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Volume 14, Issue 2 ((Spring and Summer) 2025)                   Plant Pathol. Sci. 2025, 14(2): 1-13 | Back to browse issues page

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Dehghani F, Chadegani H, Abdollahi M, Rezaei R. (2025). Effect of DL-β-amino-n-butyric acid on okra plants infected with different initial populations of Meloidogyne javanica. Plant Pathol. Sci.. 14(2), : 1
URL: http://yujs.yu.ac.ir/pps/article-1-468-en.html
Department of Plant Protection, College of Agriculture, Yasouj University, Yasouj, Iran , H.charehgani@yu.ac.ir
Abstract:   (135 Views)
DL-β-amino-n-butyric acid (BABA) induces resistance in plants against root-knot nematodes (Meloidogyne spp.) by activating natural defense mechanisms. This study evaluated the effect of BABA on the resistance of okra (Abelmoschus esculentus cv. Clemson Spineless) to M. javanica under greenhouse conditions. Seeds were sown in 1 kg plastic pots and maintained at 27 ± 3℃ under a 16:8 hours of light-dark cycle and with daily irrigation. At the four-leaf stage, seedlings were foliar-sprayed with BABA at concentrations of 0 (control), 0.5, 1, and 2 mM. After 24 hours, plants were inoculated with M. javanica at initial population densities of 0 (control), 1, 2, 4, and 8 second-stage juveniles (J2s). Sixty days post-inoculation, plant growth and nematode reproduction indices were assessed. Results showed that higher BABA concentrations significantly improved plants growth and reduced nematodes populations. Plants treated with 2 mM of BABA and inoculated with 8, 4, 2 and 1 J2s showed increases in shoot length by 43.9%, 45.1%, 32.5%, and 32.2%, respectively; shoot fresh weight by 37%, 38.2%, 69%, and 61.5%; and shoot dry weight by 23.5%, 24.5%, 37.4% and 39.7% compared to untreated control. Meanwhile, the nematode reproduction factor decreased by 25.1%, 25.9%, 14.1% and 38.4%, respectively. This study demonstrated that BABA application improves okra growth against M. javanica. Although BABA had no significant effect on the reproduction factor at the highest nematode population level, all concentrations improved plant growth, even under severe nematode pressure. Foliar application of 2 mM BABA effectively enhances okra resistance to M. javanica under greenhouse conditions. 
Article number: 1
Full-Text [PDF 961 kb]   (100 Downloads)    
Type of Study: Research | Subject: Nematology
Received: 2025/04/21 | Accepted: 2025/07/16 | Published: 2025/09/16

References
1. Abootorabi, E. (2023). The reaction of 32 tomato genotypes to root-knot nematode Meloidogyne javanica. Plant Pathology Science 12(1), 25-35.[In Persian] 2. Alikarami, M., Charehgani, H., & Abdollahi, M. (2017). Nematicidal activity of some plant extracts on root-knot nematode on tomato (Solanum lycopersicum) in vitro and in vivo conditions. Iranian Journal of Plant Protection Science 48(2), 317-326.[In Persian] 3. Andras, C. D., Simándi, B., Örsi, F., Lambrou, C., Missopolinou‑Tatala, D., Panayiotou, C., Domokos, J., & Doleschall, F. (2005). Supercritical carbon dioxide extraction of okra (Hibiscus esculentus L.) seeds. Journal of the Science of Food and Agriculture, 85(8), 1415–1419. 4. Ansari, S., Charehgani, H., & Ghaderi, R. (2019). Resistance of ten common medicinal plants to the root-knot nematode Meloidogyne javanica. Hellenic Plant Protection Journal, 12, 6–11. 5. Apel, K., & Hirt, H. (2004). Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 373–379. 6. Becker, J. C., Bormann, C., Dummer, R., Schwinn, A., & Burg, G. (1994). Specific immune response against melanoma: Analysis at a clonal level. In W. C. Lambert, B. Giannotti, & W. A. Van Vloten (Eds.), Basic Mechanisms of Physiologic and Aberrant Lymphoproliferation in the Skin (pp. 459–467). Springer-Verlag. 