Samaneh Samavat, Mahdiyeh Salehi Vozhdehnazari, Pegah Sayyad-Amin,
Volume 11, Issue 1 (3-2022)
Abstract
Samavat S, Salehi Vozhdehnazari M, Sayyad-Amin P (2022) Inhibitory effects of essential oils and extracts of medicinal plants on plant pathogenic fungi and bacteria. Plant Pathology Science 11(1):113-121. Doi: 10.2982/PPS.11.1.113.
The use of essential oils and extracts of medicinal plants is one of the environmentally friendly methods in the management of plant diseases caused by fungi and bacteria. The antimicrobial effect of essential oils and extracts of various medicinal plants such as thyme, cloves, savory, garlic, licorice and fennel has been proven and their active ingredient has also been identified. Based on these studies, the possibility of introducing, formulating and using essential oils and extracts of medicinal plants or any of their components with antimicrobial properties, as an alternative method of using chemical toxins in the management of fungal and bacterial diseases of plants can be provided.
Parmida Aleahmad, Leila Ebrahimi,
Volume 11, Issue 1 (3-2022)
Abstract
Aleahmad P. Ebrahimi L (2022) The role of endophyte fungi in plant disease management. Plant Pathology Science 11(1):100-112.
Doi: 10.2982/PPS.11.1.100.
Plant diseases usually cause economic damage and reduce growth and crop yield. Treatment of plant diseases with chemical toxins can result in environmental side effects, the emergence of resistance in pathogens, and loss of beneficial soil microorganisms, so the search for alternative methods such as identification and use of antagonistic microorganisms for disease treatment has been considered by researchers. A set of endophyte fungi that grow without damage or symptoms in host plant tissues and are known to be biological control agents of some plant diseases. Endophyte fungi with different mechanisms such as the production of antibiotic metabolites, competition, induction of resistance, and hyperparasitism of the pathogen weaken or eliminate the pathogens. These fungi can also help increase the growth and resistance of host plants to various stresses by producing antioxidant and antitumor compounds, as well as various growth hormones.
Hadis Shahbazi, Alireza Tarang, Fereidoun Padasht, Maryam Hosseini Chaleshtari, Mehrzad Allahgholipour, Maryam Khoshkdaman, Seyyede Akram Mousavi Qale Roudkhani, Sousan Nazari Tabak, Farzaneh Asadollahi Sharifi, Mahnaz Pourabbas,
Volume 11, Issue 1 (3-2022)
Abstract
Shahbazi H, Tarang A, Padasht F, Hosseini Chaleshtari M, Allah-Gholipour M, Khoshkdaman M, Mousavi Qaleh Roudkhani SA, Nazari Tabak S, Asadollahi Sharifi F, Pourabbas Dolatabad M (2022) The reaction of 109 rice lines to blast disease. Plant Pathology Science 11(1):24-35. Doi: 10.2982/PPS.11.1.24.
Introduction: Blast caused by Pyricularia oryzae is the most important fungal disease of rice in the world. The best method of disease management is to identify and cultivate resistant cultivars. Materials and Methods: In this study, the response of 109 promising lines from the Iranian Rice Research Institute along with three control cultivars (susceptible and resistant) to blast disease was evaluated. Seeds of each line were sown in an upland nursery to assess leaf blast in early July. In order to inoculate the desired lines, leaves infected with the blast were collected from different areas of Guilan Province and placed on the surface of the rice blast nursery. In all stages, the necessary humidity to cause disease was provided by sprinkler irrigation. The severity of the disease was rated from zero to nine using the standard method of the International Rice Research Institute. Seedlings were grown in large pots in the greenhouse and inoculated by injection into the panicle neck to assess panicle burst. Results: Seven and 14 lines with grades 2 and 3 showed resistance to leaf blast. Other lines ranged from relatively susceptible to very susceptible to the disease. Three lines TH1, TH2, and TH3 with a degree of contamination of 5 were susceptible to panicle blast disease. Conclusion: Twenty-one known blast-resistant lines can be used in the rice cultivars breeding program.
