per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
1
13
article
The Partitioning Trend of Resources and Alpha-Amylase Enzyme Activity with Zinc Priming in Wheat (Triticum aestivum) Seed
Amin Abbasi
a.abbasi25@yahoo.com
1
Fariborz Shekari
Shekari_fb@yahoo.com
2
Seyed Bahman Mousavi
Mosavi@yahoo.com
3
Naser Sabaghnia
n.sab1355@yahoo.com
4
Abdollah Javanmard
5
University of Maragheh
University of Maragheh
University of Maragheh
University of Maragheh
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh
The percentage and rate of germination and proper establishment of seedlings are important factors contributing to higher yield per unit area. As an essential element for plant growth and development, Zinc plays a critical role in many metabolic processes, and also has a positive effect on seed germination. The effects of seed priming with concentrations of 0.5, 1 and 2 percent zinc sulfate on the partitioning of reserved materials in seed, the start of autotrophic seedling growth and the activity of alpha-amylase enzyme were investigated in wheat seeds, using a factorial experiment in the Research Farm of the University of Maragheh, Iran in 2014 on the basis of a completely randomized design with four replications. The results showed that zinc priming had a significant effect on all the parameters under investigation. Mean comparisons showed that an increase in zinc concentration- up to one percent- had a positive effect on the parameters investigated and increasing its concentration more than one percent caused a negative effect on the parameters touched upon above. The results of this study showed that the dry seed weight of the control and zinc sulfate (0.5%) treatment reached a stable level on day 21 and 20, respectively. In addition, in the control and 0.5% zinc sulfate, the total dry weight reached its initial weight (seed weight) on day 15 and 13, respectively. In addition, the growth of autotrophic plant began 10-12 days after soaking. The seeds treated with 5.0 and 1 percent zinc sulfate had more leaf area than the other treatments; this could be due to the earlier entrance of the seedlings produced by 5.0 and 1% zinc sulfate treatments into the autotrophic stage, as compared with other treatments. The results of variance analysis of the finishing seeds' reserved materials, initial kernel, root dry weight and shoot dry weight confirmed that 0.5 and 1% zinc sulfate treatments were better than other concentrations. The treatments of 0.5 and 1% of zinc sulfate reached the autotrophic stage sooner than the control and 2% zinc sulfate treatment. Given the results of this study, 5.0 and 1% zinc sulfate treatments were selected for field cultivation.
http://yujs.yu.ac.ir/jisr/article-1-128-en.pdf
Leaf area
Autotrophic growth
Heterotrophic growth
Efficiency of reserve mobilization
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
15
30
article
An Investigation into Germination Patterns of Common Lambsquarters (Chenopodium album) in Reaction to Temperature, Salinity and Waterlogging Stress
Shahram Nazari
shahram_nazari1986@yahoo.com
1
Reza Deihimfard
deihimfard@gmail.com
2
Javad Faraji
javad_f84@yahoo.com
3
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Department of Agroecology, Environmental Sciences Research Institute, Shahid Beheshti University, G.C., Tehran, Iran
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Gaining insights into the germination and emergence patterns of weeds as well as the factors which have an impact on these patterns is beneficial for weeds management programs. In order to investigate the effect of temperature, waterlogging and salinity on germination and emergence of common lambsquarters, three separate experiments were conducted, adopting a completely randomized design with four replications. The results of the temperature experiment showed that the highest germination percentages, which were 87, 93 and 88%, were obtained in the temperature range of 15, 20 and 25°C, respectively. Maximum values of germination rate, radicle length, plumule length, and seedling dry weight were obtained at 20°C. In addition, optimum seed vigor index of 796.29 was observed at 20°C. The results of the Regression Model showed that germination percentage decreased with an increase in waterlogging duration and halted after 8 and 10 days of consecutive waterlogging. Mean comparisons revealed that radicle and plumule length, seedling dry weight and vigor index decreased significantly by increasing the period of waterlogging. Salinity adversely affected germination percentage, germination rate and seedling dry weight of common lambsquarters. These factors were at their highest amounts in the control and 50 mM NaCl treatments. A downward trend was observed in these factors as NaCl concentrations increased and finally the germination process stopped in concentrations ranging from 300 to 400 mM NaCl. Radicle and plumule length also decreased from 10.51 and 9.23 cm in the control treatment to 3.57 and 2.47 cm at 250 mM NaCl. Overall, the results revealed that the maximum seed vigor index of 851.84 was obtained in the control treatment and seed vigor halted when the salinity level increased to more than 250 mM NaCl. Finally, the results showed that optimum temperature for germination of common lamsquarters was 20 °C and the existence of salinity and waterlogging stress can decrease growth indices of this weed at germination and seedling stages.
