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Sayyed Jalil Mirmahmood, Mohammad Javad Ahmadi-Lahijani, Yahya Emam,
Volume 1, Issue 2 ((Autumn & Winter) 2015)
Abstract

Osmotic stress is the most important of the abiotic stresses that lead to limitation of growth and yield in rapeseed. This study was conducted to examine the effect of cycocel seed priming on osmotic stress tolerance in germination and early growth stages of rapeseed, as a factorial experiment based on completely randomized design at Agriculture College of Shiraz University in 2012. The treatments included seed priming in three cycocel concentrations: 0 (without priming as control), 2.5 and 3.5 g L-1 and osmotic stress in five levels: 0 (as control), -0.3, -0.6, -0.9 and -1.2 MPa using polyethylene glycol. The results showed that osmotic stress was significantly reduced germination percentage (15.1%), germination rate (15.3%), radicle length (29.5%), plumule length (31.6%), radicle fresh weight (22.4%), plumule fresh weight (37.6%) and vigor index (39.9%). Furthermore, osmotic stress leads to increase radicle to plumule fresh weight and radicle to plumule length ratio as 6.8 and 24.3 percent, respectively. Seed priming with cycocel reduced the adverse effects of osmotic stress on measured parameters. Although, there was no positive effect on germination percentage and rate under osmotic and nonosmotic conditions for cycocel in both concentrations, but cycocel at 3.5 g L-1 appeared to have a greater positive effect than 2.5 g L-1. Considering positive impact of cycocel seed priming on the reduction adverse effects of osmotic stress further research on cycocel seed priming and osmotic stress tolerance is recommended under field conditions.


Seyyed Mohammad Seyyedi, Mohammad Khajeh-Hossieni, Parviz Rezvani Moghaddam, Hamid Shahandeh,
Volume 2, Issue 1 ((Spring and Summer) 2015)
Abstract

In order to investigate the effects of priming with a KH2Po4 solution on emergence and heterotrophic growth of black cumin (Nigella sativa L.) seedling, a series of experiments were conducted at the Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, in 2013 and 2014. A field experiment was conducted as factorial layout based on a randomized complete block design with four replications. The fertilizer resources (vermicompost (V)+Tiobacilus (T), micronized Sulfur (S)+T, V+S+T and control) and three levels of phosphorus (0, 30 and 60 kg.ha-1) were the first and second experimental factors, respectively. In the second experiment, selected treatments (in which showed emergence below 60% in the previous experiment) and seed priming (no priming, hydro priming and three levels of phosphorus (P)-priming as 100, 300 and 500 mM KH2Po4) were the first and second experimental treatments, respectively. Based on the results, the resources of soil amendment (V+T and V+S+T) significantly increased the emergence percentage of black seed (by 50 and 54%, respectively). In addition, seed reserve depletion percentage, seed reserve utilization efficiency and emergence percentage significantly increased by 300 mM KH2PO4 (by 9, 143 and 29%, respectively). There was a significant positive relationship between the weight of mobilized seed P and the emergence percentage of black seeds (R2= 0.90 **).


Hamid Sharifi, Mohammad Khajeh-Hosseini, Mohammad-Hassan Rashed-Mohassel,
Volume 2, Issue 1 ((Spring and Summer) 2015)
Abstract

Seeds of seven species of medicinal plants collected from the natural habitat in Lorestan province in summer 2011. Germination test carried out in a completely randomized design with four replications of 25 seeds in H2O. Species of Smyrnium cordifrolium, Kelussia odoratissima, Dorema aucheri and Ferulago angulata had no germination while Heracleum persicum, Bunium luristanicum and Falcaria vulgaris showed germination of 30, 96 and 97% respectively. Different treatments of breaking dormancy applied to the species with germination below 30% [moist-chilling for periods of 2, 4, 6, 8, 10 and 12 weeks, with two concentrations of 250 and 500 ppm of gibberellic acid, a combination treatment (gibberellic 250 ppm + 4 weeks moist-chilling and gibberellic acid 500 ppm + moist-chilling for 4 weeks) and potassium nitrate 2 g/l]. The results showed that moist-chilling was the most effective treatments to break seed dormancy of Heracleum persicum (6 weeks), Dorema aucheri (12 weeks), Kelussia odoratissima (12 weeks) and Ferulago angulata (12 weeks). Therefore, based on their reactions to the treatments, dormancy of Kelussia odoratissima and Ferulago angulata could be classified as deep physiological dormancy and species of Dorema aucheri and Heracleum persicum intermediate physiological dormancy type.


