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Showing 43 results for Germination Percentage
Bijan Shadkam, Mohammed Hussain Gharineh, Amin Lotfi Jalalabadi, Seyed Amir Mousavi,
Volume 10, Issue 1 (9-2023)
Volume 10, Issue 1 (9-2023)
Abstract
Extended Abstract
Introduction: Salinity stress is one of the important damaging factors for seed germination which interrupts the uptake of some nutrients by lowering soil water potential and causing ion toxicity due to salt accumulation inside the seeds. Since hyssop seeds are sensitive to salinity stress in the early stages of seed germination, it is necessary to investigate seed enhancement methods to improve seed germination in this species under saline conditions. In this research, the effects of seed priming using iron were studied on hyssop seed germination characteristics under salinity stress conditions.
Material and Methods: This research was conducted as two separate factorial experiments in complete random design with three replications. The treatments included five levels of iron nano-chelate and micro-chelate (zero, 50, 100, 200, and 400 mg /l) and six levels of salinity (zero, 50, 100, 150, 200, and 250 mM) and two priming durations. It included 12 and 24-hours.
Results: The results showed that with the increase in salinity, the germination characteristics declined so at 200 mM of salinity, seedling growth declined significantly and ceased completely at 250 mM level. Application of iron micro-chelate under non-stress conditions did not have any positive effect on hyssop germination, and the use of distilled water for 12 hours worked better than iron nano-chelate. No significant difference was observed among the different concentrations in micro-iron treatment. Application of nano and micro chelate at 100 and 150 mM salinity stress had a stronger effect so that nano iron chelate concentrations of 200 mg/L for 12 hours and 100 and 200 mg per liter concentrations of iron micro chelate had the highest germination, seedling growth, and seed germination in 24 hours. The amount of soluble protein and peroxidase and catalase activity increased at 50 mg/L salinity but decreased at higher salinities. Also, pretreatment of seeds with an iron micro-chelate and nano iron chelate concentration of 200 mg/liter intensified this process.
Conclusion: Conclusion: The results of this study showed that seed germination of hyssop is highly declined due to salinity stress, so the seedlings were unable to grow at a salinity concentration of 200 mg/L. Moreover, germination does not occur at 250 mg/liter salinity. Also, seed pretreatment of this plant is not justifiable under non-stress and mild stress conditions, and pretreatment with iron chelate had negative effects and was ineffective at best. However, iron chelate application at high salinity levels- especially in the nanoscale with a concentration of 200 mg/liter for 12 hours- is effective in reducing the effect of salinity.
Highlights:
1- The role of the iron element was investigated by seed pretreatment method to alleviate the negative effects of salinity stress on hyssop seed germination characteristics.
2- The nanotechnology used in the production of iron fertilizer and its effect on improving the germination characteristics of hyssop under salinity stress were investigated.
Introduction: Salinity stress is one of the important damaging factors for seed germination which interrupts the uptake of some nutrients by lowering soil water potential and causing ion toxicity due to salt accumulation inside the seeds. Since hyssop seeds are sensitive to salinity stress in the early stages of seed germination, it is necessary to investigate seed enhancement methods to improve seed germination in this species under saline conditions. In this research, the effects of seed priming using iron were studied on hyssop seed germination characteristics under salinity stress conditions.
Material and Methods: This research was conducted as two separate factorial experiments in complete random design with three replications. The treatments included five levels of iron nano-chelate and micro-chelate (zero, 50, 100, 200, and 400 mg /l) and six levels of salinity (zero, 50, 100, 150, 200, and 250 mM) and two priming durations. It included 12 and 24-hours.
Results: The results showed that with the increase in salinity, the germination characteristics declined so at 200 mM of salinity, seedling growth declined significantly and ceased completely at 250 mM level. Application of iron micro-chelate under non-stress conditions did not have any positive effect on hyssop germination, and the use of distilled water for 12 hours worked better than iron nano-chelate. No significant difference was observed among the different concentrations in micro-iron treatment. Application of nano and micro chelate at 100 and 150 mM salinity stress had a stronger effect so that nano iron chelate concentrations of 200 mg/L for 12 hours and 100 and 200 mg per liter concentrations of iron micro chelate had the highest germination, seedling growth, and seed germination in 24 hours. The amount of soluble protein and peroxidase and catalase activity increased at 50 mg/L salinity but decreased at higher salinities. Also, pretreatment of seeds with an iron micro-chelate and nano iron chelate concentration of 200 mg/liter intensified this process.
