Volume 9, Issue 1 ((Spring and Summer) 2022)
Iranian J. Seed Res. 2022, 9(1): 163-176 |
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Labbafi M, Khalaj H, Delfani M, Qavami N. (2022). Evaluation of different hormonal and temperature treatments on dormancy breaking of Lavender (Lavandula angustifolia) seed. Iranian J. Seed Res.. 9(1), : 10 doi:10.52547/yujs.9.1.163
URL: http://yujs.yu.ac.ir/jisr/article-1-472-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-472-en.html
Medicinal Plants Research Center, Institute of Medical Plants, ACECR, Karaj, Iran , labbafi@imp.ac.ir
Abstract: (1671 Views)
Extended Abstract
Introduction: Lavandula angustifolia L. is one of the most important plants belonging to Lamiaceae which has abundant use in traditional and pharmacological medicine. Lavender seed dormancy is one of the problems in producing seedlings which increases seed consumption. In this regard, an experiment was carried out to investigate different treatments for seed dormancy elimination of Lavender and measuring germination and growth indices.
Materials and Methods: An experiment was conducted in a completely randomized design with three replications at the Ecophysiology Laboratory of Karaj Institute of Medicinal Plants in 2018. The experiment consisted of 14 treatments on seed dormancy elimination. The treatments included the control, hormonal treatments (Gibberellic acid with 250, 500, 750 and 1000 mg/l concentrations) and Prechilling treatments (7, 14 and 21 days of refrigeration). The integrated treatments were 7 days refrigeration + 500 mg/l gibberellic acid, 14 days refrigeration + 500 mg/l gibberellic acid, 7 days refrigeration + 1000 mg/l gibberellic acid, 14 days refrigeration + 1000 mg/l gibberellic acid, 30 minutes at 40 ° C water + 14 days refrigeration, and 30 minutes at water 40 degrees +14 days refrigeration + 500 mg/l gibberellic acid.
Results: The results showed that the highest germination percentage (70.67%), germination rate (24.26%) and germination index (246) were achieved in 750 mg/l Gibberellic acid, and the highest epicotyl length (5.09 cm), seedling length (6.23 cm) and seedling fresh weight (0.57 gr) were produced with 500 mg/l Gibberellic acid. Also, the lowest mean germination time (MGT) (4.12s day) was obtained in 1000 mg/l Gibberellic acid treatment. Also, 7 days of refrigeration + gibberellic acid at 500 mg/l treatment had no significant difference with the other concentrations of gibberellin treatments. Therefore, low gibberellin concentration with 7 days of prechilling had the same effect as higher gibberellic acid concentrations. In addition, 14 days of refrigeration + 1000 mg/l gibberellic acid treatment and treatment of 30 min in water at 40 ° C +14 days prechilling + 500 mg/l gibberellic acid produced the lowest shoot (0.78, 0.82 cm), seedling height (0.99, 1.04 cm) and fresh weight (0.013, 0.01 gr) that showed the negative effects of increased prechilling time and water at 40 ° C,
Conclusion: It was finally found that the lavender seed dormancy type is physiologically non-deep and moderate and Gibberellic acid is a suitable substitute for prechilling. The best treatment was gibberellic acid with a concentration of 750 mg/l to break the dormancy of lavender seeds.
Highlights:
Introduction: Lavandula angustifolia L. is one of the most important plants belonging to Lamiaceae which has abundant use in traditional and pharmacological medicine. Lavender seed dormancy is one of the problems in producing seedlings which increases seed consumption. In this regard, an experiment was carried out to investigate different treatments for seed dormancy elimination of Lavender and measuring germination and growth indices.
Materials and Methods: An experiment was conducted in a completely randomized design with three replications at the Ecophysiology Laboratory of Karaj Institute of Medicinal Plants in 2018. The experiment consisted of 14 treatments on seed dormancy elimination. The treatments included the control, hormonal treatments (Gibberellic acid with 250, 500, 750 and 1000 mg/l concentrations) and Prechilling treatments (7, 14 and 21 days of refrigeration). The integrated treatments were 7 days refrigeration + 500 mg/l gibberellic acid, 14 days refrigeration + 500 mg/l gibberellic acid, 7 days refrigeration + 1000 mg/l gibberellic acid, 14 days refrigeration + 1000 mg/l gibberellic acid, 30 minutes at 40 ° C water + 14 days refrigeration, and 30 minutes at water 40 degrees +14 days refrigeration + 500 mg/l gibberellic acid.