http://yujs.yu.ac.ir/jisr/article-1-116-en.pdf
Germination percentage
Germination rate
Seed vigor index
Seedling
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
31
39
article
Evaluation of Nonlinear Regression Models to Describe Seed Germination Response of Mountain Rye (Secale mountanum) to Temperature
Omid Ansari
Ansari_o@ut.ac.ir
1
Farshid Ghaderifar
farshidghaderifar@yahoo.com
2
Farzad Sharif zadeh
moradia@yahoo.com
3
Ali Moradi
sharifz@ut.ac.ir
4
of Seed Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
University of Gorgan
University of Tehran
Yasouj University
The present study sought to evaluate the effect of different temperatures on germination and to determine cardinal temperatures (i.e., base, optimum and maximum) of Secale mountanum at temperatures of 3, 5, 10, 15, 20, 25, 30 and 35oC. Three nonlinear regression models (i.e., segmented, dent-like and beta) were used for quantifying the response of germination rate to temperature. The results showed that in addition to germination percentage, the temperature has a significant impact on germination rate. Given the root mean square of errors (RMSE) of germination time, the coefficient of determination (R2), the simple linear regression coefficients a and b, and the relationship between the observed and the predicted germination rates, the best models for determination of cardinal temperatures of Secale mountanum were dent-like and beta models. Base, optimum and maximum temperatures were estimated to be about 2.70 to 3.17, 21.27 to 30.00 and 35.00 to 35.05°C, respectively for the dent-like model. However, given the high value of SE for temperature base and a negative estimate of the base temperature of the beta model, one can report the dent-like model as the right model. Therefore, by using the dent-like model and the estimated parameters, it is possible to use this model for predicting germination.
http://yujs.yu.ac.ir/jisr/article-1-123-en.pdf
Secale mountanum
Cardinal temperatures
Temperature
Model
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
41
55
article
Quantification of Seedling Emergence of Kochia (Kochia indica) Affected by Temperature, Salinity and Seeding Depth
Gholamhassan Ranjbar
ranjbar71@gmail.com
1
Hossein Ghadiri
ghadir@shirazu.ac.ir
2
, National Salinity Research Center, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran
Dept. of Crop Production and Plant Breeding
A controlled experiment was run to quantify emergence of Kochia indica under different temperature, salinity and seeding depth levels at Yazd National Salinity Research Center in 2013. Treatments were five day/night temperature regimes: 20/10, 25/15, 30/20, 35/25 and 40/30 °C, five salinity levels: 2, 6, 10, 14 and 18 dS m-1, and seeding depth on the surface (0 mm), 5, 10 and 15 mm. Final emergence percentage, emergence rate index and elapsed time (days) to reach 50% of the maximum emergence were measured. The results showed that the highest and lowest final emergence percentages were observed at 25/15°C and 40/30°C day/night, respectively. Final emergence percentages at salinity levels of 6, 10, 14 and 18 dS m-1were, respectively, 9, 22, 36 and 57% lower than 2 dS m-1. Final emergence percentages for 5, 10 and 15 mm seeding depths were, respectively, 30, 44 and 72% lower, as compared with the placement of seeds on the soil surface. Regression analysis showed that final emergence percentage linearly decreased with increase in salinity and seeding depth levels. However, elapsed time (days) to reach 50% of the maximum emergence (T50), increased as salinity and seeding depth increased, so that the highest T50 was obtained for 18 dS m-1 and seeding depth of 15 mm. Increase in salinity and seeding depth was associated with a significant decrease in emergence rate index. In addition, using a logistic regression equation, emergence rate of K. indica was quantified on each day after sowing for each temperature-salinity combination to predict the distribution range of the plant in these situations.