7. Berkelaar, E., & Johns, J. (2002). The effect of Moringa leaf spray on the yield of vegetable crops development potential for moringa products. In International workshop, 29th October – 2nd November (p. 516). ASIAFCO, Montpellier: CIRAD. 8. Bestwick, C. S., Brown, I. R., Bennett, M. H., & Mansfield, J. W. (1997). Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv. phaseolicola. Plant Cell, 9, 209–221. 9. Buonaurio, R., Iriti, M., & Romanazzi, G. (2009). Induced resistance to plant diseases caused by Oomycetes and fungi. Petria, 19(3), 130–148. 10. Charegani, H., Majzoob, S., Hamzehzarghani, H., & Karegar-Bide, A. (2012). Effect of various initial population densities of two species of Meloidogyne on growth of tomato and cucumber in greenhouse. Nematologia Mediterranea, 40, 129–134. 11. Charehgani, H., Karegar, A., Djavaheri, M., & Niazi, A. (2022). Systemic induced resistance to the root-knot nematode in tomato by chemical inducers. Journal of Agricultural Science and Technology, 24(1), 71–82. 12. Charehgani, H., Kargarbideh, A., & Djavaheri, M. (2014). Comparison of DL‑β‑amino‑n‑butyric, salicylic and abscisic acid in induction of resistance in tomato infected by Meloidogyne javanica. Iranian Journal of Plant Pathology, 50, 349–358.[In Persian] 13. Charehgani, H. (2020). Effect of wood vinegar, humic acid and Effective Microorganisms against Meloidogyne javanica on tomato. Plant Pathology Science, 9(2), 73–84.[In Persian] 14. Chen, X. Y., & Kim, J. Y. (2009). Callose synthesis in higher plants. Plant Signaling and Behavior, 4, 489–492. 15. Chinnasri, B., Sipes, B. S., & Schmitt, D. P. (2006). Effects of inducers of systemic acquired resistance on reproduction of Meloidogyne javanica and Rotylenchulus reniformis in pineapple. Journal of Nematology, 38(3), 319–325. 16. Cohen, Y., Rubin, A. E., & Vaknin, M. (2011). Post‑infection application of DL‑3‑amino‑butyric acid (BABA) induces multiple forms of resistance against Bremia lactucae in lettuce. European Journal of Plant Pathology, 130(1), 13–27. 17. Cohen, Y., Vaknin, M., & Mauch-Mani, B. (2016). BABA induced resistance: Milestones along a 55‑year journey. Phytoparasitica, 44, 513–538. 18. Cohen, Y. (2002). β‑Aminobutyric acid‑induced resistance against plant pathogens. Plant Disease, 86, 448–457. 19. Dehghanian, S. Z., Abdollahi, M., Charehgani, H., & Niazi, A. (2020). Combined application of salicylic acid and Pseudomonas fluorescens CHA0 on the expression of PR1 gene and control of Meloidogyne javanica in tomato. Biological Control, 141, 104134. 20. Derakhshan, N., Charehgani, H., & Rezaei, R. (2026). Effect of beta-aminobutyric acid on eggplant and okra infected with root-knot nematode under field condition. Journal of Agricultural Science and Sustainable Production. 35(4), 159-175. [In Persian] 21. Di Vito, M., Greco, N., & Carella, A. (1986). Effect of Meloidogyne incognita and importance of the inoculum on the yield of eggplant. Journal of Nematology, 18, 487-490. 22. Dubreuil-Maurizi, C., Trouvelot, S., Frettinger, P., Pugin, A., Wendehenne, D., & Poinssot, B. (2010). β-Aminobutyric acid primes an NADPH oxidase-dependent reactive oxygen species production during grapevine-triggered immunity. Molecular Plant-Microbe Interactions, 23, 1012-1021. 23. Fatemy, S., Moslemi, F., & Bernard, F. (2012). Seed treatment and soil drench with DL-β-amino-butyric acid for the suppression of Meloidogyne javanica on tomato. Acta Physiologiae Plantarum, 34, 2311-2317. 24. Geetha, H. M., & Shetty, H. S. (2002). Induction of resistance in pearl millet against downy mildew disease caused by Sclerospora graminicola using benzothiadiazole, calcium chloride and hydrogen peroxide: A comparative evaluation. Crop Protection, 21, 601-610. 