Plant Pathology Science
Volume 14, Issue 2 ((Spring and Summer) 2025)
Plant Pathol. Sci. 2025, 14(2): 76-88 |
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Darvishnia M, Mirderikvand M, Pakbaz S. (2025). Fungi associated with rapeseed roots and crown in Lorestan Province, Iran. Plant Pathol. Sci.. 14(2), : 7
URL: http://yujs.yu.ac.ir/pps/article-1-501-en.html
URL: http://yujs.yu.ac.ir/pps/article-1-501-en.html
Lorestan University , Darvishnia.m@lu.ac.ir
Abstract: (59 Views)
Rapeseed is one of the major oilseed crops worldwide. Owing to its high yield potential per unit area, its cultivation has expanded considerably over the past three decades in many countries, including Iran. Several soil-borne fungi can cause root and crown rot of rapeseed. This study was conducted to identify fungi associated with the roots and crowns of diseased rapeseed plants collected from fields in Lorestan Province, western Iran. Sixty-one samples were collected from the roots and crowns of diseased rapeseed plants in Mamulan, Khorramabad, and Selseleh counties of Lorestan Province, Iran, during the 2023–2024 growing season. Fungi were isolated and purified using selective culture media, and their morphological characteristics were examined. Molecular identification was performed by sequencing the internal transcribed spacer (ITS) and translation elongation factor 1-alpha (TEF1-α) genomic regions. Phylogenetic relationships between the obtained isolates and other species within the corresponding genera were subsequently determined. Based on morphological and phylogenetic analyses, four fungal species associated with diseased rapeseed roots and crowns in the study area were identified as Fusarium clavum, Bipolaris coffeana, Alternaria alternata, and Alternaria tenuissima. Fusarium clavum has previously been reported as a causal agent of root and crown rot of rapeseed in Iran. Bipolaris coffeana is reported for the first time from rapeseed in Lorestan Province. The results demonstrate that the integration of morphological characteristics with sequencing of the ITS and TEF1-α genomic regions provides a reliable and accurate approach for identifying species of Alternaria, Fusarium, and Bipolaris. The morphological characteristics of the identified fungi are described, and their phylogenetic relationships with other species within each genus are presented. The findings highlight the importance of continuous monitoring of soil-borne fungal pathogens in rapeseed-growing fields to facilitate their timely detection and effective disease management.
Article number: 7
Type of Study: Research |
Subject:
Mycology
Received: 2026/06/8 | Accepted: 2026/08/31 | Published: 2026/10/10
Received: 2026/06/8 | Accepted: 2026/08/31 | Published: 2026/10/10
References
1. Alaei, H., Baeyen, S., Maes, M., Hofte, M. & Heungens K. (2009). Molecular detection of Puccinia horiana in Chrysanthemum x morifolium through conventional and real-time PCR. Journal of Microbiological Methods, 76(2), 136-145.
2. Crous, P.W. & Wingfield, M. J. (2021). Fungal Planet Description Personia. Sheets: 1182-1283. Persoonia, 46: 313-528.
3. Choi, Y. W., Hyde, K. D. & Ho, W. H. (1999). Single spore isolation of fungi. Fungal Diversity, 3: 29-38.
4. Dixit, R., Singh, A. & Sharma, P. (2020). Eco-friendly management of Alternaria brassicae in canola using biocontrol agents. Journal of Crop Health, 45(1), 10-18.
5. Darvishnia, M., Alizadeh, A., Mohammadi Goltapeh, E.& Zare, R. (2007). Three new Fusarium taxa isolated from gramineous plants in Iran. Rostaniha. 8(2), 193-206 [In Persian].
6. Farr, D. F. & Rossman, A. Y. (2022). Fungal Databases US. National Fungus Collections. USDA-ARS, Systematic Mycology and Microbiology Laboratory. Available from: https://nt.ars-grin.gov/fungaldatabases/.
7. Heidari, K., Farokhinejad, R. & Mehrabi-Koushki, M. (2018). 'Detection and molecular identification of Colletotricum species causing anthracnose disease of some plants in Khuzestan province (Iran)', Rostaniha 19(1), 19-32 [In Persian].
8. Gautam, A. K. & Avasthi, S. (2019). Methods in Fungal Biology: A Manual of Laboratory Protocols. Scientific Publishers, India. Available online:. https://books.google.com/books/about?id=7zP3DwAAQBAJ.
9. Geiser, D. M., Jiménez-Gasco, M. & Kang, S. (2004). Fusarium -ID v. 1.0: A DNA sequence database for identifying Fusarium. European Journal of Plant Pathology, 110(5-6), 473-479.