http://yujs.yu.ac.ir/jisr/article-1-147-en.pdf
Linear regression
Emergence rate index
Weed
Seeding depth
Logistic equation
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
57
70
article
The Effect of Auxin and Cytokinin on Some Morpho-Physiological and Germination Characteristics of Stevia (Stevia rebaudiana Bertoni) Seeds
Razieh Sarami
r.sarami@shahed.ac.ir
1
Heshmat Omidi
omidi@shahed.ac.ir
2
Abdol amir Bostani
Bostani@shahed.ac.ir
3
Shahed University
, Agricultural College and Medicinal Plant Research Center, Shahed University Tehran, Tehran, Iran
Shahed University
The present study was conducted to investigate the efficiency of hormonal pretreatment on increasing germination and early growth of seedling in the Seed Technology Laboratory of Shahed University in 2015 as a factorial experiment, adopting a completely randomized design with 4 replications. The treatments were: 5 levels of IAA (indole-3-acetic acid) namely, zero, 0.1, 0.5, 1 and 1.5 mg/L and 5 levels of PBA (Tetrahydro pyranyl benzyl adenine), which were zero, 0.5, 1, 1.5 and 2 mg/L. Analysis of variance showed that the use of the two hormones and their interactions had a significant effect on all traits such as seed germination, biomass, leaf relative water content and photosynthetic pigments. The highest percentage of germination (66.66%) was obtained in 1.5 mg/L IAA with 1 mg/L PBA. 0.5 mg/L PBA and in 0.1 mg/L IAA the longest shoot (1.28 and 1.17 cm, respectively) was obtained. Germination coefficient decreased by about 12.5% by increasing IAA from 0.1 to 1.5 mg/L. The greatest relative water content (42.73% and 37.38%) was obtained with 0.5 mg/L PBA and 1.5 mg/L IAA, respectively. Combination of IAA+PBA (0.1+0 mg/L) had a positive effect on both the length of the root and seedling. The high concentration of PBA and the lowest amount of IAA had similar results in terms of plant biomass. The highest plant biomass (4.33 mg) was obtained in seeds treated with 2 mg/L of cytokinin and 0.1 mg/L of auxin. Photosynthetic pigments were also affected by these two hormones through the IAA was more effective than PBA. The finding was that auxin and cytokinin increase germination and improve the morpho-physiological indicators and thus increase the yield of Stevia.
http://yujs.yu.ac.ir/jisr/article-1-190-en.pdf
Indole-3-acetic acid
Tetra hydro pyranyl benzyl adenine
Germination coefficient
Biomass
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
71
88
article
The Study of Seed Germination and Dormancy of Chenopodium album, Convolvulus arvensis and Setaria viridis in Pistachio Orchards of Rafsanjan, Iran
Mostafa Alinaghizadeh
m.a.naghizadeh@gmail.com
1
Mohammad Khajeh-Hosseini
Saleh@um.ac.ir
2
Seyed Ahmad Hosseini
s.ah_hosseini@yahoo.com
3
Mohammad Hasan Rashed Mohasel
mhrashed@um.ac.ir
4
of Department of Agricultural, Payame Noor University (PNU), Iran
Department of Crop Science, Ferdowsi University of Mashhad, Iran
Department of Agronomy Science, Vali-e-Asr University of Rafsanjan
Department of Crop Science, Ferdowsi University of Mashhad, Iran
In order to study the seed germination behavior and dormancy breaking methods of three weed species (i.e., Chenopodium album, Convolvulus arvensis and Setariaviridis) of pistachio orchards in Rafsanjan, Iran, three separate factorial experiments (with 2 factors) were conducted based on a completely randomized design with four replications, at the Faculty of Agriculture, Vali-E-Asr University of Rafsanjan, Iran, in 2014. Weed seeds were collected from five different regions of Rafsanjan, such as Markazi, Anar, Koshkoiyeh, Kabotarkhan and Nogh. Dormancy breaking treatments for Chenopodium album involved distilled water (control), KNO3 (at 500 and 1000 ppm), chemical scarification by sulfuric acid (for 5 and 10 min), and cold stratification (for 1, 3 and 5 weeks). Treatments for Convolvulus arvensis involved distilled water (control), scarification by sandpaper, chemical scarification by sulfuric acid (20 and 30 min), and boiling water (for 15 and 30 min). Treatments for Setaria viridis involved distilled water (control), gibberellic acid (250, 500 and 1000 ppm), KNO3 (500 and 1000 ppm), and cold stratification (for 1, 3 and 5 weeks). The results showed that seed germination percentage (SGP) and mean germination time (MGT) of three weed species were significantly different among weed populations and dormancy breaking methods. For Chenopodium album, cold stratification of 5 weeks resulted in highest SGP (97%) in Nogh population. For Convolvulus arvensis and Setaria viridis, the highest SGP was obtained after scarification by sandpaper (98% in Kabotarkhan population) and using 1000 ppm gibberellic acid (60% in Kabotarkhan population), respectively. In addition, increasing the weight of 1000 seeds in the three weed species in question increased SGP.
