Volume 6, Issue 1 ((Spring and Summer) 2019)
Iranian J. Seed Res. 2019, 6(1): 173-184 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sayedena V, Pilehvar B, Abrari-Vajari K, Zarafshar M, Eisvand H R. (2019). Effects of TiO2 Nanoparticles on Germination and Primary Growth of Mountain Ash (Sorbus luristanica). Iranian J. Seed Res.. 6(1), : 12 doi:10.29252/yujs.6.1.173
URL: http://yujs.yu.ac.ir/jisr/article-1-336-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-336-en.html
Effects of TiO2 Nanoparticles on Germination and Primary Growth of Mountain Ash (Sorbus luristanica)
Lorestan University , pilehvar.b@lu.ac.ir
Abstract: (10227 Views)
Extended Abstract
Introduction: Production of nanoparticles and their use are on the rise in different areas of plant science. However, in spite of their increasing production, there is limited information about their effects on plant biology. In the current study, the potential of TiO2 nanoparticles was investigated for the purpose of improving seed germination of Sorbus luristanica and then subsequent effects of nanoparticles on the growth and biomass of the plants were determined.
Materials and Methods: Seeds of S. luristanica were collected from its natural stands. The seeds were primed with different concentrations of 0, 75, 150, 250, 350 and 500 TiO2 nanoparticles miligeram per liter for 24 h. The treated seeds were placed in wet sand at room temperature for 2 weeks and then in cold for 3 months. The expriment was set as a completely randimized design with 4 replications. Aftre 3 months of stratification in moistened sand, the stratified seeds were put in the germinator and with the appearance of seed germination signs, germination data were recorded daily during 22 days. At the end of the seed germination experiment, some germination parameters such as seed germination percentage, seed vigority and mean time to germination were calculated. Moreover, some growth and biomass parameters including leaf number, plant height and dry and fresh biomass of leaf, stem as well as roots were measured. In addition, scaning electron microscopic (SEM) was used for observation of presence and adhesiveness of TiO2 nanoparticles on the seed coat.
Results: Based on the results, all the germination parametres including seed germination percentage, seed vigoroty and mean germination time were improved by the TiO2 nanoparticles treatments. In addition, 500 mg.L-1 treatment considerably improved seed germination characteristics. The peresence of TiO2 nanoparticles on the treated seeds and lack of the nanomatreials on the conrtol seeds were obsereved by scaning electron microscopic pictures. The One-way ANOVA showed that 75 mg.L-1 treatment was more succesful for improving the grwoth (such as shoot length) and biomass production (fresh and dry biomass of leaf, stem and root and total biomass as well).
Conclusion: It can be concluded that priming of the seeds of this species with different concentrations of TiO2 nanoparticles leads to improvement of seed germination and growth and biomass parameters. However, the patterns of effects were different in each phase. Therefore, the objectives should be formulated first and then the best concentration should be chosen. It seems that with appropriate concentrations, nanoparticles can be useful for breaking seed dormancy and production of the species. Given the promising resutls of 150 mg.L-1 treatment, it can represent a successful treatment for breaking seed dormancy and seedling production of S. luristanica.
Highlights:
1- Study of seed germination of Sorbus luristanica for the first time
2- Using Nano-materials and their potentials in breaking seed dormancy and improving the species germination
3- Using SEM in order to study presence and adhesiveness of nanoparticles on the seed coat
Article number: 12
Type of Study: Research |
Subject:
General
Received: 2018/05/23 | Revised: 2021/03/13 | Accepted: 2018/12/4 | ePublished: 2019/09/29
Received: 2018/05/23 | Revised: 2021/03/13 | Accepted: 2018/12/4 | ePublished: 2019/09/29
References
1. Ahmadloo, F., Tabari, M., Rahmani, A., and Yousefzadeh, H, 2011. Effect of cattle manure and decomposed litter to improve germination and survival of Cupressus arizonica and C. sempervirens var. horizontalis in nursery. Journal of Forest and Wood Products (JFWP) (Iranian Journal of Natural Resources), 63(4): 317-330. [In Persian with English Summary].
2. Esmaeili Sharif, M., Hosseini Nasr, S.M., Ghamari Zare, A., and Talebi, M. 2016. Appropriate methods for breaking seed dormancy of Iranian mountain ash (Sorbus persica Hedl.). Iranian Journal of Forest and Poplar Research, 23(4): 694-706. [In Persian with English Summary].
