Volume 9, Issue 1 ((Spring and Summer) 2022)
Iranian J. Seed Res. 2022, 9(1): 1-24 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mehrabi Kooshki M, Moradi A, Balouchi H, Behboud R, Latifmanesh H. (2022). Germination and viability coefficients of Faba Bean (Vicia faba) seed under different storage conditions. Iranian J. Seed Res.. 9(1), : 1 doi:10.52547/yujs.9.1.1
URL: http://yujs.yu.ac.ir/jisr/article-1-516-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-516-en.html
Mohammad Mehrabi Kooshki 
, Ali Moradi * , Hamidreza Balouchi , Roya Behboud , Hojatollah Latifmanesh
, Ali Moradi * , Hamidreza Balouchi , Roya Behboud , Hojatollah Latifmanesh
Associate Professor of Agronomy and Plant Breeding Department, Yasouj University , amoradi@yu.ac.ir
Abstract: (2447 Views)
Extended Abstract
Introduction: Pulses are among the best sources of plant protein and important components of crop rotation, which in recent years, have been considered one of the major options for plant research. Seed storage is one of the important traits in legume breeding. Storage temperature, seed moisture content, and storage duration are the most important factors affecting seed quality during storage. Inappropriate storage conditions lead to deterioration and reduction of seed quality during storage, which is severely affected by the environmental conditions of storage.
Materials and Methods: This research was conducted at the Seed Technology Laboratory, Faculty of Agriculture, Yasouj University in 2014 as a three-way factorial based on the completely randomized design with 5 replications of 20 seeds. Seeds with moisture content at 5 levels (6, 10, 14, 18, and 22%) and storage temperature at 4 levels (15, 25, 35 and 45 °C) were stored for 9 months (0, 30, 60, 90, 120, 150, 180, 210, 240 and 270 days). After sampling at the end of each month, a standard seed germination test was done using the pleated paper method in a germinator at 25 °C for 10 days. Also, an electrical conductivity test of the electrolytes leaked from the seeds incubated for 24h in water at 20 ˚C was done with 4 replicates. Some germination attributes and electrical conductivity of the electrolytes leaked from the seeds were measured according to standard methods.
Results: According to the results, interaction effects of storage temperature, seed moisture content, and storage duration on germination indices and electrical conductivity of bean seeds were significant (P<0.1). The germination trend during storage at 15 °C and seed moisture content of 6% decreased from 94% to 81% after 270 days of storage, so that germination decreased to 35% under similar moisture content after 270 days of storage as temperature increased from 15 to 45 °C. As the storage time passed, electrical conductivity increased and this increase was more pronounced at higher temperatures. Viability constants were calculated 9 months after storage using the seed viability equation, in which KE, CH, CW, and CQ were calculated -5.39697, 0.03201, 2.13041, and 0.000017, respectively.
Conclusions: The results showed that the electrical conductivity of the leaked material increased with increasing storage temperature and seed moisture content, which led to lower viability of seeds. At 15 °C and 6% seed moisture content provided better conditions for seed survival during the 9-month storage time compared with all other temperatures and moistures and had the lowest rate of deterioration. The results showed that with increasing seed temperature and moisture so that they had to lowest electrical conductivity of the leaked material from seeds and deterioration rate.
Highlights:
1- Over storage duration, the electrical conductivity of materials leaked from seeds increased.
2- With increasing moisture content up to 22% and storage temperature up to 45 °C, the electrical conductivity of the material leaked from seeds increased.
3- Bean seed viability coefficients were calculated to evaluate seed viability under controlled storage conditions.
Introduction: Pulses are among the best sources of plant protein and important components of crop rotation, which in recent years, have been considered one of the major options for plant research. Seed storage is one of the important traits in legume breeding. Storage temperature, seed moisture content, and storage duration are the most important factors affecting seed quality during storage. Inappropriate storage conditions lead to deterioration and reduction of seed quality during storage, which is severely affected by the environmental conditions of storage.