http://yujs.yu.ac.ir/jisr/article-1-218-en.pdf
Cold Stratification
Gibberellic Acid
KNO3
Scarification
Seed Dormancy
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
89
103
article
Effect of Accelerated Ageing and Natural Storage on Germination, Seedling Growth and Reserves Depletion of Chickpea (Cicer arietinum) Seeds
Mehdi Shaban
Shaaban.mehdi@gmail.com
1
Farshid Ghaderifar
farshidghaderifar@yahoo.com
2
Hamidreza Sadeghipour
HRsadeghipour@gmail.com
3
Ahad Yamchi
yamchi@gau.ac.ir
4
in Seed Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
This study was conducted to evaluate the effects of accelerated aging and natural storage on seed germination and seedling heterotrophic growth of chickpea in Gorgan University of Agricultural Sciences and Natural Resources in 2014. The experiment was carried out, adopting a completely randomized design with four replications. Treatments were 8 aging levels (i.e., 2 years, and 4 years natural storage; 1, 2, 3, 4, 5 days of accelerated aging and a control). The results showed that the effect of aging treatment on all the traits was significant. Reduction of germination percentage, germination rate, root and shoot length seed vigor index and seedling dry weight of 4 and 5 accelerated aging days was higher than 2 and 4 natural storage years. The electrical conductivity of seed lots increased by an increase in accelerated aging to 4 and 5 days, which was higher than 2 and 4 natural storage years. This is due to incapability of a membrane to keep its permeability, which is the result of the higher sensitivity of seeds to accelerated aging. Reduction of the rate and efficiency of reserves used and also dynamic reserves in natural storage was lower than 4 and 5 accelerated aging days. However, maximum rate and efficiency of reserves used and also dynamic reserves were obtained at 2 accelerated aging days. This could be due to increase in repair reaction rates under these conditions and activation of hydrolytic enzymes in seeds. Finally, the results of the present study revealed that damages to chickpea seed at 4 and 5 accelerated aging days are more than 2 and 4 natural storage years, which leads to the reduction of germination percentage and rate.
http://yujs.yu.ac.ir/jisr/article-1-165-en.pdf
Storage
Aging
Efficiency of reserves used
Seed quality
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
105
118
article
Effect of Chitosan on Seed Germination and Biochemical Traits of Milk Thistle (Silybum marianum) Seedling under Salt Stress
Mehdi Aghighi Shahverdi
aghighim@yahoo.com
1
Heshmat Omidi
heshmatomidi@yahoo.com
2
Sayed Esmail Mousavi
Esmaeil.m1370@gmail.com
3
of Crop Physiology, Faculty of Agriculture, Shahed University, Tehran, Iran
For the purpose of evaluating the effect of chitosan on seed germination and some biochemical characteristics of the milk thistle herb in the conditions of salinity, an experiment was conducted as factorial in a completely randomized design (CRD) with three replications in the Laboratory of Seed Science and Technology of Shahed University, Tehran in 2015. Experimental factors comprised salinity levels (0, 4, 8 and 12 dS.m-1) and different levels of Chitosan (0, 0.25, 0.5, 0.75 and 1 percent). The results showed that salt stress reduced germination percentage, germination coefficient, germination speed, weight and length vigor index, radical, plumule and seedling length and total biomass and increased mean germination time. Seed priming with chitosan up to 0.5% concentration increased germination coefficient, weighted index vigor and plumule length. The highest amounts of total chlorophyll and total protein were obtained in seed priming with 0.5% chitosan levels in zero salinity level (control). By increasing salinity levels, the activity level of catalase and peroxidase increased, so that the highest level of the activity of these two enzymes was obtained in the salinity level of 12 dS.m-1 in pre-treatment with 0.5% Chitosan. The results showed that seed priming with chitosan of 0.5% could reduce harmful effects of salt stress on some traits of milk thistle seedlings and could even improve their growth.
