Search published articles

Showing 2 results for Heydarnejad

Zohreh Davoodi, Jahangir Heydarnejad, Hossein Masoomi,
Volume 8, Issue 2 (9-2019)

Davoodi Z, Heidarnejad J and Masoumi H (2019) Next generation sequencing technique and its application in plant virology. Plant Pathology Science 8(2):77-85. DOI:10.2982/PPS.8.2.77

DNA sequencing is used by virtually all branches of biological research. Among the first advanced sequencing technologies, scientists were able to elucidate genetic information from any particular biological system using the Sanger sequencing method. Although Sanger sequencing generates high quality sequencing data, its limitations such as scalability, speed and resolution often preclude scientists from obtaining the essential information. To overcome these barriers, next generation sequencing technique (NGS) was introduced at the beginning of the 21st century. This technique provided a highly efficient, rapid, and low cost DNA sequencing platform beyond the reach of the standard and traditional DNA sequencing technologies that developed in late 1970s. In 2009, NGS technologies began to be applied to several areas of plant virology including virus/viroid genome sequencing, discovery and detection, ecology, epidemiology and replication. It is expected that NGS plays very significant roles in many plant virology researches.

Seyyed Ziya Mirhosseini Chahooki, Hossein Massumi, Jahangir Heydarnejad, Akbar Hosseinipour, ‪roohollah Abdolshahi, Mohammad Maddahian,
Volume 11, Issue 1 ((Autumn & Winter) 2022)

Mirhosseini-Chahooki SZ, Massumi H, Heydarnejad J, Hosseinipour A, Abdoshahi R, Maddahian M (2022) The reaction of forty cucurbits genotypes against  Cucumber mosaic virus. Plant Pathology Science 11(1):48-59.
    Doi: 10.2982/PPS.11.1.48.
Introduction: Cucumber mosaic virus (CMV) is one of the most common pathogens of cucurbits plants in the world. The best way to manage the disease is to identify and cultivate resistant cultivars. Materials and Methods: Reaction of 40 genotypes or cultivars of cucurbits plants including Cantaloupe, cucumber, melon, squash and watermelon were evaluated for a CMV isolate from subgroup IB under greenhouse conditions in a completely randomized design with 10 replications per treatment. The plants were inoculated with CMV-infected tobacco extract at the cotyledon leaf stage. Up to four weeks after inoculation, their reaction was determined based on symptom severity index and absorption index in ELISA test (OD405). Results: Genotypes were classified into five groups: highly sensitive, sensitive, relatively resistant, resistant and completely resistant. Conclusion: Cultivation of completely resistant or resistant genotypes for disease management can be recommended.

Page 1 from 1     

© 2023 CC BY-NC 4.0 | University of Yasouj Plant Pathology Science

Designed & Developed by : Yektaweb