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Showing 4 results for MIRZAEI
Role of Soil Solarization in Management of Soil-Borne Diseases
Zabihollah Azami-Sardooei , Abdolrahman Mirzaei , Farnaz Fekrat ,
Volume 6, Issue 1 (2-2017)
Abstract

Nowadays, control of plant pathogens and weeds is more difficult and expensive than in the past. In last decades, due to adoption of inappropriate management methods and excessive use of agrochemicals, the soil fauna and flora threatened. Accordingly, many of ecologists and plant pathologists tried to find some alternative methods of pest and pathogen control. Soil solarization is of these approaches that is widely used against soil pathogens. This is an ecofriendly and safe as well as low cost and efficient method which can be used to control the plant pathogens, pests and weeds. As a part of integrated pest management program, this approach applies the ecological principles to protect the environment and reduce the hazards of pesticides. In this review, we have described the history and benefits of soil solarization and also the principles of this method


Pathogenic variation of Didymella rabiei isolates causing Chickpea blight in three western provinces of Iran
Soraya Mirzapour, Mostafa Darvishnia, Eidi Bazgir, Hossein Mirzaei Najafgholi,
Volume 9, Issue 2 ((Spring and Summer) 2020)
Abstract
Mirzapour S, Darvishnia M, Bazgir E, Mirzaei Najafgholi H (2020) Pathogenic variation of Didymella rabiei isolates causing Chickpea blight in three western provinces of Iran. Plant Pathology Science 9(2):1-13. DOI: 10.2982/PPS.9.2.1
 
Introduction: Chickpea blight, caused by Didymella rabiei, is the most limiting factor in chickpea production areas in the world, including the western provinces of Iran. The aim of this study was to investigate the pathogenic diversity of the isolates collected from western provinces of Iran (Ilam, Lorestan and Kermanshah) on differential lines of chickpea, as well as the relationship between pathogenic diversity of the isolates and their geographical origins. Materials and Methods: During the 2017-18 chickpea growing season, samples of infected plants were collected from fields in western provinces and transferred to the laboratory. Then 100 pure isolates of D. rabiei were obtained. Based on the location of collection, the isolates were divided into 20 groups. Based on the morphological characteristics, one isolate from each group was selected as a representative for greenhouse experiments. The pathogenic diversity of theses 20 isolates was assessed on eight differential lines of chickpea. The factorial experiments were performed in a completely randomized block design under greenhouse conditions. Results: The results of analysis of variance showed that there was a significant difference between differential lines and isolates at a probability level of p<0.01. Based on the response of the differential lines, the pathogenic isolates were grouped into three pathogenic groups, including pathogenic group 1 (weakly aggressive), group 2 (aggressive or moderately aggressive), and group 3 (highly aggressive). The isolates from Kermanshah and Lorestan Province were classified into Group 1 and the Ilam isolates into Groups 1, 2 and 3. The greatest severity of the disease was found in three isolates of Ilam province. The variety ILC3996 showed the highest resistance to all isolates. Conclusion: D. rabiei isolates from three western provinces of Iran are divided into three pathogenic groups: 1 (low aggressive), 2 (moderately aggressive), and 3 (highly aggressive). Highly aggressive isolates identified in Ilam province can be used in chickpea breeding programs to produce resistant cultivars to the disease.. The wild variety ILC3996 has resistant genes against these pathogenic groups and showed resistance to all of these isolates.

Effect of temperature and culture medium on the growth and sporulation of eight Trichoderma species
Zahra Mirzaeipour, Eidi Bazgir, Doustmorad Zafari, Mostafa Darvishnia,
Volume 12, Issue 2 ((Spring and Summer) 2023)
Abstract
Mirzaeipour Z, Bazgir E, Zafari D, Darvishnia M (2023) Effect of temperature and culture medium on the growth and sporulation of eight Trichoderma species. Plant Pathology Science 12(2):105-116. DOI: https://doi.org/10.2982/PPS.12.2.105

Trichoderma species are important agents of biological control of soil-borne plant pathogens. The growth and reproduction of these fungi are influenced by the culture medium and temperature. This study was conducted to determine the effect of temperature and culture medium on the growth and sporulation of Trichoderma species. Ten isolates of Trichoderma species were isolated from agricultural soils of different regions of Lorestan Province, Iran. The study of morphological characteristics and sequencing of ITS-rDNA, and tef1α gene regions showed that they are belong to eight species of Trichoderma. Investigating the effect of four types of culture medium and five temperatures to determine optimum culture medium and temperature for the growth and reproduction of these fungi, showed that the Potato/Dextrose/Agar (PDA) medium is the best, and the temperature of 20 to 30 degrees Celsius is optimal for the growth and reproduction of these fungi. Evaluation of their ability to inhibit the growth of the soil-borne plant pathogenic fungus Rhizoctonia solani in vitro, showed that T. harzianum LT8 has the most inhibition ability. Therefore, this isolate can be used as a potential biocontrol agent for this plant pathogenic fungus in future research.
 

Morphological and molecular characteristics of two species of Psilnchus (Nematoda: Psilenchidae) from the Taf region of Lorestan province, Iran
Dr. Arezoo Naghavi, Dr. Farahnaz Jahanshahi Afshar, Dr. Eidi Bazgir, Dr. Zahra Mirzaeipour,
Volume 13, Issue 2 ((Spring and Summer) 2024)
Abstract
The species of the genus Psilenchus are weak parasites of plants and mosses that feed on hairy roots and epidermal cells of root. Previously, the members of the genus were placed in the family Tylenchidae because of having weak stylet and elongate tail, but because of the didelphys uterus and the presence of phasmid on the tail, they were more similar to the members of the supper family Dolichodoroidea, so “Psilenchidae”, as a new family with two subfamilies Antarctenchinae (Stylet knobbed and vulva with lateral membranes and epiptygma) and Psilenchinae (Stylet not knobbed and vulva without lateral membranes and epiptygma) was established. The genus Psilenchus currently has 21 valid species (only four species have been sequenced), of which 10 species have been recorded from Iran. About 100 samples were collected from the rhizosphere of fruit and forest trees in Taf region of Khoramabad county in 2022 and 2023. The nematodes were extracted using tray method and after fixation, permanent slides were prepared. The morphological and morphometric characteristics of the samples were examined, then a molecular study was performed using the D2-D3 28S rDNA marker. Two species, Psilenchus curcumerus and P. vinciguerrae, were identified with one and two sequences, respectively. This is the first report of these two species from the region. These two species were confirmed based on morphometric and morphological characteristics and phylogeny studies.

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