Volume 2, Issue 1 (3-2013)
Plant Pathol. Sci. 2013, 2(1): 25-33 |
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SAFAIE FARAHANI B, MOSTOWFIZADEH-GHALAMFARSA R. (2013). DNA Microarrays Technology and its Applications in Plant Pathology . Plant Pathol. Sci.. 2(1), 25-33.
URL: http://yujs.yu.ac.ir/pps/article-1-47-en.html
URL: http://yujs.yu.ac.ir/pps/article-1-47-en.html
of Plant Pathology, Department of Plant Protection, Collage of Agriculture, Shiraz University, Shiraz, Iran. , rmostofi@shirazu.ac.ir
Abstract: (18911 Views)
DNA microarrays technology is a method for studying the gene expression in large scale, based on investigations of probes and targets hybridization. This technology can also be used for identification of different organisms. DNA microarrays are a set of probes linked to a solid phase as microscopic spots. After hybridization of targets to probes, hybridization level is calculated by means of different methods such as measuring refulgence of fluorescent dyes to determine gene expression level. A microarray examination has different steps: making DNA chips, preparing targets, performing hybridization, and gathering and analyzing data. DNA microarrays technology can be used in different fields of plant pathology such as identification of different species of fungi, bacteria, nematodes and viruses, and to study plant-pathogen interaction.
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