Volume 7, Issue 2 ((Autumn & Winter) 2021)                   Iranian J. Seed Res. 2021, 7(2): 191-207 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ababaf M, Omidi H, Bakhshandeh A. Determination of Optimum Concentration and Time of Pre-Treatment with Plant Growth Regulators on Germination Indices of Catharanthus roseus Seed. Iranian J. Seed Res.. 2021; 7 (2) :191-207
URL: http://yujs.yu.ac.ir/jisr/article-1-448-en.html
Shahed University , omidi@shahed.ac.ir
Abstract:   (350 Views)
Extended Abstract
Introduction: Catharanthus roseus is regarded as a medicinal ornamental plant. This plant has anti-cancer, anti-hypertensive, anti-diabetes, and antimicrobial properties. Catharanthus has a fairly long vegetative period due to its slow initial growth. The long growth period of the plant is considered one of the limitations in its cultivation on a larger scale. By using plant growth regulators such as salicylic acid (SA) in the imbibition phase and pre-treatment, an increase in seed activity and the seedling growth of many crops is observable. Also, jasmonic acid (JA) plant growth regulators play an important role in seed germination and plant growth. Regarding the long growth period of this plant, the small size of the seed, and poor establishment in the field with semi-heavy and heavy textured soils, the present study aimed to evaluate the effect of seed priming with SA and JA in different concentrations and periods on improving Catharanthus roseus seed germination indices.
Materials and Methods: The studies were conducted as a factorial experiment based on a completely randomized design with three replications at the Laboratory of Seed Science and Technology of Shahed University, Tehran, in 2017. Treatments included five different concentrations of SA (0, 0.01, 0.1, 0.5, and 1 mM), concentrations of JA (0, 1, 10, and 100 µM) and five periods of time (0, 6, 12, 24, and 48 hours). At the end of the experiment (10 days) traits such as germination percentage, germination rate, mean germination time, mean daily germination, germination value, seed length vigor index, seed weight vigor index, seedling dry weight, shoot dry weight, radicle dry weight, radicle length, shoot length, and seedling length were measured.
Results: The results indicated that the effect SA, JA, and time were significant on germination percentage, germination speed, mean germination time, mean daily germination, germination value, seedling length, seedling length vigor index, seedling weight vigor index, and radicle dry weight. The best times for pre-treatment with SA were 24 and 48 hours. Among the applied concentrations of SA, 0.5 and 1 mM concentrations showed the best results. Also, the best time and concentration for pre-treatment with JA were 12 and 24 hours and 10 µM.
Conclusions: The results of this study showed that using SA and JA for seed priming improved seed germination components. In addition to the pre-treatment concentrations of SA and JA, the duration of seed contact with growth regulators is important. It was observed that there was a significant difference among the different priming times, therefore, it can be stated that seed pre-treatment time is one of the important factors of seed priming, and the determination of proper priming time prevented the negative effect of pretreatment on germination and seedling growth in primed seeds.

1- The optimum concentrations of salicylic acid and the priming time of the seed were determined.
2- The appropriate concentrations of jasmonic acid and the optimum time for pretreatment of seed were determined.
3- The effect of growth regulators of salicylic acid and jasmonic acid on seed germination indices was evaluated.
Full-Text [PDF 400 kb]   (79 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2019/11/1 | Accepted: 2020/05/2