Hamid Sadeghi Garmaroodi, Seyed Yaghob Seyed Masoumi, Ashraf Nankali,
Volume 11, Issue 1 (3-2022)
Abstract
Sadeghi Garmaroodi H, Seyed Masoumi SY, Nankali A (2022) The reaction of thirteen peach and nectarine cultivars to Verticillium wilt. Plant Pathology Science 11(1):60-73. Doi: 10.2982/PPS.11.1.60.
Introduction: Verticillium wilt, caused by Verticillium dahliae, is one of the most important soil-borne diseases of stone-fruit trees. Materials and Methods: Samples showing evidence of Verticillium wilt were collected from stone fruit orchards in the suburbs of Shahroud and Damavand and four isolates of V. dahliae were obtained. The fungal inoculum was prepared on sterile wheat grains and the response of 13 peach and nectarine cultivars propagated by grafting on GF305 (almond-peach hybrid) rootstock was inoculated with it in the canopy area in the garden in early spring. Sixteen weeks after inoculation, disease severity was recorded on a three-point scale. Results: All peach and nectarine cultivars were classified as very susceptible, susceptible, or semi-susceptible and none of them showed resistance. The Nectarine Independence cultivar was highly susceptible to disease, while the peach cultivar had the lowest disease severity index and was therefore classified as semi-susceptible. Conclusion: Among these cultivars, the Romestar peach cultivar is less susceptible to the disease.
Maryam Mirtalebi, Javad Shafienia,
Volume 11, Issue 2 (9-2022)
Abstract
Shafienia J, Mirtalebi M (2022) Plants growth promoting fungi. Plant Pathology Science 11(2):112-124. Doi:10.2982/PPS.11.2.112
The world's population is growing rapidly. To feed this large population, more crops must be produced. The broad use of fertilizers, pesticides and other inputs increases the productivity of existing agricultural lands and the amount of food production, but this increase in yield causes damage to the environment, soil degradation and the destruction of its natural microbial population. To prevent these harmful effects of the chemicals, an eco-friendly approach is needed to ensure human health and the environment. In the innovative view of agricultural production, there is a growing demand for the use of biofertilizers instead of agrochemicals. The use of beneficial microorganisms can be a new strategy to improve plant health and productivity. Natural microbial populations play an important role in the solubilizing and mineralizing of soil nutrients, which is very important for plant growth and development. Among the various microbial populations, fungi known as plants growth promoting fungi (PGPF) have recently received increasing attention. For decades, plant growth promoting fungi such as Trichoderma, Penicillium, Phoma, Aspergillus and Fusarium have been studied. Studies have shown that these fungi, regulate plant growth without environmental pollution and increase plant tolerance to plant pathogens. In this review a brief description of plant growth promoting fungi is given first. In the following, the nature and composition of these fungi as well as the mode of actions, formulation and related challenges will be concidered.
Farzad Moradi, Hojatolah Mazaheri-Laghab, Leila Kashi, Seid Saied Mosavi,
Volume 11, Issue 2 (9-2022)
Abstract
Moradi F, Mazaheri-laghab H, Kashi L, Moosavi SS (2022) Impact of raw and pure saponins of six alfalfa ecotypes on Ditylenchus dipsaci egg hatching. Plant Pathology Science 11(2):61-72. Doi: 10.2982/PPS.11.2.61
Introduction: The stem and bulb nematode, Ditylenchus dipsaci, is an important and damaging pathogen in a number of agricultural and ornamental plants, including alfalfa. The aim of this study was to investigate the effect of raw and pure saponins of six alfalfa ecotypes on the hatching of this nematode's eggs in order to find a biological method for its management. Materials and Methods: The effect of raw and pure saponins of six alfalfa ecotypes on the hatching of stem nematode eggs was investigated in a completely randomized factorial design with two factors of alfalfa ecotypes (six ecotypes) and their saponins (raw and pure) in vitro. Results: Analysis of variance showed that the interaction effect between two factors, ecotype and saponin, is statistically significant. At concentrations of 50 and 90 microliters of crude saponin from different ecotypes, 30-42% and 33-59% of the nematode eggs did not hatch, respectively. The Nishaburi ecotypes caused the most and the Shiraz and Khrisari polycross caused the least number of egg hatching. Concentrations of 10 and 50 microliters of pure saponin resulted in between 56 and 69% and 61 and 79% of total nematode eggs failing to hatch, respectively. The local ecotypes Miandoab and Nishaburi had the highest and Shiraz Polycross the lowest number of egg hatches. Conclusion: Pure saponins of alfalfa ecotypes have a greater effect on nematode egg hatching than raw saponins. Saponins of Shiraz Polycross alfalfa ecotype have a better effect than other ecotypes.
