Volume 7, Issue 1 ((Spring and Summer) 2020)                   Iranian J. Seed Res. 2020, 7(1): 165-180 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karami L, Hedayat M, Farahbakhsh S. (2020). Effect of salicylic acid priming on seed germination and morphophysiological and biochemical characteristics of tomato seedling (Lycopersicom esculentun). Iranian J. Seed Res.. 7(1), : 11 doi:10.29252/yujs.7.1.165
URL: http://yujs.yu.ac.ir/jisr/article-1-352-en.html
Persian Gulf University, Bushehr , leila.karami@pgu.ac.ir
Abstract:   (6202 Views)


Extended abstract
Introduction: Seed germination is a complex and dynamic stage of plant growth, and seed priming is a technique by which the seeds obtain germination potential physiologically and biochemically before being placed on growth media and facing the ecological conditions of the environment. Seed priming increases yield and antioxidant enzymes in plants by increasing germination and seed vigor and as a result, increases percentage of germination. Several studies have investigated the effect of seed priming with organic materials including salicylic acid on improving seed germination in various plant species. Research results have shown that salicylic acid can be used as a growth regulator to increase the germination of plants. Tomato, with scientific name of Lycopersicon esculentum (Mill), belongs to the Solanaceae family and is widely adapted to different climatic and soil conditions. The aim of this study was to evaluate the effect of different concentrations of Salicylic acid on seed germination and some factors of morphophysiologic and biochemical traits of tomato seedlings.
Materials and methods: This research was conducted as factorial in a completely randomized design, including priming treatment in 3 time frames (12, 18 and 24 hours) with three replications. Priming treatments consisted of salicylic acid (2, 2.5 and 3 mg/l) and distilled water. The measured traits were germination parameters including percentage, time, rate, and uniformity of germination and morphological traits including transplant height, crown diameter, root length, leaf number, and leaf area, shoot and root fresh and dry weight and biochemical traits including chlorophyll, peroxidase enzyme, proline, total nitrogen, potassium, calcium, phosphorus, and sodium.
 Results: The favorable effect of salicylic acid was obtained at the concentration of 3 mg/l on mean germination time compared to the distilled water. The positive effect of salicylic acid was observed on transplant height and leaf area (at the concentration of 3 mg/l at 18 and 24 hours’ time frame), shoot and root fresh and dry weight (at 24 hours) compared to the control. Immersion in distilled water for a period of 12 and 24 hours resulted in the highest root length, while salicylic acid treatment reduced root length significantly. The highest transplant height (14.3 cm), leaf number (34), chlorophyll index (59), peroxidase enzyme (10873 unit/g.min-1), total nitrogen (2.89%), potassium (9.81%), and proline content (14.80 µM/g fresh weight) were observed in 24 hours treatment with concentration of 3 mg / l salicylic acid.
Conclusion: According to the results of this study, salicylic acid at certain concentration improves seeds germination of tomato plants through the regulation of physiologic and biochemical processes. It seems that salicylic acid led to increase in plant growth and improvement of seed germination and morphophysiological parameters of the tomato via affecting cell growth and division. Seed priming with salicylic acid at the concentration of 3 mg/l and in longer time frames had positive effect on most traits, whereas the results for each trait were different in relation to priming time.
 
Highlights:
1-Priming of tomato seed in distilled water for 18 hours reduces the time of seed germination.
2-Salicylic acid can be used as an appropriate pretreatment for producing seedlings with better quantitative and qualitative characteristics by affecting the morpho-physiologic and biochemical properties of tomato seedlings.
Article number: 11
Full-Text [PDF 400 kb]   (1642 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2019/09/28 | Revised: 2021/05/23 | Accepted: 2020/04/18 | ePublished: 2020/11/29

References
1. Abdolahi, M. and Shekari, F. 2013. Effect of priming by salicylic acid on the vigor and performance of wheat seedlings at different planting dates. Cereal Research, 3(1): 17-32. [In Persian with English Summary].
