Volume 10, Issue 2 ((Autumn & Winter) 2024)
Iranian J. Seed Res. 2024, 10(2): 99-118 |
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Savaedy Z, Bakhshandeh A M, Siadat S A, Lotfi Jalal Abadi A, Moosavi S A. (2024). The effect of hormonal priming with cytokinin on deteriorated Nigella (Nigella sativa) seeds. Iranian J. Seed Res.. 10(2), : 7 doi:10.61186/yujs.10.2.99
URL: http://yujs.yu.ac.ir/jisr/article-1-594-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-594-en.html
Zeynab Savaedy 
, Abdol Mehdi Bakhshandeh , Seyed Ataollah Siadat , Amin Lotfi Jalal Abadi , Seyed Amir Moosavi
, Abdol Mehdi Bakhshandeh , Seyed Ataollah Siadat , Amin Lotfi Jalal Abadi , Seyed Amir Moosavi
Agricultural Science and Natural Resources University of Khuzestan , amehdibakhshandeh@gmail.com
Abstract: (929 Views)
Extended abstract
Introduction: Deterioration reduces the quality of seeds. Oilseeds like Nigella are highly susceptible to seed aging. Seed priming enhances the quality of deteriorated seeds by improving germination indices and increasing the activity of antioxidant enzymes. This research aimed to investigate the extent of damage caused by accelerated aging treatment on the germination characteristics and antioxidant enzyme activity of Nigella seeds and the possibility of mitigating the adverse effects of aging through hormonal priming with cytokinin.
Materials and methods: This research was carried out in the form of a completely random basic design with four replications in the seed technology laboratory of Khuzestan University of Agricultural Sciences and Natural Resources in 2017. The treatments included hormonal priming with cytokinin at five levels (0 (control), 10, 20, 40, and 80 m/l) for two durations (12 and 24 hours), and aging under 100% relative humidity and a temperature of 45 °C at five levels (no aging, 24, 48, 72, and 96 h).
Results: The analysis of variance results indicated that germination indices were only influenced by main and two-way effects at the 5% and 1% probability levels, while the three-way interactions, including aging, hormone concentration, and priming duration, were significant for plant growth and longitudinal and weight indices at the 1% probability level. Furthermore, it was evident that the priming treatment mitigated the negative effects of aging, with the concentration of 10 milligrams per liter of cytokinin for a duration of 12 h having the most significant impact among the hormone concentrations used on the measured traits. The highest germination percentage (88%) and the lowest germination percentage (63.33%) were observed at concentrations of 10 and 80 mg/l, respectively. The use of cytokinin at optimal concentration improved catalase activity and protein levels. The results showed that in the control conditions, the activity of the catalase enzyme was 0.76 units per mg of protein and the amount of protein was 0.51 mg/g, which reached 0.97 units per mg of protein and 0.79 mg/g with seed priming.
Conclusion: Based on the results obtained from this research, aging led to a reduction in germination indices, the activity of antioxidant enzymes, and seed protein content. The best treatment applied was cytokinin hormone priming for aged Nigella seeds at a concentration of 10 mg/l for 12 h. According to the results, the application of cytokinin at its optimal concentration (10 mg/l) improved the catalase enzyme activity and protein content. Therefore, it can be suggested that hormonal priming with cytokinin helps mitigate the adverse effects of aging in Nigella plants.
Highlights:
Introduction: Deterioration reduces the quality of seeds. Oilseeds like Nigella are highly susceptible to seed aging. Seed priming enhances the quality of deteriorated seeds by improving germination indices and increasing the activity of antioxidant enzymes. This research aimed to investigate the extent of damage caused by accelerated aging treatment on the germination characteristics and antioxidant enzyme activity of Nigella seeds and the possibility of mitigating the adverse effects of aging through hormonal priming with cytokinin.
Materials and methods: This research was carried out in the form of a completely random basic design with four replications in the seed technology laboratory of Khuzestan University of Agricultural Sciences and Natural Resources in 2017. The treatments included hormonal priming with cytokinin at five levels (0 (control), 10, 20, 40, and 80 m/l) for two durations (12 and 24 hours), and aging under 100% relative humidity and a temperature of 45 °C at five levels (no aging, 24, 48, 72, and 96 h).