Farzaneh Talaei, Mehdi Sadravi, Ebrahim Adhami,
Volume 11, Issue 2 (9-2022)
Abstract
Talaei F, Sadravi M, Adhami E (2022) Correlation between arbuscular mycorrhiza in wheat and physicochemical characteristics of soil. Plant Pathology Science 11(2):32-41. Doi: 10.2982/PPS.11.2.32
Introduction: Arbuscular mycorrhizal fungi (AMF) in symbiosis with plant roots help to absorb more phosphorus, and increase the growth and development of plants. The aim of this study was to determine the correlation between population and diversity of AMF, and physicochemical characteristics of soil in wheat fields of Kohgiluyeh and Boyer-Ahmad province in southwestern Iran. Materials and Methods: Thirty wheat fields in this province, were visited near harvest time, and their rhizosphere and aerial organs were sampled. AMF spores were isolated by sieving the rhizosphere suspension in water and centrifuging in Sucrose solution. The spore population of these fungi was count. The percentage of root length colonization by these fungi was calculated in every sample. The morphological characteristics of the isolated spores were studied and the collected information was compared with the descriptions of AMF and the fungi present in each sample were identified. Texture, soil dispersion, soil and plant phosphorus content were determined and the correlation coefficient between AMF population and diversity with physical and chemical characteristics of soil and wheat plant were calculated. Results: Fifteen arbuscular mycorrhizal fungi belonging to nine genera: Acaulospora, Archaeospora, Entrophospora, Gigaspora, Claroideumglomus, Funeliformis, Rhizoglomus, Septoglomus and Scutellospora were identified. The diversity of these fungi in the samples was (2-)2.9(-5) and F. mosseae was dominant species with 90% relative frequency. The population of these fungi had a negative correlation with soil pH, but with soil soluble phosphorus and plant phosphorus content had a positive correlation. The diversity of these fungi had a positive correlation with soil soluble phosphorus. There was a positive correlation between the amount of sand in the soil and the root length colonization. Conclusion: AMF have a greater population and diversity in soils with light texture, less moisture along with low amounts of soluble phosphorus and organic matter. The positive correlation between the population of these fungi and plant phosphorus indicates their usefulness for the plant.
Amir Hossein Mohamadi, Masoumeh Haghdel, Mansoyreh Mirabolfathy, Hossein Alaei,
Volume 11, Issue 2 (9-2022)
Abstract
Mohammadi AH, Haghdel M, Mirabolfathy M, Alaei H (2022) Armillaria root and crown rot disease in pistachio orchards. Plant Pathology Science 11(2):92-102.
Doi: 10.2982/PPS.11.2.92
Pistachio is one of the most important horticultural products in Iran which its production is always affected by biotic and abiotic limiting factors. Armillaria crown and root rot caused by Armillaria mellea, is an important disease in pistachio orchards, which causes the death of fertile trees. Control of the disease is difficult, costly and rarely successful. Symptoms of the disease in shoots of affected trees includes yellowing, early defoliation, sudden wilting, dieback of branches and reduction shoot growth. These symptoms, together with the decay and browning of woody tissues and the formation of white to creamy mycelial sheets in the roots and crown, as well as the observation of rhizomorphs of the fungus, are sufficient evidence for definitive diagnosis of Armillaria crown and root rot in pistachio trees. The infection process begins with the growth of hyphae or rhizomorphs and their firm attachment to the surface of the roots. The pathogen uses a combination of methods such as mechanical pressure, toxin production, and cell wall-degrading enzymes to penetrate the root tissues. Successful control of the disease is possible only by combining chemical, cultural, biological control methods and use of resistant rootstocks and cultivars due to delay in diagnosis of the disease before the spread of the pathogen in plant and soil and the appearance of symptoms, the presence of an extensive network of rhizomorphs at a distance from the tree and deep in the soil and formation of the mycelium beneath the plant bark or inside dead wood.