1. Abdul-Baki, A.A. and Anderson, J.D. 1973. Vigour determination in soybean by multiple criteria 1. Crop Science, 13(6): 630-633. [DOI:10.2135/cropsci1973.0011183X001300060013x]
2. Ansari, O. and Sharif-Zadeh. F. 2012. Does Gibberellic acid (GA), Salicylic acid (SA) and Ascorbic acid (ASc) improve mountain Rye (Secale montanum) seeds germination and seedlings growth under cold stress? International Research Journal of Applied and Basic Sciences, 3(8): 1651-1657.
3. Ansari, O., Tavakkol Afshari, R., Sharif-Zadeh, F. and Shayanfar. A. 2013. The role of priming on seed reserve utilization and germination of mountain rye (Secale montanum) seeds under salinity stress. Iranian Journal Field Crop Science, 44(2): 181-189. [In Persian with English Summary].
4. Arbaoui, M., Yahia, N. and Belkhodja, M. 2015. Germination of the tomato (Lycopersicon esculentum Mill.) in response to salt stress combined with hormones. International Journal of Agronomy and Agricultural Research, 7(3): 14-24.
5. Aslam, J., Mujib, A., Fatima, S. and Sharma, M. 2008. Cultural conditions affect somatic embryogenesis in Catharanthus roseus L. (G.) Don. Plant Biotechnology Reports, 2(3): 179-189. [DOI:10.1007/s11816-008-0060-9]
6. Azarnia, M., Biabani, A., Eisvand, H.R., Gholamalipour, E. and Safikhani. S. 2016. Effect of seed priming with gibberellic acid and salicylic acid on germination characteristic and seed and seedlings physiological quality of Lentil (Lens culinaris L.). Iranian Journal of Seed Research, 3(1): 59-73. [In Persian with English Summary].
7. Babaei Qaqlstany, A., Asadi Gakiyeh, M., Fallahi, N. and Hatami Ghare Ghoyni. N. 2016. Osmopriming effect on germination of wheat under allelopathic conditions of foxtail extract. Seed Research, 6(18): 11-19. [In Persian with English Summary].
8. Bandurska, H., Stroiński, A. and Kubiś, J. 2003. The effect of jasmonic acid on the accumulation of ABA, proline and spermidine and its influence on membrane injury under water deficit in two barley genotypes. Acta Physiologiae Plantarum, 25(3): 279-285. [DOI:10.1007/s11738-003-0009-0]
9. Basra, S. M., Ullah, E., Warraich, E., Cheema, M., and Afzal, I. 2003. Effect of storage on growth and yield of primed canola (Brassica napus) seeds. International Journal of Agriculture and Biology, 5(2): 117-120.
10. Brancalion, P., Novembre, A., Rodrigues, R. and Tay, D. 2008. Priming of Mimosa bimucronata seeds-a tropical tree species from Brazil. Acta Horticulturae, 782: 163-168. [DOI:10.17660/ActaHortic.2008.782.18]
11. Creelman, R. and Mullet, J.E. 1997. Biosynthesis and action of jasmonate in plant. Annual Review of Plant Physiology and Plant Molecular Biology, 48(1): 355-381. [DOI:10.1146/annurev.arplant.48.1.355] [PMID]
12. Ellis, R.H. and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9: 377-409.
13. Ghasemi Golezani, K. and Dalil, B. 2011.Germination and Seed Vigor Tests. Ferdowsi University of Mashhad. P.104
14. Ghasemi Jobshahr, E., and Khoramivafa. M. 2013. Effect of pretreatment of salicylic acid on germination and seedling properties Callendulla officinalis in salt stress condition. Journal of Plant Production Technology, 12(2): 57-70. [In Persian with English Summary].
15. Harris, D., Pathan, A., Gothkar, P., Joshi, A., Chivasa, W., and Nyamudeza, P. 2001. On-farm seed priming: using participatory methods to revive and refine a key technology. Agricultural Systems, 69(1-2): 151-164. [DOI:10.1016/S0308-521X(01)00023-3]
16. Hoogenboom, G., Huck, M., and Peterson, C. M. 1987. Root growth rate of soybean as affected by drought stress. Agronomy Journal, 79(4): 607-614. https://doi.org/10.2134/agronj1987.00021962007900040003x [DOI:10.2134/agronj1987.00021962007900040004x]
17. Huang, C., Wang, D., Sun, L. and Wei, L. 2016. Effects of exogenous salicylic acid on the physiological characteristics of Dendrobium officinale under chilling stress. Plant Growth Regulation, 79(2): 199-208. [DOI:10.1007/s10725-015-0125-z]
18. Hubbard, M., Germida, J. and Vujanovic, V. 2012. Fungal endophytes improve wheat seed germination under heat and drought stress. Botany, 90(2): 137-149. [DOI:10.1139/b11-091]
19. Ibrahim, E.A. 2016. Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology, 192: 38-46. [DOI:10.1016/j.jplph.2015.12.011] [PMID]
20. Iqbal, M. and Ashraf, M. 2007. Seed treatment with auxins modulates growth and ion partitioning in salt‐stressed wheat plants. Journal of Integrative Plant Biology, 49(7): 1003-1015. [DOI:10.1111/j.1672-9072.2007.00488.x]