Results: The analysis of variance results indicated that germination indices were only influenced by main and two-way effects at the 5% and 1% probability levels, while the three-way interactions, including aging, hormone concentration, and priming duration, were significant for plant growth and longitudinal and weight indices at the 1% probability level. Furthermore, it was evident that the priming treatment mitigated the negative effects of aging, with the concentration of 10 milligrams per liter of cytokinin for a duration of 12 h having the most significant impact among the hormone concentrations used on the measured traits. The highest germination percentage (88%) and the lowest germination percentage (63.33%) were observed at concentrations of 10 and 80 mg/l, respectively. The use of cytokinin at optimal concentration improved catalase activity and protein levels. The results showed that in the control conditions, the activity of the catalase enzyme was 0.76 units per mg of protein and the amount of protein was 0.51 mg/g, which reached 0.97 units per mg of protein and 0.79 mg/g with seed priming.
Conclusion: Based on the results obtained from this research, aging led to a reduction in germination indices, the activity of antioxidant enzymes, and seed protein content. The best treatment applied was cytokinin hormone priming for aged Nigella seeds at a concentration of 10 mg/l for 12 h. According to the results, the application of cytokinin at its optimal concentration (10 mg/l) improved the catalase enzyme activity and protein content. Therefore, it can be suggested that hormonal priming with cytokinin helps mitigate the adverse effects of aging in Nigella plants.
Highlights:
- The impact of hormonal priming with cytokinin at concentrations of 10, 20, 40, and 80 mg/L on aged Nigella seeds was investigated.
- The use of a concentration of 10 mg/l of cytokinin hormone for 12 hours was introduced as the best treatment.
- Cytokinin was introduced as a significant hormone that enhances the activity of antioxidant enzymes and physiological traits in aged Nigella seeds.
Article number: 7
Type of Study: Research |
Subject:
Seed Physiology
Received: 2023/12/17 | Revised: 2024/06/9 | Accepted: 2024/03/9 | ePublished: 2024/06/9
Received: 2023/12/17 | Revised: 2024/06/9 | Accepted: 2024/03/9 | ePublished: 2024/06/9
References
1. Abdul-Baki, A.A. and Anderson, A.J.D. 1973. Vigor determination in soybean by multiple criteria. Crop Science, 13: 630-633. [DOI:10.2135/cropsci1973.0011183X001300060013x]
2. Aebi, H.E. 1983. Catalase. In: Bergmeyer, H.U., Ed., Methods of Enzymatic Analysis, Verlag Chemie, Weinhem, 273-286.
3. Agrawal, R. 2003. Seed technology. Publication. Co. PVT. LTD. New Delhi. India. 64: 229-236.
4. Al Rowais. N.A. 2002. Herbal medicine in the treatment of diabetes mellitus. Saudi Medical Journal, 23: 27-31.
5. Andoh, H. and Kobata, T. 2002. Effect of seed hardening on the seedling emergence and alpha amylase activity in the grains of wheat and rice sown in dry soil. Japanese Journal of Crop Science, 71: 220-225. [DOI:10.1626/jcs.71.220]
6. Azadi, M.S., Tabatabaei, S.A., Younesi, E., Rostami, M.R. and Mombeini, M. 2013. Hormone priming improves germination characteristics and enzyme activity of sorghum seeds (Sorghum bicolor L.) under accelerated aging. Cercetari Agronomice in Moldova, 3(155): 49-56. [DOI:10.2478/v10298-012-0092-8]
7. Bailly. C. 2004. Active oxygen species and antioxidants in seed biology. Seed Science Research, 14: 93-107. [DOI:10.1079/SSR2004159]
8. Baladi, S., Balouchi, H.R., Moradi, A. and Movahhedi Dehnavi, M. 2016. Effect of different temperatures and moisture during storage on germination indices of oilseed. Journal of Seed Science and Technology, 5(1): 107-122. [In Persian with English Summary].
