Volume 11, Issue 1 ((Autumn & Winter) 2022)                   pps 2022, 11(1): 89-99 | Back to browse issues page


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Abootorabi E, Ebrahimi L. 8- Introduction of three native entomopathogenic nematodes of Iran and their impact on honeycomb moth. pps. 2022; 11 (1) :89-99
URL: http://yujs.yu.ac.ir/pps/article-1-350-en.html
Department of Nematology, Iranian Research Institute of Plant Protection, Tehran, Iran. , elabootorabi@gmail.com
Abstract:   (328 Views)
Abootorabi E, Ebrahimi L (2022) Introduction of three entomopathogenic nematodes of Iran and their impact on honeycomb moth. Plant Pathology Science 11(1):89-99.        
 Doi: 10.2982/PPS.11.1.89.
 
Introduction: The aim of this study was to collect and identify entomopathogenic nematodes native to Iran and to evaluate their pathogenicity on honeycomb moth larvae (Galleria mellonella). Materials and Methods: Thirteen isolates of entomopathogenic nematodes were collected from different provinces of Iran and identified based on morphological characters. The percentage mortality of G. mellonella larvae infected with these isolates at 25 ± 1 and 32 ± 1 °C was determined in a one-to-one assay, and the ability of the isolates to find a target and the mortality of the insect in the sand column test were determined. Results: Seven isolates of Heterorhabditis bacteriophora, two isolates of Steinernema feltiae, and five isolates of S. carpocapsae were identified. The ability of isolates of all three nematode species to penetrate the insect's body has been shown to be up to 93% within 48-72 hours post-infection at 25 ± 1°C. The optimum temperature for the biological activity of the identified isolates was 25±1°C. S. carpocapsae found a target faster than the other two species in the sand column test. Conclusion: Isolates of S. carpocapsae have higher potential in targeting and pathogenicity of honeycomb moth larvae than the other two nematode species.

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