Hamid Sharifi, Morteza Goldani,
Volume 2, Issue 2 ((Autumn & Winter) 2016)
Abstract

Seed coat color as a perfect and simple index is effective in water absorption, seed dormancy and germination. The mustard plant produces seeds with different coat color. So, in order to investigate the effect of seed coat color on germination rate, the percentage of germination and dormancy of Mustard (Sinapis arvensis L.) seeds an experiment was carried out based on a completely randomized design with four replications. Treatments were included two types of seed coat color (brown and black) and dormancy breaking treatments (Control, Potassium nitrate 0.2%, GA3 250, GA3 500 ppm and one, two and three weeks prechilling). The results showed that the differences between germination indices traits in brown and black seeds were significant. So that, the germination rate and germination percent in black seed were greater than brown seeds, but the duration of dormancy in brown seeds was greater than black seed. One week prechilling treatment had the most effect on breaking dormancy. So that germination percentage and germination rate for black seed was 75% and 0.54 respectively and these amounts for brown seeds were 58% and 0/43 respectively. Potassium nitrate and GA3 (250 and 500 ppm) reduced germination rate and germination percent in both types of seeds. In addition, water absorption percent in brown seeds was more than black seeds. The final results showed that heteromorphic seeds of Mustard have variation in duration of dormancy, germination and water absorption percent.


Maryam Janalizadeh, Ahmad Nezami, Hamidreza Khazaie, Hassan Feizi, Morteza Goldani,
Volume 3, Issue 1 ((Spring and Summer) 2016)
Abstract

Priming of seeds by magnetic fields (magneto priming) is proposed as an ecological, useful and low-priced method for improvement of seed germination and plant emergence. In order to evaluate the germination behavior of sesame seeds affected by magnetic fields, an experiment was conducted as a completely randomized design with 22 treatments (non-exposure to magnetic fields (control) and 21 magneto priming treatments) with three replications at the college of agriculture, the Ferdowsi University of Mashhad in 2014. the seed of sesame put into a plastic bag bulky and was treated with different intensity of magnetic fields (25, 50, 75 and 100 mT) for several times (10, 20, 30, 60 and 120 minutes). For assessment of germination traits of sesame under constant magnetic field conditions, magnetic tapes with three mT strength were used in each Petri dish throughout the experiment. Results showed that magnetic fields had no significant effects on final germination percentage and mean germination time and all magnetic treatments except for 75 mT at 60 minutes indicated reducing effects on germination percentage in comparison to control. Priming of seeds with magnetic fields in 50 mT strength for 20 minutes led to the increment of germination rate compared to control treatment but all levels of 100mT treatment caused a reduction in germination rate than control. The most radicle length, seedling length and seedling vigor length index belong to 75 mT at 60 minutes treatment and the most seedling dry weight and seedling vigor weight index related to 100 mT for 20 minutes treatment. Ranking of treatments showed that exposure of seeds for one hour in 75 mT and 10 minutes in 25 mT magnetic fields strength had the best outcomes.


Almas Nemati, Hamid Sharifi, Mohammad Gerdakaneh, Zeynab Sharifi,
Volume 3, Issue 1 ((Spring and Summer) 2016)
Abstract

The seeds of two species (Citrulus colocynthis) and (Silybum mrianum) gathered from natural habitat located in the Koohdasht city (Lorestan province) and transferred to Kermanshah Agricultural and Natural Resources Research and Education Center in the summer of 2014. For each species, separate experiments in a completely randomized design with 14 treatments and 4 replications were done. Treatments included moist-chilling time (control, 10, 20, 30, 40, 50, 70 and 90 days at 4 °C), gibberellic acid concentration (200, 400, 600 and 800 PPM) and integrated treatment of gibberellic acid 400 ppm with moist-chilling for 30 days and 70 days. The results showed that for both species increasing of moist-chilling duration and concentration of gibberellic acid significantly increased germination percentage, germination rate and seed vigor index. The best breaking seed dormancy treatment was moist-chilling of 90 days for seeds of (Citruluus colocynthis) and gibberellic acid 800 ppm for (Silybum mrianum). The result showed that seeds of two species had the combination of physiological (related to seed inside inhibitors) and physical dormancy (related to hard coat).