Engineer Maryam Fouladi, Eidi Bazgir, Mostafa Darvishnia, Kourosh Azizi,
Volume 11, Issue 2 (9-2022)
Abstract
Fouladi M, Bazgir E, Darvishnia M, Azizi K (2022) Plant parasitic nematodes associated with cucumber cultivation in Lorestan province of Iran. Plant Pathology Science 11(2):73-82.
Introduction: Nematodes are one of the harmful factors for cucumber production in the world. This research was conducted to identify plant parasitic nematodes in cucumber fields and greenhouses in Lorestan province in western Iran. Materials and Methods: Eighty-five soil samples containing cucumber roots were collected from different cities of Lorestan province in western Iran. Extraction of nematodes, their fixation and the production of permanent microscopic slides and species identification were carried out using scientific references. Results: Twenty plant-parasitic nematode species from 12 genera of the order Tylenchina have been identified. The morphological characteristics of Amplimerlinius globigerus and Meloidogyne incognita have been described as the most harmful cucumber nematodes in the province. Conclusion: Eleven new species of cucumber root-associated nematode for Iran are reported here.
Mostafa Nasiri, Mohammad Mehdi Faghihi,
Volume 11, Issue 2 (9-2022)
Abstract
Nasiri M, Faghihi M M (2022) Bacterial mosaic disease of wheat. Plant Pathology Science 11(2):103-111.
Bacterial mosaic of wheat is caused by the gram positive bacterium Clavibacter tessellarius. The symptoms of the disease are uniformly distributed small chlorotic spots with mosaic pattern on the entire leaf surface resemble those symptoms of nutrient deficiencies and some viral diseases, and it may be difficult to determine. Due to the seed-borne nature of the disease, use of healthy and certified pathogen free seeds is the most important strategy for managing this disease. The wheat bacterial mosaic has been reported in wheat fields in several provinces of Iran and it seems to be widespread in different wheat growing areas. In order to better understand the disease, its various aspects including the symptoms, pathogen biology and its host ranges, and management of the disease are reviewed in this article.
Seyed Moslem Hosseini, Mostafa Darvishnia, H R Hossein Rezaeinejad, Eidi Bazgir, Fatemeh Darvishnia,
Volume 11, Issue 2 (9-2022)
Abstract
Hosseini SM, Darvishnia M, Rezaei Nejad A, Bazgir E, Darvishnia F (2022) The effect of essential oils of thyme, peppermint, savory and two fungicides on the growth of three plant pathogenic fungi. Plant Pathology Science 11(2):52-60.
Introduction: Some plant essential oils have antifungal properties. This study was conducted to determine the effect of three plant essential oils and two chemical fungicides on the growth of three plant pathogenic fungi in vitro. Materials and methods: The effect of different concentrations of three essential oils of thyme (Thymus vulgaris L.), peppermint (Mentha piperata L.) and khuzestani savory (Satureja khuzestanica Jamzad), compared to two chemical fungicides; mancozeb and carbendazim were investigated on the growth of three fungi Alternaria solani, Botrytis cinerea and Fusarium solani by dilution in Yeast Extract Sucrose Broth medium method and their minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were calculated. Results: Thyme essential oil had the greatest inhibitory effect on A. solani and F. solani among these essential oils, and savory essential oil had the greatest inhibitory effect on B. cinerea. Among the chemical fungicides, mancozeb had the most inhibitory effect on A. solani and carbendazim had the most inhibitory effect on B. cinerea and F. solani. Conclusion: Thyme and khuzestani savory essential oils have a significant inhibitory effect on the growth of these three plants pathogenic fungi.
