Volume 10, Issue 2 ((Autumn & Winter) 2024)
Iranian J. Seed Res. 2024, 10(2): 137-150 |
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Majdi M, Tavakkol Afshari R, Khazaee H R, Mirshamsi Kakhki A. (2024). The effects of heat stress on germination and growth of tomato (Solanum lycopersicum) pollen grains under laboratory conditions. Iranian J. Seed Res.. 10(2), : 9 doi:10.61186/yujs.10.2.137
URL: http://yujs.yu.ac.ir/jisr/article-1-600-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-600-en.html
Ferdowsi Univ. of Mashhad , tavakolafshari@um.ac.ir
Abstract: (779 Views)
Extended abstract
Introduction: The effects of temperature increases on the growth of tomato fields are among the obvious results of global warming and are considered an important issue that should be investigated. To maintain and develop the cultivation systems of this crop, a proper understanding of the heat tolerance mechanisms and physiological responses in tomatoes should be achieved. The primary objective of this research is to discover the impact of heat stress on the germination and growth of pollen grains in research tomato germplasms. The researchers' knowledge about the response of different tomato cultivars to abiotic stresses is limited and only the effects of enzymes involved in the response process, heat shock proteins and some hormones have been investigated. The process of detecting heat stress-sensitive stages and their enhancement is facilitated by having a correct understanding of physiological processes.
Materials and methods: The seeds of heat-resistant (LA2661 and LA2662) and -sensitive (LA3911) research cultivars of tomato were used to evaluate the effects of increasing day and night temperatures. The obtained seedlings were grown under optimal temperature conditions (24°C day/18°C night), and after observing the first flower primordium, were incubated in growth chambers to apply daytime heat stress treatments, including temperatures of 28°C, 32°C and 36°C day/18°C night and night stress treatments including temperatures of 28°C, 32°C, and 36°C at night/ 24°C day for 7 days. Pollen grains were then evaluated for their survival, germination, and growth.
Results: The findings of the daytime heat stress tests show that the percentage of survival and germination of pollen grains and growth of pollen tubes of cultivars LA2661, LA2662 and LA3911 decreased as daytime temperature rose from 24°C to 36°C. This reduction is more noticeable for the sensitive cultivar LA3911. Degraded pollen grains increased in the LA3911 cultivar due to heat stress. The survival percentage of pollen grains in all three studied cultivars decreased due to the application of heat stress at night. The resistant cultivars LA2661 and LA2662 had a higher germination percentage compared to the sensitive cultivar LA3911. Pollen grains germination decreased by 50% as a result of increasing the night temperature from 18°C to 36°C. Pollen tube length was reduced in both cultivars and night treatments.
Conclusion: The effects of heat stress in the early stages of flowering when flowers are visible are high, and reproductive stages are very sensitive to high temperatures and affect fertility and processes after insemination, and finally, they lead to yield loss. The daytime temperature increase relative to the natural temperature range (22°C to 24°C) during growth severely impacts the number of pollen grains released from tomato flowers. The number of non-living pollen grains is higher at 36°C day and 32°C and 36°C night temperatures compared to optimal temperature conditions. It appears that the increase in nighttime temperature results in more severe consequences than the increase in daytime temperature.
Highlights:
Introduction: The effects of temperature increases on the growth of tomato fields are among the obvious results of global warming and are considered an important issue that should be investigated. To maintain and develop the cultivation systems of this crop, a proper understanding of the heat tolerance mechanisms and physiological responses in tomatoes should be achieved. The primary objective of this research is to discover the impact of heat stress on the germination and growth of pollen grains in research tomato germplasms. The researchers' knowledge about the response of different tomato cultivars to abiotic stresses is limited and only the effects of enzymes involved in the response process, heat shock proteins and some hormones have been investigated. The process of detecting heat stress-sensitive stages and their enhancement is facilitated by having a correct understanding of physiological processes.
Materials and methods: The seeds of heat-resistant (LA2661 and LA2662) and -sensitive (LA3911) research cultivars of tomato were used to evaluate the effects of increasing day and night temperatures. The obtained seedlings were grown under optimal temperature conditions (24°C day/18°C night), and after observing the first flower primordium, were incubated in growth chambers to apply daytime heat stress treatments, including temperatures of 28°C, 32°C and 36°C day/18°C night and night stress treatments including temperatures of 28°C, 32°C, and 36°C at night/ 24°C day for 7 days. Pollen grains were then evaluated for their survival, germination, and growth.
Results: The findings of the daytime heat stress tests show that the percentage of survival and germination of pollen grains and growth of pollen tubes of cultivars LA2661, LA2662 and LA3911 decreased as daytime temperature rose from 24°C to 36°C. This reduction is more noticeable for the sensitive cultivar LA3911. Degraded pollen grains increased in the LA3911 cultivar due to heat stress. The survival percentage of pollen grains in all three studied cultivars decreased due to the application of heat stress at night. The resistant cultivars LA2661 and LA2662 had a higher germination percentage compared to the sensitive cultivar LA3911. Pollen grains germination decreased by 50% as a result of increasing the night temperature from 18°C to 36°C. Pollen tube length was reduced in both cultivars and night treatments.
