanshahi Afshar F, Ardeshir F (2023) Report of six plant parasitic nematodes from the orchards of Rafsanjan region, Kerman Province, Iran. Plant Pathology Science 12(2):41-52.
Introduction: In the suburban area of Rafsanjan city in Kerman Province, Iran, there are many orchards infested with plant parasitic nematodes. Therefore, it was necessary to identify the plant parasitic nematodes in the orchards of this region. Materials and Methods: In 2018 and 2019, some soil samples were taken around the roots of apricot, peach, pomegranate, apple, cherry and persimmon trees in this area. Nematodes were extracted by two methods, tray and sieve-centrifuge, and after fixation, they were transferred to pure glycerin. Permanent slides were examined with a bright field microscope and extracted nematodes were identified using diagnostic keys and original descriptions. Results: Seven nematodes from the infraorder Tylenchomorpha, belonging to five families including: Tylenchidae, Pratylenchidae, Tylenchulidae, Criconematidae and Anguinidae were identified. Root-lesion nematode (Pratylenchus penetrans), pin-nematode (Paratylenchus neoamblycephalus) and Criconematid (Mesocriconema xenoplax) were the most important plant parasitic nematodes in fruit orchards of this region. Conclusion: Due to the presence of some important and damaging nematodes in the orchards of Rafsanjan, it is recommended to implement appropriate management methods and monitor the population of important plant parasitic nematodes.
Morteza Bavand, Mehdi Sadravi, Habiballah Charehgani,
Volume 12, Issue 2 (9-2023)
Volume 12, Issue 2 (9-2023)
Abstract
Bavand, M., Sadravi, M., & Charehgani, H. (2023). Screening of fifteen tomato varieties for resistance to early blight disease. Plant Pathology Science 12(2),1-10.
Introduction: Early blight caused by Alternaria species is one of the major tomato diseases worldwide, causing losses of up to 86% of yield. Identifying and cultivating resistant varieties is the best method for disease management. Considering the prevalence and importance of diseases in Iran, this study was conducted to identify resistant cultivars among 15 available varieties. Materials and Methods: In Kohgiluyeh and Boyer-Ahmad province in southwestern Iran, a severely affected tomato field by the disease was visited and samples of the diseased plants were taken. The pathogenic fungus was isolated on potato dextrose agar medium from the diseased tissues of the plant after their surface disinfection and after its purification by the single spore method and its morphological properties were examined and measured. The collected data were compared with the descriptions of Alternaria species and based on that, the pathogen was identified. Seedlings of 15 tomato varieties were inoculated with a conidia suspension at the 4-leaf stage in a completely randomized statistical design and maintained in a growth chamber with a temperature of 28oC, relative humidity of 90%, and a photoperiod of 16 hours of light and eight hours of darkness. Eighteen days after inoculation, the response of each cultivar to the disease was determined by calculating the percentage of infected leaves as well as the number and size of spots, and the disease index. Results: Alternaria alternata has been identified as the cause of early blight in tomatoes in this region. Varieties 10552, King Stone, Super Chief and Ventero with minimal symptoms were scored as resistant; 4129, 3725, 4224, Retino, Aras, Flat 111 and Super Pooya as semi-resistant and 7806, Early Pooya, 11057 and CH Pooya were known to be susceptible to the disease. Conclusion: Cultivation of resistant or semi-resistant varieties can be recommended for disease control. The occurrence of tomato blight disease caused by A. alternata is reporting here for the first time from the region.