Dr. Ali Alizadeh Aliabadi, Dr. Mohammad Mehdi Faghihi, Dr. Mohammad Salehi, Mr. Abolghasem Ghasemi,
Volume 11, Issue 2 (9-2022)
Abstract
Alizadeh Aliabadi A, Faghihi MM, Salehi M, Ghasemi A (2022) Dynamics of emergence and spread of citrus huanglongbing disease in Iran. Plant Pathology Science 11(2):11-21. Doi: 10.2982/PPS.11.2.11
Introduction: Huanglongbing or Fruit Greening is one of the major citrus diseases in Iran that has spread to most of the country's citrus growing provinces. In this study, the prevalence trend of the disease in seven provinces was investigated and analyzed. Materials and Methods: Citrus orchards in Sistan-Balochestan, Hormozgan, Bushehr, Khuzestan, Fars, Kerman and Mazandaran provinces were visited and samples suspected of having this disease were collected, in 2007-2009 and 2017-2019 years. After DNA extraction from the samples, the PCR test was performed using the specific primers OI1/OI2c and A2/J5. Results: It was found that the percentage of infected specimens, infected areas and number of hosts of Candidatus Liberibacter asiaticus, the causative agent of this disease, has increased over this period. At the end of this period, some samples of oranges, tangerines, grapefruits, bitter oranges, Lisbon lemons and Mexican limes were obtained from the provinces: Kerman, Hormozgan, Sistan-Baluchestan, and Fars were infected with the disease. Conclusion: The study of the disease during these years showed that: the infected areas and the host range of the disease have expanded a lot during this period, and the spread of the disease in citrus cultivation areas in the south of the country has been with a gentle slope. The reduction of the disease-carrying psyllium population during these years seems to have been effective in slowing down the spread of the disease.
Khadijeh Abbasi, Saeed Afzalinia,
Volume 11, Issue 2 (9-2022)
Abstract
Abbasi K, Afzalinia S (2022) Introduction of eight fungi isolated from potato golden cyst nematode in Iran. Plant Pathology Science 11(2):22-31.
Introduction: The golden cyst nematode, Globodera rostochiensis, is considered as one of the most damaging potato pathogens in the world. Considering the skin composition of cyst nematodes and the ability of some fungi to produce enzymes that decompose it, this research was conducted to identify the fungi associated with potato cyst nematode. Materials and Methods: Eighty-six fungal isolates infecting potato golden cyst nematode in Hamedan province in western Iran were isolated and purified and identified based on morphological characteristics by valid keys. Results: Eight species of fungi belonging to three genera Alternaria, Clonostachys, Fusarium were isolated and identified from potato golden cyst nematode. The highest frequency was related to different species of Fusarium. Conclusion: The morphological characteristics of these eight fungi have been described and illustrated.
Mohammad Hosein Poor, Ramin Heydari, Ahmad Drakhshan, Somayeh Baadl Chery,
Volume 11, Issue 2 (9-2022)
Abstract
Hoseinpoor M, Heydari R, Drakhshan A, Baadl Chery S (2022) The effect of ferula and rosemary extracts on tomato root knot nematode. Plant Pathology Science 11(2):83-91.
Introduction: Root-knot nematode (Meloidogyne javanica) is considered as one of the limiting factors in the cultivation of greenhouse crops, including tomatoes. This research was conducted to investigate the effect of rosemary and ferula extracts compared to chemical nematicides of rugby and volum to find an environmentally friendly method for disease management. Materials and Methods: The effect of rosemary and ferula extracts with rugby and volum nematicides on tomato root-knot nematode was investigated in a completely randomized design under greenhouse conditions. Seedlings of Mobil variety were inoculated with second instar larvae of M. javanica at the 4-6 leaf stage and the effects of plants extracts and nematicides were investigated. After 60 days, the plants were removed from the pot and their growth indicators and disease severity were measured. Results: The simultaneous use of rosemary and ferula extracts had the greatest effect in reducing pathogenicity indicators such as gall index, number of larvae and number of eggs and increasing plant growth indicators such as fresh and dry weight of root and fresh weight of stem compared to control infected with nematode and nematicides treatments. Conclusion: The good effect of the combination of ferula and rosemary extracts compared to chemical nematicides shows the appropriate potential of its use as an environmentally friendly method for disease management.
