Volume 11, Issue 1 ((Autumn & Winter) 2022)                   pps 2022, 11(1): 89-99 | Back to browse issues page

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Abootorabi E, Ebrahimi L. (2022). 8- Introduction of three native entomopathogenic nematodes of Iran and their impact on honeycomb moth. pps. 11(1), 89-99. doi:10.52547/pps.11.1.89
URL: http://yujs.yu.ac.ir/pps/article-1-350-en.html
Department of Nematology, Iranian Research Institute of Plant Protection, Tehran, Iran. , elabootorabi@gmail.com
Abstract:   (1392 Views)
Abootorabi E, Ebrahimi L (2022) Introduction of three entomopathogenic nematodes of Iran and their impact on honeycomb moth. Plant Pathology Science 11(1):89-99.        
 Doi: 10.2982/PPS.11.1.89.
Introduction: The aim of this study was to collect and identify entomopathogenic nematodes native to Iran and to evaluate their pathogenicity on honeycomb moth larvae (Galleria mellonella). Materials and Methods: Thirteen isolates of entomopathogenic nematodes were collected from different provinces of Iran and identified based on morphological characters. The percentage mortality of G. mellonella larvae infected with these isolates at 25 ± 1 and 32 ± 1 °C was determined in a one-to-one assay, and the ability of the isolates to find a target and the mortality of the insect in the sand column test were determined. Results: Seven isolates of Heterorhabditis bacteriophora, two isolates of Steinernema feltiae, and five isolates of S. carpocapsae were identified. The ability of isolates of all three nematode species to penetrate the insect's body has been shown to be up to 93% within 48-72 hours post-infection at 25 ± 1°C. The optimum temperature for the biological activity of the identified isolates was 25±1°C. S. carpocapsae found a target faster than the other two species in the sand column test. Conclusion: Isolates of S. carpocapsae have higher potential in targeting and pathogenicity of honeycomb moth larvae than the other two nematode species.

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Type of Study: Research | Subject: Special
Received: 2021/11/21 | Accepted: 2022/04/26

1. Abootorabi E (2011). The Control Feasibility of Ectomyelois ceratoniae by Entomopathogenic nematode. National Pomegranate Symposium, Ferdows, Iran. P.28.
2. Abootorabi E , Farrokhi Sh (2019). Efficacy of a native isolate of Steinernema feltiae on tomato leafminer (Tuta absoluta) under laboratory and greenhouse conditions. Biocontrol in Plant Protection 6:31-41.
3. Adams BJ, Nguyen KB (2002). Toxonomy and Systematyics. PP.1-34. In: R Gaugler (ed.). Entomopathogenic Nematology. CABI Publishing.
4. Akhurts RJ, Boemare NE (1990). Biology and Taxonomy of Xenorhabdus. PP. 75-90. In: R Gaugler, H K Kaya (eds.). Entomopathogenic Nematodes in Biological Control. CRC Press, Boca Raton. [DOI:10.1201/9781351071741-5]
5. Bedding R, Molyneux A (1982). Penetration of insect cuticle by infective juveniles of Heterorhabditis spp. (Heterorhabditidae: Nematoda). Nematologica 28:354-359. [DOI:10.1163/187529282X00402]
6. Brown L, Gaugler R (1997). Temparature and humidity influence emergence and survival of entomophatogenic nematodes. Nematologica 43:363-375. [DOI:10.1163/005025997X00102]
7. De Grisse A (1969). Redescription ou modifications de quelques techniques utlisees dans L' etude Nematodes Phytoparasitaires. Meded, Rijksfaculteit der landbouwe_ tenschappen. Gent 34:251-369.
8. Ebrahimi L, Niknam G (2012). Detection of termal preference range of two endemic isolates of entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora for application in biological control of insect pests. Journal of Agricultural Science and Sustainable Production 21:77-86.
9. Grewal PS, Ehler RU, Shapiro- Ilan D I (2008). Nematode as Biocontrol Agents. CABI, Wallingford, UK, 528p.
10. Griffin CT, Downes MJ (1994). Recognation of low temperature active isolates of the entomopathogenic nematode Heterorhabditis. Nematologica 37:83-91. [DOI:10.1163/187529291X00088]
11. Hazir S, Stock SP, Kaya HK, Koppenhofer AM, Keskin N (2001). Developmental temperature effects on five geographic isolates of entomopathogenic nematode Steinernema feltiae. Journal of Invertebrate Pathology 77:243-250. [DOI:10.1006/jipa.2001.5029] [PMID]
12. Miller R (1989). Novel pathogenicity assessment technique of Steinernematids and Heterorhabditis entomopathogenic nematodes. Journal of Nematology 21:574.
13. Parvizi R, Barooti Sh, Adldoost H (1994). Report on insect parasitic nematodes in Iran. Journal of Plant Pests and Diseases 62:109.
14. Poinar GO (1975). Description and biology of a new insect parasitic rhabditoid, Heterorhabditis bacteriophora n. gen., n. sp. (Rhabditida: Heterorhabditidae n. fam.). Nematologica 21:463-470. [DOI:10.1163/187529275X00239]
15. Poinar GO (1990). Taxonomy and Biology of Steinernematidae and Heterorhabditidae. Entomopathogenic Nematodes in Biological Control. Marcel Dekker, New York, USA Pp:23-61.
16. Stock SP, Goodrich-Blair H (2012). Nematode parasites, pathogens and associates of insects and invertebrates of economic importance. Manual of Techniques in Insect Pathology. Academic Press, San Diago. Pp.373-426. [DOI:10.1016/B978-0-12-386899-2.00012-9]
17. White GF (1927). A method for obtaining nematode infected larvae from cultures. Science 66:302-303. [DOI:10.1126/science.66.1709.302.b] [PMID]
18. Wouts WM (1980). Biology, life cycle and redescription of Neoaplectana bibionis Bovien, 1937 (Nematoda: Sternematidae). Journal of Nematology 12:62-71.

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