Volume 9, Issue 2 ((Autumn & Winter) 2023)                   Iranian J. Seed Res. 2023, 9(2): 51-62 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Fazeli-Nasab B, Khajeh H, Piri R, Moradian Z. (2023). Effect of humic acid on germination characteristics of Lallemantia royleana and Cyamopsis tetragonoloba under salinity stress. Iranian J. Seed Res.. 9(2), 51-62.
URL: http://yujs.yu.ac.ir/jisr/article-1-541-en.html
University of Zabol , bfazeli@uoz.ac.ir
Abstract:   (736 Views)
Extended Abstract
Introduction: Lallemantia royleana is an annual herbaceous plant of Lamiaceae family in different parts of Europe, the Middle East, and especially Iran. Cyamopsis tetragonoloba L. is a plant of the legume family. A common feature between these two plants is hydrocolloid gums, which stabilize some food emulsions by absorbing water and increasing the viscosity or forming a gel in the aqueous phase. Due to its diverse and rich vegetation, Iran can produce countless types of plant gums, and many seeds such as Lallemantia royleana and Cyamopsis tetragonoloba contain valuable gums. Considering the important therapeutic and industrial applications of C. tetragonoloba and L. royleanaplants and the need for more information and reports on determining the best humic acid level and salt stress tolerance of these plants, the purpose of this research is to investigate the tolerance of two L.  royleana and C. tetragonoloba plants to salinity stress in the germination stage and the initial stages of the growth of two plants under the humic acid application.
Materials and Methods: The experiment was carried out as factorial in a completely randomized design with three replications at the seed laboratory of the Faculty of Agriculture of Zabol University in 1400. In this experiment, salinity stress was investigated using sodium chloride at control (no salinity), 70, 140, 210 mM levels and humic acid at (0, 40, 80, and 120 mg/L) levels. Humic acid solution at different salinity levels was added to each petri dish containing 25 seeds.
Results: The results showed that salinity stress decreased germination percentage, radicle length, plumule length, seedling length and seedling dry weight of C. tetragonoloba. In this plant, the germination percentage decreased by 35.34% compared to the control as the salinity stress level increased to 210 mM, and with the increase of the stress to more than 140 mM, a significant decrease in the germination percentage was observed. The maximum plumule length of L. royleanaplants was obtained in 70 mM salinity treatment and 40 mg/L fertilizer level. The maximum radicle length in the L.  royleanaplants plant was obtained in the treatment of 40 mg/L of humic acid at a 70 mM salinity stress level. Also, the results showed that the maximum radicle length (1.46 cm) in the C.  tetragonoloba plant was related to humic acid pretreatment at 70 and 140 mM salinity and fertilizer levels of 40 and 80 mg/L.
Conclusion: In general, it can be stated that the germination indices significantly decreased under stress conditions, and this indicates that humic acid is a suitable pretreatment that can improve the growth indices of C. tetragonoloba and L.  royleanaplants under stressed and non-stressed conditions. With the application of humic acid at all salinity levels except 210 mM, the germination indices in the two mentioned plants were in a favorable condition.

Highlight:
1- The salinity stress tolerance threshold was studied in Cyamopsis tetragonoloba and Lallemantia plants.
2- Humic acid fertilizer in this study mitigated the destructive effects of salinity stress in Cyamopsis tetragonoloba and Lallemantia plants.
Article number: 4
Full-Text [PDF 425 kb]   (151 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2021/12/17 | Accepted: 2022/06/12

References
1. Ahmadi, R. and Maleki Farahani, S. 2021. Effects of sowing date and nitrogen fertilizer on quantitative and qualitative characteristics, and nitrogen efficiency in Lallemantia iberica (M. Bieb.) fisch. & ca mey. and Lallemantia royleana (benth.) benth. Iranian Journal of Medicinal and Aromatic Plants Research, 37(1): 65-82. [In Persian with English Summary].
2. Alizadeh, M. and Isavand, H. 2004. Evaluation and the study of germination potential, speed of germination and vigor index of the seeds of two species of medicinal plants (Eruca sativa Lam., Anthemis altissima L.) under cold room and dry storage condition. Iranian Journal of Medicinal and Aromatic Plants, 20(3): 301-307. [In Persian with English Summary].