Abdoljalil Yanegh, Mohammad Khajehosseini,
Volume 3, Issue 1 ((Spring and Summer) 2016)
Abstract

The need to identifying seeds with low vigor and poor emergence led to introduce of various seed vigor tests. The purpose of this experiment was to assess the ability of controlled deterioration test for evaluation of seed vigor and prediction of oilseed rape seed lots emergence in the field. Samples of 19 seed lots were obtained from nineteen farmers of Razavi and Northern Khorasan Provinces, which have been cultivated during 2009-2010 growth season, set to physiological germination test before and after controlled deterioration (CD). The estimating of seedling emergence percentage of each seed lot in the field calculated 30 days after sowing. The correlation between laboratory results before and after CD of seeds and field emergence showed that correlation of final germination, normal seedlings and mean germination time before CD with emergence in the field respectively with 0.072, 0.24 and 0.042 correlation coefficient, were not significant, but laboratory results after CD had significant correlation with emergence of seed lots in the field. As, final germination, normal seedlings and mean germination time respectively with 0.57, 0.51 and 0.49 correlation coefficient predict emergence percentage of seed lots in the field. The high correlation between laboratory results after controlled deteriorated test with the seedling emergence in the field indicated that the CD test is able to assess the field emergence of oilseed rape seed lots before sowing.


Seyyed Mahdi Javadzadeh, Parviz Rezvani Moghaddam, Mohammad Banayan-Aval, Javad Asili,
Volume 3, Issue 2 ((Autumn & Winter) 2017)
Abstract

Roselle is an important medicinal and industrial plant of the family of Malvaceae, and is planted in vast areas of Sistan and Baluchestan. In a laboratory study, the effect of varying temperatures on seed germination of Hibiscus sabdariffa was investigated and minimum, optimum and maximum temperatures for its germination were determined in a completely randomized design with four replications.  For this purpose, temperatures 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50°C were considered in each treatment. Cardinal temperatures for germination were determined consistent with three models (i.e., Intersected-lines Model, Five-Parameters Beta Model and Quadratic Polynomial Model). The traits measured were germination percentage, the speed of germination and mean germination time. The temperature effect on all the measured traits was significant. The results of the regression analysis showed that the best model in terms of cardinal point of this plant is the Five-Parameters Beta Model. Given the results of this model, the minimum and the optimal temperatures for the germination of Roselle are 4.04°C, and 29.83° C, respectively.
 


Mostafa Alinaghizadeh, Mohammad Khajeh-Hosseini, Seyed Ahmad Hosseini, Mohammad Hasan Rashed Mohasel,
Volume 3, Issue 2 ((Autumn & Winter) 2017)
Abstract

In order to study the seed germination behavior and dormancy breaking methods of three weed species (i.e., Chenopodium album, Convolvulus arvensis and Setariaviridis) of pistachio orchards in Rafsanjan, Iran, three separate factorial experiments (with 2 factors) were conducted based on a completely randomized design with four replications, at the Faculty of Agriculture, Vali-E-Asr University of Rafsanjan, Iran, in 2014. Weed seeds were collected from five different regions of Rafsanjan, such as Markazi, Anar, Koshkoiyeh, Kabotarkhan and Nogh. Dormancy breaking treatments for Chenopodium album involved distilled water (control), KNO3 (at 500 and 1000 ppm), chemical scarification by sulfuric acid (for 5 and 10 min), and cold stratification (for 1, 3 and 5 weeks). Treatments for Convolvulus arvensis involved distilled water (control), scarification by sandpaper, chemical scarification by sulfuric acid (20 and 30 min), and boiling water (for 15 and 30 min). Treatments for Setaria viridis involved distilled water (control), gibberellic acid (250, 500 and 1000 ppm), KNO3 (500 and 1000 ppm), and cold stratification (for 1, 3 and 5 weeks). The results showed that seed germination percentage (SGP) and mean germination time (MGT) of three weed species were significantly different among weed populations and dormancy breaking methods. For Chenopodium album, cold stratification of 5 weeks resulted in highest SGP (97%) in Nogh population. For Convolvulus arvensis and Setaria viridis, the highest SGP was obtained after scarification by sandpaper (98% in Kabotarkhan population) and using 1000 ppm gibberellic acid (60% in Kabotarkhan population), respectively. In addition, increasing the weight of 1000 seeds in the three weed species in question increased SGP.
 
