Volume 11, Issue 1 ((Autumn & Winter) 2022)                   pps 2022, 11(1): 1-12 | Back to browse issues page


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Pakbaz S, Darvishnia M, Naghavi A. 1-Phylogenetic status of Grapevine fanleaf virus isolate of Lorestan province of Iran. pps. 2022; 11 (1) :1-12
URL: http://yujs.yu.ac.ir/pps/article-1-363-en.html
Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran , samira.pakbaz@gmail.com
Abstract:   (351 Views)
Pakbaz S, Darvishnia M, Naghavi A (2022) Phylogenetic status of Grapevine fanleaf virus isolate of Lorestan province of Iran. Plant Pathology Science 11(1):1-12.                     Doi: 10.2982/PPS.11.1.1
 
Introduction: Grapevine fanleaf virus (GFLV) is a major grapevine infecting virus in the world. Materials and Methods: Grapes showing GFLV signs were sampled from the suburbs of the city of Khorramabad in Lorestan Province of Iran in spring 2020 and RT-PCR test was performed to amplify their GFLV-CP gene and the product was sequenced. Results: Specific primers were able to amplify a 1515 bp fragment of the CP gene. Based on the nucleotide sequence of this fragment, GFLV was first identified in this region. The nucleotide sequence similarity of this isolate was detected at 89.14-95.64% with other isolates in the NCBI library. Also, the phylogenetic tree of these isolates, based on the genomic CP region, grouped GFLV isolates into two groups I and II. The Lorestan GFLV isolate was placed in a subgroup in Group I together with GFLV isolates from the northwestern part of Iran and the Takestan isolate, and isolates from other countries were grouped in a separate subgroup of this group. Also in the phylogenetic tree, the Northeast isolates and the Fars and Kohgiloyeh & Boyer-Ahmad province isolates were classified in Group II. Conclusion: The results of this research indicate that the virus is endemic and that its likely origin was in Iran and then spread to other parts of the world. The impact of geographic segregation on the evolution of GFLV can also be deduced.

 
Keywords:  GFLV, RT-PCR, Sequencing
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Type of Study: Research | Subject: Special

References
1. Andert-Link P, Laporte C, Valat L, Ritzenthaler C, Demangeat G, Ving E, Laval V, Pfeiffer P, Stussi-Garaud C, Fuchs M (2004) Grapevine fanleaf virus: still a major threat to the grapevine industry. Journal of Plant Pathology 86:183-195.
2. Fuchs M (2020) Grapevine viruses: a multitude of diverse species with simple but overall poorly adopted management solutions in the vineyard. Journal of Plant Pathology 102:643-653. [DOI:10.1007/s42161-020-00579-2]
3. Gharouni S, Karimishahri M, Azaddisfani F (2022) Distribution and Phylogeny of Grapevine fanleaf virus in Vineyards of Khorasan Razavi Province Based on Coat Protein Region, Journal of Plant Protection 35:421-431. (In Persian with English Abstract).
4. Gholampour Z, Kargar M, Zakiaghl M, Siampour M, Mehrvar M, Izadpanah K (2017) Dynamics of the population structure and genetic variability within Iranian isolates of Grapevine fanleaf virus: evidence for polyphyletic origin. Acta Virologica, 61:324-335. [DOI:10.4149/av_2017_311] [PMID]
5. Gholampour Z, Zaki-Aghl M (2016) Comparison of RNA Extraction Methods for the Identification of Grapevine fanleaf virus. Journal of Plant Protection 30:127-133. (In Persian with English Abstract).
6. Izadpanah K, Zaki-Aghl M, Zhang Y, Daubert S, Rowhani A (2003) Bermuda grass as a potential reservoir host for Grapevine fanleaf virus. Plant Disease 87:1179-1182. [DOI:10.1094/PDIS.2003.87.10.1179] [PMID]
7. Kargar M, Zakiaghl M, Masoumi M, Mehrvar M, Izadpanah K (2017) Analysis of genetic diversity of Grapevine fanleaf virus isolate from Fars and Kohgiluyeh-Boyer Ahmad provinces. Iranian Journal of Plant Pathology 52:375-391. (In Persian with English Abstract).
8. Martelli GP, Savino V (1990) Fanleaf Degeneration. PP.48-49. In: RC Pearson, A Goheen (eds.). Compendium of Grape Diseases. APS Press, St. Paul, MN, USA.
9. Moradi R, Koolivand D, Eini O, Hajizadeh M (2018) Molecular Identification of four important Nepovirus from vineyards of Zanjan province, Iranian Journal of Plant Protection Science 49:99-110. (In Persian with English Abstract).
10. Nourinezhad Zarghani SH, Shams-Bakhsh M, Sokhandan-Bashir N, Pazhouhandeh M (2012) Identification and detection of Iranian isolates of Grapevine fanleaf virus using green-grafting and RT-PCR. Iranian Journal of Plant Pathology 48:381-391. (In Persian with English Abstract).
11. Panno S, Caruso AG, Bertacca S, Pisciotta A, Lorenzo RD, Marchione S, Matić S, Davino S (2021) Genetic structure and molecular variability of Grapevine fanleaf virus in Sicily. Agriculture 11:496. [DOI:10.3390/agriculture11060496]
12. Parvizi R (1989) Incidence of Grapevine fanleaf virus in Uromia. Procced 9th Plant Protection Congress. Iran, Mashhad. P.164.
13. Pourrahim R, Farzadfar S, Golnaraghi AR, Ahoonmanesh A (2007) Partial molecular characterization of some Grapevine fanleaf virus isolates from North-east of Iran. Journal of Phytopathology 155:754-757. [DOI:10.1111/j.1439-0434.2007.01299.x]
14. Rakhshandehroo F, Pourrahim R, Zamanizadeh H, Rezaee S, Mohammadi M (2005) Incidence and distribution of viruses Infecting Iranian vineyards. Journal of Phytopathology 153:480-484. [DOI:10.1111/j.1439-0434.2005.01006.x]
15. Reisch BI, Owens CL, Cousins PS (2012) Grape. In Fruit Breeding. Springer, Boston, MA. 225-262. [DOI:10.1007/978-1-4419-0763-9_7]
16. Sanfacon H, Wellink J, Le Gall O, Karasev A, Vlugt vander R, Wetzel T (2009) Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus. Archives of Virology 154:899-907. [DOI:10.1007/s00705-009-0367-z] [PMID]
17. Sokhandan-Bashir N, Melcher U (2012) Population genetic analysis of Grapevine fanleaf virus. Archives of Virology 157:1919-1929. [DOI:10.1007/s00705-012-1381-0] [PMID]
18. Vuittenez A (1970) Fanleaf of Grapevine. Pp.217-228. In: NW Frazier (ed.). Virus Disease of Small Fruits and Grapevine. University of California, Berkley. -----------

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