Search published articles


Showing 3 results for karimi

Abbas Dehshiri, Mojtaba Karimi, Mohammadhasan Asareh, Aidin Hamidi, Zahra Karbalaeiharoftah, Fazlolla Safikhani,
Volume 8, Issue 1 ((Spring and Summer) 2021)
Abstract

Extended abstract
Introduction: Small size of Satureja (Satureja hortensis L.) seed causes many problems in mechanized cultivation and its seed consumption is increased significantly.  The approach discussed in the present study is the seed coating of Satureja by several matters and selecting the suitable method as well as investigation of adding hormonal and nutritional substances to the selected coating material for better emergence and the elimination of problem arise as a result of coating.
Materials and Methods: Purified Satureja seeds (97%) were prepared and poured into the coating machine at a specified amount. First, 1% solution of benomyl fungicide was sprayed on the seeds. Then some coating powder was sprayed on the spinning wet seeds inside the machine. Then some of the adhesive solution was sprayed on it and again the coating powder was added and this was repeated several times to create a suitable form for the coated seeds. This was repeated using different materials and among these coatings powder, vermiculite and perlite provided better coating on Satureja seed. Then, different materials including gibberellin, BAP, cytokinin, thiourea (CH4N2S) and potassium nitrate (KNO3), phosphorus from H2PO4 source and manganese sulfate (MnSO4) were used to the emergence of the enlarged seeds and also, the emergence of these seeds were evaluated in the greenhouse.
Results: Initial results showed that vermiculite and perlite provided better cover for Satureja compared with the other coating materials. Investigating the emergence of perlite-coated seeds in the greenhouse showed that gibberellin (all concentrations) and kinetin (500 mg/l) present in the seed coat improved the Satureja seed emergence under greenhouse condition. Also, it was found that Thiourea (700 and 7000 mg/l), potassium nitrate (7, 70, 700 mg/l), phosphorous (9 kg/100 kg seed) and manganese sulphate (2 and 4 mg/l) present in the coat significantly increased the Satureja seed emergence and led to improved seedling growth.
Conclusion: Perlite for the purpose of seed pelleting of Satureja showed good results and the use of gibberellin (at all concentrations) and quintine (500 mg/l) in the seed pelleting improved germination of pelleted Satureja seed.
 

Highlights:
1- Technical knowledge of Satureja seed pelleting was obtained.
2- The suitable material to improve the emergence of pelleted Satureja seed was introduced.

Ahmad Zare, Fatemeh Deris, Zahra Karimi,
Volume 8, Issue 1 ((Spring and Summer) 2021)
Abstract

Extended Abstract

Introduction: Notobasis (Syrian Thistle (has been introduced as a weed - medicinal plant. In Khuzestan province, the presence of Notobasis is abundant in cereal fields, especially field margins. For successful weed control, knowledge of weed biology and ecology (temperature, salinity and drought stresses) plays a key role in population dynamics of weeds and weed management. Therefore, the study aimed to evaluate Syrian Thistle response to temperature, salinity and drought, as well as to determine the cardinal temperature based on segmented, beta-four-parameter and dent-like models.

Materials and Methods: To investigate the effect of temperature (5, 10, 15, 20, 25, 30, 35 and 40 °C), salinity (zero, 50, 100, 150, 200, 250 and 300 mM) and drought stress (zero, 2) -0.0, -0.4, -0.6, -0.8, -1, -1.2 and -1.4 MPa). Three separate experiments were conducted in 2019 at agricultural science and natural resources university of Khuzestan at the laboratory of weed science with 6 replications. In each Petri dish, 25 seeds were placed and 7 ml of solution was added. In salinity and drought stresses experiments, sodium chloride solution and polyethylene glycol 6000 were used.
Results: Germination of Notobasis at temperatures of 5-30°C was more than 90%, and germination percent decreased to 24% as the temperature increased to 35°C, Based on three models segmented, beta 4 parameter and Dent-like, the Base temperature was estimated 2.95, 2.01 and 0.67°C respectively. Also, the optimum temperature in two models (segmented and beta parameter) was obtained 22.26 and 23.40°C respectively. Ceiling temperature was predicted 40.57, 39.75 and 40.03°C in three models (segmented, beta 4 parameter and Dent- like). Salinity required to reduce 50% of germination percentage, germination rate, seedling length and seedling fresh weight were 152, 85, 151 and 127 mM. 50% reduction of traits such as germination percent, germination rate, seedling length and seedling fresh weight were -0.81, -0.41, -0.43 and -0.45 MPa of drought stress respectively.
Conclusion: The results indicated that Notobasis had germination in a wide range of temperatures (5-35°C). In response to environmental stress, Notobasis have been identified as a weed resistant to salinity and drought stresses. Therefore, the presence of this weed in saline fields and arid areas is not unexpected.