 


Masume Hematifar, Ali Tehranifar, Hasan Akbari Bishe, Bahram Abedi,
Volume 4, Issue 2 ((Autumn & Winter) 2018)
Abstract

Given the medicinal and ornamental properties of Hawthorn (Crataegus spp.), and given that there are some problems in its propagation, which is due to the hard cover of the seeds and immature embryo, working out techniques that can facilitate the process of seeds’ germination is of great import. Thus, the present study sought to identify the best method for the purpose of breaking the seed dormancy of 8 species of native hawthorn of Iran. The study was carried out as factorial with a completely randomized design and had three replications. The first, second and third factors were, respectively species of Hawthorn in eight levels, sulfuric acid in three levels (half and an hour versus non-treated (control) and chilling treatment in three levels (90, 105 and 120 days, respectively). The results showed that the highest percentage of germination (32%) and germination rate (9.1 day-1) were obtained under the interaction of sulfuric acid treatment for one hour, followed by a 120-day chilling period in C.turkestanica, which had a significant difference with other treatments. It is concluded that hawthorn seeds have deep endocarp and physiological dormancy. The interaction of Sulfuric acid treatment and moist chilling play an important role in increasing the percentage and speed of germination of Hawthorn seeds.

Highlights:

  1.  Achieving the best way to break the dormancy of Hawthorn seeds in different species and genotypes.
  2. Shortening the seeds’ germination time.

Elnaz Mohamadian, Hormozdyar Kianmehr, Hojjat Ataei Somagh, Neda Azad Nafas Mahjor, Fatemeh Safari, Arezo Safarzadeh,
Volume 5, Issue 1 ((Spring and Summer) 2018)
Abstract

Extended abstract
 Introduction: Stevia is a perennial short day plant, belonging to the Asteraceae family. It is also called sugar leaf. Poor germination of this plant serves as a barrier for its planation on a large scale, which contributes to its scarcity and expensivenss as a medicinal herb. In many plants, seed germination is sensitive to salinity, which determines the survival of the plants in saline soils. High levels of soil salinity can significantly reduce germination and seedling growth due to the effects of high osmotic potential and ion toxicity. Jasmonates represent new plant growth regulators that play an important role in increasing the resistance of plants to environmental stresses, including salinity stress. Therefore, this experiment was conducted to study the effect of pre-treatment of seed with methyl jasmonate on germination indices and biochemical traits of stevia, as a medicinal herb, under salinity stress.
Materials and Methods: They study was conducted, adopting a completely randomized design with three replications in the year 2016 in the Professor Hassabi’s Laboratory of Plant Biology, Islamic Azad University, Islamshahr Branch. The factors were pre-treatment of methyl jasmonate in 5 levels (0, 2.5, 5, 10 and 15 μM) and salinity stress at 4 levels (0, 3, 6 and 9 dS m-1). At the end of the experiment, germination traits percentage and germination rate, mean germination time, germination value, seedling length, seedling index, total chlorophyll, proline, activity of the enzyme catalase, peroxidase and superoxide dismutase were measured.
Results: The results of the study showed that effects of salinity stress, methyl jasmonate and interaction between salinity and methyl jasmonate were significant on the germination percentage and germination rate, mean germination time, germination value, seedling index, total chlorophyll, proline and catalase enzyme activity. Seed priming with 5 μM methyl jasmonate at salinity level with electrical conductivity of zero ds/m, had the highest germination percentage and rate, germination value, seed vigor index, and total chlorophyll content. Increases in salt stress and methyl jasmonate increased the activity of catalase enzyme. Salinity reduced germination index and seedling stoichiation and increased activity of peroxidase and superoxide dismutase enzymes. However, seed priming with methyl jasmonate improved seed germination through germination percentage, germination rate and seed vigor index and moderated the effects of salt stress.
Conclusions: Given the results of this study, it could be said that methyl jasmonate, as a potent inhibitor, can reduce the negative effects of salinity and by increasing germination indices such as germination percentage and germination rate, it can be effective in improving the growth of Stevia. Of course, further research can produce more definitive results.
 
 
Highlights:
  1. Salinity had a negative effect whereas methyl jasmonate had a positive effect on germination indices and activity of antioxidant enzymes of Stevia seeds.
  2. Application of 5 μM of methyl jasmonate, as a pre-treatment, can be effective in improving the growth of the stevia plant and reducing the negative effects of salinity.