Conclusion: Conclusion: The results of this study showed that seed germination of hyssop is highly declined due to salinity stress, so the seedlings were unable to grow at a salinity concentration of 200 mg/L. Moreover, germination does not occur at 250 mg/liter salinity. Also, seed pretreatment of this plant is not justifiable under non-stress and mild stress conditions, and pretreatment with iron chelate had negative effects and was ineffective at best. However, iron chelate application at high salinity levels- especially in the nanoscale with a concentration of 200 mg/liter for 12 hours- is effective in reducing the effect of salinity.
Highlights:
1- The role of the iron element was investigated by seed pretreatment method to alleviate the negative effects of salinity stress on hyssop seed germination characteristics.
2- The nanotechnology used in the production of iron fertilizer and its effect on improving the germination characteristics of hyssop under salinity stress were investigated.
Fatemeh Mousavi Nadar, Hamzeh Amiri,
Volume 11, Issue 1 (9-2024)
Volume 11, Issue 1 (9-2024)
Abstract
Extended abstract
Introduction: Allelopathy is the same as chemical warfare among plants. Today, global human efforts in agriculture are allocated towards reducing chemical pesticides and introducing new biological methods to control weeds. One of the proposed solutions for weed management is isolating allelochemical compounds from plants and using them as natural herbicides.
Materials and Methods: The present study was conducted to investigate some germination and biochemical properties of the weeds Taraxacum officinale, Achillea millefolium, Rumex acetosa, Althaea officinalis, and Amaranthus retroflexus As affectedby aqueous extract concentrations from the leaves of Thuja orientalis. The experiment was carried out in a completely randomized design with three replications. Aqueous leaf extracts of Thuja orientalis were used at concentrations of 0, 5%, 10%, 20% and 30%.
Results: The results showed that the aqueous leaf extract of Thuja orientalis had a significant effect on all the studied traits, and the highest inhibitory effect was observed at a concentration of 30% of the extract. In Taraxacum officinale, Achillea millefolium, Rumex acetosa, Althaea officinalis, and Amaranthus retroflexus, the germination percentage (93.33%, 88.55%, 48.3.%, 27.36%, and 40.5%), germination rate (100%, 76.36%, 39.28%, 71.72%, and 57.47%), germination coefficient (100%, 77.5%, 39.56%, 70.9%, and 51.47 %), germination index (57.2%, 72.6%, 84.61%, 72.5%, and 85.6%), peak value (67.9%, 48.7%, 61.4%, 70.9%, and 57%), germination value (43.2%, 39%, 46.9%, 53%, and 59.8%), daily germination speed (100%, 77.5%, 39.56%, 70.9% and 56.02%), seed vigor index (69.9%, 92.7%, 97.5%, 93.2%, and 92.1%), and relative water content (53.1%, 38.35%, 35.37%, 37.65%, and 47.1%) decreased compared with the control, while the mean germination time (51.6%, 71.6%, 50.7%, 60.3%, and 60.5%) and ion leakage (24.23%, 15%, 18.33%, 17.3% and 20.23%) increased compared with the control. The results of this study showed that the seeds of Taraxacum officinale were more sensitive to the inhibitory effects of the aqueous leaf extract of Thuja orientalis compared with the seeds of other studied weeds.
Conclusions: Considering the presence of monoterpene compounds such as alpha-pinene, sabinene, beta-phellandrene, and delta-carene, as well as phenolic compounds in Thuja orientalis, it seems that the allelopathic effects of this plant are mainly due to the presence of these secondary metabolites. The significant allelopathic effects of the aqueous leaf extract of Thuja orientalis at high concentrations can make it a potentially effective and environmentally friendly tool in controlling the studied weed species (Taraxacum officinale, Achillea millefolium, Rumex acetosa, Althaea officinalis, Amaranthus retroflexus) in this research.