Highlights:
1-To determine of cardinal temperature of Notobasis based on different models.
2-To evaluate germination ecology of Notobasis to environmental factors (temperature, salinity and drought stress) as the first report.


Enayat Rezvani Khorshidi, Mohammad Reza Jazayeri, Leila Sadeghi, Mohammad Rahmani, Farshid Hasani, Bita Oskoee, Seied Hosein Jamali, Amirali Karimi,
Volume 10, Issue 1 ((Spring and Summer) 2023)
Abstract

Extended Abstract:
 Introduction: Production of high-quality seeds to stabilize crop yield is an important challenge for breeders. One of the most important answers to this challenge is to clarify the molecular mechanisms associated with seed vigor characteristics. Functional proteins of Cupin superfamily are among the molecules in signaling pathway. Previous research has shown that in maize, a storage protein similar to the functional Cupin superfamily protein called ZmGLP is effective in seed germination. However, in the previous experiments, suitable indicators were not used to assess seed vigor and its relationship with field establishment. So, it is needed to study the performance of ZmGLP in predicting field emergence to complete the previous research.
 Materials and Methods: An experiment was performed on 14 samples of commercial inbred maize lines. In this experiment, in addition to the laboratory evaluation of seed germination, field indices of physiological seed quality including the percentage of seedling emergence in the field, time to 50% seedling emergence, time to 90% seedling emergence, seedling dry weight, seedling height and coefficient of variation of seedling height was also assessed. In the polymerase chain reaction, two pairs of primers (CF / CR primers and IDF / IDR primers) were used to identify the DNA sequence of the Cupin.
Results: The results show that the seeds were different in terms of physiological quality. The lowest percentage of germination in laboratory was related to K1264/1, while the lowest physiological quality of seeds in field indices was observed in K1263/17. The molecular test confirmed the presence of the desired allele at the InDel9 site of vigor-related genes in the three samples of B73, K1264/1, and K1264/5-1, but no amplification band of the InDel9 site was observed in all K1263/17 seed samples. Due to the fact that line K1264/1, which had the lowest germination percentage in the laboratory, had an amplification band at this related site to vigor, it is not enough to rely on the results of the laboratory germination test to investigate the relationship between this gene and seed vigor. The field emergence test and seed vigor test that have a good prediction of field emergence must be used in these studies.
Conclusions: According to the results of this experiment, molecular tests with functional markers based on Indel9 can be used to accelerate the evaluation of vigor, especially when the breeder is breeding a new line or hybrid. It is a useful, rapid, and effective molecular method to predict seed emergence in the field and screen the lines to ensure the genetic strength of the germination of the lines, especially in the temperate germplasms of corn. Finally, it is necessary to determine the threshold of low vigor during seed quality investigation in different cultivars, and relationship between the presence or absence InDel9 site should be considered in future research.

Highlights:
1- The feasibility of using molecular markers to determine the seed vigor of corn lines in the field was studied and optimized for the first time.
2- The results of physiological quality assessment of seeds in the field for the studies related to the relationship between molecular markers and seed vigor were exploited for the first time.
3- The Indel9 site and molecular markers related to seed vigor in the field were introduced.


Page 1 from 1     

© 2024 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb


This work is licensed under a Creative Commons Attribution 4.0 International License.