Results: The results showed that the highest germination percentage (70.67%), germination rate (24.26%) and germination index (246) were achieved in 750 mg/l Gibberellic acid, and the highest epicotyl length (5.09 cm), seedling length (6.23 cm) and seedling fresh weight (0.57 gr) were produced with 500 mg/l Gibberellic acid. Also, the lowest mean germination time (MGT) (4.12s day) was obtained in 1000 mg/l Gibberellic acid treatment. Also, 7 days of refrigeration + gibberellic acid at 500 mg/l treatment had no significant difference with the other concentrations of gibberellin treatments. Therefore, low gibberellin concentration with 7 days of prechilling had the same effect as higher gibberellic acid concentrations. In addition, 14 days of refrigeration + 1000 mg/l gibberellic acid treatment and treatment of 30 min in water at 40 ° C +14 days prechilling + 500 mg/l gibberellic acid produced the lowest shoot (0.78, 0.82 cm), seedling height (0.99, 1.04 cm) and fresh weight (0.013, 0.01 gr) that showed the negative effects of increased prechilling time and water at 40 ° C,
Conclusion: It was finally found that the lavender seed dormancy type is physiologically non-deep and moderate and Gibberellic acid is a suitable substitute for prechilling. The best treatment was gibberellic acid with a concentration of 750 mg/l to break the dormancy of lavender seeds.
Highlights:
- Gibberellic acid is a good substitute for prechilling in seed dormancy elimination of lavender.
- Breaking the dormancy of the lavender seeds is necessary for producing seedlings from the seeds of this plant.
Article number: 10
Type of Study: Research |
Subject:
Seed Physiology
Received: 2020/03/4 | Revised: 2024/02/21 | Accepted: 2020/09/19 | ePublished: 2022/12/11
Received: 2020/03/4 | Revised: 2024/02/21 | Accepted: 2020/09/19 | ePublished: 2022/12/11
References
1. Al-Absi, K.M. 2010. The effects of different pre-sowing seed treatments on breaking the dormancy of Mahaleb cherries, (Prunus mahaleb L.) seeds. Seed Science and Technology, 38(2): 332-340. [DOI:10.15258/sst.2010.38.2.06]
2. Arin, L. and Kiyak, Y. 2003. The effects of pre-sowing treatments on emergence and seedling growth of tomato seed (Lycopersicon esculentum Mill.) under several stress conditions. Pakistan Journal of Biological Sciences, 6(11): 990-994. [DOI:10.3923/pjbs.2003.990.994]
3. Asaadi, A.M. and Heshmati Gh.A. 2015. The effect of different treatments on breaking seeds dormancy and inducing germination of Thymus transcaucasicus Ronn. and Zataria moltiflora Boiss. Journal of Plant Research, 28(1): 12-22. [DOI:10.33762/bagrs.2015.124591]
4. Bakrim, A., Lamhamdi, M., Sayah, F. and Chibi, F. 2007. Effects of plant hormones and 20 hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedlings growth. African Journal of Biotechnology, 6(24): 2792-2802. [DOI:10.5897/AJB2007.000-2446]
5. Baskin, C.C. and Baskin, J.M. 2014. Seeds: Ecology, Biogeography and Evolution of Dormancy and Germination. Second edition. San Diego: Elsevier/Academic Press. 1600p.
6. Baskin, J.M. and C.C. Baskin. 2004. A classification system for seed dormancy. Seed Science Research, 14: 1-16. [DOI:10.1079/SSR2003150]
7. Chavagnat, A. 1978. Lavender seed dormancy and germination. In Symposium on Seed Problems in Horticulture 83: 147-154. [DOI:10.17660/ActaHortic.1978.83.19]
8. Cheraghi, F. 2011. Investigation of seed breakage and priming treatments on germination and establishment of medicinal plant (Heracleum persicum Desf) M.Sc. dissertation, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Birjand University. [In Persian with English Summary].
