Volume 9, Issue 2 ((Autumn & Winter) 2023)                   Iranian J. Seed Res. 2023, 9(2): 77-98 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohammadi M, Tavakol Afshari R, Nabati J, Oskoueian E. (2023). Evaluating the possibility of increasing freezing tolerance in chickpeas (Cicer arietinum) by seed priming. Iranian J. Seed Res.. 9(2), 77-98.
URL: http://yujs.yu.ac.ir/jisr/article-1-542-en.html
Ferdowsi University of Mashhad , tavakolafshari@um.ac.ir
Abstract:   (779 Views)
Extended Abstract
Introduction: One of the major reasons behind the unstable yield of chickpea, is the simultaneity of the reproductive stage with drought and late-season heat. Autumn sowing of chickpea is among the suitable approaches to improve chickpea yield. On the other hand, freezing stress is a limiting factor in the autumn sowing of chickpea. Recently, seed priming has been developed as an essential method to induce plant tolerance to environmental stress. The priming will result in a rapid response of the plant to stress. Freezing, as an environmental stress, limits the growth and development of many plants in different parts of the world. Studies show that in addition to acclimation, short-term biotic and abiotic stresses as pretreatment could also increase the plant's tolerance to cold stress. This process alters the freezing response positively.
Material and Methods: This experiment was conducted as a factorial in a completely randomized design with three replicates at the greenhouse of the Research Center for Plant Sciences of Ferdowsi University, Mashhad Iran, in 2018. The experimental factors consisted of various temperatures (0, -12, -15, and -17 °C), seed priming at 10 levels (control (without priming), hydropriming, priming with sodium chloride, salicylic acid, sodium nitroprusside, phosphate solubilizing bacteria and potassium solubilizing bacteria, amino acids, potassium nitrate, and zinc sulfate) and different chickpea genotypes (MCC505, ILC8617, MCC495, and Saral cultivar). In this experiment, the measured parameters included survival percentage, electrolyte leakage percentage, and lethal temperature resulting in 50% mortality according to the electrolyte leakage and survival percentage.
Results: The results showed that the application of hydropriming, priming with sodium nitroprusside and zinc sulfate had favorable effects on the survival rate and electrolyte leakage. Among these, priming with sodium nitroprusside increased the survival percentage compared to the control (23%) at the -15 and -17 °C in the Saral cultivar, at -15 °C in the ILC8617 genotype, and at -12 and -15 °C in the MCC495 genotype treatment to 68, 58, 85 and 55 percent, respectively. In addition, this treatment reduced the electrolyte leakage by 13% at -15 °C in the ILC8617 genotype compared to the control treatment. Further, the mentioned treatment resulted in a 40% reduction in lethal temperature resulting in 50% mortality according to the survival percentage. In the MCC495 genotype compared to the control treatment.
Conclusion: Overall, the cold stress in the chickpea plants resulted in an increase in electrolyte leakage and a decrease in the survival percentage. Application of sodium nitroprusside priming by improving cold stress tolerance resulted in a reduction of lethal temperature resulting in 50% mortality based on electrolyte leakage and survival percentage results. Additionally, the applied priming in improving the cold stress tolerance mainly improved the survival percentage compared to the improvement in the electrolyte leakage.

  1. The effect of different primings on the freezing tolerance of chickpeas was investigated and determined.
  2. The freezing tolerance threshold of chickpea seedlings was determined at the laboratory under different primings.
  3. The respondents of genotypes to priming and the behavior of genotypes towards each other were investigated.
Article number: 6
Full-Text [PDF 815 kb]   (117 Downloads)    
Type of Study: Research | Subject: General
Received: 2021/09/23 | Accepted: 2022/04/12

1. Amooaghaie, R. and Nikzad, K. 2013. The role of nitric oxide in priming-induced low-temperature tolerance in two genotypes of tomato. Seed Science Research, 23(2): 123-131. [DOI:10.1017/S0960258513000068]
2. Anderson, J., Kenna, M. and Taliaferro, C. 1988. Cold hardiness of Midiron and Tifgreen bermudagrass. HortScience, 23(4): 748-750. [DOI:10.21273/HORTSCI.23.4.748]
3. Arif, M., Waqas, M., Nawab, K. and Shahid, M. 2007. Effect of seed priming in Zn solutions on chickpea and wheat. African Crop Science Society Conference, 8: 237-240.
4. Bahmani, M., Maali-Amiri, R., Javan-Nikkhah, M., Atghia, O. and Rasolnia, A. 2020. Enhanced tolerance to ascochyta blight in chickpea plants via low temperature acclimation. Russian Journal of Plant Physiology, 67(4): 758-766. [DOI:10.1134/S1021443720040020]
5. Beihaghi, M., Bagheri, A., Bahrami, A. R., Shahriari, F. and Nezami, A. 2010. The possible role of phosphoenolpyruvate carboxykinase (PEPCK) in protein content of chickpea seeds (Cicer arietinum L.). Iranian Journal of Pulses Research, 1(1): 57-64. [In Persian with English Summary].