Conclusion: The effects of heat stress in the early stages of flowering when flowers are visible are high, and reproductive stages are very sensitive to high temperatures and affect fertility and processes after insemination, and finally, they lead to yield loss. The daytime temperature increase relative to the natural temperature range (22°C to 24°C) during growth severely impacts the number of pollen grains released from tomato flowers. The number of non-living pollen grains is higher at 36°C day and 32°C and 36°C night temperatures compared to optimal temperature conditions. It appears that the increase in nighttime temperature results in more severe consequences than the increase in daytime temperature.
Highlights:
- Night heat stress was assessed as a factor that influences the germination and survival of tomato pollen grains.
- Image analysis was used to measure the length of the pollen tube.
- The effect of thermal stress on pollination was investigated during a specific period of reproductive growth.
Article number: 9
Type of Study: Research |
Subject:
Seed Physiology
Received: 2024/01/27 | Revised: 2024/06/9 | Accepted: 2024/02/14 | ePublished: 2024/06/9
Received: 2024/01/27 | Revised: 2024/06/9 | Accepted: 2024/02/14 | ePublished: 2024/06/9
References
1. Begcy, K. and Dresselhaus, T. 2018. Epigenetic responses to abiotic stresses during reproductive development in cereals. Plant Reproduction, 31: 343-355. [DOI:10.1007/s00497-018-0343-4] [PMID] []
2. Bhattarai, S., Harvey, J.T., Djidonou, D. and Leskovar, D.I. 2021. Exploring morpho-physiological variation for heat stress tolerance in tomato. Plants, 10: 347. [DOI:10.3390/plants10020347] [PMID] []
3. Bueckert, R.A., Wagenhoffer, G., Hnatowich, C., and Warkentin, T.D. 2015. Effect of heat and precipitation on pea yield and reproductive performance in the field. Canadian Journal of Plant Science, 95: 629-639. [DOI:10.4141/cjps-2014-342]
4. Camejo, D., Rodriguez, P., Morales, M.A., Dell'Amico, J.M., Torrecillas, A. and Alarcon, J.J. 2005. High temperature effects on photosynthetic activity of two tomato cultivars with different heat susceptibility. Journal of Plant Physiology, 162: 281-289. [DOI:10.1016/j.jplph.2004.07.014] [PMID]
5. Cross, R.H., Mckay, S.A.B., Mchughen, A.G. and P.C. Bonham‐Smith. 2003. Heat‐stress effects on reproduction and seed set in Linum usitatissimum L. (flax). Journal of Plant Cell and Environment, 26: 1013-1020. [DOI:10.1046/j.1365-3040.2003.01006.x]
6. Devasirvatham, V., Gaur, P.M., Mallikarjuna, N., Tokachichu, R.N., Trethowan, R.M. and Tan, D.K.Y. 2012. Effect of high temperature on the reproductive development of chickpea genotypes under controlled environments. Functional of Plant Biology, 39(12): 1009-1018. [DOI:10.1071/FP12033] [PMID]
7. Din, J.U., Khan, S.U., Khan, A., Qayyum, A., Abbasi, K.S. and Jenks, M.A. 2015. Evaluation of potential morpho-physiological and biochemical indicators in selecting heat-tolerant tomato (Solanum lycopersicum Mill.) genotypes. Horticulture Environment and Biotechnology, 56(6): 769-776. [DOI:10.1007/s13580-015-0098-x]
8. Ding, X.L., Wang, X., Li, Q., Yu, L.F., Song, Q.J., Gai, J.Y. and Yang, S.P. 2019. Metabolomics studies on cytoplasmic male sterility during flower bud development in soybean. International Journal of Molecular Sciences, 20: 28-69. [DOI:10.3390/ijms20122869] [PMID] []
9. Djanaguiraman, M., Prasad, P.V.V., Boyle, D.L., and Schapaugh, W.T. 2013.Soybean pollen anatomy, viability and pod set under high temperature stress. Journal of Agronomy and Crop Sciences, 199: 171-177. [DOI:10.1111/jac.12005]
10. Driedonks, N., Rieu, I. and Vriezen, W.H. 2016. Breeding for plant heat tolerance at vegetative and reproductive stages. Plant Reproduction, 29: 67-79. [DOI:10.1007/s00497-016-0275-9] [PMID] []
11. Erickson, A.N., and Markhart, A.H. 2002. Flower developmental stage and organ sensitivity of bell pepper (Capsicum annuum L.) to elevated temperature. Journal of Plant Cell and Environment, 25: 123-130. [DOI:10.1046/j.0016-8025.2001.00807.x]
