Volume 7, Issue 1 ((Spring and Summer) 2020)
Iranian J. Seed Res. 2020, 7(1): 67-82 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Gholamalipour Alamdari E, Ghorbani A, Sabouri H, Habibi M. (2020). Studying the Hetrotoxicity Potential of the Phenolic Composition Obtained from the Methanolic Extract of Echinochola crus-galli Weed on Germination Traits and Cytogenetic Behavior of Rice. Iranian J. Seed Res.. 7(1), : 5 doi:10.29252/yujs.7.1.67
URL: http://yujs.yu.ac.ir/jisr/article-1-428-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-428-en.html
Gonbad Kavous University , eg.alamdari@gmail.com
Abstract: (5196 Views)
Extended abstract
Introduction: Without a doubt, plant hetrotoxicity is one of the important factors in determining the distribution and abundance of some species in plant communities. Thus, the purpose of this experiment was to evaluate the effect of phenolic composition obtained from the methanol extract of Echinochola crus-galli on germination traits and cytogenetic behavior of rice.
Materials and methods: This experiment was done to assess hetrotoxic potential of various concentrations (0, 0.024, 0.048, 0.076 and 0.1 mM) of the phenolic composition obtained from the methanol extract of whole-organ of E. crus-galli on germination traits of rice as well as mitosis division of meristematic cells of radicle in a completely randomized design. To extract thephenolic composition, warm extraction method using a methanol solvent was used. For studying mitosis division, first rice seeds were germinated. Then, each of the steps such as fixation, hydrolysis, staining, squashing and microscopic studies were done on the end of the radicle. Mitosis indices and percentage of mitosis inhibition were calculated and also percentage of each of chromosomal abnormalities at four stages of prophase, metaphase, anaphase and telophase as compared to total cells was calculated.
Results: The lowest percentage and rate of germination and relative germination were found in two concentrations of the 0.076 and 0.1 mM of phenolic composition of E. crus-galli, so that no germination was observed in these treatments. In this study, mitosis division was normal in control samples, so that the rice plant included 12 chromosomes in the metaphase stage. Also the chromosomes were normal in the telophase stage and chromosomal abnormalities were not observed in meristem cells of radicle tip of the control. The lowest value of mitosis indices and the number of dividing cells were related to the concentration of 0.048 mM wuth 30.19 and 385 cells, respectively. In the present study, chromosomal abnormalities in the stages of metaphase, anaphase and telophase were increased with increasing concentration of phenolic composition, and were 28.85 and 16.95% in 0.048 mM concentration of phenolic composition, respectively. The most chromosomal abnormalities were of sticky and laggard type, which were related to the concentration of 0.048 mM of phenolic composition with 39.83 and 32.25%, respectively. The highest number of chromosomal bridges and clumping were obtained in 0.024 mM of phenolic composition with about 19.27 and 29.83%, respectively.
Conclusion: In this study, phenolic composition obtained from the methanol extract of E. crus-galli had asignificant inhibitory effect on germination traits and mitosis division in root tip cells of rice. Thus, the amount of E. crus-galli residues in the field should be considered in direct and indirect cultivation of rice.
Highlights:
1-Difference in impact of the phenolic composition obtained from the methanol extract of Echinochola crus-galli on germination and reduced cytogenetic behavior of rice is related to their threshold concentration.
2- It is advised to cultivate varieties of rice resistant to the remnants of harmful compounds of E. crus-galli as direct cultivation or under nursery condition.