3. Amiri, M.B., Rezvani Moghaddam, P., Ehyai, H.R., Fallahi, J. and Aghhavani Shajari, M. 2011. Effect of osmotic and salinity stresses on germination and seedling growth indices of artichoke (Cynara scoolymus) and purple coneflower (Echinacea purpurea). Environmental Stresses in Crop Sciences, 3(2): 165-176. [In Persian with English Summary].
4. Asgharipour, M. and Rafiei, M. 2011. The effect of different concentrations of humic acid on seed germination behavior and vigor of barley. Australian Journal of Basic and Applied Sciences, 5(12): 610-613.
5. Azad, H., Fazeli-Nasab, B. and Sobhanizade, A. 2017. A study into the effect of jasmonic and humic acids on some germination characteristics of rosselle (Hibiscus sabdariffa) seed under salinity stress. Iranian Journal of Seed Research, 4(1): 1-18. [In Persian with English Summary]. [DOI:10.29252/yujs.4.1.1]
6. Bavarsadi, M., Modhej, A. and Majdam, M. 2017. Investigation the effect of salinity tension on germination, seedling growth and ionic content of alfalfa genotypes (Medicago sativa L.). Crop Physiology Journal, 9: 121-136.
7. Çimrin, K.M., Türkmen, Ö., Turan, M. and Tuncer, B. 2010. Phosphorus and humic acid application alleviate salinity stress of pepper seedling. African Journal of Biotechnology, 9(36): 5845-5851.
8. Cordeiro, F.C., Santa-Catarina, C., Silveira, V. and de Souza, S.R. 2011. Humic acid effect on catalase activity and the generation of reactive oxygen species in corn (Zea mays). Bioscience, Biotechnology, and Biochemistry, 75(1): 70-74. [DOI:10.1271/bbb.100553] [PMID]
9. Dursun, A., Güvenç, I. and Turan, M. 2002. Effects of different levels of humic acid on seedling growth and macro and micronutrient contents of tomato and eggplant. Acta Agrobotanica, 55(2): 81-88. [DOI:10.5586/aa.2002.046]
10. Ebrahimi, M. and Miri Karbasak, E. 2016. Investigation effect of humic acid on germination, seedling growth and photosynthesis pigments of medicinal plant isabgol (Plantago ovata forssk). Iranian Journal of Seed Sciences and Research, 3(3): 35-46. [In Persian with English Summary].
11. Ghorbani, S., Khaaje Hoseini, M. and Eishi Rezaei, E. 2013. Effect of pretreatment of humic acid on germination and early seedling growth of maize (Zea mays L.). Iranian Journal of Agronomy and Plant Breeding, 9(3): 37-43. [In Persian with English Summary].
12. Hoseyni, H. and Rezvani Moghadam, P. 2006. Effect of water and salinity stress in seed germination on isabgol (Plantago ovata). Iranian Journal of Field Crops Research, 4(1): 15-22. [In Persian with English Summary].
13. Jamil, M. and Rha, E.-S. 2004. The effect of salinity (NaCl) on the germination and seedling of sugar beet (Beta vulgaris L.) and cabbage (Brassica oleracea L.). Plant Resources, 7(3): 226-232.
14. Keshavarz Afshar, R., Keykhah, M., Chaeichi, M.R. and Ansari, M. 2013. Effect of different levels of salinity and drought stress on seed germination characteristics and seedling growth of forage turnip (Brassica rapa L.). Iranian Journal of Field Crop Science, 43(4): 1-12. [In Persian with English Summary].
15. Khademalhosseini, Z., Jafarian, Z., Roshan, V. and Ranjbar, G. 2018. Effect of water salinity on quantity and quality of biochemical characteristics of Mellissa officinallis L. Journal of Rangeland, 12(3): 370-379. [In Persian with English Summary].
16. Khammari, I., Sarani, S.A. and Dahmardeh, M. 2007. The effect of salinity on seed germination and growth in six medicinal plants. Iranian Journal of Medicinal and Aromatic Plants Research, 23(3): 331-339. [In Persian with English Summary].