Hosein Sarani, Ebrahim Izadi, Ali Ghanbari, Ali Rahemi,
Volume 6, Issue 1 ((Spring and Summer) 2019)
Abstract



Extended Abstract
Introduction: In recent years, Japanese morning glory has been recognized as a new weed in some soybean cultivation areas in the Province of Golestan. Japanese morning glory, an annual herbaceous plant, belongs to Convolvulaceae family. Germination is the first step in the competitiveness of a weed in an ecological niche. Among the factors influencing seed germination, temperature and light are the most important environmental factors. The relationship between temperature and germination rate is mainly determined by nonlinear regression, and various models such as dent-like, segmented, beta, and second-order major models are used for this purpose. In this study, we examined the aspects of germination biology of this weed under the influence of temperature and light.
Materials and Methods: In order to investigate the effect of temperature and light on germination of Japanese morning glory, two separate experiments were conducted. Treatments included constant temperature at 7 levels (10, 15, 20, 25, 30, 35, 40) in the first experiment and alternating temperature at 6 levels (30/25, 10/15, 30/20, 35/25, 40/30, 45/35) and light conditions (14 hours of brightness 250 μmoles/m-2-sec-1) and darkness in the second experiment based on a completely randomized design with four replications. The number of germinated seeds was taken up to 4 days after stopping germination every day. Percentage and speed of germination and time reaching 50% germination were calculated. Three models of dent-like, segmented lines and beta were used to determine the cardinal temperature between the temperature and germination rate.
Results: The results showed that temperature had a significant effect on percentage, speed and time taken to reach 50% (D50) of germination of Japanese morning glory. The highest percentage of germination (95%) and germination rate (19.80 seeds per day) were observed in the alternating temperature of 20/30 ° C treatment, respectively. The lowest percentage of germination (83.33%) was observed at alternating temperatures 25/35 °C, and the lowest germination rate (15.10 seeds per day) was observed at 10-20 °C. The segmented lines, dent-like and beta were best fit based on the highest R2adj 0.95, 0.96 and 0.95, respectively. Light had no significant effect on germination, so that germination occurred under both light and dark conditions. According to the results, Japanese morning glory is able to germinate at a wide range of constant and alternating temperatures, although germination is faster at warmer temperatures. On the other hand, the lack of light for germination is another advantage that increases germination, competition, and expansion in agronomic environments.
Conclusion: The findings of the present study suggest that the highest percentage of germination and rate of germination were observed in alternating temperatures of 20/30 °C respectively. Among the nonlinear regression models, the dent-like model represented the best model for describing the germination rate against the temperature in Japanese morning glory. It seems that this weed has better germination at warmer temperatures. Probably from mid-spring following warmer weather, and upon the availability of water, this weed is in a good situation to germinate and compete. It was also found that light had no significant effect on the germination of this weed.

Highlights:
  1. Non-photoblastic seeds
  2. Superiority of dent-like model for predicting germination of Japanese morning glory

Mahboubeh Hajiabbasi, Reza Tavakkol Afshari, Alireza Abbasi, Reza Kamaei,
Volume 6, Issue 2 ((Autumn & Winter) 2020)
Abstract



Extended Abstract
Introduction: Soybean (Glycine max (L.) Merrill) is the primary source of vegetable oil. Even in desirable conditions, soybean seeds lose their viability in long term storage. Many factors contribute to seed deterioration, including genetic factors, mechanical damage, relative humidity, storage temperature, seed moisture content, existence of microflora, and seed maturity, which reduce seed quality and make seeds unfit for cultivation purposes.
Materials and Methods: In order to investigate the effects of seed deterioration on seed germination and also the effects of salicylic acid and ethylene on the improvement of deteriorated seeds of G. max., accelerated aging test for 0, 6 and 10 days and natural aging test for 6 months were conducted. After aging conditions, seeds were imbibed with 50 µM salicylic acid and 10 µM ACC (precursor of ethylene) for 6 hours at 25 °C. In addition, after natural and accelerated aging tests, a bunch of seeds was used without any hormonal treatment (i.e., dry seeds) as control seeds. The seeds’ germination percentage, total sugar, fructose, and glucose were investigated. Moreover, the gene expression of GAI1 and LOX1 was measured on dry seeds and under imbibition of water, salicylic acid and ACC at 6, 12 hours using Q-RT-PCR method.
Results: The germination results showed that increasing number of aging days led to a decrease in germination. Total sugar content in seeds aged for 6 days did not have a significant difference, as compared with non-aged seeds. However, total sugar content in seeds aged for 10 days was significantly higher than non-aged seeds. Increasing accelerated aging levels from 0 days to 10 days led to increases in glucose and fructose contents in dry seeds. In addition, genes exhibited different expressions in different days and hours. Increasing aging from 0 days to 10 days led to increases in GAI1 gene expression. Moreover, LOX2 expression increased in accelerated aging from 0 to 6 days. LOX2 gene expression in naturally dried aged seeds also increased and was higher than that in non-aged seeds. SA and ACC had different effects on measured values.
Conclusion: In general, it can be concluded that the deterioration of seed quality and vigor result from numerous degradation processes and disruption in seeds’ physiological activity. This study showed that aging is associated with an increase in total sugar, glucose and fructose levels. In addition, the expression of the genes involved in the germination is also affected. Increases in LOX2 gene expression were observed in both accelerated aging and natural aging pathways. GAI1 gene expression increased in accelerated aging. However, in normal aging, it decreased.
 