3. Fan, R., Huang, Y.C., Grusak, M.A., Huang, C.P., and Sherrier, D.J. 2014. Effects of nano-TiO2 on the agronomically-relevant Rhizobium-legume symbiosis. Science of the Total Environment, 466-467: 503-512. [DOI:10.1016/j.scitotenv.2013.07.032] [PMID]
4. Fathi, Z., Khavari Nejad, R.A., Mahmoodzadeh, H., and Nejad Satari, T. 2017. Investigating of a wide range of concentrations of multi-walled carbon nanotubes on germination and growth of castor seeds (Ricinus communis L.). Journal of Plant Production Research, 57(3): 228-236. [DOI:10.1515/jppr-2017-0032]
5. Feizi, H., Kamali, M., Jafari, L., and Rezvani Moghaddam, P. 2013. Phytotoxicity and stimulatory impacts of nanosized and bulk titanium dioxide on fennel (Foeniculum vulgare Mill). Chemosphere, 91: 506-511. [DOI:10.1016/j.chemosphere.2012.12.012] [PMID]
6. Feizi, H., Rezvani Moghaddam, P., Shahtahmassebi, N., and Fotovat, A. 2012. Impact of bulk and nanosized titanium dioxide (TiO2) on wheat seed germination and seedling growth. Biological Trace Element Research, 146(1): 101-106. [DOI:10.1007/s12011-011-9222-7] [PMID]
7. Gao, F., Liu, C., Qu, C., Zheng, L., Yang, F., Su, M., and Hong, F. 2008. Was improvement of spinach growth by nano-TiO2 treatment related to the changes of Rubisco activase? Biometals, 21(2): 211-217. [DOI:10.1007/s10534-007-9110-y] [PMID]
8. Gao, J., Xu, G., Qian, H., Liu, P., Zhao, P., and Hu, Y. 2013. Effects of nano-TiO2 on photosynthetic characteristics of Ulmus elongata seedlings. Environmental Pollution, 176: 63-70. [DOI:10.1016/j.envpol.2013.01.027] [PMID]
9. Haghighi, M., Afifipour, Z., and Mozafarian, M. 2012. The effect of N-Si on tomato seed germination under salinity levels. Journal of Biological and Environmental Sciences, 6(16): 87-90.
10. Haghighi, M., and Pessarakli, M. 2013, Influence of silicon and nano-silicon on salinity tolerance of cherry tomatoes (Solanum lycopersicum L.) at early growth stage. Scientia Horticulturae, 161: 111-117. [DOI:10.1016/j.scienta.2013.06.034]
11. Hatami, M., Ghorbanpour, M., and Salehiarjomand, H. 2014. Nano-anatase TiO2 modulates the germination behavior and seedling vigority of some commercially important medicinal andaromaticplants. Journal of Biological and Environmental Sciences, 8(22): 53-59.
12. Huang, Z., Zhang, X., Zheng, G., and Gutterman, Y. 2003. Influence of light, temperature, salinity and storage on seed germination of Haloxylon ammodendron. Journal of Arid Environments, 55(3): 453-464. [DOI:10.1016/S0140-1963(02)00294-X]
13. Jaberzadeh, A., Moaveni, P., Tohidi Moghadam, H.R., and Modari, A. 2010. Effects of TiO2 NPs foliar spraying on the weat under drought stress. Iranian Journal of plant Eco- Physiology, 4(2): 295-301. [In Persian with English Summary].
14. Kamali, N., and Sadeghipoor, A. 2015. Effects of different concentrations of nano TiO2 on germination and early growth of five range plant species. Journal of Rangeland, 9(2): 170-181. [In Persian with English Summary].
15. Khot, L.R., Sankaran, S., Maja, J.M., Ehsani, R., and Schuster, E.W. 2012. Applications of nanomaterials in agricultural production and crop protection a review. Crop Protection, 35: 64-70. [DOI:10.1016/j.cropro.2012.01.007]
16. Kulkarni, M.G., Street, R.A., and Staden, J.V. 2007. Germination and seedling growth requirements for propagation of Diosscorea dregeana (Kunth) Dur.and Schinz-A tuberous medicinal plant. South African Journal of Botany, 73(1): 131-137. [DOI:10.1016/j.sajb.2006.09.002]
17. Kurepa, J., Paunesku, T., Vogt, S., Arora, H., Rabatic, B.M., Lu, J., Wanzer, M.B., Woloschak, G.E., and Smalle, J.A. 2010. Uptake and distribution of ultra-small anatase TiO2 Alizarin red S nanoconjugates in Arabidopsis thaliana. Nano letters, 10(7): 2296-2302. [DOI:10.1021/nl903518f] [PMID] [PMCID]
18. Lafond, G.P., and Baker, R.J. 1986. Effects of temperature, moisture stress, and seed size on germination of nine spring wheat cultivars. Crop Science, 26(3): 563-567. [DOI:10.2135/cropsci1986.0011183X002600030028x]
19. Laware, S.L. and Raskar, S.V. 2014. Effect of titanium dioxide nanoparticles on hydrolytic and antioxidant enzymes during seed germination in onion. International Journal of Current Microbiology and Applied Sciences, 3(7): 749-760.
