Meisam Taghinasab, Ebrahim Karimi,
Volume 1, Issue 2 (9-2012)
Abstract
The soft rot causal bacteria, species of Pectobacterium and Dickeya, are important pathogens of plants, which have worldwide distribution. These bacteria are very broad host range and cause plant tissues rot . Diseases caused by these bacteria in the form of soft rot, black stem, bulb rot and leaf spot of corn, rice, canola, sugar beet, potatoes, banana, dates, carrots, cabbage, onions, turnips, peppers, philodendron, pandanus, dieffenbachia, Cyclamen, Iris, Aglaonema, Dracaena, Gladiolus, Ficus, ornamental cactus, and Orobanche have been reported from Iran. Some varieties of field crops such as corn, tomatoes, potatoes and sugar beet, as tolerant to these bacteria in Iran.
Hossein Pasalari,
Volume 10, Issue 1 (2-2021)
Abstract
Pasalari H (2021) The relationship between potato resistance to bacterial soft rot and expression of three PR genes. Plant Pathology Science 10(1):76-85. Doi: 10.2982/PPS.10.1.76.
Introduction: Changes in the resistance to bacterial soft rot in potatoes can be linked to the expression of pathogenesis-related (PR) genes. The aim of this study was to investigate the relationship between the accumulation of PR genes and the induction of resistance through infection of potato tuber cells with pathogenic bacteria at different temperatures in order to effectively combat bacterial soft rot disease in potatoes. Materials and Methods: Pectobacterium carotovorum 2A, Pectobacterium atrosepticum 36A, and Dickeya dadantii ENA49 were used in this study. For bacterial infection, the potato cultivars semi-resistant cultivar Scarab and susceptible cultivar Vesnianka, were used. The factorial experiment with three replications was carried out according to a completely randomized design. The relative level of mRNA copies of PR genes was determined by RT-PCR using primers of these genes. The mean values were compared according to the LSD test. Results: The experiments demonstrated the induction of PR-3, PR-5t and PR-10 in potato tuber cells in response to infection with P. carotovorum 2A, P. atrosepticum 36A and D. dadantii ENA49. It has been shown that the degree of induction of resistance genes depends on the temperature and the potato cultivar. Conclusion: It can be concluded that significant changes in potato resistance to bacterial soft rot at temperatures of 28 and 33 ° C are associated with the expression of these PR genes.
Zahereh Panahi, Reza Khakvar, Naser Aliasgharzad, Saeed Zehtab, Reza Farshabf Pourabad,
Volume 12, Issue 1 (3-2023)
Abstract
Panahi, Z., Khakvar, R., Aliasgharzad, N., Zehtab, S., & Farshbaf PourAbad, R. (2023). The effect of copper nanoparticles on soft rot agent of potato, carrot and onion. Plant Pathology Science, 12(1), 1-11.
Introduction: Bacterial soft rot caused by Pectobacterium species is one of the important and common diseases in Potatoes and vegetables. Disinfection of tubers or seeds with chemicals is one of the methods of disease management. Copper nanoparticles, like silver and gold nanoparticles, have a strong inhibitory effect on bacterial cells, but they are much cheaper and more accessible than them. This research was conducted to determine the effect of copper nanoparticles alone and in combination with oxytetracycline and streptomycin antibiotics on potato, carrot and onion soft rot. Materials and Methods: The pathogen was isolated from rotten tissues of potato, carrot and onion, purified and identified by studying the phenotypic and genetic characteristics of the Pel-gene region using Pectobacterium specific primers (Y1 and Y2). The effect of copper nanoparticles, oxytetracycline and streptomycin, and their combination with copper nanoparticles on the pathogen growth was investigated in a completely randomized design experiment with three replications for each treatment in vitro. Results: Pectobacterium odoriferum was identified as pathogen based on phenotypic characteristics and genetic affinity. Streptomycin compared to oxytetracycline inhibited the pathogen growth more and their effect in combination with copper nanoparticles increased by 12 and 19.5%, respectively. Conclusion: Copper nanoparticles can inhibit the growth of P. odoriferum, and in combination with antibiotics increase their effect.