http://yujs.yu.ac.ir/jisr/article-1-224-en.pdf
Catalase
Chlorophyll
Milk thistle
Peroxidase
Protein
Seed vigor
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
119
127
article
Effect of Titanium Dioxide Nanoparticles and Carbon Nanotube on Seed Germination Characteristics of Some Hordeum vulgare Hulled Varieties
Mahmod reza Tadayon
mrtadayon@yahoo.com
1
Mohammad Rahimi
mohammadrahimi566@gmail.com
2
of Seed Science and Technology of Shahrekord University, Shahrekord, Iran
College of Agriculture University Shahrekord
The purpose of this study was to evaluate the effect of Nano TiO2 and Nano CNT on some germination indices and growth parameters of some hulled barley cultivars. The experiment was conducted in a laboratory at Shahrekord University during 2014. The study was a factorial, adopting a completely randomized design with four replications. Treatments consisted of titanium dioxide nanoparticles and carbon nanotube (CNT) in four concentrations (0, 10, 30 and 60 mg.l-1) which were applied to seeds of hulled barley cultivars such as Bahman, Makoii and Nosrat. The traits measured were the dry weight of seedling and seedling length, germination percentage, germination rate, mean germination time, the percentage of resistance of radical, seedling vigor length and weight index. The results showed that carbon nanotubes treatments with 60 mg.l-1 had a significant impact on germination percentage, germination rate, seedling vigor length and weight index and seedling dry weight, as compared with other treatments. In this experiment, the highest percentages of resistance of radicle, seedling vigor weight index and seedling dry weight of Nosrat Cultivar were obtained under 60 mg.l-1 carbon nanotube treatment, which was 70, 122.2 and 64.9%, respectively, as compared with control treatment. In addition, 60 mg.l-1 carbon nanotube treatment increased the seedling vigor of Makoii cultivar by 39.8%, as compared with the control treatment. The findings were that in terms of seedling vigor length and weight index, seedling length, seedling dry weight and the percentage of resistance of radical, the Nosrat Cultivar showed better morphological characteristics than Bahman and Makoii cultivars, under laboratory conditions.
http://yujs.yu.ac.ir/jisr/article-1-169-en.pdf
Seedling vigor
Nosrat cultivar
Bahman cultivar
Seedling fresh weight
Seedling dry weight
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
129
141
article
Cardinal Temperatures for Germination of Roselle (Hibiscus sabdariffa)
Mahdi Javadzadeh
sm.javadzadeh@stu.um.ac.ir
1
Parviz Rezvani Moghaddam
Rezvani@um.ac.ir
2
Mohammad Banayan-Aval
banayan@um.ac.ir
3
Javad Asili
asilij@mums.ac.ir
4
Ferdowsi University of Mashhad
Department of Agronomy, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Ferdowsi University of Mashhad
Mashhad University of Medical Sciences
Roselle is an important medicinal and industrial plant of the family of Malvaceae, and is planted in vast areas of Sistan and Baluchestan. In a laboratory study, the effect of varying temperatures on seed germination of Hibiscus sabdariffa was investigated and minimum, optimum and maximum temperatures for its germination were determined in a completely randomized design with four replications. For this purpose, temperatures 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50°C were considered in each treatment. Cardinal temperatures for germination were determined consistent with three models (i.e., Intersected-lines Model, Five-Parameters Beta Model and Quadratic Polynomial Model). The traits measured were germination percentage, the speed of germination and mean germination time. The temperature effect on all the measured traits was significant. The results of the regression analysis showed that the best model in terms of cardinal point of this plant is the Five-Parameters Beta Model. Given the results of this model, the minimum and the optimal temperatures for the germination of Roselle are 4.04°C, and 29.83° C, respectively.
http://yujs.yu.ac.ir/jisr/article-1-41-en.pdf
Cardinal temperatures
Germination rate
Plant medicinal
Regression models
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
143
154
article
Effect of Magnesium and Zinc Elements on Agronomic Traits and Seed Germination of Bread Wheat (Triticum aestivum cv. Sivand)
Farhad Sadeghi
fsadeghi40@yahoo.com
1
, of Seed and Plant Improvement Departments, Kermanshah Agricultural and Natural Resources Research Center, AREEO, Kermanshah, Iran
In order to study the effect of zinc and magnesium on agronomic and seed germination traits of wheat (Sivand cultivar), the current study was carried out at Mahidasht Agricultural Research Station in Kermanshah over the years 2012 and 2013. The experiment was conducted as factorial based on completely randomized block design with three replications. The treatments consisted of four levels of zinc sulfate (zero, 20, 40 and 60 kg per hectare) and four levels of magnesium (zero, 70, 140 and 210 kg per hectare in the form of magnesium sulfate). The measured parameters included thousand seed weight, hectoliter weight, protein, zinc and magnesium seeds, germination test, growth rate, seed vigor, plant characteristics and grain yield. The results showed the effects of zinc and magnesium factors were significant for most traits at 1 and 5%. The effect of zinc sulfate showed that 60 kg zinc sulfate had the best effect on the traits under investigation. The effect of this treatment on two important traits (i.e., yield and grain protein percent) with 7.10 tons per hectare and 12.05% was higher (about 115 and 103%, respectively), as compared with the control treatment. Effect of magnesium sulfate levels on the traits showed that the treatment of 210 kg per hectare of magnesium sulfate was the superior treatment. The effects of the above-mentioned treatment on yield (7.84 tons per hectare) and grain protein (11.89 percent) were higher than the control treatment, which was 124 and 101.5%, respectively. Given the number of field nutrients and the wheat needs for these elements, the treatments of 20 kg per ha of zinc sulfate and 140 kg per ha of magnesium sulfate were better than other treatments and economically speaking, are very cost-effective and are thus recommended.