Materials and Methods: This research was conducted at the Seed Technology Laboratory, Faculty of Agriculture, Yasouj University in 2014 as a three-way factorial based on the completely randomized design with 5 replications of 20 seeds. Seeds with moisture content at 5 levels (6, 10, 14, 18, and 22%) and storage temperature at 4 levels (15, 25, 35 and 45 °C) were stored for 9 months (0, 30, 60, 90, 120, 150, 180, 210, 240 and 270 days). After sampling at the end of each month, a standard seed germination test was done using the pleated paper method in a germinator at 25 °C for 10 days. Also, an electrical conductivity test of the electrolytes leaked from the seeds incubated for 24h in water at 20 ˚C was done with 4 replicates. Some germination attributes and electrical conductivity of the electrolytes leaked from the seeds were measured according to standard methods.
Results: According to the results, interaction effects of storage temperature, seed moisture content, and storage duration on germination indices and electrical conductivity of bean seeds were significant (P<0.1). The germination trend during storage at 15 °C and seed moisture content of 6% decreased from 94% to 81% after 270 days of storage, so that germination decreased to 35% under similar moisture content after 270 days of storage as temperature increased from 15 to 45 °C. As the storage time passed, electrical conductivity increased and this increase was more pronounced at higher temperatures. Viability constants were calculated 9 months after storage using the seed viability equation, in which KE, CH, CW, and CQ were calculated -5.39697, 0.03201, 2.13041, and 0.000017, respectively.
Conclusions: The results showed that the electrical conductivity of the leaked material increased with increasing storage temperature and seed moisture content, which led to lower viability of seeds. At 15 °C and 6% seed moisture content provided better conditions for seed survival during the 9-month storage time compared with all other temperatures and moistures and had the lowest rate of deterioration. The results showed that with increasing seed temperature and moisture so that they had to lowest electrical conductivity of the leaked material from seeds and deterioration rate.
Highlights:
1- Over storage duration, the electrical conductivity of materials leaked from seeds increased.
2- With increasing moisture content up to 22% and storage temperature up to 45 °C, the electrical conductivity of the material leaked from seeds increased.
3- Bean seed viability coefficients were calculated to evaluate seed viability under controlled storage conditions.
Article number: 1
Type of Study: Research |
Subject:
Seed Physiology
Received: 2021/10/13 | Revised: 2024/02/21 | Accepted: 2022/03/14 | ePublished: 2022/12/11
Received: 2021/10/13 | Revised: 2024/02/21 | Accepted: 2022/03/14 | ePublished: 2022/12/11
References
1. Akramian, M., Hosseini, A., Kazerooni, M. and Rezvani, M.J. 2007. Effect of seed osmopriming on germination and seedling development of fennel (Foeniculum vulgare Mill.). Iranian Journal of Field Crop Research, 5(1): 37-46. [In Persian with English Summary].
2. Alivand, R. 2011. Investigation of seed deterioration in oil seed plants with different storage conditions. Master Thesis of Tehran University. 208p.
3. Alivand, R., Tavakkol Afshari, R. and Sharif-Zadeh, F. 2013. Germination response and estimation of seed deterioration of Brassica napus under various storage conditions. Iranian Journal of Field Crop Science, 43: 21-46. [In Persian with English Summary].
4. Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Science Research, 14: 93-107. [DOI:10.1079/SSR2004159]
5. Baladi, S. 2014. Evaluation of Seed longevity and viability coefficients of seeds of two cultivars of linseed oil (Linum usitatissimum L.) and one cultivar of balango (Lallemantia royleana) in different storage conditions and the effect of priming in repairing its deterioration. Master Thesis of Yasouj University. 165p.
6. Balouchi, H.R., Bagheri, F., Kayednezami, R., Movahhedi Dehnavi, M. and Yadavi, A.R. 2013. Effect of seed aging on germination and seedling growth indices in three cultivars of Brassica napus L. Journal of Plant Research (Iranian Journal of Biology), 26(4): 397-411. [In Persian with English Summary].
7. Basra, S.M., Ullah, E., Warriach, E., Cheema, M. and Afzal, I. 2003. Effect of storage on growth and yield of primed canola (Brassica napus) seeds. International Journal of Agriculture and Biology, 5(2): 117-120.