6. Bibi, A., Majid, S., Ulfat, A., Khatoon, S., Munir, A. and Javed, G. J. J. O. A. 2017. Effect of nitric oxide seed priming on chilling induced water related physiological attributes in germinating wheat. Journal of Animal Plant Sciences, 1: 186-191.
7. Čanak, P., Mirosavljević, M., Ćirić, M., Vujošević, B., Kešelj, J., Stanisavljević, D. and Mitrović, B. 2016. Seed priming as a method for improving maize seed germination parameters at low temperatures. Ratarstvo i Povrtarstvo/Field and Vegetable Crops Research, 53(3): 106-110. [DOI:10.5937/ratpov53-10825]
8. Chaturvedi, S., Jha, S., Singh, N., Gaur, P. and Varshney, R. 2018. Technological and policy intervention for increasing chickpea production in India. Pulse India, 8(1): 7-12.
9. Chen, K. and Arora, R. 2011. Dynamics of the antioxidant system during seed osmopriming, post-priming germination, and seedling establishment in spinach (Spinacia oleracea). Plant Science, 180(2): 212-220. [DOI:10.1016/j.plantsci.2010.08.007] [PMID]
10. Crosatti, C., Pagani, D., Cattivelli, L., Stanca, A. and Rizza, F. 2008. Effects of growth stage and hardening conditions on the association between frost resistance and the expression of the cold-induced protein COR14b in barley. Environmental Experimental Botany, 62(2): 93-100. [DOI:10.1016/j.envexpbot.2007.07.008]
11. Cvetkovic, J., Müller, K. and Baier, M. 2017. The effect of cold priming on the fitness of Arabidopsis thaliana accessions under natural and controlled conditions. Scientific Reports, 7(1): 1-20. [DOI:10.1038/srep44055] [PMID] [PMCID]
12. Devasirvatham, V. and Tan, D.K. 2018. Impact of high temperature and drought stresses on chickpea production. Agronomy, 8(8): 145. [DOI:10.3390/agronomy8080145]
13. Dixit, G., Srivastava, A. and Singh, N. 2019. Marching towards self-sufficiency in chickpea. Current Science, 116(2): 239-242. [DOI:10.18520/cs/v116/i2/239-242]
14. Ebadi, A. and Gollojeh, S. K. 2009. Effects of seed priming on growth and yield of chickpea under saline soil. Recent Research in Science and Technology, 1(6): 282-286.
15. Elias, S.G. and Copeland, L.O. 2001. Physiological and harvest maturity of canola in relation to seed quality. Agronomy Journal, 93(5): 1054-1058. [DOI:10.2134/agronj2001.9351054x]
16. Farahbakhsh, H. 2012. Germination and seedling growth in unprimed and primed seeds of fennel as affected by reduced water potential induced by NaCl. International Research Journal of Applied and Basic Sciences, 3(4): 737-744.
17. Farooq, M., Hussain, M., Nawaz, A., Lee, D. J., Alghamdi, S. S. and Siddique, K. H. 2017. Seed priming improves chilling tolerance in chickpea by modulating germination metabolism, trehalose accumulation and carbon assimilation. Plant Physiology Biochemistry, 111: 274-283. [DOI:10.1016/j.plaphy.2016.12.012] [PMID]
18. Garg, R., Bhattacharjee, A. and Jain, M. 2015. Genome-scale transcriptomic insights into molecular aspects of abiotic stress responses in chickpea. Plant Molecular Biology Reporter, 33(3): 388-400. [DOI:10.1007/s11105-014-0753-x]
19. Gehring, M. and Satyaki, P. 2017. Endosperm and imprinting, inextricably linked. Plant Physiology Biochemistry, 173(1): 143-154. [DOI:10.1104/pp.16.01353] [PMID] [PMCID]
20. Gupta, R. and Deswal, R. 2014. Antifreeze proteins enable plants to survive in freezing conditions. Journal of Biosciences, 39(5): 931-944. [DOI:10.1007/s12038-014-9468-2] [PMID]
21. Heidarvand, L. and Amiri, R. M. 2010. What happens in plant molecular responses to cold stress? Acta Physiologiae Plantarum, 32(3): 419-431. [DOI:10.1007/s11738-009-0451-8]
22. Hiremath, U., Gowda, B., Lokesh, G. and Ganiger, B. 2021. Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.). Journal of Applied Natural Science, 13(2): 735-743. [DOI:10.31018/jans.v13i2.2507]
23. Kadiyala, M., Kumara Charyulu, D., Nedumaran, S.D Shyam, M., Gumma, M. and Bantilan, M. 2016. Agronomic management options for sustaining chickpea yield under climate change scenario. Journal of Agrometeorology, 18(01): 41-47. [DOI:10.54386/jam.v18i1.897]
24. Kazemi-Shahandashti, SS., Maali-Amiri, R., Zeinali, H., Khazaei, M., Talei, A. and Ramezanpour, S.S. 2014. Effect of short-term cold stress on oxidative damage and transcript accumulation of defense-related genes in chickpea seedlings. Journal of Plant Physiology, 171(13): 1106-1116. [DOI:10.1016/j.jplph.2014.03.020] [PMID]
25. Khodabakhsh, F., Amooaghaie, R., Mostajeran, A. and Emtiazi, G. 2011. Effect of hydro and osmopriming in Two commercial chickpea (Cicer arietinum L.) cultivars on germination, growth parameters and nodules number in salt stress condition. Iranian Journal of Plant Biology, 2(4): 71-86. [In Persian with English Summary].