Article number: 5
Keywords: Bridge chromosomes, Chromosomal abnormalities Phenolic composition, Relative germination percentage
Type of Study: Research |
Subject:
Seed Ecology
Received: 2019/07/21 | Revised: 2021/03/13 | Accepted: 2020/01/14 | ePublished: 2020/11/29
Received: 2019/07/21 | Revised: 2021/03/13 | Accepted: 2020/01/14 | ePublished: 2020/11/29
References
1. Abdul-Rauf, K.M. and Siddiqui, M.B. 2012. Allelopathic effect of aqueous extracts of different parts of Tinospora cordifolia (Willd.) Miers on some weed plants. Journal of Agricultural Extension and Rural Development, 4(6): 115-119. [DOI:10.5897/JAERD11.069]
2. Abdul Rauf, K.M. and Siddiqui, M.B. 2013. Allelotoxic effect of parthenin on cytomorphology of broad bean (Vicia faba L.). Journal of the Saudi Society of Agricultural Sciences, 12: 143-146. [DOI:10.1016/j.jssas.2012.11.001]
3. Azadbakht, A., Amraie, R., Mirzapour, S.R. and Nasrollahi, H. 2012. Effect of weed competition on growth characteristics of sunflower at different levels of nitrogen fertilizer. Annals of Biological Research, 3(11): 5162-5168.
4. Badr, A. and Elkington, T.T. 1982. Antimitotic and chromo toxic activities of Isoproturon in Allium cepa and Hordeum vulgare. Environmental and Experimental Botany, 22(3): 265-270. [DOI:10.1016/0098-8472(82)90017-X]
5. Bakhshi Khaniki, Gh.R. 2010. Plant cytogenetic. Payam Noor Publisher. 402p. [In Persian]
6. Caceres, A. 2000. Calidad de la material prima para la elaboracion de productos fitofarma ceuticas. Primer Congreso International FITO 2000 "Por la investigacion, conservacion y diffusion del conocimiento de las plantas medicinal". 27-30 de septiembre, 2000, Lima, Peru.
7. Cai, S.L. and Mu, X.Q. 2012. Allelopathic potential of aqueous leaf extracts of Datura stramonium L. on seed germination, seedling growth and root anatomy of Glycine max (L.) Merrill. Allelopathy Journal, 30: 235-245.
8. Cruz-Ortega, R., Lara-Núñez, A. and Anaya, A.L. 2007. Allelochemical stress can trigger oxidative damage in receptor plants. Plant Signaling and Behavior, 2(4): 269-270. [DOI:10.4161/psb.2.4.3895] [PMID] [PMCID]
9. Dang Khanh, T., Huu Trung, K. and Hoang Anh, L.A. 2018. Allelopathy of Barnyardgrass (Echinochloa crus-galli) Weed: an Allelopathic Interaction with Rice (Oryza sativa). Vietnam Journal of Agricultural Sciences, 1(1): 97-116. [DOI:10.31817/vjas.2018.1.1.10]
10. Davarynejad, G.H., Taghizadeh, S.F. and Asili, J. 2017. Effect of different solvents on total phenolic contents and antioxidant activity of Zizyphus jujube Miller fruits. Journal of Horticulture Science, 21(1): 158-166.
11. Einhellig, F.A. 2002. The physiology of allelochemical action: cluesand views. In: Reigosa, M.J., Pedrol, N. (eds.), Allelopathy, from Molecules to Ecosystems. Science Publishers, Enfield, New Hampshire.
12. El-Shora, H.M. and Abd El- Gawad, A.M. 2015. Physiological and biochemical responses of Cucurbita pepo L. mediated by Portulaca oleracea L. allelopathy. Fresenius Environmental Bulletin, 24: 386-393.
13. Erdman, J.W., Balentine, D., Arab, L., Beecher, G., Dwyer, J.T., Folts, J., Harnly, J., Hollman, P., Keen, C.L., Mazza, G., Messina, M., Scalbert, A., Vita, J., Williamson, G. and Burrowes, J. 2007. Flavonoids and heart health: Proceedings of the ILSI North America flavonoids workshop. Nutrition, 137: 718-737. [DOI:10.1093/jn/137.3.718S] [PMID]
14. Erfani, E.R. 2002. Final report collection and knowledge weeds in rice field and current methods control in Mazandaran. Rice Research Institute of Mazandaran. [In Persian].
15. Fawzia, Mohamed, I. and Zeinab El-Ashry, M. 2012. Cytogenetic effect of allelochemicals Brassica nigra L. extracts on Pisum sativum L. World Applied Sciences Journal, 20(3): 344-353.
