Volume 11, Issue 1 ((Spring and Summer) 2024)
Iranian J. Seed Res. 2024, 11(1): 43-60 |
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Rezaee Chermehini M, Sharifzadeh F, Sabokdast M. (2024). Enhancement of the seed germination characteristics and longevity of Forssk (Suaeda fruticosa) under salinity stress using hormone-priming and post-priming heat-shock treatments. Iranian J. Seed Res.. 11(1), 43-60.
URL: http://yujs.yu.ac.ir/jisr/article-1-609-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-609-en.html
College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. , sharifz@ut.ac.ir
Abstract: (533 Views)
Extended abstract
Introduction: In order to improve seed germination under environmental stress conditions such as salinity, one of the solutions is to use seed priming. Despite this advantage of priming, the longevity of primed seeds usually decreases and it has been observed that using post-priming treatments such as heat shock can improve the longevity of primed seeds. This research investigates the effect of seed priming with salicylic acid, methyl jasmonate, and brassinosteroid under salinity stress and the effect of heat shock after priming on improving the longevity of primed seeds.
Materials and Methods: The effect of seed priming with hormonal substances such as methyl jasmonate at three concentrations of 1, 100, and 1000 micromolar and brassinosteroid at three concentrations of 25, 75, and 100 mg/liter on increasing the seed germination traits of Suaeda fruticosa was investigated under salinity stress during separate experiments. In both studied hormones, priming temperatures of 5, 10, and 15°C were used for 1 and 2 days. Hormonal seed priming with salicylic acid at a concentration of 25 mg L-1 for 2 days at 10°C -obtained from the previous results (unpublished)- was used for comparison with those of the studied treatments. To determine the most suitable accelerated aging stress for evaluating and determining the best heat shock treatment, the accelerated aging test of seeds was carried out for two, four, six, eight, and ten days. In this research, to improve the longevity of primed seeds, they were exposed to heat shock treatment at temperatures of 30, 35, and 40°C for 1, 2, 3, and 4 hours after seed priming.
Results: Both seed priming treatments with methyl jasmonate and brassinosteroid significantly increased all the tested germination traits compared to the control (without priming). This increase in traits such as germination percentage, vigor index, and seedling length in seed priming with methyl jasmonate was 46.4%, 67%, and 41%, respectively, and in the case of priming with brassinosteroid was 32%, 44%, and 38%, respectively. Also, in this research, the heat shock treatment at 40°C for four hours after priming had a significant and positive effect on seed germination. Application of the mentioned treatment increased germination by 60% at the end of the aging period compared with the control (without the application of heat shock).
Conclusions: Despite the significant increase in germination percentage by each of the hormonal substances like methyl jasmonate and brassinosteroid, the comparison of the best treatment combination of these substances with salicylic acid showed that seed priming with 25 mg L-1 of salicylic acid for two days at 10°C caused a significant increase in the percentage of germination under salinity stress. Also, the heat-shock post-priming treatment significantly improved the longevity of primed seeds.
Highlights:
Introduction: In order to improve seed germination under environmental stress conditions such as salinity, one of the solutions is to use seed priming. Despite this advantage of priming, the longevity of primed seeds usually decreases and it has been observed that using post-priming treatments such as heat shock can improve the longevity of primed seeds. This research investigates the effect of seed priming with salicylic acid, methyl jasmonate, and brassinosteroid under salinity stress and the effect of heat shock after priming on improving the longevity of primed seeds.
Materials and Methods: The effect of seed priming with hormonal substances such as methyl jasmonate at three concentrations of 1, 100, and 1000 micromolar and brassinosteroid at three concentrations of 25, 75, and 100 mg/liter on increasing the seed germination traits of Suaeda fruticosa was investigated under salinity stress during separate experiments. In both studied hormones, priming temperatures of 5, 10, and 15°C were used for 1 and 2 days. Hormonal seed priming with salicylic acid at a concentration of 25 mg L-1 for 2 days at 10°C -obtained from the previous results (unpublished)- was used for comparison with those of the studied treatments. To determine the most suitable accelerated aging stress for evaluating and determining the best heat shock treatment, the accelerated aging test of seeds was carried out for two, four, six, eight, and ten days. In this research, to improve the longevity of primed seeds, they were exposed to heat shock treatment at temperatures of 30, 35, and 40°C for 1, 2, 3, and 4 hours after seed priming.
Results: Both seed priming treatments with methyl jasmonate and brassinosteroid significantly increased all the tested germination traits compared to the control (without priming). This increase in traits such as germination percentage, vigor index, and seedling length in seed priming with methyl jasmonate was 46.4%, 67%, and 41%, respectively, and in the case of priming with brassinosteroid was 32%, 44%, and 38%, respectively. Also, in this research, the heat shock treatment at 40°C for four hours after priming had a significant and positive effect on seed germination. Application of the mentioned treatment increased germination by 60% at the end of the aging period compared with the control (without the application of heat shock).
Conclusions: Despite the significant increase in germination percentage by each of the hormonal substances like methyl jasmonate and brassinosteroid, the comparison of the best treatment combination of these substances with salicylic acid showed that seed priming with 25 mg L-1 of salicylic acid for two days at 10°C caused a significant increase in the percentage of germination under salinity stress. Also, the heat-shock post-priming treatment significantly improved the longevity of primed seeds.
Highlights:
- Seed priming significantly increases the seed germination characteristics of the Forssk plant against high salinity stress.
- As the aging period increases, the primed seeds experience a significant drop in germination compared with the control.
- Heat shock immediately after priming can significantly increase the longevity of primed seeds compared with the control.
Type of Study: Research |
Subject:
Seed Physiology
Received: 2024/04/16 | Revised: 2024/05/25 | Accepted: 2024/05/28 | ePublished: 2024/09/21
Received: 2024/04/16 | Revised: 2024/05/25 | Accepted: 2024/05/28 | ePublished: 2024/09/21
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