Athar Alishiri, Farshad Rakhshandehroo,
Volume 3, Issue 1 (3-2014)
Abstract
Salicylic acid is a kind of phenolic acids and a derivate of salicylate hormone that produced in plants in response to the large numbers of plant pathogens and is necessary for inducing systemic acquired resistance. Vast numbers of studies have done by researchers on the salicylic acid function in inducing plant resistance genes specially those that are responsible for pathogenesis related protein encoding. Results indicated to the effective role of this hormone in inducing innate resistance against pathogenic agents in plants. Changes that block the salicylic acid production in plants increase the disease susceptibility to plant pathogens. Transcription factors belonging to the WRKY, TGA and MYB families are involve in salicylic acid dependent resistance to plant pathogenic agents. Salicylic acid induces the pathogenesis related proteins through the NPR1 dependent pathway during hypersensitive response and systemic acquired resistance reactions. Signaling pathway, independent from NPR1 is a different form of salicylic acid dependent signal transduction and is require for specific resistance against pathogens. Salicylic can also induce the RNA-Dependent-RNA Polymerase gene expressions and play an important role in RNA silencing. Totally, with having knowledge about the salicylic acid biology and its signal transduction pathways in plants it is possible to increase the crop yields with increasing the resistances potential to stresses.
Mahsa Jahandideh, Sevil Nematollahi, Farshad Rakhshandehroo,
Volume 12, Issue 2 ((Spring and Summer) 2023)
Abstract
Jahandideh M, Nematollahi S, Rakhshandehroo F (2023) The reaction of four pumpkin varieties to cucumber mosaic virus by analyzing the expression of PAL and PR2 genes. Plant Pathology Science 12(2):11- 26.
Introduction: Cucumber mosaic virus (CMV) is one of the most important pathogenic cucurbit viruses. Identifying and growing resistant or tolerant varieties is the best method for disease control. The present study was conducted to investigate the response of four pumpkin varieties to CMV by assessing the expression of genes involved in resistance (PAL, PR2). Materials and Methods: Forty samples of pumpkin leaves with suspected disease were collected from the farms in northwestern Iran and analyzed using the TAS-ELISA test. To study the response of the four pumpkin varieties Asma, Pars, Prof and PS grown in this region, a CMV isolate was inoculated into the plants in a greenhouse experiment after its biological purification. TAS-ELISA and semi-quantitative (Sq) RT-PCR tests were used to examine the virus concentration in pumpkin varieties. The disease severity index was evaluated 30 days after inoculation. The expression level of PAL and PR2 genes was also checked by quantitative real-time PCR technique. Results: Of the 40 samples, 16 samples were infected with CMV. The study of virus titer revealed that the virus concentration and disease severity index were higher in Pars and Proof varieties than in PS and Asma varieties. The expression of PAL and PR2 genes was increased in all varieties compared to control but was higher in PS cultivar followed by Asma. Conclusion: PS and Asma varieties have higher CMV tolerance and their wider cultivation is recommended for disease control.