25. Gheysen, G., & Jones, J. T. (2006). Molecular aspects of plant nematode interactions. In R. N. Perry & M. Moens (Eds.), Plant nematology (pp. 234-254). CABI Publishing. 26. Hashemi, S., Abdollahi, M., & Charehgani, H. (2017). Inhibitory effect of Quercus brantii L. extract on Meloidogyne javanica, the causal agent of root-knot disease, in tomato plants. Iranian Journal of Medicinal and Aromatic Plants, 33(1), 39-50. 27. Heidari, F., Charehgani, H., Abdollahi, M., & Adhami, E. (2024). The effect of poultry and pigeon manures on pinto bean infection with the root-knot nematode Meloidogyne javanica. Plant Pathology Science, 13(1), 1-13. 28. Hussey, R. S., & Barker, K. R. (1973). A comparison of methods of inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter, 57, 1025-1028. 29. Hussey, R. S., & Janssen, G. J. W. (2002). Root-knot nematodes: Meloidogyne species. In J. L. Starr, R. Cook, & J. Bridge (Eds.), Plant resistance to parasitic nematodes (pp. 69-77). CAB International. 30. Hussey, R. S., Mims, C. W., & Sobczak, M. (1992). Changes in the structure of Arabidopsis thaliana during female development of the plant parasitic nematode Heterodera schachtii. Protoplasma, 167, 55-65. 31. Hwang, B. K., Sunwoo, J. Y., Kim, Y. J., & Kim, B. S. (1997). Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici. Physiological and Molecular Plant Pathology, 51(5), 305-322. 32. Jacobs, A. K., Lipka, V., Burton, R. A., Panstruga, R., Strizhov, N., Schulze-Lefert, P., & Fincher, G. B. (2003). An Arabidopsis callose synthase, GSL5, is required for wound and papillary callose formation. Plant Cell, 15, 2503-2513. 33. Jahanbazian, L., Mahdikhani-Moghadam, E., Charehgani, H., & Afsharifar, A. (2024). Comparative analysis of antioxidant enzyme activity and defence responses in root-knot nematode resistant and susceptible carrot cultivars. Plant Pathology, 73, 1413-1424. 34. Ji, H., Kyndt, T., He, W., Vanholme, B., & Gheysen, G. (2015). β-Aminobutyric acid-induced resistance against root-knot nematodes in rice is based on increased basal defense. Molecular Plant-Microbe Interactions, 28, 519-533. 35. Justyna, P. G., & Ewa, K. (2013). Induction of resistance against pathogens by β-aminobutyric acid. Acta Physiologiae Plantarum, 35, 1735-1748. 36. Kesba, H., Abdel-Rahman, A., Sayed, S., & Al-Sayed, A. S. (2021). Screening the nematicidal potential of indigenous medicinal plant extracts against Meloidogyne incognita under lab and greenhouse conditions. Egyptian Journal of Biological Pest Control, 31, Article 81. https://doi.org/10.1186/s41938-021-00429-y 37. Luna, E., Pastor, V., Robert, J., Flors, V., Mauch-Mani, B., & Ton, J. (2011). Callose deposition: A multifaceted plant defense response. Molecular Plant-Microbe Interactions, 24, 183-193. 38. M’Piga, P., Belanger, R. R., Paulitz, T. C., & Benhamou, N. (1997). Increased resistance to Fusarium oxysporum f. sp. radicis-lycopersici in tomato plants treated with the endophytic bacterium Pseudomonas fluorescens strain 63-28. Physiological and Molecular Plant Pathology, 50, 301-320. 39. McSorley, R. (1999). Host suitability of potential cover crops for root-knot nematodes. Journal of Nematology, 31(4), 619-623. 40. Moazezikho, A., Charehgani, H., Abdollahi, M., & Rezaei, R. (2020). The evidence for inhibitory effect of Pseudomonas fluorescens CHA0 and aqueous extracts of Datura stramonium and Myrtus communis on tomato plants infected with Meloidogyne javanica (Tylenchida: Heteroderidae). Egyptian Journal of Biological Pest Control, 30, 15. https://doi.org/10.1186/s41938-020-00217-0 41. Mosahaneh, L., Charehgani, H., Abdollahi, M., & Rezaei, R. (2020). Biological control agents in the management of different initial population densities of Meloidogyne javanica in tomato. Acta Phytopathologica et Entomologica Hungarica, 55(2), 161-170. 