Volume 12, Issue 1 ((Autumn & Winter) 2023)                   Plant Pathol. Sci. 2023, 12(1): 1-11 | Back to browse issues page

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Panahi Z, Khakvar R, Aliasgharzad N, Zehtab S, Farshabf PourAbad R. (2023). The effect of copper nanoparticles on soft rot agent of potato, carrot and onion. Plant Pathol. Sci.. 12(1), 1-11. doi:10.61186/pps.12.1.1
URL: http://yujs.yu.ac.ir/pps/article-1-382-en.html
The effect of copper nanoparticles on soft rot agent of potato, carrot and onion
Zahereh Panahi , Reza Khakvar * , Naser Aliasgharzad , Saeed Zehtab , Reza Farshabf PourAbad
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Abstract:   (1539 Views)

Panahi, Z., Khakvar, R., Aliasgharzad, N., Zehtab, S., & Farshbaf PourAbad, R. (2023). The effect of copper nanoparticles on soft rot agent of potato, carrot and onion. Plant Pathology Science, 12(1), 1-11.     

Introduction: Bacterial soft rot caused by Pectobacterium species is one of the important and common diseases in Potatoes and vegetables. Disinfection of tubers or seeds with chemicals is one of the methods of disease management. Copper nanoparticles, like silver and gold nanoparticles, have a strong inhibitory effect on bacterial cells, but they are much cheaper and more accessible than them. This research was conducted to determine the effect of copper nanoparticles alone and in combination with oxytetracycline and streptomycin antibiotics on potato, carrot and onion soft rot. Materials and Methods: The pathogen was isolated from rotten tissues of potato, carrot and onion, purified and identified by studying the phenotypic and genetic characteristics of the Pel-gene region using Pectobacterium specific primers (Y1 and Y2). The effect of copper nanoparticles, oxytetracycline and streptomycin, and their combination with copper nanoparticles on the pathogen growth was investigated in a completely randomized design experiment with three replications for each treatment in vitro. Results: Pectobacterium odoriferum was identified as pathogen based on phenotypic characteristics and genetic affinity. Streptomycin compared to oxytetracycline inhibited the pathogen growth more and their effect in combination with copper nanoparticles increased by 12 and 19.5%, respectively. Conclusion: Copper nanoparticles can inhibit the growth of P. odoriferum, and in combination with antibiotics increase their effect.