Fariba Ghaderi, Seyed Ali Asghar Hashemi,
Volume 11, Issue 2 (9-2022)
Abstract
Ghaderi F, Hasehemi SAA (2022) Phytophthora citricola as the causal agent of persimmon root rot in Fars province of Iran. Plant Pathology Science 11(2): 1-10.
Introduction: Phytophthora species are a serious threat to plant products worldwide. Therefore, identifying them is the first step in finding a way to treat the disease. The aim of this study was to identify Phytophthora species causing root and crown rot of persimmon trees in Fars province. Materials and methods: Samples were taken from the crowns and roots of diseased persimmon trees, in the summer of 2018-2019. Infected root and crown tissues were cultured in CMA-PARPH medium. Isolates of Phytophthora species were purified by single spore method and morphological and molecular characteristics were used to identify them. Results: Six isolates were obtained from the roots of diseased persimmon trees and identified as Phytophthora citricola based on their morphological characteristics. Phylogenetic studies based on beta-tubulin (βtub) and 28S rDNA genes showed that all isolates (Iran-Pc1 to Iran-Pc6) were grouped into clade 2 with a validation scale of 100 and confirmed the identification of P .citricola. Conclusion: This is a new report of persimmon root and crown rot caused by Phytophthora citricola in Fars Province.
Keywords: Beta-tubulin, Gene, Persimmon, Phytophthora, 28S rDNA
Masoumeh Vakili-Ghartavol, Hossein Arouiee, Dr. Shiva Golmohammadzadeh, Mahboobeh Naseri,
Volume 11, Issue 2 (9-2022)
Abstract
Vakili-Ghartavol M, Arouiee H, Golmohammadzadeh S, Naseri M (2022) Optimal methods for the preparation of fungal mycelium for examination with the scanning electron microscope. Plant Pathology Science 11(2):42-51.
Doi: 10.2982/PPS.11.2.42
Introduction: Plant-pathogenic fungi are considered a serious threat to world food security, causing spoilage in plant products and food poisoning. One of the ways to identify fungi is to examine their mycelium using a scanning electron microscope (SEM). In order to take high-resolution microscopic images and obtain the surface properties of mushroom mycelium samples, it is very important to prepare the samples, i.e. the method of dehydrating and drying them. Materials and Methods: In this research, the effect of two dehydration methods and two drying methods on the mycelia of two fungi, Rhizoctonia solani and Rhizopus stolonifer, were compared to study them with SEM. Results: Microscopic observations showed that for R. stolonifer, dehydration with 100% ethanol and drying and for R. solani at room temperature and dehydration with 50% ethanol and the freezing method produce a smooth and high-resolution image compared to other methods. Conclusion: Dehydration of fungal mycelium with 50% to 100% ethanol and drying at room temperature or freezing are the optimal methods to prepare them for examination with SEM and produce a smooth and high-resolution image, and the surface features of their mycelium are preserved and well-defined.
Mr Noorallah Hasanpour, Mahdi Arzanlou,
Volume 12, Issue 1 (3-2023)
Abstract
Hasanpour, N., & Arzanlou M. (2023). Application of yeasts in plant diseases management. Plant Pathology Science 12(1), 94-104.
Yeasts are widely distributed on earth.They play role in industries such as bakery, fermentation, protein production, the synthesis of chemicals such as riboflavin and citric acid, agriculture and plant diseases management. They are effective in reducing plant diseases. through a variety of mechanisms, such as competition for food and space, parasitic relationship with the pathogen, induction of plant resistance, mycoparasitism, ecological niche occupation, mycotoxin detoxification and production of cell wall degradation enzymes. The superior characteristics of yeasts are greater tolerance and adaptation to thermal fluctuations at low and high temperatures, adaptation in a wide range of relative humidity, pH fluctuations, low oxygen levels, high proliferation rate, limiting the pathogen population and tolerance to UV radiation. Due these characteristics, yeasts are considered as one of the best choices to use in the process of biological control. In this paper, the positive aspects of yeasts compared to other microbial agents, their role in agriculture and management mechanisms of plant diseases are discussed.