21. Kabiri, R., Hatami, A. and Naghizadeh. M. 2014. Effect of drought stress and its interaction with salicylic acid on fennel (Foeniculum vulgare Mill.) germination and early seedling growth. Journal of Medicinal Plants and By-Products, 2: 107-116.
22. Khoramdel, S., Rezvani Moghadam, P., Amin Ghafari, A. and Shabahang. J. 2012. Study the germination characteristics of black seed (Nigella sativa L.) under drought stress condition in different salicylic acid levels. Iranian Journal of Field Crops Research, 10(4): 709-725.
23. Kumari, N., Rai, P.K., Bara, B.M. and Singh. I. 2017. Effect of halo priming and hormonal priming on seed germination and seedling vigor in maize (Zea mays L.) seeds. Journal of Pharmacognosy and Phytochemistry, 6(4): 27-30.
24. Kuźniak, E. and Urbanek, H. 2000. The involvement of hydrogen peroxide in plant responses to stresses. Acta Physiologiae Plantarum, 22(2):195-203. [DOI:10.1007/s11738-000-0076-4]
25. Moghbeli, T. and Arvin, J. 2013. Effect of seed preparation with growth regulators on germination, growth and yield characteristics of (Cucumis melo L.). Journal of Crop Production and Processing, 4: 23-33.
26. Nawaz, A., Sheteiwy, M.S., Khan, S.M., Bukhari, S.A.H., Dawood, M., Guan, Y. and Hu, J. 2017. Exploring the mechanism of exogenous applied methyl jasmonate for germination inhibition in hybrid rice. Pakistan Journal of Life & Social Sciences, 15(1): 60-71.
27. Nejat, N., Valdiani, A., Cahill, D., Tan, Y.H., Maziah, M. and Abiri, R. 2015. Ornamental exterior versus therapeutic interior of Madagascar periwinkle (Catharanthus roseus): the two faces of a versatile herb. The Scientific World Journal, 2015: 1-19. [DOI:10.1155/2015/982412] [PMID] [PMCID]
28. Norastehnia, A., Sajedi, R. & Nojavan-Asghari, M. 2007. Inhibitory effects of methyl jasmonate on seed germination in maize (Zea mays): effect on α-amylase activity and ethylene production. General and Applied Plant Physiology, 33: 13-23.
29. Omidbeigi, R. 2006. Production and Processing of Medicinal Plants. Astane Ghodse Razaviee Publication. 419p. [In Persian].
30. Pagter, M., Bragato, C., Malagoli M. and Brix, H. 2009. Osmotic and ionic effects of NaCl and Na2SO4 salinity on Phragmites australis. Aquatic Botany, 90(1): 43-51. [DOI:10.1016/j.aquabot.2008.05.005]
31. Paparella, S., Araújo, S., Rossi, G., Wijayasinghe, M., Carbonera, D. and Balestrazzi, A. 2015. Seed priming: state of the art and new perspectives. Plant Cell Reports, 34(8): 1281-1293. [DOI:10.1007/s00299-015-1784-y] [PMID]
32. Paravr, A., Omidi, H., Esanejad, N. and Amirzadeh, M. 2015. Effect hydropriming seed germination and seedling growth coneflower (Echinaceac prupurea) under salt stress. Journal Seed Ecophysiology, 1(1): 57-69. [in Persian with English Summary].
33. Rivas-San Vicente, M. and Plasencia, J. 2011. Salicylic acid beyond defence: its role in plant growth and development. Journal of Experimental Botany, 62(10): 3321-3338. [DOI:10.1093/jxb/err031] [PMID]
34. Santini, B.A. and Martorell, C. 2013. Does retained seed priming drive the evolution of serotiny in drylands? An assessment using the cactus Mammillaria hernandezii. American Journal of Botany, 100(2): 365-373. [DOI:10.3732/ajb.1200106] [PMID]
35. Shakirova, F.M., Sakhabutdinova, A.R., Bezrukova, M.V., Fatkhutdinova, R.A. and Fatkhutdinova, D.R. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science, 164(3): 317-322. [DOI:10.1016/S0168-9452(02)00415-6]
36. Taha, H., El-Bahr, M. and Seif-El-Nasr, M. 2009. In vitro studies on Egyptian Catharanthus roseus (L.) G. Don. IV: manipulation of some amino acids as precursors for enhanced of indole alkaloids production in suspension cultures. Australian Journal of Basic and Applied Sciences, 3(4): 3137-3144.
37. Vazirimehr, M., Ganjali, H., Rigi, K. and Keshtehgar, A. 2014. Effect of seed priming on quantitative traits corn. International Journal of Plant, Animal and Environmental Sciences, 4(2):134-140.
38. Zamani, N., and Roshandel, P. 2018. The effect of jasmonic acid on seed germination of halophytes species in high levels of salinity. Iranian Journal of Seed Science and Technology, 7(2): 29-42. [In Persian, with English Summary].
39. Zarei, B., Taghipour, Z. and Fazeli, A. 2016. The effect of seed priming with different concentrations of jasmonic acid on germination and early growth of (Nigella sativa L.) seedling under salinity stress. Journal of Seed Research, 6(2): 67-76. [In Persian, with English Summary].

Add your comments about this article : Your username or Email:

Send email to the article author

© 2021 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.