Introduction: Early blight caused by Alternaria species is one of the major tomato diseases worldwide, causing losses of up to 86% of yield. Identifying and cultivating resistant varieties is the best method for disease management. Considering the prevalence and importance of diseases in Iran, this study was conducted to identify resistant cultivars among 15 available varieties. Materials and Methods: In Kohgiluyeh and Boyer-Ahmad province in southwestern Iran, a severely affected tomato field by the disease was visited and samples of the diseased plants were taken. The pathogenic fungus was isolated on potato dextrose agar medium from the diseased tissues of the plant after their surface disinfection and after its purification by the single spore method and its morphological properties were examined and measured. The collected data were compared with the descriptions of Alternaria species and based on that, the pathogen was identified. Seedlings of 15 tomato varieties were inoculated with a conidia suspension at the 4-leaf stage in a completely randomized statistical design and maintained in a growth chamber with a temperature of 28oC, relative humidity of 90%, and a photoperiod of 16 hours of light and eight hours of darkness. Eighteen days after inoculation, the response of each cultivar to the disease was determined by calculating the percentage of infected leaves as well as the number and size of spots, and the disease index. Results: Alternaria alternata has been identified as the cause of early blight in tomatoes in this region. Varieties 10552, King Stone, Super Chief and Ventero with minimal symptoms were scored as resistant; 4129, 3725, 4224, Retino, Aras, Flat 111 and Super Pooya as semi-resistant and 7806, Early Pooya, 11057 and CH Pooya were known to be susceptible to the disease. Conclusion: Cultivation of resistant or semi-resistant varieties can be recommended for disease control. The occurrence of tomato blight disease caused by A. alternata is reporting here for the first time from the region.
Masoumeh Delaramifar, Mahdi Pirnia, Mojtaba Keykhasaber, Shirahmad Sarani, Hamideh Khajeh,
Volume 12, Issue 2 (9-2023)
Volume 12, Issue 2 (9-2023)
Abstract
Delaramifar M, Pirnia M, Keykhasaber M, Sarani SA, Khajeh H (2023) Reaction of eight luffa genotypes to damping-off disease. Plant Pathology Science 12(2):76-85.
Introduction: Damping-off caused by Pythium aphanidermatum is one of the major diseases of luffa. Identifying and planting of resistant varieties is an environmentally friendly solution for integrated disease management. This study was conducted to determine the reaction of eight native and non-native luffa genotypes to the disease. Materials and Methods: The pathogen (Pythium aphanidermatum IRAN597C) was obtained from the collection of fungi of the Iranian Institute of Plant Protection Researches. It was inoculated into seedlings of eight luffa genotypes. After the appearance of yellowing symptoms and seedling death, the disease index (DI) and the area under the disease progression curve (AUDPC) were calculated for each genotype. Koch's postulates were carried out to prove pathogenicity and the pathogen was isolated from diseased seedlings. Then, for molecular confirmation of the pathogen, ITS-rDNA sequencing was used. Results: The sequencing of the ITS-rDNA region of the pathogen showed a phylogenetic affinity of 99% with other isolates of P. aphanidermatum. According to the DI, the northern large and the long luffa genotypes were grouped as sensitive genotypes, and other genotypes including northern black seed, northern white seed, toori, Afghani, grooved and Brazilian were grouped as resistant genotypes. Based on the AUDPC, the northern black seed, and northern white seed genotypes showed the lowest level, toori, grooved, Afghani and Brazilian genotypes showed the medium level, and northern large and long luffa genotypes showed the highest AUDPC level. Conclusion: Considering the low values of the DI and AUDPC in the northern black seed and northern white seed genotypes, planting these two genotypes is suggested for management of the disease in luffa.
Azadeh Goudarzi,
Volume 12, Issue 2 (9-2023)
Volume 12, Issue 2 (9-2023)
Abstract
Goudarzi A (2023) Black scorch disease of date palm. Plant Pathology Science 12(2):66-75.
Black scorch disease is considered one of the important and damaging diseases of date palm in many regions of the world, including Oman, Iraq, Qatar, Saudi Arabia, Kuwait, and the United Arab Emirates. The disease was first reported in Iran in 2014 from Tabas in South Khorasan Province, and in the following years, symptoms of the disease were observed scattered in Kerman, Hormozgan, Fars and Bushehr provinces. Two fungi, Thielaviopsis paradoxa and T. radicicola, are known as causal agents of the disease. Disease symptoms include tissue dieback, wilting, canopy bowing, terminal meristem rot, and tree dieback. The disease is commonly observed on trees suffering from environmental stress, particularly salt and drought stress. Although the susceptibility of different cultivars to the disease varies, the disease has been reported in 21 date palm cultivars worldwide to date. This article describes integrated disease management methods, including preventing diseases from entering the area, preventing damage to date palms, adhering to plant health principles, managing environmental stress, and proper ventilation in the grove, as well as biological and chemical control.
