Volume 10, Issue 2 ((Autumn & Winter) 2024)
Iranian J. Seed Res. 2024, 10(2): 21-36 |
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saadat H, Sedghi M, Seyed Sharifi R, Farzaneh S. (2024). The effect of chitosan priming on germination indices and biochemical characteristics of French bean seeds (Phaseolus vulgaris cv. Sadri) under salinity stress. Iranian J. Seed Res.. 10(2), : 2 doi:10.61186/yujs.10.2.21
URL: http://yujs.yu.ac.ir/jisr/article-1-573-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-573-en.html
Department of Agronomy, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabili, Iran. , t.saadat2020@gmail.com
Abstract: (1092 Views)
Extended Abstract
Introduction: At present, the drastic increase in population has created an additional burden on the world's agricultural resources. As a result, meeting global food demand and increasing farmers' incomes has become a challenging task. Salinity is one of the abiotic stresses that strongly affect the germination, growth, and yield of crops. Seed priming is a simple technology that hydrates seeds to the point where the metabolic activity for germination is initiated without radicle emergence. In fact, the seeds are physiologically and biochemically prepared to germinate before being placed in their bed and exposed to environmental conditions. This research was conducted to investigate the effect of seed priming with chitosan on germination indices and biochemical traits of beans under salt stress.
Materials and Methods: This experiment was conducted at the University of Mohaghegh Ardabili in 2021 as a factorial in the form of a completely randomized design with four repetitions. The treatments included four levels of salinity (0, 50, 100, and 150 mM) and four levels of chitosan (0, 0.25, 0.50, and 0.75% w/v), all of which were dissolved in 1% acetic acid.
Results: The results showed that salinity stress decreased germination percentage (GP), peak value (PV), and germination value (GV). However, seed priming with different levels of chitosan, especially 0.75% chitosan, improved GP, PV, and GV by 7, 21, and 17%, respectively, compared to the control. Proline content and polyphenol oxidase enzyme activity went up with increasing salinity. However, the application of chitosan 0.75% increased these traits by 34% and 43%, respectively, compared to the control (priming with distilled water). Electrolyte leakage in priming with 0.75% chitosan decreased by 31% compared to the control, which indicates the maintenance of the cell membrane stability. The content of soluble sugars in the treatment with 0.75% chitosan and 150 mM salinity showed an increase of about 78% compared to the control. The highest correlation among traits was observed between peak value and germination coefficient (r2=0.99) and between proline and polyphenol oxidase enzyme (r2=0.92).
Conclusions: The results of this study show that among the different treatments, pre-treatment of seeds with 75 % Chitosan may be considered an effective way to improve germination indices and biochemical characteristics of beans and it can be used as a treatment to deal with salinity conditions in bean seedlings and improve their seedling growth.
Highlights:
Introduction: At present, the drastic increase in population has created an additional burden on the world's agricultural resources. As a result, meeting global food demand and increasing farmers' incomes has become a challenging task. Salinity is one of the abiotic stresses that strongly affect the germination, growth, and yield of crops. Seed priming is a simple technology that hydrates seeds to the point where the metabolic activity for germination is initiated without radicle emergence. In fact, the seeds are physiologically and biochemically prepared to germinate before being placed in their bed and exposed to environmental conditions. This research was conducted to investigate the effect of seed priming with chitosan on germination indices and biochemical traits of beans under salt stress.
Materials and Methods: This experiment was conducted at the University of Mohaghegh Ardabili in 2021 as a factorial in the form of a completely randomized design with four repetitions. The treatments included four levels of salinity (0, 50, 100, and 150 mM) and four levels of chitosan (0, 0.25, 0.50, and 0.75% w/v), all of which were dissolved in 1% acetic acid.
Results: The results showed that salinity stress decreased germination percentage (GP), peak value (PV), and germination value (GV). However, seed priming with different levels of chitosan, especially 0.75% chitosan, improved GP, PV, and GV by 7, 21, and 17%, respectively, compared to the control. Proline content and polyphenol oxidase enzyme activity went up with increasing salinity. However, the application of chitosan 0.75% increased these traits by 34% and 43%, respectively, compared to the control (priming with distilled water). Electrolyte leakage in priming with 0.75% chitosan decreased by 31% compared to the control, which indicates the maintenance of the cell membrane stability. The content of soluble sugars in the treatment with 0.75% chitosan and 150 mM salinity showed an increase of about 78% compared to the control. The highest correlation among traits was observed between peak value and germination coefficient (r2=0.99) and between proline and polyphenol oxidase enzyme (r2=0.92).
Conclusions: The results of this study show that among the different treatments, pre-treatment of seeds with 75 % Chitosan may be considered an effective way to improve germination indices and biochemical characteristics of beans and it can be used as a treatment to deal with salinity conditions in bean seedlings and improve their seedling growth.
Highlights:
- Seed priming using chitosan improved germination indices of common bean seeds under salt stress.
- Priming with chitosan increased proline and polyphenol oxidase enzyme.
- A chitosan concentration of 0.75 V/W showed a better effect on germination indices and biochemical characteristics.
- Priming with chitosan can be a suitable method to mitigate the negative effects of salinity, increase germination indices, and improve the biochemical characteristics of beans.
Article number: 2
Type of Study: Research |
Subject:
Seed Physiology
Received: 2023/01/19 | Revised: 2024/07/13 | Accepted: 2023/04/20 | ePublished: 2024/06/9
Received: 2023/01/19 | Revised: 2024/07/13 | Accepted: 2023/04/20 | ePublished: 2024/06/9
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