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Showing 4 results for Jahanshahi Afshar

Former Ms.c. Saeed Sharify Rostam-Abady, Dr. Mahdieh Rostami, Dr. Farahnaz Jahanshahi Afshar, Fariba Ardeshir,
Volume 12, Issue 1 ((Autumn & Winter) 2023)
Abstract

Sharify Rostam-Abadi, S., Rostami, M., Jahanshahi Afshar, F., Ardeshir, F. (2023).  Three plant-parasitic nematodes of the family Longidoridae from orchards of Rafsanjan county, Iran. Plant Pathology Science 12(1),12-24.  
 Introduction: Longidoridae is a family of plant-parasitic nematodes belonging to the order Dorylaimida, that cause damage to most agricultural crops especially fruit trees by direct damage and also transmitting some plant pathogenic viruses. Considering the lack of prior research studies on this topic in the fruit orchards of Rafsanjan region, identifying and monitoring their population is important. Materials and Methods: In a study that was conducted to identify plant-parasitic nematodes of horticultural products in Rafsanjan city, some soil samples were collected from the rhizosphere of various fruit trees, in 2020. The nematodes were extracted from the soil samples using two methods, centrifugal flotation-sieving and tray technique, fixed and transferred to the anhydrous glycerin. After preparing permanent slides, the nematodes were studied using a light microscopy. The nematode species were identified based upon morphological and morphometric data using relevant valid references. Results: In this study, three species of Dorylaimida order belonging to the Longidoridae family including the two genera: Longidorus (L. africanus) and Xiphinema (X. vuittenezi and X. index) were obtained from the rhizosphere. Also, in order to confirm the traditional identification of X. vuittenezi, this species was molecularly studied using D2-D3 extension fragment of LSU rDNA. The obtained sequence of the species was 100% identical to the sequences of the other populations of X. vuittenezi in the GenBank. Conclusion: This study shows that regional nematode population monitoring needs more attention.



 
Former Ms.c. Saeed Sharify Rostam-Abady, Mahdieh Rostami, Farahnaz Jahanshahi Afshar, ,
Volume 12, Issue 2 ((Spring and Summer) 2023)
Abstract

Sharify Rostam-Abadi S, Rostami M,  Jahanshahi Afshar F, Ardeshir F (2023) Report of six plant parasitic nematodes from the orchards of Rafsanjan region, Kerman Province, Iran. Plant Pathology Science 12(2):41-52.
Introduction: In the suburban area of Rafsanjan city in Kerman Province, Iran, there are many orchards infested with plant parasitic nematodes. Therefore, it was necessary to identify the plant parasitic nematodes in the orchards of this region. Materials and Methods: In 2018 and 2019, some soil samples were taken around the roots of apricot, peach, pomegranate, apple, cherry and persimmon trees in this area. Nematodes were extracted by two methods, tray and sieve-centrifuge, and after fixation, they were transferred to pure glycerin. Permanent slides were examined with a bright field microscope and extracted nematodes were identified using diagnostic keys and original descriptions. Results: Seven nematodes from the infraorder Tylenchomorpha, belonging to five families including: Tylenchidae, Pratylenchidae, Tylenchulidae, Criconematidae and Anguinidae were identified. Root-lesion nematode (Pratylenchus penetrans), pin-nematode (Paratylenchus neoamblycephalus) and Criconematid (Mesocriconema xenoplax) were the most important plant parasitic nematodes in fruit orchards of this region. Conclusion: Due to the presence of some important and damaging nematodes in the orchards of Rafsanjan, it is recommended to implement appropriate management methods and monitor the population of important plant parasitic nematodes.


 
Student Sarina Zaker Abbasali, Dr Navazollah Sahebani, Dr Farahnaz Jahanshahi Afshar, Dr Soheil Karimi,
Volume 13, Issue 2 ((Spring and Summer) 2024)
Abstract

The Mazandaran Province occupies more than half of the area of Hyrcanian forests in the north of Iran and has a very diverse vegetation. In order to be aware of the entry of important plant parasite nematodes into these forests and to prevent their spread to downstream farms and gardens, continuous monitoring and determination of the density of important plant parasite nematodes in this area was necessary. Ninety rhizosphere samples of forest trees, including maple, birch, oak, raspberry, alder, walnut, and hornbeam, were collected from this province in 2020.  Nematodes were extracted from the samples by wet sieving and centrifugation method, and after fixing, permanent slides were prepared from them and their morphological indices were measured and identified using valid keys. Also their frequency in the samples and their population density in 200 cm3 rhizosphere were determined. Nine nematode species from the superfamily Criconematoidea were identified from the rhizosphere of these trees under the following names: Criconemoides informis, Criconemoides parvus, Mesocriconema solivagum, Mesocriconema xenoplax, Crossonema civellae, Crossonema menzeli, Ogma fagini, Xenocriconemella macrodora, and Paratylenchus straeleni. Among these nematodes, C. parvus had the highest frequency, and population density in the rhizosphere of alder trees in the Dohaz forest of this province. Some of these plant parasitic nematodes in high populations, in addition to direct damage to the roots, cause the spread of some plant diseases and exacerbate the effects of frostbite.
 
Dr. Arezoo Naghavi, Dr. Farahnaz Jahanshahi Afshar, Dr. Eidi Bazgir, Dr. Zahra Mirzaeipour,
Volume 13, Issue 2 ((Spring and Summer) 2024)
Abstract

The species of the genus Psilenchus are weak parasites of plants and mosses that feed on hairy roots and epidermal cells of root. Previously, the members of the genus were placed in the family Tylenchidae because of having weak stylet and elongate tail, but because of the didelphys uterus and the presence of phasmid on the tail, they were more similar to the members of the supper family Dolichodoroidea, so “Psilenchidae”, as a new family with two subfamilies Antarctenchinae (Stylet knobbed and vulva with lateral membranes and epiptygma) and Psilenchinae (Stylet not knobbed and vulva without lateral membranes and epiptygma) was established. The genus Psilenchus currently has 21 valid species (only four species have been sequenced), of which 10 species have been recorded from Iran. About 100 samples were collected from the rhizosphere of fruit and forest trees in Taf region of Khoramabad county in 2022 and 2023. The nematodes were extracted using tray method and after fixation, permanent slides were prepared. The morphological and morphometric characteristics of the samples were examined, then a molecular study was performed using the D2-D3 28S rDNA marker. Two species, Psilenchus curcumerus and P. vinciguerrae, were identified with one and two sequences, respectively. This is the first report of these two species from the region. These two species were confirmed based on morphometric and morphological characteristics and phylogeny studies.


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