8. Bayat, P., Ghobadi, M., Ghobadi, M. and Mohammadi, G. 2016. Evaluation of standard seed germination capability in vitro to predict the appearance and establishment of chickpea seedling (Cicer arietinum L.) in the field. Iranian Journal of Seed Science and Technology, 5(1): 27-38. [In Persian with English Summary].
9. Biabani, A., Boggs, L.C., Katozi, M. and Sabouri, H. 2011. Effects of seed deterioration and inoculation with Mesorhizobium ciceri on yield and plant performance of chickpea. Australian Journal of Crop Science, 5(1): 66-70.
10. Bradford, K.J. 2004. Seed production and quality. California, USA, 138p.
11. Dikie, J.B., Ellis, R.H., Kraak, H.L., Ryder, K. and Tompsett, P.B. 1990. Temperature and seed storage longevity. Annals of Botany, 65: 197-204. [DOI:10.1093/oxfordjournals.aob.a087924]
12. Ellis, R.H. and Roberts, E.H. 1980. Improved equations for the prediction of seed longevity. Annals of Botany, 45: 13-30. [DOI:10.1093/oxfordjournals.aob.a085797]
13. Ghaderi-Far, F. Soltani, A. and Sadeghipor, H.R. 2010. Determination of seed viability constant value in paper squash, borage and black seed. Journal of Plant Research (Iranian Journal of Biology), 7(3): 53-66. [In Persian with English Summary].
14. Ghasemi-golezani, K., Chadordooz-jeddi, A., Nasrullahzade, S. and Moghaddam, M. 2010. Influence of hydro-priming duration on field performance of pinto bean (Phaseolus vulgaris L.) cultivars. African Journal Agricultural Research for Development, 5(9): 893-897.
15. Goel, A. and Sheoran, I.S. 2003. Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198.
16. Hampton, J.G. and TecKrony, D.M. 1995. Handbook of vigor test methods. The International Seed Testing Association, Zurich, p.117.
17. Harrington, J.F. 1972. Seed storage and longevity. P.145-245. In: T.T. Kozlowski (ed.). Seed biology. Vol3. Academic Press. New York. [DOI:10.1016/B978-0-12-395605-7.50009-0]
18. Hashemi, A., Tavakkol Afshari, R., Tabrizi, L. and Barooti, Sh. 2018. The modeling of Plantago ovate seed viability under various storage conditions. Iranian Journal of Seed Science and Technology, 7(2): 95-104. [In Persian with English Summary].
19. Hong, T.D., Ellis, R.H. and Moor, D. 1997. Development of a model to predict the effect of temperature and moisture content on fungal spore longevity. Annals of Botany, 79: 121-128. [DOI:10.1006/anbo.1996.0316]
20. Hung, L.Q., Hong, T.D. and Ellis, R.H. 2001. Constant, fluctuating and effective temperature and seed longevity: a tomato (Lycopersicone sculentum Mill.) example. Annals of Botany, 88: 465-470. [DOI:10.1006/anbo.2001.1487]
21. International Seed Testing Association (ISTA). 2010. International rules for seed testing. Bassersdorf, Switzerland.
22. Jafari, Z. 2014. Temperature-humidity response of Fennel (Foeniculum vulgare Mill) under different storage conditions. Master Thesis of Tehran University. 89p. [In Persian with English Summary].
23. Kapoor, N., Aria, A., Siddiqui, M.A. Amir, A. and Kumar, H. 2010. Seed deterioration in chickpea (Cicer arietinum L.) under accelerated ageing. Asian Journal of Plant Sciences, 9: 158-162. [DOI:10.3923/ajps.2010.158.162]
24. McDonald, M.B. 1999. Seed deterioration physiology, repair and assessment. Seed Science and Technology, 27: 177-237.
25. Miller, T. and Chapman, S.J. 1978. Germination responses of three forage grasses to different concentration of six salts. Journal of Range Management, 31(2): 123-124. [DOI:10.2307/3897659]
26. Mirdad, Z., Powell, A.A. and Matthews, S. 2006. Prediction of germination in artificially aged seeds of Brassica spp using the bulk conductivity test. Seed Science and Technology, 24: 328-340. [DOI:10.15258/sst.2006.34.2.03]
27. Moeinzadeh, A., Tavakkol Afshari, R., Moghadam, H. and Baghizadeh, A. 2018. The effect of storage conditions on seed germination indices and viability constant of lentil (Lens culinaris) and pea (Cicer arientinum) seed. Iranian Journal of Field Crop Science, 49(2): 71-92. [In Persian with English Summary].
