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Anahita Rashtian, Afagh Tabandeh Saravi, Somaya Naseh Dehabanda,
Volume 3, Issue 1 (9-2016)

Salinity is one of environmental stresses in the world and especially in arid lands and desert that reduces production of plants and it is important to choose salt-resistant plants in all life stages, especially germination. The aim of this study was to investigate the effect of seed source (provenance (on germination and morphological traits of Amygdalus elaegnifolia saplings undersalt stress. The experiment was carried out as factorial in completely randomized design  withthree replicates. Seed source (FereydonShahr, Kazeron and Semirom) and salinity treatment withfour levels (0, 1.5, 3 and 5 dSm-1) using NaCl were considered as first and  second factors, respectively. The measured variables were germination percentage, germination rate, vigor index, shoot length, radicle length, shoot wet weight, radicle wet weight, shoot dry weight, radicle dry weight, total leaf number, and radicle length/shoot length. The results of analysis of variance and mean comparison showed that salinity made significant differences in most of the investigated traits of Amygdalus elaegnifolia. In the control treatment of salinity, significant differences were found among the studied seed sources but the effect of seed sources was not significant in the other levels of salinity. In general, Fereydunshahr was the best of all studied provenances in non-saline conditions (control treatment).  

Somayeh Homayounfar , Roghaiyeh Zolfaghari , Payam Fayyaz ,
Volume 3, Issue 2 (3-2017)

Background and objectives: Plant species with wide geographic ranges, exhibit high tolerance to cold stress. Trees in temperate regions must cope with freezing temperatures, especially in late autumn and winter. One of the mechanisms to tolerate cold stress is through decreasing photoperiod and temperature during acclimation. In addition, provenance of a species could be one of the main factors contributing to cold resistance. Therefore, in the present study, the effect of cold stress on physiological responses of Pistacia atlantica seedlings from three provenances was investigated in southern Zagros to select the most cold-resistant provenance.
Material and methods: Seeds of three provenances, namely Margon, Noor Abad and Yasuj, from southern Zagros, were collected and planted in pots. After germination of seeds, induction of acclimation was carried out at two stages for four weeks. This was accomplished through the simulation of temperature and photoperiod of Yasuj region from September to November. Following that, the indexes of photosystem efficiency and chlorophyll content were measured. After each acclimation stage, leaves and stems of seedlings were exposed to three levels of temperature, +4°C (control), -20°C for one hour, and -20 for two hours. Then, relative water content (RWC) and electrolyte leakage (EL) of stems and leaves were measured. This study was conducted as a factorial with three factors of provenance (three levels), cold treatments (three levels) and acclimation (two levels), adopting a completely random design.
Results: The results showed that EL increased with decreases in temperature and this increase was the highest in the case of the Noor Abad provenance. In addition, the stem RWC of Yasuj and Margoon provenances decreased in the second cold acclimation stage. Chlorophyll content, photosystem efficiency and electron transport rate were lower in Noor Abad provenance, compared with other two provenances. Moreover, except the electron transport rate, all fluorescence parameters significantly decreased during the cold acclimation.
Conclusion: The results showed that measuring EL and fluorescence parameters could be a useful indicator for identifying cold-tolerant provenances. The seedlings of Noor Abad provenance exhibited the least and Margoon provenance showed the highest resistance to cold stress. This could be due to high altitude and cold climate of Margoon provenance.

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