Highlights:
Introduction: Allelopathy is the same as chemical warfare among plants. Today, global human efforts in agriculture are allocated towards reducing chemical pesticides and introducing new biological methods to control weeds. One of the proposed solutions for weed management is isolating allelochemical compounds from plants and using them as natural herbicides.
Materials and Methods: The present study was conducted to investigate some germination and biochemical properties of the weeds Taraxacum officinale, Achillea millefolium, Rumex acetosa, Althaea officinalis, and Amaranthus retroflexus As affectedby aqueous extract concentrations from the leaves of Thuja orientalis. The experiment was carried out in a completely randomized design with three replications. Aqueous leaf extracts of Thuja orientalis were used at concentrations of 0, 5%, 10%, 20% and 30%.
Results: The results showed that the aqueous leaf extract of Thuja orientalis had a significant effect on all the studied traits, and the highest inhibitory effect was observed at a concentration of 30% of the extract. In Taraxacum officinale, Achillea millefolium, Rumex acetosa, Althaea officinalis, and Amaranthus retroflexus, the germination percentage (93.33%, 88.55%, 48.3.%, 27.36%, and 40.5%), germination rate (100%, 76.36%, 39.28%, 71.72%, and 57.47%), germination coefficient (100%, 77.5%, 39.56%, 70.9%, and 51.47 %), germination index (57.2%, 72.6%, 84.61%, 72.5%, and 85.6%), peak value (67.9%, 48.7%, 61.4%, 70.9%, and 57%), germination value (43.2%, 39%, 46.9%, 53%, and 59.8%), daily germination speed (100%, 77.5%, 39.56%, 70.9% and 56.02%), seed vigor index (69.9%, 92.7%, 97.5%, 93.2%, and 92.1%), and relative water content (53.1%, 38.35%, 35.37%, 37.65%, and 47.1%) decreased compared with the control, while the mean germination time (51.6%, 71.6%, 50.7%, 60.3%, and 60.5%) and ion leakage (24.23%, 15%, 18.33%, 17.3% and 20.23%) increased compared with the control. The results of this study showed that the seeds of Taraxacum officinale were more sensitive to the inhibitory effects of the aqueous leaf extract of Thuja orientalis compared with the seeds of other studied weeds.
Conclusions: Considering the presence of monoterpene compounds such as alpha-pinene, sabinene, beta-phellandrene, and delta-carene, as well as phenolic compounds in Thuja orientalis, it seems that the allelopathic effects of this plant are mainly due to the presence of these secondary metabolites. The significant allelopathic effects of the aqueous leaf extract of Thuja orientalis at high concentrations can make it a potentially effective and environmentally friendly tool in controlling the studied weed species (Taraxacum officinale, Achillea millefolium, Rumex acetosa, Althaea officinalis, Amaranthus retroflexus) in this research.
Highlights:
- Leaf extract of Thuja orientalis has significant allelopathic effects on seed germination of the studied plants.
- Among the studied seeds, the seeds of the Taraxacum officinale plant are more sensitive to the aqueous extract of Thuja orientalis compared with others.
- The aqueous extract of Thuja orientalis leaves led to undesirable effects on the growth and seed germination of the studied plants by damaging cellular membranes.
Kamran Gharehbeygi Tavabea, Hamidreza Balouchi, Mohsen Movahhedi Dehnavi, Ali Moradi, Fatemeh Ebrahimi,
Volume 11, Issue 2 (3-2025)
Volume 11, Issue 2 (3-2025)
Abstract
Extended abstract
Introduction: Poor seedling vigor is one of the major challenges in agriculture, as it reduces seed germination capacity and seedling establishment, directly impacting plant establishment and yield. The use of seed priming methods with chemicals and growth regulators can serve as an effective strategy to enhance seedling vigor and improve biochemical traits and seed germination. In this study, the enhancement of sweet corn seedling vigor through priming with ellagic acid, gibberellin, and potassium nitrate was investigated under accelerated aging conditions.
