Volume 3, Issue 1 ((Spring and Summer) 2016)                   Iranian J. Seed Res. 2016, 3(1): 109-121 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Tabatabaei S A, Ansari O. (2016). Effect of Cu(SO4) Stress and Plant Growth Regulators on Germination Characteristics and Biochemical Changes of Brassica napus. Iranian J. Seed Res.. 3(1), 109-121. doi:10.29252/yujs.3.1.109
URL: http://yujs.yu.ac.ir/jisr/article-1-139-en.html
Effect of Cu(SO4) Stress and Plant Growth Regulators on Germination Characteristics and Biochemical Changes of Brassica napus
Sayyed Ali Tabatabaei , Omid Ansari
University of Gorgan, Gorgan, Iran , Ansari_o@ut.ac.ir
Abstract:   (38520 Views)

The objective of this research was to evaluate the effect of salicylic acid and gibberellic acid on germination characteristics and changes of proline, protein and catalase activity of Brassica napus seedlings under Cu(SO4) stress. The experimental design was factorial with complete randomized design as a base design with 3 replications. The first factor was 4 levels of Cu(SO4) stress (0, 10, 20 and 30 mg/l), and the second factor was 4 levels of priming with salicylic acid and gibberellic acid 50 mg/l, hydro prime and control (non-priming). Results showed that with increasing levels of Cu(SO4) stress, germination characteristics (germination percentage, germination rate, normal seedling percentage, seedling length and seed vigor index) reduced and using of salicylic acid, gibberellic acid and hydro prime increased germination characteristics. The highest germination percentage (94%), germination rate (30.75 seed per day), normal seedling percentage (86.17%) seedling length (10.53 cm) and seed vigor index (9.08) were attained from priming by salicylic acid 50 ppm under non-stress conditions. Cu(SO4) stress increased proline (35%) and catalase (37%) activity but reduced protein (65%) and priming increased proline, protein and catalase activity as compared to unprimed under stress and control conditions. In this study, using priming treatment salicylic acid had usually higher germination characteristics and catalase activity, total proteins and proline content in comparison with untreated or control seeds.