Highlights:
  1. Identifying the role of LOX2 and GAL1 genes in soybean seed deterioration.
  2. Investigating seeds’ physiological responses under natural and laboratory aging conditions.

Sajad Mijani, Mehdi Rastgoo, Ali Ghanbari, Mehdi Nassiri Mahallati,
Volume 7, Issue 2 ((Autumn & Winter) 2021)
Abstract

Extended Abstract
Introduction: Tubers are considered as the most important vegetative organs in reproduction of purple nutsedge, as one of the most troublesome weeds worldwide. Therefore, it is great of importance to investigate the properties of the tuber response to the surrounding environment such as absorption and loss of water. Water uptake is the first step in the sprouting process, though the pattern of water uptake by purple nutsedge tubers has not been documented. Loss of water in tubers is one of the potent factors in reducing their ability to sprouting. Three separate experiments were carried out to investigate the absorption and loss of water content of purple nutsedge tubers.
Material and Methods: In the first experiment, the tubers were placed in a water bath at temperatures of 10, 20, 30, and 40 ° C. Then, the weight of the tubers was measured at different times (24 till 3600 minutes). The water uptake percentage of tubers at different temperatures was studied by fitting the Peleg model. In the second experiment, the initiation day of sprouting was investigated at constant temperatures of 10, 20, 30, and 40 ° C. In the third experiment, water loss and sprouting percentage of tubers were evaluated in two conditions refrigerator (4° C) and room (22 to 25 ° C).
Results: The results showed that the initial water content of tubers was 42% and absorbed 10% extra water after being immersed in water. The water uptake behavior was based on the Peleg model at two stages: (1) rapid uptake (less than 420 minutes (7 hours), and (2) a low uptake with a gentle slope afterward. In the Peleg model, the parameters K1 (minutes *.%weight -1) and K2 (%-1) are water absorption rate and water absorption capacity, respectively. The K1 parameter was negatively against temperature. The highest and lowest values were 49.56 and 28.55 at 10 and 40 ° C, respectively. On the other hand, the trend of the K2 was constant (0.1) at 10-30 °C but was 0.08 at 40 °C. The two-parameter Hyperbola model was superior to the Peleg and predicts the highest water absorption and time to 50 percent water absorption parameters. The results showed that sprouting of purple nutsedge tubers at 10, 20, 30, and 40 °C occurred after 14.44, 6.57, 3.24, and 3.12 days, respectively. Keeping the tubers in the room (22-25 °C) and refrigerator (4 °C), sprouting stopped after 3 and 9 months, respectively. The time required for 50% reduction of sprouting in the room and refrigerator was estimated to be 1.3 months (39 days) and 5.12 months (154 days), respectively. The time required for 50% loss weight of tubers in the room and refrigerator was 1.981 months (59 days) and about 6 months (180 days), respectively. Overall, weight loss (water loss) up 11.85%, resulted in 50% reduction in tuber sprouting.
Conclusion: Maximum water uptake in tubers occurred in less than 420 minutes (seven hours) at all temperatures. Slow sprouting in tubers at low temperatures is not associated with an obstacle in water absorption. Tubers lost half of their sprouting ability by losing water about 12%. On the other hand, the results show that the tubers at cool temperatures (4 °C) lose their water and sprouting capacity less than the ambient temperature (22 to 25 °C).

Highlights:
1- Determination of water absorption pattern on purple nutsedge tubers.
2- Effect of storage location in reducing water and sprouting ability of purple nutsedge tubers.


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