Volume 12, Issue 1 ((Spring and Summer) 2025)
Iranian J. Seed Res. 2025, 12(1): 157-174 |
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Keshavarz H, Jafari Haghighi B, Jafari A, Miri H, Ebrahimi H. (2025). Interaction of Seed Priming, Sulfur, and Thiobacillus on Yield and Fatty Acid Profile of Sesame (Sesamum indicum) Seeds. Iranian J. Seed Res.. 12(1), 157-174.
URL: http://yujs.yu.ac.ir/jisr/article-1-650-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-650-en.html
Arsanjan Branch, Islamic Azad University , barmakjafarihaghighi@iauc.ir
Abstract: (197 Views)
Objective: This study was conducted to investigate the effect of seed priming, sulfur application, and a biofertilizer containing Thiobacillus on seed yield and the fatty acid composition of sesame. Due to the importance of sesame as a valuable oilseed crop, evaluating the combined role of nutritional and biological management in improving both quantitative and qualitative traits of the crop holds significant importance.
Method: A field experiment was conducted during the 2023–2024 growing season in Fasa County, Fars Province, Iran, as a factorial arrangement in a randomized complete block design with three replications. Treatments included four seed priming methods (no prime, hydropriming, calcium chloride at 2.5% and 5%), four levels of sulfur application (0, 100, 200, and 300 kg ha-1), and three levels of Thiobacillus inoculation (0, 2, and 4 kg ha-1). Sesame cultivar ‘Darab 2’ was sown at a density of 40 plants m-2. Thousand-seed weight, dry matter and seed yield were measured. Oil quality was evaluated through fatty acid methyl ester analysis using gas chromatography according to ISIRI and AOAC standards.
Results: Seed priming, sulfur application, and Thiobacillus biofertilization significantly affected all the studied traits. Both two-way and three-way interactions were significant at 1% and 5% levels. The highest plant dry weight (23,580 kg ha-1) was observed in the treatment of hydro-priming combined with 300 kg ha-1 sulfur. Additionally, the highest thousand seed weight (5.33 g) and seed yield (2610 kg ha-1) were recorded in the treatment of hydro-priming combined with 300 kg ha-1 sulfur and 4 kg ha-1 Thiobacillus. The highest oil percentage (52.2%) and protein content (25.10%) were obtained in the treatment of 5% calcium chloride priming combined with 300 kg ha-1 sulfur and 4 kg ha-1 Thiobacillus. The fatty acid analysis revealed that integrated treatments increased the content of unsaturated fatty acids (linoleic, oleic, alpha-linolenic) while decreasing saturated fatty acids (palmitic and stearic).
Conclusions: The integrated use of seed priming (especially 5% calcium chloride or hydropriming), high-dose sulfur, and Thiobacillus inoculation significantly improved sesame seed yield, and oil quality. These practices enhanced nutrient uptake, stimulated microbial activity, and promoted biosynthesis of health-beneficial fatty acids.
Highlights
Method: A field experiment was conducted during the 2023–2024 growing season in Fasa County, Fars Province, Iran, as a factorial arrangement in a randomized complete block design with three replications. Treatments included four seed priming methods (no prime, hydropriming, calcium chloride at 2.5% and 5%), four levels of sulfur application (0, 100, 200, and 300 kg ha-1), and three levels of Thiobacillus inoculation (0, 2, and 4 kg ha-1). Sesame cultivar ‘Darab 2’ was sown at a density of 40 plants m-2. Thousand-seed weight, dry matter and seed yield were measured. Oil quality was evaluated through fatty acid methyl ester analysis using gas chromatography according to ISIRI and AOAC standards.
Results: Seed priming, sulfur application, and Thiobacillus biofertilization significantly affected all the studied traits. Both two-way and three-way interactions were significant at 1% and 5% levels. The highest plant dry weight (23,580 kg ha-1) was observed in the treatment of hydro-priming combined with 300 kg ha-1 sulfur. Additionally, the highest thousand seed weight (5.33 g) and seed yield (2610 kg ha-1) were recorded in the treatment of hydro-priming combined with 300 kg ha-1 sulfur and 4 kg ha-1 Thiobacillus. The highest oil percentage (52.2%) and protein content (25.10%) were obtained in the treatment of 5% calcium chloride priming combined with 300 kg ha-1 sulfur and 4 kg ha-1 Thiobacillus. The fatty acid analysis revealed that integrated treatments increased the content of unsaturated fatty acids (linoleic, oleic, alpha-linolenic) while decreasing saturated fatty acids (palmitic and stearic).
Conclusions: The integrated use of seed priming (especially 5% calcium chloride or hydropriming), high-dose sulfur, and Thiobacillus inoculation significantly improved sesame seed yield, and oil quality. These practices enhanced nutrient uptake, stimulated microbial activity, and promoted biosynthesis of health-beneficial fatty acids.
Highlights
- Seed priming, sulfur application, and Thiobacillus inoculation significantly increased sesame seed yield.
- Combined treatments improved seed oil quality by increasing unsaturated fatty acids and reducing saturated ones.
Type of Study: Research |
Subject:
General
Received: 2025/09/6 | Revised: 2025/09/16 | Accepted: 2025/09/17 | ePublished: 2026/03/18
Received: 2025/09/6 | Revised: 2025/09/16 | Accepted: 2025/09/17 | ePublished: 2026/03/18
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