28. Mohammadi, H., Soltani, A., Sadeghipour H.R. and Zeinali, H. 2011. Effects of seed aging on subsequent seed reserve utilization and seedling growth in soybean. International Journal of Plant Production, 5(1): 65-70.
29. Pagter, M., Bragato, C., Malagoli, M. and Brix, H.J. 2009. Osmotic and ionic effects of NaCl and Na2So4 salinity on Phragmites australis. Aquatic Botany, 90(1): 43-51. [DOI:10.1016/j.aquabot.2008.05.005]
30. Pradidwong, S., Isarasenee, A. and Pawelzik, E. 2004. Prediction of mungbean content and storage temperature. Deutscher Tropentag, October 5-7, Berlin seed longevity and quality using the relationship of seed moisture, p.325.
31. Rahemi Karizki, A., Nakhzari Moghadam, A. and Pourabdollah, M. Effect of seed vigour on germination and heterotrophic growth of wheat in response to salinity. Iranian Journal of Seed Science and Technology, 2(2): 60-67. [In Persian with English Summary].
32. Rastegar, Z., Sedghi, M. and Khomari, S. 2011. Effects of accelerated aging on soybean seed germination indexes at laboratory conditions. Notulae Scientia Biologicae, 3(3): 126-129. [DOI:10.15835/nsb336075]
33. Roberval, D. and Maristela, P. 2007. Electrical conductivity and deterioration of soybean seeds exposed to different storage conditions. Revista Brasileira de Sementes, 29: 97-105. [DOI:10.1590/S0101-31222007000200013]
34. Schmidt, L. 2007. Tropical forest seed. Springer-Verlag Heidelberg New York. [DOI:10.1007/978-3-540-68864-8]
35. Seiadat, S.A., Moosavi, A. and Sharafizadeh, M. 2012. Effect of seed priming on antioxidant activity and germination characteristics of maize seeds under different aging treatments. Research Journal of Seed Science, 5(2): 51-62. [DOI:10.3923/rjss.2012.51.62]
36. Sharifzadeh, F. and Dehghan, M. 2012. The estimation of viability equation in seeds of perennial rye (Secale montanum) under different conditions of temperature and moisture content. Agronomy Journal (Pajouhesh and Sazandegi), 94: 16-22. [In Persian with English Summary].
37. Soltani, M. 2015. Seed germination modelling and estimation of seed viability coefficients in castor bean (Ricinus communis L.) seeds under different storage conditions. Master Thesis of Yasouj University. 99p. [In Persian with English Summary].
38. Tabatabei, A. 2014. Determination of barley seed viability coefficients under different storage conditions. Seed Research Journal, 4(2): 13-20. [In Persian with English Summary].
39. Tang, S., Tekriny, D.M., Egli, D.B. and Cornelius, P.L. 2000. An alternative model to predict corn seed deterioration during storage. Crop Science, 40: 463-470. [DOI:10.2135/cropsci2000.402463x]
40. Turpin, J.E., Robertson, M.J., Hillcoat, N.S. and Herridge, D.F. 2002. Faba bean (Vicia faba L.) in Australia's northern grains belt: canopy development, biomass, and nitrogen accumulation and partitioning. Australian Journal of Agricultural Research, 53(2): 227-237. [DOI:10.1071/AR00186]
41. Usberti, R. 2007. Performance of tropical forage grass (Brachiaria brizantha) dormant seed under controlled storage. Seed Science and Technology, 35: 402-413. [DOI:10.15258/sst.2007.35.2.15]
42. Vieira, R.D., Penariol, A.L., Perecin, D. and Panobianco, M. 2002. Condutividadeeletrica e teor de aguainicial das sementes de soja. Pesquisa Agropecuaria Brasileira, 37(9): 1333-1338. [DOI:10.1590/S0100-204X2002000900018]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
