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Zeinab Zolfaghari, Eadi Bazgir, Arezoo Naghavi,
Volume 9, Issue 1 (3-2020)
Abstract

Z, Bazgir E, Naghavi A (2020) Report of five species of plant parasitic nematodes associated with hawthorn forest trees in western Iran. Plant Pathology Science 9(1):15-29. DOI: 10.2982/PPS.9.1.15
Introduction: The Hoplolaimidae family is one of the largest and economically most important families of the order/ infraorder Tylenchida / Tylenchomorpha. The members of this family spread all over the world and can be found under different climatic conditions. Some species in this family are serious plant parasites and significantly reduce crop yields. Materials and Methods: In the forests of Khorramabad city in western Iran 70 samples were collected from the soil around the roots of hawthorn trees in the period of 2017-2019. After recording the properties of each sample, they were transferred to a laboratory and cooled at 4 °C. The nematodes were extracted from the soil using the tray method, then fixed and transferred to pure glycerin, and permanent slides were made. The morphological and morphometric properties of the nematodes were examined using a light microscope equipped with a Dino Capture camera. The nematode species were identified using scientific identification keys. Results: Five species of plant parasitic nematodes belonging to two genera of Hoplolaimidae were identified in this study, including Rotylenchus goodeyi, Helicotylenchus canadensis, H. digonicus, H. vulgaris and H. tunisiensis. Conclusion: R. goodeyi is reporting as a new record for the nematode fauna of Iran. Morphometrical and morphological characteristics of these nemadodes are described here.

Samira Pakbaz, Mostafa Darvishnia, Arezoo Naghavi,
Volume 11, Issue 1 ((Autumn & Winter) 2022)
Abstract

Pakbaz S, Darvishnia M, Naghavi A (2022) Phylogenetic status of Grapevine fanleaf virus isolate of Lorestan province of Iran. Plant Pathology Science 11(1):1-12.                     Doi: 10.2982/PPS.11.1.1
 
Introduction: Grapevine fanleaf virus (GFLV) is a major grapevine infecting virus in the world. Materials and Methods: Grapes showing GFLV signs were sampled from the suburbs of the city of Khorramabad in Lorestan Province of Iran in spring 2020 and RT-PCR test was performed to amplify their GFLV-CP gene and the product was sequenced. Results: Specific primers were able to amplify a 1515 bp fragment of the CP gene. Based on the nucleotide sequence of this fragment, GFLV was first identified in this region. The nucleotide sequence similarity of this isolate was detected at 89.14-95.64% with other isolates in the NCBI library. Also, the phylogenetic tree of these isolates, based on the genomic CP region, grouped GFLV isolates into two groups I and II. The Lorestan GFLV isolate was placed in a subgroup in Group I together with GFLV isolates from the northwestern part of Iran and the Takestan isolate, and isolates from other countries were grouped in a separate subgroup of this group. Also in the phylogenetic tree, the Northeast isolates and the Fars and Kohgiloyeh & Boyer-Ahmad province isolates were classified in Group II. Conclusion: The results of this research indicate that the virus is endemic and that its likely origin was in Iran and then spread to other parts of the world. The impact of geographic segregation on the evolution of GFLV can also be deduced.

 

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