2. Ahmadpour Dehkordi, S. and Baluchi, H.R. 2013. Effect of seed priming on antioxidant enzymes and lipids peroxidation of cell membrane in Black Cumin (Nigella sativa L.) Seedling under salinity and drought stress. Electronic Journal of Plant Production, 5(4): 63-85. [In Persian with English Summary].
3. Alamri, S.A., Siddiqui, M.H., Al-Khaishani, M.Y. and Hayssam, M.A. 2018. Response of salicylic acid on seed germination and physio-biochemical changes of wheat under salt stress. Acta Scientific Agriculture, 2(5): 36-42.
4. Agah, F. and Nabavi Kalat, S.M. 2013. Study on the effects of priming on improving the germination indices of lentil (Lens culinaris Melik.) seeds under salt stress. Journal of Seed Science and Technology, 3(2): 53-61. [In Persian with English Summery].
5. Apel, K. and Hirt, H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55: 373-399. [DOI:10.1146/annurev.arplant.55.031903.141701] [PMID]
6. Awasthi, P., Karki, H., Bargali, K. and Bargali, S.S. 2016. Germination and seedling growth of pulse crop (Vigna spp.) as affected by soil salt stress. Current Agriculture Research Journal, 4(2): 159-170. [DOI:10.12944/CARJ.4.2.05]
7. Azarnia, M., Biabani, A., Eisvand, H.R., Gholamalipour Alamdari, E. and Safikhani, S. 2016. Effect of seed priming with gibberellic acid and salicylic acid on germination characteristic and seed and seedlings physiological quality of lentil (Lens culinaris). Iranian Journal of Seed Research, 3(1): 59-73. [In Persian with English Summery].
8. Bates, L.S., Waldren, R.P. and Tevre, I.V. 1973. Rapid determination of free proline for water- stress studies. Plant and Soil, 39: 205- 207. [DOI:10.1007/BF00018060]
9. Black, C.A. 1982. Method of soil analysis. Vol.2, Chemical and microbiological properties. American Society of Agronomy, Madison, USA. 995p.
10. Chance, A. and Maehly, A. C. 1955. Assay of catalases and proxidase. Meth. Enzymol, 2: 764-775. [DOI:10.1016/S0076-6879(55)02300-8]
11. Cavusoglu, K. and Kabar, K. 2010. Effects of hydrogen peroxide on the germination and early seedling growth of barley under NaCl and high temperature stresses. EurAsian Journal of BioScience, 4: 70-79. [DOI:10.5053/ejobios.2010.4.0.9]
12. Daneshmand, F., Arvin, M. J., Keramat, B. and Momeni, N. 2012. Interactive effects of salt stress and salicylic acid on germination and plant growth parameters of maize (Zea mays L.) under field conditions. Journal of Plant Process and Function, 1: 57-70
13. Delian, E.L.E.N.A., Bădulescu, L., Dobrescu, A., chira, L. and Lagunovschi-Luchian, V. 2017. A brief overview of seed priming benefits in tomato. Romanian Biotechnological Letters, 22(3): 12505-12513.
14. Derderian, M.D. 1961. Determination of calcium and magnesium in plant material with EDTA. Analytical Chemistry, 33(12): 1796-1798. [DOI:10.1021/ac60180a051]
15. Gautam, S. and Singh, P.K. 2009. Salicylic acid induced salinity tolerance in corn grown under NaCl stress. Acta Physiologiae Plantarum, 31: 1185-1190. [DOI:10.1007/s11738-009-0338-8]
16. Gill, S.S. and Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48(12): 909-930. [DOI:10.1016/j.plaphy.2010.08.016] [PMID]
17. Ghassemi-Golezani, K., Chadordooz-jeddi, A., Nasrollahzadeh, S. and Moghaddam, M. 2010. Effects of hydro-priming duration on seedling vigour and grain yield of pinto bean (Phaseolus vulgaris L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1): 109-113.
