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Showing 3 results for Seed Germination

Mina Arast, Ali Tavili, Saied Shojaei,
Volume 2, Issue 2 (2-2016)
Abstract

A vast area of Iran is covered by sand dunes. Biological control is an appropriate method for sand dune fixation. So, it is essential to recognize characteristics of psammophyte plant species and introducing suitable species for sand dunes. Ammodendron persicum is one of the important and compatible species in desert ecosystems. The current research was carried out to investigate the effect of different treatments on seed dormancy breaking and germination stimulation of Ammodendron persicum in order to determine the most effective treatment in enhancing of germination and primary growth of seedlings. The experiment was done in a completely Randomized Design. Our experimental design was included10 random attendance namely: soaking of Ammodendron persicum seeds in gibberellic acid (300ppm) for 48 hr, seed scratching scarification with acid in two interval times of 20&30 min, incorporation of later with sand paper scratching scarification, seed scratching with gibberellic acid (300ppm) and time period of 48hr Ammodendron persicum seed sand papering combined with gibberellic acid soaking, wetting Ammodendron persicum seeds with high temperature water (80°C) for 5&10 min then scratching them by sand paper and also using distillated water as control treatment evidence. Experimental results showed, 30 minutes sulfuric acid soaking combined with sand papering can increase germination to 90% of the laboratory. In addition, seed scratching with gibberellic acid (300ppm) and time period of 48hr Ammodendron persicum seed sand papering combined, wetting Ammodendron persicum seeds with high-temperature water (80°C) for 5&10 min, the percentage of germination, respectively, 45/15, 45/8, 17/10 and 68/28 respectively. Moreover, 30min high-density sulfuric acid caring improves Ammodendron persicum, seed vigor, the power of greenhouse and lab samples to 450 and 510 respectively. Finally, authors reported scratching and acid soaking combination as an efficient, caring method in this research.


Marzieh Hasani, Dr Mahmoud Reza Tadayon, Dr Majid Olia,
Volume 11, Issue 2 (3-2025)
Abstract

Extended abstract
Introduction: Germination and seedling emergence are among the most critical developmental stages in crop plants, significantly influencing subsequent growth stages in the field. These traits are directly linked to seed vigor and potential. The application of organic and biofertilizers can enhance seed and seedling vigor, enabling them to withstand environmental stresses such as drought and aridity. To investigate this, the effects of drought stress and fertilizer treatments on germination indices of two plant species—red bean (Phaseolus vulgaris L.) and jicama (Pachyrhizus erosus (L.) Urban)—were examined.
Materials and methods: The seeds of jicama and red bean (Goli variety) were subjected to four levels of drought stress (0, -6, -9, and -12 bar) and four fertilizer treatments: control (no fertilizer), optimal biological fertilizer (EM), optimal organic vinasse fertilizer, and a combination of biological and organic fertilizers (at optimal levels). The experiment was conducted as a factorial design in a completely randomized arrangement with three replications at the University of Shahrekord during the 2022-2023 growing season.
Results: The results revealed that the triple interaction of plant species, fertilizer treatments, and drought stress had a significant effect (p < 0.01) on germination percentage, germination rate, seed vigor index, shoot and radicle length, as well as fresh and dry weights of shoots and radicles. Under non-stressed conditions, the jicama species treated with combined fertilizers exhibited the highest mean values for germination percentage (100%), germination rate (42.48 seeds/day), seed vigor index (8.55), shoot length (5.66 cm), shoot fresh weight (0.694 g), shoot dry weight (0.125 g), radicle fresh weight (0.772 g), and radicle dry weight (0.109 g). Under moderate drought stress (−9 bar), the mean radicle length of jicama treated with combined fertilizers was 6.333 cm.
Conclusion: In this study, the application of fertilizer treatments enhanced greater tolerance to water stress conditions and reduced water potential, while drought stress treatments led to a decline in the examined traits in both bean species. However, the jicama species demonstrated superior drought stress tolerance compared to the red bean, making it a more suitable option for cultivation under drought stress conditions

Highlights:
  1. The novelty of jicama in Iran and the lack of information on the germination and growth characteristics of this plant.
  2. Due to the presence of rotenone in jicama seeds, there was no information on how this compound interacts with fertilizer treatments to affect seed germination traits—an aspect investigated in this study.
  3. Since jicama is a multipurpose plant with both seed and tuber uses, there was no available data on how its germination traits compare to those of a similar plant, such as beans.