http://yujs.yu.ac.ir/jisr/article-1-105-en.pdf
Wheat
Seed vigor
Grain yield
Zinc
Magnesium sulfate
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
155
169
article
Effect of Breaking Dormancy Treatments on Germination of Ferula assa-foetida Seed
Ahmad Nowruzian
nowruzian@irost.ir
1
Majid Masoumian
masoumian200@yahoo.com
2
Mohammad Ali Ebrahimi
ebrahimi_mpn@yahoo.com
3
Gholam Reza Bakhshi khaniki
bakhshi@pnu.ac.ir
4
Department of Agriculture, Iranian Research Organization for Science and Technology (IROST)
Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
Biotechnology Department of Payame Noor University of Tehran, I.R. Iran
Biotechnology Department of Payame Noor University of Tehran, I.R. Iran
Asafetida (Ferula assa- foetida L.) is an important medicinal plant belonging to Apiaceae family and has long dormancy. In this research, vernalization, washing time, GA3, medium strength, harvesting time and interaction of these treatments were studied to optimize condition of germination. The results showed that vernalization at 4-5°C for two weeks increased germination by 50%, as compared with the control. Maximum and minimum germinations were obtained for 6 and 2 hours’ washing, which was 42% and 20.47%, respectively. Germination of Ferula was increased (41.5%) by using 10 mg/l of GA3, as compared with the control. In addition, using half strength MS media led to a 25% increase in germination. Moreover, germination mean increased by applying these treatments to one-year-old seeds, in comparison with fresh ones (61% and 36%, respectively). By running factorial experiments in the CRD, the best combination of treatments which could significantly increase germination was a combination of vernalization (4-5°C for two weeks), half strength MS media, GA3 (10 mg/l) and washing time (6h). Given the results of the study, for the purpose of breaking the dormancy of Asafetida, it is suggested that use is made of one-year-old seeds, and half strength MS media, along with right combinations of vernalization, washing time and GA3.
http://yujs.yu.ac.ir/jisr/article-1-215-en.pdf
Washing time
Ferula assa-foetida L.
GA3
Germination
Harvesting time
Vernalization
Seed dormancy breaking
per
Yasouj University
Iranian Journal of Seed Research
2383-1251
2383-1480
2017-02
3
2
171
178
article
Application of Chitosan on Soybean (Glycine max) Seed Germination under Salt Stress
vahid mansouri gandomany
mansourivahid2@yahoo.com
1
heshmat omidi
Omidi@shahed.ac.ir
2
Mohammad Rezaei Charmahin
mrze71@gmail.com
3
of Science and Seed Technology, Faculty of Agricultural Sciences, Shahed University, Tehran, Tehran, Iran
In order to investigate the effect of pretreatment of chitosan biological compounds on seeds of soybean (Glycine max L. cv. Williams) under salt stress conditions, a factorial experiment was conducted, adopting a completely randomized design with three replications at Shahed University in 2015. Factors examined included the combination of chitosan at three levels (0, 0.25 and 0.5 the weight-volume) and salinity at 4 levels (0, 5, 5.7 and 10 dS/m). The results showed that the interaction of chitosan and salinity had a significant impact on seed germination characteristics such as germination percentage, germination rate, mean germination time, normal seedling and physiological characteristics such as proline and soluble sugars of shoots. In contrast to salt stress increase of 30%, and an increase of 24 and 35% of proline and soluble sugars of the shoot, pretreatment of chitosan (0.25%) improves seedling germination characteristics of soybeans. Given the impact of the treatments of chitosan on seed germination and seedling growth of soybean, it is possible to introduce 0.25% W/V chitosan as a treatment to deal with conditions of salinity.
http://yujs.yu.ac.ir/jisr/article-1-241-en.pdf
Proline
Priming
Biological compounds chitosan
Soluble sugars