Deficiency of soil nutrient elements is one of the most important restricting factors of crop production. Hence, management of nutrition is necessary for optimizing of plant growth, yield increment and sustainability of crop production. In order to evaluate growth stages and some quantitative indices in winter rapeseed (Brassica napus L.) cultivars as affected by micronutrient fertilizers, a field experiment was performed in 2010-2011 cropping seasons as a split plot arranged in randomized complete block design with three replications in Arak, Iran. Eight micronutrient fertilizers (Zero as control, Fe, Zn, Mn, Fe + Zn, Fe + Mn, Zn + Mn, Fe + Zn + Mn) and four rapeseed cultivars (Zarfam, Okapi, Modena and Licord) were randomized in main plots and subplots, respectively. The result showed that, Okapi cultivar had the highest grain yield under no fertilization treatment (4194 kg/ha) and Zn + Mn (4011 kg/ha) treatments, respectively. Licord cultivar produced the highest grain yield (3998 kg/ha) at Mn treatment, but there were no significant differences between fertilizer levels. Okapi variety showed the highest biological yield as affected by Fe + Zn (13666 kg/ha), Zn + Mn (13221 kg/ha) and control (12944 kg/ha) treatments, respectively. In this experiment, the interaction between Licord Mn, Licord (Fe + Zn + Mn) and Okapi (Fe + Zn + Mn) had the highest harvest index (35.46, 34.77 and 32.23 percent), respectively. In addition, the interaction between Licord (Fe + Zn + Mn) showed the highest silique number (369.86) per plant. In general, seed yield and its component in rapeseed varieties showed different responses to micronutrient fertilizers. According to the results of this experiment, cultivation of Okapi and Licord varieties and application of Mn and Mn + Zn fertilizers cased to enhance seed yield of canola in Arak climatic conditions.