Mehrab Yadegari,
Volume 11, Issue 2 (3-2025)
Abstract

Extended abstract
Introduction: Salvia sp. from Lamiaceae family is one of the important medicinal and pasture plants that have many uses in various medicinal and food industries. This study was performed to evaluate the effects of different hormonal treatments on germination in three species of Salvia including S. officinalis, S. leriifolia, and S. syriaca under different wet stratification periods.
Materials and Methods: This research was conducted in factorial layout experiment in a completely randomized design (CRD) with 3 replications and 2 separate repetitions in Herbal Research Institute, Shahrekord Branch, Islamic Azad University in 2024. Experimental treatments included wet chilling (0, 10, 20, 30 day in 4°C and RH=45%)  as first factor (A1-A4) and hormones inclusive (gibberellic acid (120ppm), benzyl adenine (10-4 M), kinetin (10-4 M), gibberellic acid (120 ppm) + benzyl adenine (10-4 M), gibberellic acid (120 ppm) + kinetin (10-4 M), benzyl adenine (10-4 M) + kinetin (10-4 M), gibberellic acid (120 ppm) + benzyl adenine (10-4 M)+ kinetin (10-4 M) and control) as second factor (B1-B8). Biochemical indices such as hydrogen peroxide, α-amylase and beta-1,3glucanase and germination indices such as dry weight of seedlings, length of seedlings, germination percentage, mean of germination time, seed vigour, and germination uniformity were measured.
Results: The use of wet chilling and hormonal treatments had significant effectiveness on germination characters. Gibberellic acid in the most of measured characters had beneficial and increasing effects. The most and lowest important measured germination characteristics and decreased time needed for germination. The highest and lowest amounts for germination characteristics such as seedling length (5.56-2.1 cm), seed vigor (5.1-0.94), seedling dry weight (45.35-19.84 mg), germination percentage (91.74-38.22), germination uniformity (21.03-3.57 day) and biochemical characters such as content of α-amylase (12.94-2.6 mM/g FW of seed), beta-1, 3 glucanase (10.79-2.83 mM/g FW of seed) and hydrogen peroxide (0.69-0.21 mM/g FW of seed) were recorded under wet chilling treatment for 20 day + gibberellic acid (120 ppm) + benzyl adenine (10-4 M) + kinetin (10-4 M) and treatment of without wet chilling + without hormonal treatment, respectively. Also, increasing wet chilling treatment duration, increased some evaluated characteristics, but most of them reached the highest values after 20 days wet chilling treatment. The amounts of evaluated characters in this treatment improved more than 100% in comparison to control seeds. It can be concluded that wet chilling (20 day) along with the use of gibberellic acid, benzyl adenine, and kinetin resulted in improved seed germination indices of Salvia spp. Results showed that in most of measured characteristics, the Salvia species have various amounts of biochemical and germination indices. Finally, the treatment of wet chilling (20 day) and use of gibberellic acid, benzyl adenine, and kinetin hormones, suggest to obtain of the best germination indices in seeds of Salvia spp. Germination percentage and most of the other measured characters in this research in S. officinalis were more than S. syriaca, and S. leriifolia, respectively.
Conclusion: Results showed that the most of measured characteristics of Salvia species have various amounts of biochemical and germination indices. Generally, wet chilling (20 day) with the use of gibberellic acid, benzyl adenine, and kinetin suggest to best seed germination of Salvia spp.

Highlights:
  1. Study the various periods of wet chilling and hormonal treatments on biochemical and germination indices of Salvia officinalis, S. leriifolia, S. syriaca.
Wet chilling (20 day) with the use of gibberellic acid, benzyl adenine, and kinetin suggest to best seed germination of Salvia spp.


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