9. Chetouani, M., Mzabri, I., Aamar, A., Boukroute, A., Kouddane, N. and Berrichi, A. 2017. Effect of gibberellic acid (AG3) on the germination of seeds of Thymus satureioides L. and Lavandula dentata. Journal of Materials and Environmental Sciences, 8(3): 942-948.
10. Edzard, E. 2009. The efficacy of herbal medicine an overview. Fundam Clim Pharmacol, 19: 405- 410. [DOI:10.1111/j.1472-8206.2005.00335.x] [PMID]
11. Farahudi, R. and Mekizadeh Tafti, M. 2013. Study on seed breaking dormancy of Kelussia odoratissma seeds under the influence of gibberellic acid and cold treatments. Iranian Journal of Seed Science and Technology, 3(2): 249-241. [In Persian with English Summary].
12. Faryabi, A. 2011. Evaluation of the effect of different treatments on seed breakage of two medicinal plants of Yarrow and Thyme. First National Conference on Modern Issues in Agriculture, Islamic Azad University, Saveh Branch. [In Persian with English Summary].
13. Gharehmatrossian, S., Popov, Y. and Ghorbanli, M. 2014. Seed germination, dormancy breaking techniques of Citrullus colocynthis (L.) Schrab plant. Iranian Journal of Plant Physiology, 4(4): 1167-1171. [In Persian with English Summary].
14. Ghasemi Aryan, A.R., Ghorbani, R., Nasseri pourizadi, M.T. and Mesdaghi, M. 2016. Investigation of the effect of temperature on germination properties of Dorema ammoniacum. Journal of Plant Research (Iranian Journal of Biology), 29(3): 692-686. [In Persian with English Summary].
15. Gonzlez-Benito, M.E., Albert, M.J., Iriondo, J.M., Varela F. and Pérez-Garca, F. 2004. Seed germination of four thyme species after conservation at low temperatures at several moisture contents. P: 247-254. ISTA. Online - International Seed Testing Association. [DOI:10.15258/sst.2004.32.1.28]
16. Hoseini, Z. 2015. Effect of different treatments on seed breaking dormancy and increased seed germination efficiency of Echinophora platyloba, M.Sc. dissertation, Department of Seed Science and Technology, Faculty of Natural Resources and Earth Sciences, Shahrekord University, 94p. [In Persian with English Summary].
17. Hossienpour-Ggazviniy, A.A., Alizadeh, M.A., Jafari, A.A. and Valadabadi, A.R. 2011. Effect of scarification, cold and after-ripening treatments on seed dormancy breaking in four species of Satureja by standard germination test. Iranian Journal of Medicinal and Aromatic Plants, 28(1): 48-58. [In Persian with English Summary].
18. Imani, A., Rasouli, M., Tavakoli, R., Zarifi, E., Fatahi, R., Barba-Espin, G. and Martinez-Gomez, P. 2011. Optimization of seed germination in Prunus species combining hydrogen peroxide or gibberellic acid pre-treatments with stratification. Seed Science and Technology, 39(1): 204-207. [DOI:10.15258/sst.2011.39.1.18]
19. Kaneko, M., Itoh, H., Ueguchi-Tanaka, M., Ashikari M. and Matsuoka, M. 2002. The α-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in Epithelium. Plant Physiology, 128(4): 1264-1270. [DOI:10.1104/pp.010785] [PMID] [PMCID]
20. Kermode, A. R., Xia, J.H. and Schmitz, N. 2001. Dormancy of yellow cedar seeds is terminated by gibberellic acid in combination with fluridone or with osmotic priming and moist chilling. Seed Science and Technology, 29(2): 331-346.
21. Kettenring, K.M. and Galatowitsch, S.M. 2007. Temperature requirements for dormancy break and seed germination vary greatly among 14 wetland Carex species. Aquatic Botany, 87(3): 209-220. [DOI:10.1016/j.aquabot.2007.06.003]
22. Koyuncu, F. 2005. Breaking dormancy in black mulberry (Morus nigra L.) by cold stratification and exogenous application of Gibberellic Acid. Acta Biologyca Cracoviencia Botanica, 47(2): 23-26.
23. Lotfi, M., Oveisi, M., Rahimian Mashhadi, H., Pourmorad Kaleibar , B. and Naeimi , M.H. 2020. Effect of chilling time and gibberellic acid treatments on germination thermal parameters of Eryngium caeruleum. Iranian Journal of Field Crop Science, 51(1): 91-100. [In Persian with English Summary].
