Volume 12, Issue 1 ((Autumn & Winter) 2023)                   pps 2023, 12(1): 53-63 | Back to browse issues page

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Akhlaghi M, Alymanesh M R, Rohani A, Barjasteh A R, Abedini Esfahlani M, Dezyania A. (2023). The impact of two active ingredients of plant essential oils on the causative agent of apple and pear fire blight disease. pps. 12(1), 53-63.
URL: http://yujs.yu.ac.ir/pps/article-1-391-en.html
epartment of Plant Protection, Faculty of Agriculture, Ilam University, Ilam, Iran , m.alimanesh@ilam.ac.ir
Abstract:   (465 Views)
Akhlaghi, M., Alymanesh, MR., Rohani, A., Barjasteh, A., and Abedini Esfahlani, M., Dezianian, A. (2023) The impact of two active ingredients of plant essential oils on the causative agent of apple and pear fire blight disease. Plant Pathology Science, 12(1), 53-63.       Doi: 10.2982/PPS.12.1.53
Introduction: Erwinia amylovora, the cause of apple and pear fire blight disease, is one of the main factors limiting the production of these fruits in the world. Since only a limited number of copper compounds have a protective effect in controlling this disease, increasing attention has been paid to investigating the effect of plant essential oils on this pathogen. Materials and Methods: The effect of different concentrations of carvacrol (the active ingredient of thyme, oregano, etc.) and trans-anethole (the active ingredient of fennel essential oil) on the growth of E. amylovora colony, was investigated in laboratory conditions, and their minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined. The inhibitory effect of MIC of these substances alone and the mixture of one-half of their MIC and MBC concentrations was investigated by disc method on nutrient agar medium on the growth of E. amylovora colony. The effect of the three best mixing ratios of these materials on the fruit tissue of the pear Spadona variety was evaluated on the severity of fire blight disease. Results: Carvacrol and trans-anethole both had antibacterial effects against E. amylovora, but the effect of carvacrol was greater than the other. Mixing carvacrol with trans-anethole increased its effectiveness. Among the three mixing ratios, 47 µg/ml of carvacrol + 141 µg/ml of trans-anethole caused the greatest reduction in disease severity in pear fruit tissue. Conclusion: The use of a mixture of carvacrol and trans-anethole can be considered for the management of fire blight disease.


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Type of Study: Research | Subject: Special
Received: 2022/10/20 | Accepted: 2023/02/6