Materials and Methods: A factorial experiment was conducted in the Seed Technology Laboratory of the Faculty of Agriculture at Yasouj University in 2024, based on a completely randomized design with two factors. The first factor was accelerated seed aging at two levels (aged and non-aged), and the second factor was seed priming at eight levels (no prime, hydropriming, ellagic acid at two concentrations (25 and 100 mg/l), gibberellin at two concentrations (50 and 150 mg/l), and potassium nitrate at two concentrations (15 and 60 mg/l)). Biochemical traits and seed germination characteristics were measured ultimately.
Results: The findings of this study revealed that seed deterioration significantly reduced germination characteristics, including germination percentage and rate, shoot length, root length, and seedling vigor index (length and weight). Additionally, biochemical indices such as soluble sugar content and α-amylase enzyme activity were negatively affected by aging. On the other hand, seed deterioration increased proline content, malondialdehyde levels, and peroxidase enzyme activity, indicating heightened oxidative stress under aging conditions. Priming of deterioration seeds with ellagic acid, gibberellin, and potassium nitrate positively influenced germination characteristics and biochemical indices. Specifically, concentrations of 100 mg/l ellagic acid, 150 mg/l gibberellin, and 15 mg/l potassium nitrate were the most effective pretreatments.
Conclusion: The results of this research demonstrated that pretreatment of deteriorated Basin sweet corn seeds with ellagic acid, gibberellin, and potassium nitrate significantly improved germination and biochemical indices. These treatments mitigated the negative effects of seed aging, enhancing seedling vigor and establishment. Therefore, these priming can be recommended as effective methods to improve the quality of Basin sweet corn seeds under various agricultural conditions.
Highlights:
Introduction: Poor seedling vigor is one of the major challenges in agriculture, as it reduces seed germination capacity and seedling establishment, directly impacting plant establishment and yield. The use of seed priming methods with chemicals and growth regulators can serve as an effective strategy to enhance seedling vigor and improve biochemical traits and seed germination. In this study, the enhancement of sweet corn seedling vigor through priming with ellagic acid, gibberellin, and potassium nitrate was investigated under accelerated aging conditions.
Materials and Methods: A factorial experiment was conducted in the Seed Technology Laboratory of the Faculty of Agriculture at Yasouj University in 2024, based on a completely randomized design with two factors. The first factor was accelerated seed aging at two levels (aged and non-aged), and the second factor was seed priming at eight levels (no prime, hydropriming, ellagic acid at two concentrations (25 and 100 mg/l), gibberellin at two concentrations (50 and 150 mg/l), and potassium nitrate at two concentrations (15 and 60 mg/l)). Biochemical traits and seed germination characteristics were measured ultimately.
Results: The findings of this study revealed that seed deterioration significantly reduced germination characteristics, including germination percentage and rate, shoot length, root length, and seedling vigor index (length and weight). Additionally, biochemical indices such as soluble sugar content and α-amylase enzyme activity were negatively affected by aging. On the other hand, seed deterioration increased proline content, malondialdehyde levels, and peroxidase enzyme activity, indicating heightened oxidative stress under aging conditions. Priming of deterioration seeds with ellagic acid, gibberellin, and potassium nitrate positively influenced germination characteristics and biochemical indices. Specifically, concentrations of 100 mg/l ellagic acid, 150 mg/l gibberellin, and 15 mg/l potassium nitrate were the most effective pretreatments.
Conclusion: The results of this research demonstrated that pretreatment of deteriorated Basin sweet corn seeds with ellagic acid, gibberellin, and potassium nitrate significantly improved germination and biochemical indices. These treatments mitigated the negative effects of seed aging, enhancing seedling vigor and establishment. Therefore, these priming can be recommended as effective methods to improve the quality of Basin sweet corn seeds under various agricultural conditions.
Highlights:
- Seed deterioration leads to an increase in biochemical indicators such as seed proline content, peroxidase enzyme activity, and seed malondialdehyde content.
- The vigor of Basin sweet corn seeds is improved through pretreatment with gibberellin and ellagic acid.
- The effect of priming before and after artificial deterioration on germination and seed vigor of sweet corn cultivar Basin was compared and investigated.
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Seed Science and Technology, Seed Physiology, Seed Ecology, Modeling, Seed Health, Germination Characteristics, Seed Dormancy, Seed Ageing, Seed Storage, Seed PrimingVote
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