9. Basra, S.M.A., Ahmad, N., Khan, M.M., Iqbal, N. and Cheema, M.A. 2003. Assessment of cotton seed deterioration during accelerated aging. Seed Science and Technology, 31: 531-540. [DOI:10.15258/sst.2003.31.3.02]
10. Black, M. and Bewley, J.D. 2009. Seed Technology and its Biological Basis. Translated by R, TavakkolAfshari. A, AbbasiSurki. E, Ghasemi. University of Tehran Press. 515 pages. [In Persian].
11. Bobak, S.A., Parvis, N.K. and Ansari, W.M. 2015. An assessment of the effects of seed ageing application of phytohormone and kno on aged corn seeds. African Journal of Agronomy, 3: 235-243. [In Persian with English Summary].
12. Bradford, K.J. 1995. Water relations in seed germination. "Seed Development and Germination" (J. Kigel and G. Galili, Eds.), Marcel dekkerinc. New York. pp, 351-396. [DOI:10.1201/9780203740071-13]
13. Brancalion, P.H.S., Novembre, A.D.L.C., Rodrigues, R.R. and Tay, D. 2008. Priming of Mimosa bimucronata seeds: a tropical tree species from Brazil. Journal of Acta Horticulturae, 82: 163-168. [DOI:10.17660/ActaHortic.2008.782.18]
14. Bray, C.M. 1995. Biochemical processes during the osmopriming of seeds, in: Kigel, Y., Galili, G. (Eds.). Seed Development and Germination, 767-789. [DOI:10.1201/9780203740071-28]
15. Casenave, E.C. and Toselli, M.E. 2007. Hydropriming as a pre-treatment for cotton germination under thermal and water stress conditions. Seed Science and Technology, 35: 88-98. [DOI:10.15258/sst.2007.35.1.08]
16. Eisvand, H.R., Alizadeh, M.A. and Fekri, A. 2010. How Hormonal priming of aged and nonaged seeds of bromegrass affects seedling physiological characters. Journal of New Seeds, 11(1): 52-64. [DOI:10.1080/15228860903584523]
17. Ellis, R.H. and Roberts, E.H. 1981. The quantification of aging and survival in orthodox seeds. Seed Science and Technology, 9: 373-409.
18. Farooq, M., Basra, S.M.A., Warraich, E.A. and Khaliq, A. 2006. Optimization of hydro priming Techniques for rice seed invigoration. Seed Science and Technology, 34: 529-534. [DOI:10.15258/sst.2006.34.2.25]
19. Foti, R., Abureni, K., Tigere, A., Gotosa, J. and Gere, J. 2008. The efficacy of different seed priming osmotica on the establishment of maize (Zea mays L.) caryopses. Journal of Arid Environments, 72: 1127-1130. [DOI:10.1016/j.jaridenv.2007.11.008]
20. Fujikura, Y. and Karssen, C.M. 1995. Molecular studies on osmoprimed seeds of cauliflower: a partial amino acid sequence of a vigour-related protein and osmopriming. Seed Science Research, 5: 177-181. [DOI:10.1017/S0960258500002804]
21. Goel, A., Goel, A.K. and Sheoran, I.S. 2003. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. Journal of Plant Physiology, 160: 1093-1100. [DOI:10.1078/0176-1617-00881] [PMID]
22. Goreja, W.G. 2003. Black Seed: Nature's Miracle Remedy, Amazing Herbs Press, New York, NY.
23. Hemeda, H.M. and Kelin, B.P. 1990. Effects of naturally occurring antioxidants on peroxidase activity of vegetables extracts. Journal of Food Science, 55: 184-192. [DOI:10.1111/j.1365-2621.1990.tb06048.x]
24. Hosseini, A. and Kuchaki, A.R. 2007. Effect of different treatments on germination and germination rate of four Sugar Beet cultivars. Iranian Journal of Agricultural Research, 5(1): 69-76. [In Persian with English Summary].