20. Li, B., Xie, Y., Zhang, Q., Zhang, C., Lu, K. and Tao, G. 2011. Effects of nano-TiO2 on photosynthetic characteristics of Indocalamus barbatus. Journal of Northeast Forestry University, 39: 22-25.
21. Mohammadi, R., Maali Amiri, R., and Abbasi, A. 2013. Effect of TiO2 Nanoparticles on Chickpea Response to Cold Stress. Biological Trace Element Research, 152: 403-410. [DOI:10.1007/s12011-013-9631-x] [PMID]
22. Monica, R.C., and Cremonini, R. 2009. Nanoparticles and higher plants. Caryologia, 62(2): 161-165. [DOI:10.1080/00087114.2004.10589681]
23. Moore, M.N. 2006. Do nanoparticles present ecotoxicological risks for the health of the aquatic environment? Environment International, 32(8): 967-976. [DOI:10.1016/j.envint.2006.06.014] [PMID]
24. Naseri, B., and Tabari, M. 2015. Effects of GA3 and stratification on seed germination of field maple (Acer campestre L.). Forest and Wood Products, 68(2): 419-428. [In Persian with English Summary].
25. Owolade, O.F., Ogunleti, D.O., and Adenekan, M.O. 2008. Titanium dioxide affects diseases, development and yield of edible cowpea. Electronic Journal of Environmental, Agricultural and Food Chemistry, 7(5): 2942-2947.
26. Pais, I. 1983. The biological importance of titanium. Journal of Plant Nutrition, 6(1): 3-131. [DOI:10.1080/01904168309363075]
27. Panwar, P., and Bhardwaj, S.D. 2005. Handbook of practical forestry. Agrobios (India), 191p.
28. Pazhouhan, I., Jalali, S.Gh.A., Atabati, H., Zarafshar, M., and Sattarian, A. 2016. Comparison of carbon nanotubes with chemical and physical treatments to break seed dormancy of Myrtus communis.L. Journal of Botany Research, 29(2): 300-308. [In Persian with English Summary].
29. Qi, M., Liu, Y. and Li, T. 2013. Nano-TiO2 improve the photosynthesis of tomato leaves under mild heat stress. Biological Trace Element Research, 156(1-3): 323-328. [DOI:10.1007/s12011-013-9833-2] [PMID]
30. Seeger, E.M., Baun, A., Kästner, M., and Trapp, S. 2009. Insignificant acute toxicity of TiO2 nanoparticles to willow trees. Journal of Soils and Sediments, 9(1): 46-53. [DOI:10.1007/s11368-008-0034-0]
31. Sidari, M., Mallamaci, C., and Muscolo, A. 2008. Drought, salinity and heat differently affect seed germination of Pinus pinea. Journal of Forest Research, 13(5): 326-330. [DOI:10.1007/s10310-008-0086-4]
32. Sunada, K., Watanabe, T., and Hashimoto, K. 2003. Bactericidal activity of copper-deposited TiO2 thin film under weak UV light illumination. Environmental Science and Technology, 37(20): 4785-4789. [DOI:10.1021/es034106g] [PMID]
33. Wojcik, P., and Klamkowski, K. 2004. "Szampion" apple tree response to foliar titanium application. Journal of Plant Nutrition, 27(11): 2033-2046. [DOI:10.1081/PLN-200030108]
34. Yang, F., Hong, F., You, W., Liu, C., Gao, F., Wu, C., and Yang, P. 2006. Influences of nano-anatase TiO2 on the nitrogen metabolism of growing spinach. Biological Trace Element Research, 110(2): 179-190. [DOI:10.1385/BTER:110:2:179]
35. Yang, Y., Liu, Q., Han, C., Qiao,Y.Z., Yao, X.Q., and Yin, H.J. 2007. Influence of water stress and low irradiance on morphological and physiological characteristics of Picea asperata seedlings. Photosynthetica, 45(4): 613-619. [DOI:10.1007/s11099-007-0106-1]
36. Zhang, P., Cui, H.X., Zhang, Z.J., and Zhong, R.G. 2008. Effects of nano-TiO2 photo- semiconductor on photosynthesis of cucumber plants. Chinese Agricultural Science Bulletin, 24: 230-233.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
