Volume 3, Issue 1 ((Spring and Summer) 2016)                   Iranian J. Seed Res. 2016, 3(1): 99-107 | Back to browse issues page

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Sadeghi H, Delaviz M, Pirasteh-Anosheh H, Ranjbar G. Comparing the Effect of Seed Pre-Treatment Methods on Improving Alkali Stress Tolerance of Sainfoin and its Recovery. Iranian J. Seed Res.. 2016; 3 (1) :99-107
URL: http://yujs.yu.ac.ir/jisr/article-1-132-en.html
Professor, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran , : h.pirasteh@areeo.ac.ir
Abstract:   (35667 Views)

Germination is the first and the most stages for sainfoin (Onobrychis viciifolia) growth; which it has especially sensitivity to environmental stresses such as alkalinity. The current study was conducted to evaluate the effectiveness of seed pre-treatment methods on improving sainfoin tolerance to alkali stress in germination, early growth and its recovery as a factorial experiment based on a completely randomized design in College of Agriculture, Shiraz University in 2013. The first factor was four seed pre-treatment methods (seed with the pod, seed without the pod, pre-chilling of seed without pod and hydro-priming of seed without pod) and the second factor was five alkali stress levels (pH= 6.7, 7.9, 8.9, 9.8 and 10.7). Alkali stress levels were prepared using two neutral salts (Na₂SO4 and NaCl) and two alkaline salts (NaHCO3 and Na2CO3). The results showed that germination percentage was decreased as alkalinity (pH) levels were enhanced; so that, there were no germinated seeds in 9.8 and 10.7 treatments. Alkali stress reduced initial germination percentage (51.9%), final germination percentage (51.8%), plumule length (55.7%) and radicle length (72.2%). Under all alkalinity conditions, the lowest seed germination and seedling growth were observed in with pod seed, followed by pre-chilling treatments; while their highest were achieved in without pod seeds and hydro-priming treatments. The highest recovery was observed in without the pod, followed by hydro-primed seeds. Recovery was observed in Pre-chilled and with pod speeds up to 7.9 and in hydro-primed and without pod speeds up to 8.9.

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Type of Study: Research | Subject: Seed Physiology
Received: 2015/06/1 | Accepted: 2016/01/24

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