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Fariba Ghaderi, Hojatollah Mohammadi,
Volume 12, Issue 2 (9-2023)
Abstract

Ghaderi F, Mohammadi H (2023) Occurrence of jujube brown spot disease in Iran. Plant Pathology Science 12(2):95-104. 
Jujube tree has a natural distribution in tropical and sub-tropical regions of Asia. Symptoms of brown-to-black spots on leaves, and fruits, and twigs blight were observed in the hills of the suburbs of Nurabad County, Fars Province, Iran, in 2022. This research was conducted to identify the cause of this disease based on morphological and genetic characteristics. The diseased leaves and branches of the neighboring trees in this area were sampled. The pathogen was isolated and purified after surface disinfection of disease tissues on potato/dextrose/agar medium. Its morphological characteristics were studied and the fungus Nothophoma quercina was identified. Phylogenetic analysis base on the comparison of beta-tubulin (tub2), and ITS-rDNA genes sequences, with related fungi in NCBI Gen Bank, confirmed the of N. quercina species. Its pathogenicity was proved on the side cut jujube branches based on Koch's postulates in vitro. This is the first report of brown spot and twigs blight of the jujube trees caused by N. quercina in Iran.

Zahra Mirzaeipour, Eidi Bazgir, Doustmorad Zafari, Mostafa Darvishnia,
Volume 12, Issue 2 (9-2023)
Abstract

Mirzaeipour Z, Bazgir E, Zafari D, Darvishnia M (2023) Effect of temperature and culture medium on the growth and sporulation of eight Trichoderma species. Plant Pathology Science 12(2):105-116. DOI: https://doi.org/10.2982/PPS.12.2.105

Trichoderma species are important agents of biological control of soil-borne plant pathogens. The growth and reproduction of these fungi are influenced by the culture medium and temperature. This study was conducted to determine the effect of temperature and culture medium on the growth and sporulation of Trichoderma species. Ten isolates of Trichoderma species were isolated from agricultural soils of different regions of Lorestan Province, Iran. The study of morphological characteristics and sequencing of ITS-rDNA, and tef1α gene regions showed that they are belong to eight species of Trichoderma. Investigating the effect of four types of culture medium and five temperatures to determine optimum culture medium and temperature for the growth and reproduction of these fungi, showed that the Potato/Dextrose/Agar (PDA) medium is the best, and the temperature of 20 to 30 degrees Celsius is optimal for the growth and reproduction of these fungi. Evaluation of their ability to inhibit the growth of the soil-borne plant pathogenic fungus Rhizoctonia solani in vitro, showed that T. harzianum LT8 has the most inhibition ability. Therefore, this isolate can be used as a potential biocontrol agent for this plant pathogenic fungus in future research.
 

Azadeh Goudarzi,
Volume 12, Issue 2 (9-2023)
Abstract

Goudarzi A (2023) Anthracnose disease of citrus. Plant Pathology Science 12(2):117-129.
Colletotrichum species are the causal agents of diseases including leaf spots, fruit rots and dieback in a wide range of plant hosts such as citrus, banana, strawberry, avocado, papaya, mango and apple. In citrus, important diseases including anthracnose, post harvest anthracnose, key lime anthracnose and postbloom fruit drop are caused by different species of Colletotrichum which are of the major limitations of citrus production in many citrus producing countries worldwide. Colletotrichum gloeosporioides has been identified as the main cause of citrus anthracnose. The disease symptoms include dieback of branches and twigs, leaf spot, fruit rot and abscission of leaves and small fruits. In the case of epidemic occurrence, the damage caused by the disease on different species and cultivars of citrus is very significant. The economic losses caused by the disease is mainly related to damage to the vegetative parts of citrus trees, as well as decay and reduction of market value of the fruits. So far, citrus anthracnose and postharvest anthracnose caused by C. gloeosporioides, C. fructicola, C. siamense, C. karstii and C. novae-zelandiae have been reported from Iran. In addition, dieback and postbloom fruit drop caused by C. gloeosporioides are of the common challenges of citrus production in the north of the country. Sanitation, management of irrigation and nutrition, control of pests, diseases and weeds and use of chemical fungicides, especially strobilurins, are effective approaches of the disease management. In order to prevent the emergence of resistant populations of the causal agents, the use of fungicides should be carried out with periodicity.


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