24. Mehrabi, I.A. and Hajinia, S. 2019. Effect of seed pre-treatments on improving germination of Astragalus gossypinum. Iranian Journal of Seed Research, 6(1): 113-95. [In Persian with English Summary]. [DOI:10.29252/yujs.6.1.95]
25. Mekizadeh Tafti, M., Farhudi, R., Rastifar, M. and Asillan, K. 2011. Methods of seed breaking dormancy ofCapparis spinosa L. Iranian Journal of Rangeland and Desert Research, 18(4): 577-569. [In Persian with English Summary].
26. Mirzadeh Wafeghi, S.A. and Nasiri, M. 2013. Effect of physical and chemical factors on seed germination of Crataegus assadii. Journal of Plant Research (Iranian Journal of Biology), 26(3): 374-366. [In Persian with English Summary].
27. Mohammadi, A.H., Jalali Honarmand, Q., Mohammad Khah, A. and Ahmadi, Gh. 2011. Seed germination. Agricultural Education and Promotion Publications, 252p. [In Persian].
28. Nabaie, M., Roshandel, P. and Mohammad Khani, A.R. 2011. Effective methods for breaking dormancy and increasing seed germination of Rheum ribes L. Iranian Journal of Medicinal and Aromatic Plants Research, 27(2): 223-212. [In Persian with English Summary].
29. Nasooti, N., Arab, M., Najafi, F. and Sahar Khiz, M. J. 2009. Evaluation of the effect of different treatments on seed germination stimulation in Lavandula angustifolia. 6th Iranian Congress of Horticultural Sciences. Rasht-Iran. [In Persian with English Summary].
30. Nemati, A., Sharifi, H., Gerdakaneh, M. and Sharifi, Z. 2016. The effect of pre-chilling and gibberellic acid on breaking seed dormancy of two medicinal plants species Silybum Mrianum and Citrulus Colocynthis. Iranian Journal of Seed Research, 3(1): 169-177. [In Persian with English Summary].
31. Nickfarjam, M., Parvin, N. and Asarzadegan, N. 2010. The effect of Lavandula angustifolia in the treatment of mild to moderate depression. Journal of Shahrekord University of Medical Sciences, 11(4): 66-73. [In Persian with English Summary].
32. Nowruzian, A., Masoumian, M., Ebrahimi M.A. and Bakhshi khaniki G.R. 2017. Effect of Breaking Dormancy Treatments on Germination of Ferula assa-foetida Seed. Iranian Journal of Seed Research; 3(2): 155-169. [In Persian with English Summary]. [DOI:10.29252/yujs.3.2.155]
33. Omidbeigi, R. 2004. Medicinal Plants Production and Processing Approaches. Astan Qods Razavi Publications of Mashhad, Volume III, Third Edition. [In Persian].
34. Paiam Noor, V. and Kordalivand, A. 2016. Effect of different seed breaking dormancy treatments on germination and yield of Betula pendula seeds. Journal of Plant Research (Iranian Journal of Biology), 29(2): 318-309. [In Persian with English Summary].
35. Parvin, P., Khezri, M., Tavasolian, I. and Hosseini, H. 2015. The effect of gibberellic acid and chilling stratification on seed germination of eastern black walnut (Juglans nigra L.). Journal of Nuts, 6(1): 67-76. [In Persian with English Summary].
36. Peivandi, M., Kazemi, L. and Majd, A. 2015. The effect of different cytokines on the micro propagation of Lavandula vera. Journal of Plant Research (Iranian Journal of Biology), 28(2): 263-257. [In Persian with English Summary].
37. Rajabian, T., Saboora, A., Hassani, B. and Fallah Hosseini, H. 2007. Effects of GA3 and chilling on seed germination of Ferula assa-foetida, as a medicinal plant. Iranian Journal of Medicinal and Aromatic Plants, 23(3): 396-403. [In Persian with English Summary].