10. Geng, X., Mvchir, H., Liu, J., Hua, K., Miao, Q. & Shu, J. (2025). Molecular characterzation and pathogenicity analysis of Alternaria alternate associated with leaf spot Disease of Toona sinensis in china. Horticulturae, 11(279), 1-12.
11. Jin, W. U., Tingting, D. A. I., Xizhuo, W. & Yonglin, W. (2025). Molecular Detection Methods for Forest Pathogens. Critical Reviews Plant Sciences, 44(2), 47-69.
12. Karami, S., Javan-Nikkhah, M., Bardi-Fotuhifar, K. H., Rahjoo, V., Ahmadpour, A. & Alidadi, A. (2020). Study on Bipolaris and Curvularia species associated with corn, sorghum and sugarcanein Iran. Iranian Journal of Plant Protection Science, 51(1), 129-146 [In Persian].
13. Kumar, V., Jat, H. S., Sharma, P. C., Singh, B., Gathala, M. K., Malik, R. K., Kamboj, R. B., Yadav. A. K., Ladha, J. K., Raman, A., Sharma, D. K. & MaDonald, A. (2018). Can productivity and profitability be enhanced in intensively managed cereal systems while reducing the environmental footprint of production? Assessing sustainable intensification options in the breadbasket of India. Agriculture, Ecosystems and Environment, 252, 132-147.
14. Leslie, J. F. & Summerell, B. A. (2006). The Fusarium laboratory manual. Blackwell Publishing, Ames. Sydowia, 48(1), 32-43.
15. Manamgoda, D., Rossman, A. Y., Castlebury, L. A., Crous, P. W., Madrid, H., Chukeatirote, E. & Hyde, K. D. (2014). The genus Bipolaris. Studies in Mycology, 79(1), 221-288.
16. May, S. R. (2022). Tips for the purification of fungi and oomycetes. National Plant Diagnostic Network Newsletter. Available online: https://www.npdn.org/newsletter/2022/01/article/3.
17. Mirdrikvand, M., Darvishnia, M., Bazgir, E. & Pakbaz, S. (2021) Introduction of Fusarium species associated with crown and root of canola in Lorestan Province of Iran. Plant Patholgy Science, 10(1), 64-75 [In Persian].
18. Nelson, P. E., Toussoun, T. A. & Marasas, W. F. O. (1983). Fusarium species: An illustrated manual for identification. Pennsylvania State University Press, University Park.
19. Ne'mati Mandani Pour, O., Farrokhinejad, R. & Mehrabi-Koushki, M. (2021). Identification of Fusarium species associated with root rot symptoms of rapeseed in Khuzestan Province. Journal Applied Research Plant Protection, 10(1), 29-43.
20. O'Donnell, K., Kistler, H. C., Cigelnik, E. & Ploetz, R. C. (1993) Multiple evolutionary origins of the fungus causing Panama disease of banana. Proceedings of the National Academy of Sciences, 90(7), 3127-3131.
21. Sharafi, Z. & Sadravi, M. (2015). Introduction to ten seed-borne pathogenic fungi. Plant Pathology Science, 4 (1), 34-45. (In Persian).
22. Simmons, E. G. (2007). Alternaria: An identification manual. CBS Biodiversity Series 6, Utrecht.
23. Tamura, K., Stecher, G. & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729.
24. Torabi, B., Habibpour-Kashfi, E., Soltani, A. & Zeynali, E. (2025). Evaluation of adaptation strategies of rapeseed (Brassica napus L.) to future climate in Iran. Journal of Plant Production Research,
25. Tsuge, T., Harimoto, Y. & Akimitsu K. (2016). Conditional dispensable chromosomes in Alternaria alternata. Current Genetics, 62(2), 123-132.
26. Wang, Y., Liu, X. & Chen, Z. (2022). Taxonomic revision of Fusarium species in China. Mycological Progress, 21(4), 455-470.
27. Xia, J. W., Lombard, L., Sandoval-Denis, M., Zhang, X. G. & Crous, P. W. (2019). Fusarium incarnatum-equiseti species complex: phylogeny, morphology and pathogenicity. Mycologia 111(6), 1069-108.
28. Yu, H., Chang, K. F., Fredua-Agyeman, R., Hwang, S. F. & Strelkov, S. E. (2024). Diversity and pathogenicity of Fusarium root rot fungi from canola (Brassica napus L.) in Alberta, Canada. International Journal of Molecular Sciences, 25(11), 1-20
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