25. Hosseini, F. 2000. The effect of seed deterioration on germination, establishment and yield of five Rapeseed varieties in Ahvaz climatic conditions. Master Thesis Agriculture and Natural Resources University of Ahvaz, 285 p. [In Persian with English Summary].
26. Hsu, C.C., Chen, C.L., Chen, J.J. and Sung, J.M. 2003. Accelerated aging-enhanced lipid peroxidation in bitter gourd seeds and effects of priming and hot water soaking treatments. Scientia Horticulture, 98: 201-212. [DOI:10.1016/S0304-4238(03)00002-5]
27. Ikic, I., Maricevic. M., Tomasovic. S., Gunjaca, Z.S. and Atovic, H.S. 2012. Arcevic the effect of germination temperature on seed dormancy in Croatian-grown winter wheats. Euphytica, 188: 25-34. [DOI:10.1007/s10681-012-0735-8]
28. ISTA. 2012. International rules for seed testing, edition 2012. Did you mean: International Seed Testing Association Bassersdorf, Switzerland.
29. Jiang, Y. and Huang, B. 2001. Drought and heat stress injury to two cool-season turf grasses in relation to antioxidant metabolism and lipid peroxidation. Crop Science, 41: 436-442. [DOI:10.2135/cropsci2001.412436x]
30. Jisha, K.C., Vijayakumari, K. and Puthur, J.T. 2013. Seed priming for abiotic stress tolerance: an overview. Acta Physiologiae Plantarum, 35: 1381-1396. [DOI:10.1007/s11738-012-1186-5]
31. Jyoti, C. and Malik, P. 2013. Seed deterioration: a review. International Journal of Life Sciences and Biotechnology Pharma Research, 2(3): 374- 385.
32. Kanto, V., Jutamanec, K., Osotspar, Y., Chaiarree, W. and Jattupornpang, S. 2015. Promotive effect of priming with 5-Amonolevulinic acid on seed germination capacity, seedling growth and antioxidant enzyme activity in rice subjected to accelerated aging treatment. Plant Production Science, 18: 443-454. [DOI:10.1626/pps.18.443]
33. Khan, M.A., Gurchani, M.A., Hussain, M., Freed, S. and Mahmood, K. 2011. Wheat seed enhancement by vitamin and hormonal priming. Pakistan Journal of Botany, 43(3): 1495-1499.
34. Kibinza, S., Bazin, J., Bailly, C., Farrant, J. M., Corbineau, O. and El-Maarouf-Bouteau, H. 2011. Catalase is a key enzyme in seed recovery from ageing during priming. Plant Science, 181: 309-315. [DOI:10.1016/j.plantsci.2011.06.003] [PMID]
35. Kirshnan, P., Nagarajan, S., Dadlani, M. and Moharir, A.V. 2003. Characterization of wheat (Triticum aestivum) and soybean (Glycine max) seeds under accelerated ageing condition by proton nuclear magnetic spectroscopy. Seed Science and Technology, 31: 541-550. [DOI:10.15258/sst.2003.31.3.03]
36. Macdonald, C.M., Floyd, C.D. and Waniska, R.D. 2004. Effect of accelerated aging on maize and sorghum. Journal of Cereal Science, 39: 351-361. [DOI:10.1016/j.jcs.2004.01.001]
37. Marshal, A.H., and Lewis, D.N. 2004. Influence of seed storage conditions on seedling emergence, seedling growth and dry matter production of temperature forage grasses. Seed Science and Technology, 32: 493-501. [DOI:10.15258/sst.2004.32.2.19]
38. McDonald, M.B. 1999. Seed deterioration: physiology, repair and assessment. Seed Science and Technology, 27: 177-237.