16. Gabara, B., Kalwinek, J., KozirÓg, Z., Zakowska, Z. and Brycki, B. 2006. Influence of N, N-bis (3-aminopropylo) dodecyloamine on the ultra-structure of nuclei in Aspergillus niger mycelium and on cell proliferation and mitotic disturbances in Allium cepa L. root meristem. Acta Biologica Cracoviensia, Series Botanica, 48: 45-52.
17. Glin'ska, S., Bartczak, M., Oleksiak, S., Wolska, A., Gabara, B., Posmyk, M. and Janas, K. 2007. Effects of anthocyanin-rich extract from red cabbage leaves on meristematic cells of Allium cepa L. roots treated with heavy metals. Ecotoxicology and Environmental Safety, 68(3): 343-350. [DOI:10.1016/j.ecoenv.2007.02.004] [PMID]
18. Haji Mehdipour, H., Khanavi, M., Shekarchi, M., Abedi, Z. and Pirali Hamedani, M. 2010. The determination of best extracting method of phenolic compounds in Echinacea angustifolia. Quarterly Journal of Medicinal Plants, 8(4): 32: 145-152. [In Persian with English Summary].
19. Hardgree, S.P. and Van Vactor, S.S. 2000. Germination and emergence of primed grass seeds under field and simulated-field temperature regimes. Annals of Botany, 85: 379-390. [DOI:10.1006/anbo.1999.1076]
20. Hasanuzzaman, M., Ali, M. H. Alam, M.M., Akther, M. and Fakhrul Alam, K. 2009. Evaluation of pre-emergence herbicide and hand weeding on the weed control efficiency and performance of transplanted Aus rice. American-Eurasian Journal of Agronomy, 2(3): 138-143.
21. Heidarzade, A., Esmaeili, M. and Pirdashti, H. 2012. Common allelochemicals in root exudates of barnyardgrass (Echinochloa crussgalli L.) and inhibitory potential against rice (Oryza sativa) cultivars. International Research Journal of Applied Basic Science, 3(1): 11-17.
22. Kadiolu, I. and Yanar, Y. 2004. Alelopathic effect of plant extracts against seed germination of some weeds. Asian Journal of Plant Sciences, 3(4): 472-475. [DOI:10.3923/ajps.2004.472.475]
23. Kazemi, M., Roshandel, P. and Rafiei Al- Hosseini, M. 2017. Evaluation of allelopathic potential of six medicinal plants on some physiological and biochemical characteristics of Beta vulgaris and its two important weeds. Journal of Plant Production and Function, 6: 65-80.
24. Khorasani Nezhad, S., Galeshi, A.K. and Fakhr Tabatabaie, S.M. 2009. Study of allelopathic potential of various organ extract of cucumber on seedling growth indices of cucumber, tomato, pepper and basil. Journal of Iranian Horticultural Sciences, 40(1): 55-60. [In Persian with English Summary].
25. Kohli, R.K., Singh, H.P., and Batish, D.R. 2001. Allelopathy in agroecosystems. Food Products Press, USA, 447 p.
26. Maguire, J.D. 1962. Speed of germination- aid selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177. [DOI:10.2135/cropsci1962.0011183X000200020033x]
27. Malick, C.P. and Singh, M.B. 1980. In plant enzymology and histo enzymologhy. Kalyani Publishers, New Dehli.
28. Mighani, F. 2003. Allelopathy. Partov-e- Vagheeh Publications, 256p. [In Persian].
29. Mishra, A. 2015. Allelopathic properties of Lantana camara. Lnt. International Research Journal of Basic and Clinical Studies, 3: 13-28.
30. Mohamed, F.I. and El-Ashery, Z.M. 2012. Cytogenetic effect of allelochemicals of Brassica nigra extract on Pisum sativum. World Applied Sciences Journal, 20(3): 344-353.
31. Mohammadzadeh, M., Peighambari, S.A., Nabipoor, A.R. and Norouzi, M. 2009. Evaluation of rice genotypes response to salinity stress at seedling stage in hydroponic culture. Journal of Crop Breeding, 1(2): 85-95. [In Persian with English Summary].