12. Hafidh, S., Potesil, D., Muller, K., Michailidis, C., Herrmannova, A., Fecikova, J., Ischebeck, T., Valasek, LS. and Zdrahal, Z. 2018. Dynamics of the pollen sequestrome defined by subcellular coupled omics. Journal of Plant Physiology, 178: 258-282. [DOI:10.1104/pp.18.00648] [PMID] []
13. Jagadish, K.S.V., Craufurd, P., Shi, W. and Oane, R. 2014. A phenotypic marker for quantifying heat stress impact during microsporogenesis in rice (Oryza sativa L.). Journal of Functional Plant Biology, 41: 48-55. [DOI:10.1071/FP13086] [PMID]
14. Jiang, Y., Lahlali, R., Karunakaran, C., Warkentin, T.D., Davis, AR. and Bueckert, RA. 2019. Pollen, ovules, and pollination in pea: success, failure, and resilience in heat. Journal of Plant, Cell and Environment, 42: 354-372. [DOI:10.1111/pce.13427] [PMID]
15. Jiang, Y., Lahlali, R., Karunakaran, C., Warkentin, T.D., Davis, AR. and Bueckert, RA. 2015. Seed set, pollen morphology and pollen surface composition response to heat stress in field pea. Journal of Plant Cell and Environment, 38: 2387-2397. [DOI:10.1111/pce.12589] [PMID]
16. Lahlali, R., Jiang, Y., Kumar, S., Karunakaran, C., Liu, X., Borondics, F., Hallin, E. and Bueckert R. 2014. ATR-FTIR spectroscopy reveals involvement of lipids and proteins of intact pea pollen grains to heat stress tolerance. Frontiers in Plant Science, 5: 747-762. [DOI:10.3389/fpls.2014.00747] [PMID] []
17. Morrison, M.J., Gutknecht, A., Chan, J. and Miller, S.S. 2016. Characterising canola pollen germination across a temperature gradient. Crop Pasture Science, 67: 317-322. [DOI:10.1071/CP15230]
18. Rajametov, S.N., Yang, E.Y., Jeong, H.B., Cho, M.C., Chae, S.Y. and Paudel, N. 2021. Heat treatment in two tomato cultivars: A study of the effect on physiological and growth recovery. Horticulturae, 7(5): 119-128. [DOI:10.3390/horticulturae7050119]
19. Shivanna, K.R. 2003. Pollen Biology and Biotechnology (1st ed.). CRC Press.
20. Singh, V., Nguyen, C.T., Yang, Z., Chapman, S.C., Oosterom, E.J. and Hammer, G.L. 2016. Genotypic differences in effects of short episodes of high-temperature stress during reproductive development in sorghum. Crop Science, 56(4): 1561-1572. [DOI:10.2135/cropsci2015.09.0545]
21. Sita, K., Sehgal, A., Hanumantha, B., Nair, R.M., Prasad, V., Kumar, S., Gaur, P.M., Farooq, M., Siddique, K.H.M., Varshney, R.K. and Nayyar, H. 2017. Food legumes and rising temperature: Effects, adaptive functional mechanisms specific to reproductive growth stage and strategies to improve heat tolerance. Frontiers in Plant Science, 8: 165-172. [DOI:10.3389/fpls.2017.01658] [PMID] []
22. Song, G., Wang, M., Zeng, B., Zhang, J., Jiang, C., Hu, Q., Geng, G. and Tang, C. 2015. Anther response to high-temperature stress during development and pollen thermotolerance heterosis as revealed by pollen tube growth and in vitro pollen vigor analysis in upland cotton. Planta, 241: 1271-1285. [DOI:10.1007/s00425-015-2259-7] [PMID]
23. Sun, M., Jiang, F., Zhang, C., Shen, M. and Wu, Z. 2016. A new comprehensive evaluation system for thermo-tolerance in tomato at different growth stage. Journal of Agricultural Science and Technology B, 6: 152-168. [DOI:10.17265/2161-6264/2016.03.002]
24. Xu, J., Wolters-Arts, M., Mariani, C., Huber, H. and Rieu, I. 2017. Heat stress affects vegetative and reproductive performance and trait correlations in tomato (Solanum lycopersicum). Euphytica, 8: 213-156. [DOI:10.1007/s10681-017-1949-6]
25. Zandalinas, S.I., Fritschi, F.B. and Mittler, R. 2021. Global warming, climate change, and environmental pollution: Recipe for a multifactorial stress combination disaster. Journal of Trends in Plant Science, 26: 588-599. [DOI:10.1016/j.tplants.2021.02.011] [PMID]
26. Zheng, E.h., De Su, S., Xiao, H. and Tian, H., 2019. Calcium: A critical factor in pollen germination and tube elongation. International Journal of Molecular Science, 20(2): 420-431. [DOI:10.3390/ijms20020420] [PMID] []
27. Zhou, R., Kjaer, K., Rosenqvist, E.,Yu, X., Wu, Z., and Ottosen, C.O. 2017. Physiological response to heat stress during seedling and anthesis stage in tomato genotypes differing in heat tolerance. Journal of Agronomy and Crop Science, 203: 68-80. [DOI:10.1111/jac.12166]
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