Azadeh Goudarzi,
Volume 12, Issue 1 (3-2023)
Abstract
Goudarzi, A. (2023). Fusarium wilt disease of date palm. Plant Pathology Science, 12(1), 64-73.
Abstract
Fusarium wilt disease is known as one of the most destructive and fatal diseases of date trees in many date-producing areas of the world, especially date-producing countries in Africa. Currently, a significant portion of date orchards in Morocco and the western and central Sahara of Algeria are affected by Fusarium wilt disease. The economic losses caused by Fusarium wilt disease of dates, especially in the incidence of severe epidemics, are significant. Over the past century, the disease has killed more than 12 million palm trees in Morocco and about three million palm trees in Algeria. Fusarium oxysporum (Schltdl.) f. sp. albedinis, a soil-borne vascular pathogen, is known to cause Fusarium wilt disease of dates. The economic losses caused by this disease are very significant, especially in the incidence of severe epidemics, and in some areas, the Fusarium wilt epidemic has destroyed more than 70% of the date fruits. Integrated disease management strategies include the use of resistant or tolerant cultivars, prevention of introduction of the disease causal agent into disease-free areas, eradication of the disease, biological control and chemical control. This disease has not been reported from date-producing areas of Iran, although similar symptoms to Fusarium wilt have been observed in some date orchards of Hormozgan province.
Azadeh Habibi, Fariba Ghaderi, Ziaeddin Banihashemi,
Volume 12, Issue 1 (3-2023)
Abstract
Habibi A, Ghaderi F, Banihashemi Z (2023) Coevolution of Polystigma amygdalinum through a process of host tracking. Plant Pathology Science 12(1):36-45.
Introduction: The almond tree (Prunus dulcis) and its wild relative, the mountain almond tree (Amygdalus scoparia), grew up together in the province of Fars in Iran over decades. Red leaf blotch disease caused by Polystigma amygdalinum is one of the most important almond diseases in the world. This research was conducted with the aim of investigating the evolution of this pathogen on its wild and domestic hosts. Materials and Methods: Ascospores suspension of P. amygdalinum isolates obtained from almond in Fars province was inoculated to almond and mountain almond seedlings in a greenhouse. The progress of the disease in the diseased leaves of mountain almond compared to almond was investigated by sectioning with a freezing microtome from the spots created on the leaves. Results: Red leaf blotch spots appeared on the leaves of both types of almonds. Statistical analysis of test data showed that P. amygdalinum isolates from almond are able to cause disease in mountain almond with significantly lower severity, and longer incubation period. Conclusion: The results of this research show that P. amygdalinum, the cause of red leaf blotch disease, have coevolved on cultivated almond through a process of host tracking.
Elmira Abootorabi,
Volume 12, Issue 1 (3-2023)
Abstract
Abootorabi E (2023) The reaction of 32 tomato genotypes to root-knot nematode Meloidogyne javanica. Plant Pathology Science, 12(1):25-35.
Introduction: This research was conducted with the aim of determining the reaction of 32 tomato genotypes from the plant gene bank of Iran to the root-knot nematode Meloidogyne javanica, to identify resistant genotypes during two years under greenhouse conditions. Materials and Methods: After purifying the nematode population and planting the seeds of 32 tomato genotypes and reaching the stage of 3-4 leaves, each treatment was inoculated with 6000 eggs and larvae of M. javanica in five replicates, and 60 days after inoculation, vegetative traits and traits related to nematode population were determined in each treatment, and based on gall index (GI) and nematode reproductive factor (Rf), the reaction of genotypes to nematode was determined. Results: Eighteen genotypes with Rf>1 and GI>2, 23 genotypes with Rf<1 and GI>2, and three genotypes TN-72-938, TN-72-1041 and TN-72-1012 with Rf≤1 and GI≤2, were identified as susceptible, moderately resistant, and resistant to this destructive nematode respectively. Conclusion: These three resistant genotypes can be used in the M. javanica management program.