1. Adaskaveg JE, Gubler WD (2008) Evaluation of new bactericides for control of fire blight of pears caused by Erwinia amylovora. Pp. 75- 82. In: California Pear Research Report 2008. California Pear Advisory Board. Online Publication.
2. Akhlaghi M, Tarighi S, Taheri P (2020) Effect of antimicrobial peptides and monoterpenes on control of fire blight. Spanish Journal of Agricultural Research 18:1-15. [DOI:10.5424/sjar/2020182-15629]
3. Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods- a review. International Journal of Food Microbiology 94:223-253. [DOI:10.1016/j.ijfoodmicro.2004.03.022] [PMID]
4. Chiriac I, Ulea E (2012) In vitro susceptibility of Erwinia amylovora (Burrill) Winslow et al. strain isolated from pear to several plant extracts and different pesticides. Cercetări Agronomice in Moldova 1:149. [DOI:10.2478/v10298-012-0006-9]
5. Dagher F, Olishevska S, Philion V, Zheng J, Deziel E (2020). Development of a novel biological control agent targeting the phytopathogen Erwinia amylovora. Heliyon 6:e05222. [DOI:10.1016/j.heliyon.2020.e05222] [PMID] [PMCID]
6. Fischer TC, Gosch C, Mirbeth B, Gselmann M, Thallmair V, Stich K (2012) Potent and specific bactericidal effect of juglone (5-Hydroxy-1, 4- naphthoquinone) on the fire blight pathogen Erwinia amylovora. Journal of Agricultural and Food Chemistry 60:12074−12081. [DOI:10.1021/jf303584r] [PMID]
7. Fontana R, Macchi G, Caproni A, Sicurella M, Buratto M, Salvatori F, Pappadà M, Manfredini S, Baldisserotto A, Marconi P (2022). Control of Erwinia amylovora Growth by Moringa oleifera Leaf Extracts: In Vitro and in Planta Effects. Plants, 11:1-19. [DOI:10.3390/plants11070957] [PMID] [PMCID]
8. Gusberti M, Klemm U, Meier MS, Maurhofer M, Hunger-Glaser, I (2015). Fire blight control: the struggle goes on. A comparison of different fire blight control methods in Switzerland with respect to biosafety, efficacy and durability. International Journal of Environmental Research and Public Health 12:11422-11447. [DOI:10.3390/ijerph120911422] [PMID] [PMCID]
9. Hong JC, Momol MT, Ji P, Olson SM, Colee J, Jones JB (2011) Management of bacterial wilt in tomatoes with thymol and acibenzolar-S-methyl. Crop Protection 30:1340-1345. [DOI:10.1016/j.cropro.2011.05.019]
10. Iacobellis NS, Lo Cantore P, Capasso F, Senatore F, (2005) Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils. Journal of Agricultural and Food Chemistry 53:57-61. [DOI:10.1021/jf0487351] [PMID]
11. Kotan R, Dadasoglu F, Kordali S, Cakır A, Dikbas N, Cakmakc R (2007) Antibacterial activity of essential oils extracted from some medicinal plants, carvacrol and thymol on Xanthomonas axonopodis pv. vesicatoria (Doidge) dye causes bacterial spot disease on pepper and tomato. Journal of Agricultural Technology 3:299-306.
12. Magi G, Marini E, Facinelli B (2015) Antimicrobial activity of essential oils and carvacrol and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci. Front Microbiol. 6:165. [DOI:10.3389/fmicb.2015.00165] [PMID] [PMCID]
13. Marofzadeh N, Mohammadi M, Sharifi Tehrani A, Zakeri Z (2009) A study of the efficiency of some serological and PCR-based methods for the identification and detection of Erwinia amylovora, the causal agent of pome trees fire blight. Iranian Journal of Plant Protection Science 40:55-64. (In Persian with English Abstract)
14. Nostro A, Papalia T (2012) Antimicrobial activity of carvacrol: current progress and future prospectives. Recent Patents on Anti-Infective Drug Discovery 7:28-35. [DOI:10.2174/157489112799829684] [PMID]
15. Obey KJ, Wright VA, Orjala J, Kauhanen J, Tikkanen-Kaukanen C (2016) Antimicrobial activity of Croton macrostachyus stem bark extracts against several human pathogenic bacteria. Journal of Pathogens 10:1-5. [DOI:10.1155/2016/1453428] [PMID] [PMCID]
16. Ommati F, Zaker M (2014) Role of infected plant parts in development and survival of fire blight of pome fruits (Erwinia amylovora). Journal of Applied Research in Plant Protection 3:57-68. (In Persian with English Abstract)
17. Rezaei R (2020) Effect of eight essential oils on bacterial canker disease in citrus. Plant Pathology Science 9:30-39. (In Persian with English Abstract) [DOI:10.29252/pps.9.1.30]
18. Rita Proto M, Biondi E, Baldo D, Levoni M, Filippini G, Modesto M, Di Vito M, Bugli F, Ratti C, Minardi P, Mattarelli P (2022) Essential Oils and Hydrolates: Potential Tools for Defense against Bacterial Plant Pathogens. Microorganisms 10:1-19. [DOI:10.3390/microorganisms10040702] [PMID] [PMCID]
19. Salami M, Rahimmalek M, Ehtemam MH, Szumny A, Fabian S, Matkowski A (2015) Essential oil composition, antimicrobial activity and anatomical characteristics of Foeniculum vulgare Mill. fruits from different regions of Iran. Journal of Essential Oil-Bearing Plants 19:7 [DOI:10.1080/0972060X.2015.1117951]
20. Sokovic M, Van Griensven LJLD (2006) Antimicrobial activity of essential oils and their components against the three major pathogens of the cultivated button mushroom, Agaricus bisporus. European Journal of Plant Pathology 116:211-224. [DOI:10.1007/s10658-006-9053-0]
21. Wang C, Fan Y (2014) Eugenol enhances the resistance of tomato against tomato yellow leaf curl virus. Journal of the Science of Food and Agriculture 94:677-82. [DOI:10.1002/jsfa.6304] [PMID]
22. Wieczyńska J, Cavoski I (2018) Antimicrobial, antioxidant and sensory features of eugenol, carvacrol and trans-anethole in active packaging for organic ready-to-eat iceberg lettuce. Food Chemistry 259:251-260. [DOI:10.1016/j.foodchem.2018.03.137] [PMID]
23. Zhao Y, Wang D, Nakka S, Sundin GW, Korban SS (2009) Systems level analysis of two-component signal transduction systems in Erwinia amylovora: Role in virulence, regulation of amylovoran biosynthesis and swarming motility. BMC Genomics 10:1-16. [DOI:10.1186/1471-2164-10-245] [PMID] [PMCID]

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