32. Monica, F., Josekna, A., Sillero, C. and Rubiales, D. 2007. Intercropping with cereals reduces infection by Orobanche crenata in legumes. Crop Protection, 26(8): 166-1672. [DOI:10.1016/j.cropro.2006.10.012]
33. Nwakanma, N.M.C., Odeigah, P.G.C. and Oboh, B.O. 2009. Genotoxic effects of Gongronema latifolium and Vernonia amygdalina using the Allium test. In: Book of Proceedings, 4th UNILAG Conference and Fair, Nigeria, 21-22 October, 2009, pp: 81-90.
34. Oksman-Caldentey, K.M. and Inzé, D. 2004. Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites. Trends in Plant Science, 9(9): 433-440. [DOI:10.1016/j.tplants.2004.07.006] [PMID]
35. Omidbeigi, R. 2009. Production and processing of medicinal plants. Astan Ghods Razavi publisher. 47-182p. [In Persian].
36. Pandey, S. and Velasco, L. 2005. Trends in crop establishment methods in Asia and research issues. In: Rice is Life Scientific Perspectives for the 21st Century. Toriyama, K., Heong, K.L., Hardy, B. (eds.). International Rice Research Instituteand Tsukuba, Japan International Research Center for Agricultural Sciences, Los Ba~nos, Philippines, pp: 178-181.
37. Regosa, M., and Pedrol, N. 2002. Allelopathy from molecules to ecosystems. Science publishers Gnc. NH. USA. P 12- 195.
38. Rho, B.J. and Kil, B.S. 1986. Influence of phytotoxin Pinus rigida on the selected plants. Journal of Natural Science, Wonkwang University, 5: 19-27.
39. Rodenburg, J. and Demont, M. 2009. Potential of herbicide-resistant rice technologies for sub-saharan Africa. Journal of Agrobiotechnology Management and Economics, 12(3-4): 313-325.
40. Sathis Kumar, D., Banji, D., Harani, A., Tirupathi Rao, A., Nageswar Rao, S., Pavan Kumar, CH. and Santhi Krupa, D. 2013. Screening of polyphenolic compounds in Echinochloa crusgalli Roxb extracts by various analytical techniques. Asian Journal of Chemistry, 25(17): 9848- 9852. [DOI:10.14233/ajchem.2013.15505]
41. Setia, N., Batish, D.R., Singh, H.P. and Kohli, R.K. 2007. Phytotoxicity of volatile oil from Eucalyptus citriodora against some weedy species. Journal of Environmental Biology, 28: 63-66.
42. Sheidai, M., Habibi, M., Azizain, D. and Khatamsaz, M. 2009. Cytology and palynology of the Clematis L. species (Ranunculaceae) in Iran. Acta Botanica Croatica, 68(1): 67-77.
43. Singh, R.J. 2003. Plant cytogenetic. CRC Press, image dueing cell cycle in Allium cepa and Vicia boca Raton, pp: 463.
44. Soltani, A. and Torabi, B. 2014. Design and analysis of agricultural experiments (with SAS programs). Jehad Daneshgahi Mashhad Press, Mashhad, Iran. 431p. [In Persian].
45. Teerarak, M., Charoenying, P. and Laosinwattana C. 2012. Physiological and cellular mechanisms of natural herbicide resource from Aglaia odorata Lour. on bioassay plants. Acta Physiologiae Plantarum, 34(4): 1277-1285. [DOI:10.1007/s11738-011-0923-5]
46. Teerarak, M., Laosinwattana, C. and Charoeying, P. 2010. Evaluation of allelopathic, decomposition and cytogenetic activities of Jasminum officinale L. f. var. grandiflorum (L.) Kob. on bioassay plants. Bioresource Technology, 101: 5677-5684. [DOI:10.1016/j.biortech.2010.02.038] [PMID]
47. Yang, C.M., Lee, C.N. and Chou, C.H. 2002. Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedlings: I. Inhibition of supply orientation. Botanical Bulletin of Academia Sinica, 43: 299-304.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