Saeedeh Dehghanpour Farashah,
Volume 12, Issue 2 (9-2023)
Volume 12, Issue 2 (9-2023)
Abstract
Dehghanpour Farashah S (2023) Defense responses by nitric oxide in plant-pathogen interaction. Plant Pathology Science 12(2):130-142.
The control of diseases in agriculture often relies on pesticides and chemical fertilizers, which negatively affect the rhizosphere natural microflora and ecosystem balance. Today, researchers are looking to replace these chemicals with other environment friendly agents to improve agricultural production and control plant pathogens. Research on the interaction of nitric oxide (NO) with plant pathogens has shown that NO is a key messenger in the response of plants to biotic and abiotic stresses. Due to the role of NO in the regulation of plant defense genes, especially through programmed cell death, it has attracted the attention of many plant pathologists. Although NO plays an important role in the hypersensitive reaction in response to various biotic and abiotic stresses, it has been found that NO also plays a role in regulating the expression of genes related to non-specific resistance. In this review, the role of nitric oxide in plant-pathogens interaction has been investigated.
Maryam Mousivand,
Volume 13, Issue 1 (2-2024)
Volume 13, Issue 1 (2-2024)
Abstract
Mousivand, M. (2024). New technologies for detecting mycotoxins in plant yields and products. Plant Pathology Science, 13(1), 89-103.
Mycotoxins are fungal secondary metabolites known as global concerns on humans and livestock health regarding to their toxicity and carcinogenicity. Increasing demand for fast, simple and low-cost detection of these compounds, especially on-site, have been lead to develop various biosensors. Although antibodies have been the most widely used diagnostic probes in biosensors for several decades but monoclonal antibody production is difficult for mycotoxins as small and non-immunogenic molecules. Therefore, aptameric probes have been emerged as a new technology for mycotoxin monitoring. Aptamers are single-stranded oligonucleotides that detect target molecule by folding into a three-dimensional conformation, with a binding affinity equal to that of monoclonal antibodies. Aptamers have been considered as the most important competitors of antibodies for biosensor development regarding to their significant advantages in terms of no limiting in target type, smaller size, higher stability, synthetic nature and low cost. Exploiting aptameric probes in biosensor designing have been led to aptasensors development for specific and sensitive tracking of target molecules, and mycotoxins. The aptameric probes, experimental screening methodology, various aptasensors and their applications in detecting mycotoxins has been described, and discussed in this article.
Mohammad Ali Hooshyar, Mehdi Sadravi, Rasool Rezaee ,
Volume 13, Issue 2 (9-2024)
Volume 13, Issue 2 (9-2024)
Abstract
Rhizoctonia root rot, caused by the soil-borne fungus Rhizoctonia solani, is an important disease of beans, which has been reported from different parts of Iran. The disease has been reported to cause damage to up to 60% of the crop worldwide. Biological control can be a healthy and environmentally friendly method for managing plant diseases. This study was conducted to investigate the effect of four commercial biological products available in the Iranian market on growth indices and severity of Rhizoctonia root rot in beans to find a suitable method for biological control of the disease. The effect of three biological products of arbuscular mycorrhizal fungi including Funeliformis mosseae, Rhizoglomus intraradices and Mycopersica (a mixture of several mycorrhizal fungi) and the bacterium Bradyrhizobium japonicum on growth indices and severity of Rhizoctonia root rot in Kosha pinto-bean cultivar was tested under greenhouse conditions. Statistical analysis of the data from this experiment showed that these treatments had a significant effect on reducing disease severity and plant growth indices, and among them, F. mosseae and Mycopersica caused the greatest reduction in disease severity and improved plant growth indices, respectively. Therefore, the biological product of F. mosseae and Mycopersica can be used to reduce the severity of Rhizoctonia root rot disease in beans and improve its growth indices.
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