38. Ranal, M.A., Santana, D.G., Ferreira, W.R. and Mendes-Rodrigues, C. 2009. Calculating germination measurements and organizing spreadsheets. Brazilian Journal of Botany, 32: 849-55. [DOI:10.1590/S0100-84042009000400022]
39. Ranal, M. and Santana, D. 2006. How and why to measure the germination process? Brazilian Journal of Botany, 29: 1-11. [DOI:10.1590/S0100-84042006000100002]
40. Sánchez-Gras, M.C. and Del Carmen Calvo, M. 1996. Micro propagation of Lavandula latifolia through nodal bud culture of mature plants. Plant Cell, Tissue and Organ Culture, 45: 259- 261. [DOI:10.1007/BF00043639]
41. Scott, S., Jones, R. and Williams, W. 1984. Review of data analysis methods for seed germination. Crop Science, 24: 1192-1199. [DOI:10.2135/cropsci1984.0011183X002400060043x]
42. Sekhavati, N., Hosseini, S.M., Akbarinia, M. And Rezai, A. 2011. Gibberellic and chilling for seed breaking dormancy and increased germination of Cerasus mahaleb L. Mill seed. Genetic Research and Breeding of Rangeland and Forest Plants of Iran, 19(1): 204-192.
43. Shahmoradi, Sh., Chaichi, M.R., Mozaffari, J., Mazaheri, D. and Sharifzadeh, F. 2014. Seed breaking dormancy in Hordeum spontaneum L. Journal of Plant Research (Iranian Journal of Biology), 27(5): 883-872. [In Persian with English Summary].
44. Sharifi, H. 2013. Investigation of seed dormancy and germination characteristics on thirty species of medicinal plants grown in Lorestan Province. M.Sc. dissertation, Ferdowsi University of Mashhad, Iran. [In Persian with English Summary].
45. Sharifi, H. and Goldani. M. 2016. Effect of seed coat color and different treatments on seeds dormancy and germination characteristics of mustard (Sinapis arvensis L.). Iranian Journal of Seed Research, 2(2): 47-57. [In Persian with English Summary].
46. Sharifi, H., Khajeh-Hosseini, M. and Rashed-Mohassel, M.H. 2015. Study of seed dormancy in seven medicinal species from apiaceae. Iranian Journal of Seed Research, 2(1): 25-36. [In Persian with English Summary].
47. Sharifi, H., Nemati, A. and Gordakane, M. 2017. Breaking the dormancy and improving germination of four medicinal plants from Umbrella under the influence of gibberellic acid and cold treatments. Iranian Seed Science and Research, 4(3): 38-27. [In Persian with English Summary].
48. Soyler, D. and Khawar, K.M. 2007. Seed germination of caper (Capparis ovata var. Herbacea) using K naphthalene acetic acid and gibberellic acid. International Journal of Agriculture and Biology, 9(1): 35-38.
49. Suraki, A.S., Husseini, Z. and Falah, S. 2019. Effect of chilling and its combination with gibberellic acid on the dormancy of echinophora platyloba seeds. Iranian Journal of Seed Research. 5(2): 92-104. [In Persian with English Summary]. [DOI:10.29252/yujs.5.2.91]
50. Tajbakhsh, M. 2006. Seeds (certification and its control). Ahraz Tabriz Publications. 182p. [In Persian].
51. Tipirdamaz, R. and Gomurgen, N. 2000. The effects of temperature and gibberellic acid on germination of Eranthis hyemalis Salisb. Turkish Journal of Botany, 24: 143-145.
52. Valizadeh Kaji, B. and Abbasifar, A.R. 2018. The effects of plant growth regulators and stratification on seed dormancy breaking and parameters of morphological, physiological and biochemical of seedlings of Persian walnut (Juglans regia L.). Journal of Herbal Medicine (Iranian Journal of Biology), 31(1): 92-103. [In Persian with English Summary].
53. Valizadeh, M. and Moghadam, M. 2015. Experimental design in agriculture. Publisher: Prior, Tehran, Iran. [In Persian].
54. Yamaguchi, S. and Kamiya, Y. 2000. Gibberellin biosynthesis: its regulation by endogenous and environmental signals. Plant Cell Physiology, 41(3): 251-257. [DOI:10.1093/pcp/41.3.251] [PMID]
55. Zengui, M. and Parsa, S. 2015.The effect of different seed breaking dormancy methods on seed germination of coma. Journal of Seed Ecophysiology, 1(1): 28-17.
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