18. Gholamalipour, R. 2010. Effect of seed priming on vegetative growth and salinity tolerance in eggplant seedlings under salinity stress conditions (Cucurbita pepo var. Styriaca). Journal of Agronomy and Plant Breeding, 6(2): 43-52. [In Persian with English Summary].
19. Gunes, A., Inal, A., Alpaslan, M., Eraslan, M., Bagci, F. and Cicek, N. 2007. Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maze (Zea mayz L.) grown under salinity. Journal of Plant Physiology, 164: 728-736. [DOI:10.1016/j.jplph.2005.12.009] [PMID]
20. Hall, J.C., Vaneerd, L.L., Miller, S.D., Owen, M.D.K., Prather, T.S., Shaner, D.L., Singh, M., Vaughn, K.C. and Weller, S.C. 2000. Future research direction for weed science. Weed Technology, 14: 647-658. [DOI:10.1614/0890-037X(2000)014[0647:FRDFWS]2.0.CO;2]
21. Harris, D., Pathan, A.K., Gothkar, P., Joshi, A., Chivasa, W. and Nyamudeza, P. 2001. On-farm seed priming: using participatory methods to revive and refine a key technology Agricultural Systems, 69: 151-164. [DOI:10.1016/S0308-521X(01)00023-3]
22. Havre, G.N. 1961. The flame photometric determination of sodium, potassium and calcium in plant extracts with special reference to interference effects. Analytica Chimica Acta, 25(6): 557-566. https://doi.org/10.1016/S0003-2670(01)81614-7 [DOI:10.1016/0003-2670(61)80134-7]
23. Hayat, S., Fariduddin, Q., Ali, B. and Ahmad, A. 2005. Effect of salicylic acid on growth and enzyme activities of wheat seedlings. Acta Agronomica Hungarica, 53(4): 433-437. [DOI:10.1556/AAgr.53.2005.4.9]
24. Hochmuth, G.J. and Hochmuth, R.C. 2013. Production of greenhouse Tomatoes-Florida greenhouse vegetable production handbook. University of Florida. Institute of Food and Agricultural Sciences. Corporate contributor: Florida Cooperative Extension.
25. Hus, J.L. and Sung, J.M. 1997. Antioxidant role of glutation associated with accelerated agina and hydration of triploid watermelon seeds. Physiologa Plantarum, 100: 967-974. [DOI:10.1034/j.1399-3054.1997.1000424.x]
26. Isvand, H., Azarnia, M., Nazarian Firoozabadi, F. and Sharafi, R. 2012. Effects of priming by gibberellin and abcsisic acid on emergence and some physiological characters of Chikpea (Cicer arietinum L.) seedling under dry and irrigated conditions. Iranian Journal of Field Crop Science, 42(4): 789-797. [In Persian with English Summary].
27. Jini, D. and Joseph, B. 2017. Physiological mechanism of salicylic acid for alleviation of salt stress in rice. Rice Science, 24(2): 97-108. [DOI:10.1016/j.rsci.2016.07.007]
28. Kabiri, R., Hatami, A. and Naghizadeh, M. 2014. Effect of drought stress and its interaction with salicylic acid on Fennel (Foeniculum vulgare Mill.) germination and early seedling growth. Journal of Medicinal Plants and By-Product, 3(2): 107-116.
29. Khan, N., Syeed, S., Masood, A., Nazar, R., and Iqbal, N. 2010. Application of salicylic acid increases contents of nutrients and antioxidative metabolism in mungbean and alleviates adverse effects of salinity stress. International Journal of Plant Biology, 1(1): e1-e1.‏ [DOI:10.4081/pb.2010.e1]
30. Khodary, S.E.A. 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. International Journal of Agriculture and Biology, 6(1): 5-8.
31. Masoumi Zavarian, A., Yousefi Rad, M. and Asghari, M. 2015. The effect of salicylic acid pre-treatment on the characteristics of sting and biochemical characteristics of Marigold (Silybum marianum L.) in salinity. Journal of Seed Research, 5(2): 40-48. [In Persian with English Summary].