26. Murray, G., Eser, D., Gusta, L. and Eteve, G. 1988. Winterhardiness in pea, lentil, faba bean and chickpea. In World Crops: Cool season food legumes (pp. 831-843): Springer. [DOI:10.1007/978-94-009-2764-3_66]
27. Nezami, A. and Naghedi Nia, N. 2011. Effect of freezing stress on electrolyte leakage in six varieties of safflower. Iranian Journal of Field Crops Research, 8(6): 891-896. [In Persian with English Summary].
28. Okeyo, D.O., Fry, J.D., Bremer, D., Rajashekar, C.B., Kennelly, M., Chandra, A., Genovesi, D.A. and Engelke, M. C. 2011. Freezing tolerance and seasonal color of experimental zoysiagrasses. Crop Science, 51(6): 2858-2863. [DOI:10.2135/cropsci2011.01.0049]
29. Rhaman, M.S., Rauf, F., Tania, S.S. and Khatun, M. 2020. Seed priming methods: application in field crops and future perspectives. Asian Journal of Research in Crop Science, 5(2): 8-19. [DOI:10.9734/ajrcs/2020/v5i230091]
30. Sabaghpour, SH,. Sadeghzadeh- Ahari, D., Mahmoodi, AA., Kanouni, H., Farayedi, Y., Sabaghpour, S., Sadeghzadeh- Ahari, D., Shahab, M., Kamel, M., Saeid, A., Mahmoodi, A., Pezeshkpour, P., Norollahi, K., Hasanpour- Hosni, M., Mahdie, M., Bahrami Kamangir, S., Mahmoodi, F., Nemati-Fard, M., Ghasemi, M. 2013. Saral, new chickpea variety to expand autumn sowing in highland cold areas of Iran. Research Achievements for Field and Horticulture Crops, 2(4): 265-276.
31. Saeed, A., Darvishzadeh, R., Hovsepyan, H. and Asatryan, A. 2010. Tolerance to freezing stress in cicer accessions under controlled and field conditions. African Journal of Biotechnology, 9(18): 2618-2626.
32. Singh, K., Malhotra, R. and Saxena, M. 1995. Additional sources of tolerance to cold in cultivated and wild Cicer species. Crop Science, 35(5): 1491-1497. [DOI:10.2135/cropsci1995.0011183X003500050037x]
33. Szalai, G., Pál, M., Árendás, T. and Janda, T. 2016. Priming seed with salicylic acid increases grain yield and modifies polyamine levels in maize. Cereal Research Communications, 44(4): 537-548. [DOI:10.1556/0806.44.2016.038]
34. Tania, S. S., Rhaman, M. S. and Hossain, M. M. 2020. Hydro-priming and halo-priming improve seed germination, yield and yield contributing characters of okra (Abelmoschus esculentus L.). Tropical Plant Research, 7(1): 86-93. [DOI:10.22271/tpr.2020.v7.i1.012]
35. Varshney, R. K., Song, C., Saxena, R. K., Azam, S., Yu, S., Sharpe, A. G., Cannon, S., Baek, J., Rosen, B. D. and Tar'an, B. 2013. Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement. Nature Biotechnology, 31(3): 240-246. [DOI:10.1038/nbt.2491] [PMID]
36. Wang, Y., Tsukamoto, T., Noble, J. A., Liu, X., Mosher, R. A. and Palanivelu, R. 2017. Arabidopsis LORELEI, a maternally expressed imprinted gene, promotes early seed development. Plant Physiology, 175(2): 758-773. [DOI:10.1104/pp.17.00427] [PMID] [PMCID]
37. Xin, Z. and Browse, J. 2000. Cold comfort farm: the acclimation of plants to freezing temperatures. Plant, Cell Environmental Experimental Botany, 23(9): 893-902. [DOI:10.1046/j.1365-3040.2000.00611.x]
38. Yadav, P., Maya, K. and Zakwan, A. 2011. Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Research Journal of Seed Science, 4(3): 125-136. [DOI:10.3923/rjss.2011.125.136]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb

This work is licensed under a Creative Commons Attribution 4.0 International License.