42. Mozafaryan, S., Abdollahi, M., & Charehgani, H. (2017). Inhibitory effects of Prangos ferulacea and Satureja hortensis on root knot nematode, Meloidogyne javanica. Iranian Journal of Plant Pathology, 52(4), 445-464. [In Persian] 43. Mozaffarian, S., Charehgani, H., Abdollahi, M., & Rezaei, R. (2019). Effect of some macronutrients on the root-knot nematode, Meloidogyne javanica activities in eggplant (Solanum melongena L.). Journal of Plant Protection, 33(4), 397-408. [In Persian] 44. Nicholson, R. L., & Hammerschmidt, R. (1992). Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology, 30, 369-389. 45. Oka, Y., Cohen, Y., & Spiegel, Y. (1999). Local and systemic induced resistance to the root-knot nematode in tomato by DL-β-amino-n-butyric acid. Phytopathology, 89, 138-1143. 46. Pourkhajeh, F., Charehgani, H., Abdollahi, M., & Sadravi, M. (2019). Biocontrol effect of Trichoderma harzianum isolates on root knot nematode Meloidogyne javanica on greenhouse cucumber. Iranian Journal of Plant Pathology, 55(1), 77-82. 47. Rafiee, F., Charehgani, H., & Abdollahi, M. (2019). Evaluation of Burdock and Mountain almond leaf extracts against Meloidogyne javanica on tomato. Archives of Phytopathology and Plant Protection, 52(11-12), 1035-1047. 48. Rostami, S., Charehgani, H., Abdollahi, M., & Rezaei, R. (2021). Evaluation of nano iron and zinc chelated fertilizers on okra (Abelmoschus esculentus) infected with Meloidogyne javanica. Journal of Crop Protection, 10(3), 493-502. 49. Sahebani, N., Hadavi, N., & Omran Zade, F. (2011). The effects of b-amino-butyric acid on resistance of cucumber against root-knot nematode, Meloidogyne javanica. Acta Physiologica, 33, 443-450. 50. Sahebani, N., & Hadavi, N. (2009). Induction of H₂O₂ and related enzymes in tomato roots infected with root knot nematode (M. javanica) by several chemical and microbial elicitors. Biocontrol Science and Technology, 19(3), 301-313. 51. Shekoohi, S. S., Charehgani, H., Abdollahi, M., & Rajabi, H. R. (2021). Combined effect of β-aminobutyric acid and silver nanoparticles on infected eggplants (Solanum melongena L.) with Meloidogyne javanica. Nematology, 23, 1077-1099. 52. Siegrist, J., Orober, M., & Buchenauer, H. (2000). B-amino butyric acidmediated enhancement of resistance in tobacco to Tobacco Mosaic Virus depends on the accumulation of salicylic acid. Physiological and Molecular Plant Pathology, 56, 95-106. 53. Singh, R. R., Ameye, M., Haesaert, G., Deveux, M., Spanoghe, P., Audenaert, K., Rabasse, J. M., & Kyndt, T. (2023). β-Aminobutyric acid induced phytotoxicity and effectiveness against nematode is stereomer-specific and dose-dependent in tomato. Physiologia Plantarum, 175, Article e13862. https://doi.org/10.1111/ppl.13862 54. Soltani Tale, F., Kashi, L., & Zafari, D. (2024). The effect of three Trichoderma species and organic sulfur on root-knot nematode of cucumber. Plant Pathology Science, 13(2), 49-57. [In Persian] 55. Taylor, A. L., & Sasser, J. N. (1978). Biology, identification, and control of root-knot nematodes (Meloidogyne species). Raleigh, North Carolina. 56. Taylor, P., & Netscher, C. (1974). An improved technique for preparing perineal patterns of Meloidogyne spp. Nematologica, 20, 268-269. 57. Ueki, S., & Citovsky, V. (2002). The systemic movement of a tobamovirus is inhibited by a cadmium-ion-induced glycine-rich protein. Nature Cell Biology, 4, 478-485. 58. Vahabi, S., Charehgani, H., Abdollahi, M., & Rezaei, R. (2021). Response of eight melon cultivars to Meloidogyne javanica. Plant Pathology Science, 10(2), 65-73. [In Persian] 59. Zimmerli, L., Hou, B. H., Tsai, C. H., Jakab, G., Mauch-Mani, B., & Somerville, S. (2008). The xenobiotic beta-aminobutyric acid enhances Arabidopsis thermotolerance. Plant Journal, 53, 144-156. [DOI:10.1016/j.biocontrol.2019.104134]

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