17. Khodai Joqan, A., Mushtatti, A., Mousavi, S.H., Pakdaman Sardroud, B. and Abdullahi Nowrozi, M. 2018. Investigating the reduction of the effects of drought stress on the growth and yield of wheat by using zeolite and Piriformospora indica fungus. Water Research in Agriculture (Soil and Water Sciences), 33(4): 613-627. [In Persian with English Summary].
18. Khan, A., Khan, M., Hussain, F., Akhtar, M., Gurmani, A. and Khan, S. 2013. Effect of humic acid on the growth, yield, nutrient composition, photosynthetic pigment and total sugar contents of peas (Pisum sativum L.). Journal of the Chemical Society of Pakistan, 35: 206-211.
19. Khodaei Joghan, Aydin and Zare, Ahmad and Mousavi, Sidamir and Parvin, Laleh, 2018. The effect of time and concentration of humic acid priming on germination of guava seeds (Cyamopsis tetragonoloba L.) under salinity stress, 16th National Congress of Agriculture and Plant Breeding Iran, Ahvaz. [In Persian with English Summary].
20. Orlovsky, N., Japakova, U., Zhang, H. and Volis, S. 2016. Effect of salinity on seed germination, growth and ion content in dimorphic seeds of Salicornia europaea L. (Chenopodiaceae). Plant Diversity, 38(4): 183-189. [DOI:10.1016/j.pld.2016.06.005] [PMID] [PMCID]
21. Ouni, Y., Ghnaya, T., Montemurro, F., Abdelly, C. and Lakhdar, A. 2014. The role of humic substances in mitigating the harmful effects of soil salinity and improve plant productivity. International Journal of Plant Production, 8(3): 353-374.
22. Moradi, A., Hoseinimoghadam, M., and Piri, R. 2018. Effect of seed inoculation with plant growth promoting rhizobactria (pgpr) on some germination, biochemical indices and element contents of fennel (Foeniculum vulgare L.) under salinity stress. Iranian Journal of Field Crop Science, 49(3): 151-165. [In Persian with English Summary].
23. Netondo, G.W., Onyango, J.C. and Beck, E. 2004. Sorghum and salinity: Ii. Gas exchange and chlorophyll fluorescence of sorghum under salt stress. Crop Science, 44(3): 806-811. https://doi.org/10.2135/cropsci2004.8060 https://doi.org/10.2135/cropsci2004.0797 [DOI:10.2135/cropsci2004.0806]
24. Pathak, R. and Roy, M. 2015. Climatic responses, environmental indices and interrelationships between qualitative and quantitative traits in clusterbean Cyamopsis tetragonoloba (L.) taub. Under arid conditions. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 85(1): 147-154. [DOI:10.1007/s40011-013-0269-4]
25. Piri, R., Moradi, A. and Balouchi, H. 2020. Improvement of salinity stress in cumin (Cuminum cyminum) seedling by inoculation with rhizobacteria. Indian Journal of Agricultural Sciences, 90(2): 371-375. [DOI:10.56093/ijas.v90i2.99025]
26. Rajabi Dehnavi, A., Zahedi, M., Ludwiczak, A., Cardenas Perez, S. and Piernik, A. 2020. Effect of salinity on seed germination and seedling development of sorghum (Sorghum bicolor (L.) Moench) genotypes. Agronomy, 10(6): 859. [DOI:10.3390/agronomy10060859]
27. Sofi, A., Ebrahimi, M. and Shirmohammadi, E. 2018. Effect of humic acid on germination, growth, and photosynthetic pigments of Medicago sativa L. Under salt stress. Ecopersia, 6(1): 21-30.
28. Turkmen, Ö., Dursun, A., Turan, M. and Erdinç, Ç. 2004. Calcium and humic acid affect seed germination, growth, and nutrient content of tomato (Lycopersicon esculentum L.) seedlings under saline soil conditions. Acta Agriculturae Scandinavica, Section B-Soil & Plant Science, 54(3): 168-174. [DOI:10.1080/09064710310022014]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb


This work is licensed under a Creative Commons Attribution 4.0 International License.