Keywords: Copper sulfate, Oxytetracycline, Pectobacterium, Streptomycin
Full-Text [PDF 583 kb]   (772 Downloads)    
Type of Study: Research | Subject: Special
Received: 2022/07/31 | Accepted: 2023/02/14
References
1. Agrios GN (2005) Plant Pathology. 3rd (ed.). Academic Press, New York. [DOI:10.1016/B978-0-08-047378-9.50007-5]
2. Agyemang PA, Kabir MN, Kersey CM, Dumenyo CK (2020) The bacterial soft rot pathogens, Pectobacterium carotovorum and P. atrosepticum, respond to different classes of virulence-inducing host chemical signals. Horticulturae 6:13. [DOI:10.3390/horticulturae6010013]
3. Ansari F (2014) Identification of bacterial causal agent of soft rot disease on potato and carrot tubers in BostanAbad Area. Master thesis, University of Tabriz. 117Pp. (In Persian)
4. Bahabadi NM, Hosseinpour Delavar F, Mirbakhsh M, Niknam KH, Johari SA (2015) Assessing antibacterial effect of filter media coated with silver nanoparticles against Bacillus spp. Iranian South Medical Journal 19:1-14. (In Persian with English Abstract)
5. Baras F, Van Gijsegem F, Chatterjee AK (1994) Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annual Review of Phytopathology 32:201-34. [DOI:10.1146/annurev.py.32.090194.001221]
6. Charkowski AO (2015) Biology and control of Pectobacterium in potato. American Journal of Potato Research 92:223-229. [DOI:10.1007/s12230-015-9447-7]
7. Ghazy NA, Abd El-Hafez OA, El-Bakery AM (2021) Impact of silver nanoparticles and two biological treatments to control soft rot disease in sugar beet (Beta vulgaris L.). Egyption Journal of Biological Pest Control 31:3-19. [DOI:10.1186/s41938-020-00347-5]
8. Gogos A, Knauer K, Bucheli TD (2012) Nanomaterials in plant protection and fertilization: current state, foreseen applications, and research priorities. Journal of Agricultural Food Chemmistry 39:9781-9792. [DOI:10.1021/jf302154y] [PMID]
9. Hoo CM, Starostin N, West P, Mecartney ML (2008) A comparison of atomic force microscopy (AFM) and dynamic light scattering (DLS) methods to characterize nanoparticle size distributions. Journal of Nanoparticle Research 10:89-96. [DOI:10.1007/s11051-008-9435-7]
10. Javadi A, Rostamirad A, Zand Monfared MR, Dastjani Farahani F, Heidarpour A, Khodadad Motlagh M (2015) The effect of ampicillin and gentamicin conjugated with gold nanoparticles on the formation of biofilms in Pseudomonas aeruginosa. Qom University of Medical Sciences Journal 9:35-41. (In Persian with English Abstract)
11. Marambio-Jones C, Hoek EMV (2010) A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. Journal of Nanoparticle Research 12:1531-1551. [DOI:10.1007/s11051-010-9900-y]
12. Marquez-Villavicencio MDP, Groves RL, Charkowski AO (2011) Soft rot disease severity is affected by potato physiology and pectobacterium taxa. Plant Disease 95:232-241. [DOI:10.1094/PDIS-07-10-0526] [PMID]
13. Naghash N, Safari M, Haimehrabi P (2012) Investigating the effect of silver nanoparticles on E. coli growth. Qom University of Medical Sciences Journal 6:65-68. (In Persian with English Abstract)
14. Panacek A, Kvítek L, Prucek R (2006) Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. The Journal of Physical Chemistry B 110:16248-16253. [DOI:10.1021/jp063826h] [PMID]
15. Ping L, Juan L, Changzhu W, Qingsheng W, Jian L (2005) Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles. Nanotechnology 16:1912-1917. [DOI:10.1088/0957-4484/16/9/082]
16. Portier P, Pédron J, Taghoutim G, Fischer-Le Saux M, Caullireau E, Bertrand C, Laurent A, Chawki K, Oulgazi S, Moumni M, Andrivon D, Dutrieux C, Faure D, Hélias V, Barny MA (2019) Elevation of Pectobacterium carotovorum subsp. odoriferum to species level as Pectobacterium odoriferum sp. nov., proposal of Pectobacterium brasiliense sp. nov. and Pectobacterium actinidiae sp. nov., emended description of Pectobacterium carotovorum and description of Pectobacterium versatile sp. nov., isolated from streams and symptoms on diverse plants. International Journal of Systematic and Evolutionary Microbiology 69:3207-3216. [DOI:10.1099/ijsem.0.003611] [PMID]
17. Ruparelia JP, Chatterjee AK, Duttagupta SP, Mukherji S (2008) Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater 4:701-716. [DOI:10.1016/j.actbio.2007.11.006] [PMID]
18. Schaad NW, Jones JB, Chun W (2001). Laboratory guide for identification of plant pathogenic bacteria Third Ed., APS Press, St. Paul, Minnesota USA, 373p.
19. Shaffiey SF, Ahmadi M, Shaffiey SR, Shapoori M, Varshouei SH, Azari F (2015) Synthesis of Copper Oxide (CuO) Nanoparticles and surveying its bactericidal properties against Aeromonas Hydrophila bacteria. Journal of Fasa University of Medical Sciences, 5:36-43
20. Vidaver AK (2002). Uses of Antimicrobials in Plant Agriculture. Clinical Infectious Diseases 34:107-110. [DOI:10.1086/340247] [PMID]
21. Yoon KY, Hoon Byeon J, Park JH, Hwang J (2007) Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles. Science of The Total Environment 373:572-576. [DOI:10.1016/j.scitotenv.2006.11.007] [PMID]
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