32. McDonald, M.B. 2000. Seed priming. (Black M. and Bewley, J.D. Eds.). Sheffield Academic Press. 287-325.
33. Misra, N., and Saxena, P. 2009. Effect of salicylic acid on proline metabolism in lentil grown under salinity stress. Plant Science, 177(3): 181-189. [DOI:10.1016/j.plantsci.2009.05.007]
34. Mohammadi, L. and Shekari, F. 2015. Examination the effects of hydro-priming and priming by salicylic acid on lentil aged seeds. International Journal of Agriculture and Crop Sciences, 8(3): 420-426.
35. Mohammadi, L., Shekari, F., Saba, J. and Zangani, E. 2017. Effects of priming with salicylic acid on safflower seedlings photosynthesis and related physiological parameters. Journal of Plant Physiology & Breeding, 7(1): 1-13. [In Persian with English Summary].
36. Mohammadi, L., Shekari, F., Saba, J. and Zangani, E. 2011. Seed priming by salicylic acid affected vigor and morphological traits of safflower seedlings. Modern Agricultural Science, 7(2): 63-72. [In Persian with English Summary].
37. Ogbaji, P.O., Shahrajabian, M.H. and Xue, X. 2013. Changes in germination and primarily growth of three cultivars of tomato under diatomite and soil materials in auto-irrigation system. International Journal of Biology, 5(3): 80. [DOI:10.5539/ijb.v5n3p80]
38. Panda, S.K. and Patra, H.K. 2007. Effect of salicylic acid potentiates cadmium-induced oxidative damage in Oryza sativa L. leaves. Acta Physiologiae Plantarum, 29(6): 567-575. [DOI:10.1007/s11738-007-0069-7]
39. Sakhabutdinova, A.R., Fatkhutdinova, D.R., Bezrukova, M.V. and Shakirova, F.M. 2003. Salicylic acid prevents the damaging action of stress factors on wheat plants. Bulgarian Journal of Plant Physiology, 21: 314-319.
40. Shakirova, F.M. 2007. Role of hormonal system in the manifestation of growth promoting and anti-stress action of salicylic acid. Pp. 69-89. In: Hayat, S. and Ahmad, A. (eds). Salicylic Acid. A Plant Hormone. Springer, Netherlands. [DOI:10.1007/1-4020-5184-0_4]
41. Shekari, F., Baljani, R., Saba, J., Afsahi, K. and Shekari, F. 2010. Effects of priming by salicylic acid on growth traits of borago (Borago officinalis). Modern Agriculture Science, 18: 47-53. [In Persian with English Summary].
42. Wang, Y.S., Wang, J., Yang, Z.M., Wang, Q.Y., Lu, B., Li, S.Q. and Sun, X. 2004. Salicylic acid modulates aluminum-induced oxidative stress in roots of Cassia tora. Acta Botanica Sinica-English Edition, 46(7): 819-828.
43. Yanik, F., Aytürk, O., Çetinbaş-Genç, A. and Vardar, F. 2018. Salicylic acid-induced germination, biochemical and developmental alterations in rye (Secale cereale L.). Acta Botanica Croatica, 77(1): 45-50. [DOI:10.2478/botcro-2018-0003]
44. Zarandi, M., and Khajeh Hosseini, M. 2016. Influence of weed interaction and priming of seeds on growth indices of different masses of watermelon (Citrus lanatus) seed. Journal of Plant Production Research, 26(1): 31-51. [In Persian with English Summary].
45. Zhang, M., Wang, Z., Yuan, L., Yin, C., Cheng, J., Wang, L. and Zhang, H. 2012. Osmopriming improves tomato seed vigor under aging and salinity stress. African Journal of Biotechnology, 11(23): 6305-6311. [DOI:10.5897/AJB11.3740]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb


This work is licensed under a Creative Commons Attribution 4.0 International License.