جلد 12، شماره 2 - ( (بهار و تابستان) 1402 )
جلد 12 شماره 2 صفحات 116-105 |
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Mirzaeipour Z, Bazgir E, Zafari D, Darvishnia M. (2023). Effect of temperature and culture medium on the growth and sporulation of eight Trichoderma species. Plant Pathol. Sci.. 12(2), 105-116. doi:10.61186/pps.12.2.105
URL: http://yujs.yu.ac.ir/pps/article-1-410-fa.html
URL: http://yujs.yu.ac.ir/pps/article-1-410-fa.html
میرزایی پور زهرا، بازگیر عیدی، ظفری دوست مراد، درویش نیا مصطفی. تاثیر دما و محیط کشت بر رشد و اسپورزایی هشت گونه Trichoderma دانش بیماری شناسی گیاهی 1402; 12 (2) :116-105 10.61186/pps.12.2.105
گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد ، Bazgir.ei@lu.ac.ir
چکیده: (1048 مشاهده)
میرزایی پور ز، بازگیر ع، ظفری د، درویشنیا م (1402) تاثیر دما و محیط کشت بر رشد و اسپورزایی هشت گونه Trichoderma. دانش بیماری شناسی گیاهی 12(2): 116-105.
گونه های Trichoderma از عوامل مهم مهارزیستی بیمارگرهای خاکزی گیاهان هستند. رشد و تکثیر این قارچ‌ها تحت تأثیر محیط کشت و دما قرار می‌گیرد. این پژوهش به منظور تعیین تأثیر دما و محیط کشت بر رشد و هاگزایی گونه‌های Trichoderma انجام شد. ده جدایه گونه‌های Trichoderma از خاک‌های زراعی مناطق مختلف استان لرستان، ایران جداسازی شدند. مطالعه مشخصات مرفولوژی و توالی یابی ناحیه‌های ژنی ITS-rDNA, tef1α آنها نشان داد که متعلق به هشت گونه Trichoderma هستند. بررسی تاثیر چهار نوع محیط کشت و پنج دما برای تعیین محیط کشت و دمای بهینه برای رشد و تکثیر این قارچ‌ها، نشان داد که محیط سیب زمینی/دکستروز/آگار(PDA) بهترین محیط و دمای 20 تا 30 درجه سلسیوس برای رشد و تکثیر این قارچ‌ها بهینه هستند. بررسی توانایی آنها در بازدارندگی از رشد قارچ خاکزی بیمارگر گیاهی Rhizoctonia solani در شرایط آزمایشگاهی، نشان داد که T. harzianum LT8 بیشترین توانایی بازدارندگی را دارد. بنابراین از این جدایه میتوان به عنوان یک عامل بالقوه مهارزیستی برای این قارچ بیمارگر گیاهی در پژوهش‌های آینده استفاده کرد.
واژه‌های کلیدی: بازدارندگی از رشد میسلیوم ITS-rDNA ، Rhizoctonia solani
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