Keywords: Catalase, Gibberellic acid, Proline, Protein, Salicylic acid
Full-Text [PDF 472 kb]   (4188 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2015/06/20 | Revised: 2017/12/27 | Accepted: 2015/09/29 | ePublished: 2016/11/9
References
1. Aberg, B. 1981. Plant growth regulators. XLI. Monosubstituted benzoic acid. Swedish Journal of Agricultural Research, 11: 93-105.
2. Afzal, I., Basra. S., Farooq, M., and Nawaz, A. 2006. Alleviation of salinity stress in spring wheat by hormonal priming with ABA, salicylic acid and ascorbic acid. Agricultural and Biological, 1: 23-37.
3. Alvarez, M. 2000. Salicylic acid in the machinery of hypersensitive cell death and disease resistance. Plant Molecular and Biology, 44: 429-442. [DOI:10.1023/A:1026561029533] [PMID]
4. Ansari, O., Azadi, M.S., Sharif-Zadeh, F., and Younesi, E. 2013. Effect of hormone priming on germination characteristics and enzyme activity of mountain rye (Secale montanum) seeds under drought stress conditions. Journal of Stress Physiology and Biochemistry, 9(3): 61-71.
5. Ansari, O., Choghazardi, H.R., Sharif Zadeh, F., and Nazarli, H. 2012. Seed reserve utilization and seedling growth of treated seeds of mountain rye (Secale montanum) as affected by drought stress. Cercetări Agronomice în Moldova, 45(2): 43-48. [DOI:10.2478/v10298-012-0013-x]
6. Ashraf, M., and Rauf, H. 2001. Inducing salt tolerance in maize (Zea mays L.) through seed priming with chloride salts: growth and ion transport at early growth stages. Acta Physiologiae Plantarum, 23(4): 407-414. [DOI:10.1007/s11738-001-0050-9]
7. Basra, S.M.A., Farooq, M., Wahid, A., and Khan, M.B. 2006. Rice seed invigoration by hormonal and vitamin priming. Seed Science and Technology, 34(3): 753-758. [DOI:10.15258/sst.2006.34.3.23]
8. Bates, L.S., Waldern, R.P., and Teave, I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39(1): 205-207. [DOI:10.1007/BF00018060]
9. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Nalytical Biochemistry, 72(1-2): 248-254. [DOI:10.1016/0003-2697(76)90527-3]
10. Godbold, D.L. 1994. Aluminium and heavy metal stress: from the rhizosphere to the whole plant. Effects of Acid Rain on Forest Processes. Wiley-Liss New York Vol, 12, 231 p.
11. Haehnel, W. 1984. Photosynthetic electron transport in higher plants. Annual Review of Plant Physiology, 35(1): 659-693. [DOI:10.1146/annurev.pp.35.060184.003303]
12. International Seed Testing Association. 2010. International rules for seed testing.
13. Iqbal, M., and Ashraf, M. 2007. Seed treatment with auxins modulates growth and ion partitioning in salt-stressed wheat plants. Journal of Integrative Plant Biology, 49: 1003-1015. [DOI:10.1111/j.1672-9072.2007.00488.x]
14. Janda, T., Szalai, G., Tari, I., and Paldi, E. 1999. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta, 208(2): 175-180. [DOI:10.1007/s004250050547]
15. John, R., Ahmad, P., Gadgil, K., and Sharma, S. 2008. Effect of cadmium and lead on growth, biochemical parameters and uptake in Lemna polyrrhiza L. Plant Soil and Environmental, 54(6): 262-270.
16. Li, W., Khan, M.A., Yamaguchi, S., and Kamiya, Y. 2005. Effects of heavy metals on seed germination and early seedling growth of Arabidopsis thaliana. Plant Growth Regulation, 46(1): 45-50. [DOI:10.1007/s10725-005-6324-2]
17. Lin, J., Jiany, W., and Liu, D. 2003. Accumulation of copper by roots, hypocotyls, cotyledons and leaves of sunflower (Helianthus annus L.). Bioresource Technology, 86(2): 151-155. [DOI:10.1016/S0960-8524(02)00152-9]
18. Marschner, H., and Rimmington, G. 1988. Mineral nutrition of higher plants. Plant Cell Environment, 11: 147-148. [DOI:10.1111/1365-3040.ep11604921]
19. Munzuroglu, O., and Geckil, H. 2002. Effects of metals on seed germination, root elongation, and coleoptiles and hypocotyls growth in Triticum aestivum and Cucumis sativus. Archives of Environmental Contamination and Toxicology, 43(2): 203-213. [DOI:10.1007/s00244-002-1116-4] [PMID]
20. Noctor, G., and Foyer, C. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Biology, 49(1): 249-279. [DOI:10.1146/annurev.arplant.49.1.249] [PMID]
21. Patade, V.Y., Maya, K., and Zakwan, A. 2011. Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Research Journal of Seed Science, 4(3): 125-136. [DOI:10.3923/rjss.2011.125.136]
22. Satake, M., Mido, Y., Yasuhisa, H., Taguchi, S., Sethi, M.S., and Iqbal, S.A. 1997. Environmental Toxicology. New Delhi: Discovery.
23. Shah, J. 2003. The salicylic acid loop in plant defense. Current Opinion in Plant Biology, 6(4): 365-371. [DOI:10.1016/S1369-5266(03)00058-X]
24. Sharikova, F., Sakhabutdinova, A., Bezrukova, M., Fatkhutdinova, R., and Fatkhudinova, D. 2003. Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Science, 164(3): 317-322. [DOI:10.1016/S0168-9452(02)00415-6]
25. Verma, D.P.S. 1999. Osmotic stress tolerance in plants: role of proline and sulfur metabolisms. Molecular responses to cold, drought, heat and salt stress in higher plants. R. G. Landes Company, Texas,USA, 1999, pp. 153-168.
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb


This work is licensed under a Creative Commons Attribution 4.0 International License.