39. Morris, D.A. and Arthur, E.D. 1984. Invertase and auxin-induced elongation in intermodal segments Phaseolus vulgaris. Phytochemistry, 23(10): 2163-2167. [DOI:10.1016/S0031-9422(00)80512-9]
40. Muller, B. and Sheen, J. 2007. Advances in cytokinin signaling. Science, 318(68): 68-69. [DOI:10.1126/science.1145461] [PMID]
41. Murthy, U.M.N., Kumar, P.D. and Sun, W.Q. 2003. Mechanisms of seed aging under different storable conditions for Vigna radiata L. wilczek. Lipid peroxidation, sugar hydrolysis, Maillavdn reactions and their relationship to state transition. Journal of Experimental Botany, 384: 1057-1067. [DOI:10.1093/jxb/erg092] [PMID]
42. Nascimento, W.M. and Aragao, F.A.S. 2004. Muskmelon seed priming in relation to seed vigor. Scientia Agricola, 61(1): 114-117. [DOI:10.1590/S0103-90162004000100019]
43. Noctor, G. and Foyer, C. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 49: 249-279. [DOI:10.1146/annurev.arplant.49.1.249] [PMID]
44. Parmoon, G.h., Ebadi, A., Jahanbakhsh Godahkahriz, S. and Davari, M. 2014. Effect of seed priming by salicylic acid on the physiological and biochemical traits of aging milk thistle (Silybum marianum) seeds. Electrical Journal of Crop Production, 7(4): 223-234. [In Persian with English Summary].
45. Rashidi, S., Abbas Dokht, H. and Hholami, A. 2017. Effect of gibberellin and cytokinin on improvement of germination traits and degraded seeds of corn cultivars. Journal of Medicinal Plants Physiology, 9(34) 79-96. [In Persian with English Summary].
46. Rehman, H., Iqbal, H., Basra, S. M.A., Afzal, I., Farooq, M., Wakeel, A. and Ning, W. 2015. Seed priming improves early seedling vigor, growth and productivity of spring maize. Journal Integrative Agriculture, 14(9): 1745-1754. [DOI:10.1016/S2095-3119(14)61000-5]
47. Rouhi, H.R., Aboutalebian, M.A., Moosavi, S.A., Karimi, F.A.O., Karimi, F., Saman, M. and Samadi, M. 2012. Change in several antioxidant enzymes activity of Berseem clover (Trifolium alexandrinum L.) by priming. International Journal of Agricultural Science, 2(3): 237-243. [In Persian with English Summary].
48. Savaedi, Z., Parmoon, G., Moosavi, S.A., and Bakhshande A.B. 2019. The role of light and gibberellic acid on cardinal temperatures and thermal time required for germination of Charnushka (Nigella sativa) seed. Industrial Crops and Products, 132: 140-149. [DOI:10.1016/j.indcrop.2019.02.025]
49. Siadat, S.A., Moosavi, S.A. and Sharifzadeh, M. 2015. Alleviate seed ageing effects in Silybum marianum by application of hormone seed priming. Notulae Scientia Biologicae, 7(3): 316-321.
https://doi.org/10.15835/nsb.7.3.9528 [DOI:10.15835/nsb739528]
50. Tavakol Afshari, R., Rashidi, S. and Alizadeh, H. 2009. Effect strong on seed germination and activities of catalase and peroxidase enzymes in the initial stages of germination of two cultivars of rapeseed (Brassica napus L.). Iranian Journal of Crop Sciences, 2: 125-133. [In Persian with English Summary].
51. Varier, A., Vari, A.K. and Dadlani, M. 2010. The subcellular basis of seed priming. Current Science, 99(4): 450-456.
52. Verma, S. K., Bjpai, G.C., Tewari, S.K. and Singh, J. 2005. Seedling index and yield as influenced by seed size in pigeon pea. Legume Research, 28(2): 143-145.
53. Xia, F., Wang, X., Li, M. and Mao, P. 2015. Mitochondrial structural and antioxidant system responses to aging in oat (Avena sativa L.) seeds with different moisture contents. Plant Physiology and Biochemistry, 94: 122-129. [DOI:10.1016/j.plaphy.2015.06.002] [PMID]
54. Zhang, M., Wang, Z., Yuan, L., Yin, C., Cheng, J., Wang, L., Huang, J. and Zhang, H. 2014. Osmopriming improves tomato seed vigor under aging and salinity stress. African Journal of Biotechnology, 11(23): 6305-6311.
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