Volume 8, Issue 1 ((Spring and Summer) 2021)                   Iranian J. Seed Res. 2021, 8(1): 37-53 | Back to browse issues page

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Gorzin M, Ghaderi-Far F, Sadeghipour H R, Zeinali E. Effect of Temperature and Light Regimes on Germination Behavior of Rapeseed (Brassica napus) Cultivars. Iranian J. Seed Res.. 2021; 8 (1) :37-53
URL: http://yujs.yu.ac.ir/jisr/article-1-452-en.html
Gorgan University of Agricultural Science and Natural Resources , Farshidghaderifar@gau.ac.ir
Abstract:   (1541 Views)
Extended abstract
Introduction: Since the maximum percentage and rate of germination of rapeseed occur at a certain temperature, finding these temperatures can play an important role in determining the appropriate time and place for the cultivation of different cultivars. Also, light can affect the germination percentage of rapeseed at different temperatures, but the response of rapeseed to light, especially at lower and higher temperatures, has not been studied. Therefore, this study aimed to investigate the changes in the germination of rapeseed cultivars at different temperatures and determine cardinal germination temperatures based on germination percentage and rate under both the presence and absence of light conditions.
Materials and methods: In this study, germination tests were carried out at 5, 10, 15, 20, 25, 30, 35, 37, and 40°C temperatures in two light conditions (12 h light / 12 h dark) and darkness on nine spring cultivars (Traper, Agamax, Hayola-50, Hayola-420, RGS, Mahtab, Hayola-61, Zafar, and Zarfam) and one winter cultivar (Garo). The four-parameter Hill model was used to describe germination changes over time and the dent model was used to calculate cardinal temperatures. Seed viability at lower and higher temperatures was evaluated by the tetrazolium test.
Results: The evaluation of the trend of cumulative germination percentage over time in different cultivars showed that maximum germination percentage of all cultivars happened in the temperature range between 15-30 °C, some in the temperature range of 10-30 °C (Hyola-61) and others even in the temperature range of 5-30 °C (RGS, Mahtab, Garo, Zafar, and Zarfam) had the highest germination percentage. The highest germination rate in all cultivars was observed at the temperature range of 22-35 °C. Light only had an effect on the germination percentage of the seeds at sub and super optimal temperatures. At these temperatures, light increased the germination percentage. The remaining seed of 5, 10, 35, 37, and 40 °C temperature after transfer to 20 °C did not germinate, whereas most of them were viable based on the tetrazolium test.
Conclusion: The difference in the optimum temperature range for germination percentage and rate showed that to optimize seed performance, the optimal temperature range between the germination percentage and germination rate should be considered as the optimum temperature for germination. At sub and supra optimal temperatures, light leads to improved germination in some cultivars. The effect of light on germination at supra optimal temperatures was far higher than that of sub-optimal ones. Survival of the remaining seeds at the sub and supra optimal temperatures in some cultivars provided evidence of thermo-dormancy in these cultivars, this issue needs further investigation in the future.

1- The cardinal temperatures were studied based on both the percentage and rate of germination and the effect of light on them.
2- Some new varieties such as Traper and Agamax that little information about their characteristics is available were examined.
3- In this study, the reason for the lack of germination of rapeseed at the sub and supra optimal temperatures especially in the darkness has been mentioned.
Article number: 3
Full-Text [PDF 1233 kb]   (292 Downloads)    
Type of Study: Research | Subject: Seed Ecology
Received: 2019/11/23 | Accepted: 2020/04/14

1. Akram-Ghaderi, F., Soltani, A. and Sadeghipour, H.R. 2008. Cardinal temperature of germination in medicinal pumpkin (Cucurbita pepo comvar. pepo var. styriaca), borago (Borago officinalis L.) and black cumin (Nigella sativa L.). Asian Journal of Plant Sciences, 7(6): 574-578. [In Persian with English Summary]. [DOI:10.3923/ajps.2008.574.578]
2. Ansari, O., Gherekhloo, J., Kamkar, B. and Ghaderidar, F. 2016. Breaking seed dormancy and determining cardinal temperatures for Malva sylvestris using nonlinear regression. Seed Science and Technology, 44(3): 447-460. [DOI:10.15258/sst.2016.44.3.05]
3. Bagherifard, A., Bagheri, B., Saborifard, H. and Bagherifard, G. 2014. Evaluation of cardinal temperature for three species of medicinal plants, Thymus transcaspicus, Foeniculum vulgare and Calligonum junceum. International Journal of Advanced Biological and Biomedical Research, 2(4): 482-488.
4. Bewley, J.D., Bradford, K.J., Hilhorst, H.W.M. and Nonogaki, H. 2013. Seeds (Physiology of Development, Germination and Dormancy). Third edition. New York: Springer. 392p. [DOI:10.1007/978-1-4614-4693-4]
5. Bradford, K.J. 2002. Application of hydrothermal time to quantifying and modeling seed germination and dormancy. Weed Science, 50(2): 248-260. [DOI:10.1614/0043-1745(2002)050[0248:AOHTTQ]2.0.CO;2]
6. Derakhshan, A., Gherekhloo, J., Vidal, R.A. and De Prado, R. 2014. Quantitative description of the germination of littleseed canarygrass (Phalaris minor) in Response to Temperature. Weed Science, 62(2): 250-257. [DOI:10.1614/WS-D-13-00055.1]
7. Diyanat, M. and Hosseini, S.M. 2016. Estimating cardinal temperatures and effect of different levels of temperature on germination indices of Redstem Filaree (Erodium cicutarium L.). Iranian Journal of Seed Research, 3(1): 159-168. [In Persian with English Summary].
8. Etesami, M., Rahemi Karizaki, A. and Torabi, B. 2015. Quantifying germination response of Hibiscus Tea (Hibiscus sabdariffa) seeds to temperature. Iranian Journal of Seed Research, 2(1): 73-81. [In Persian with English Summary].
9. Fallahi, H.R., Mohammadi, M., Aghhavani-Shajari, M. and Ranjbar, F. 2015. Determination of germination cardinal temperatures in two basil (Ocimum basilicum L.) cultivars using non-linear regression models. Journal of Applied Research on Medicinal and Aromatic Plants, 2: 140-145. [DOI:10.1016/j.jarmap.2015.09.004]
10. Farzaneh, S., Soltani, E., Zeinali, E. and Ghaderi-Far, F. 2014. Screening oilseed rape germination for termo tolerance using a laboratory-based method. Seed Technology, 36(1): 15-27.
11. Finch-Savage, W.E. and Bassel, G.W. 2015. Seed vigour and crop establishment: extending performance beyond adaptation. Journal of Experimental Botany, 67(3): 567-91. [DOI:10.1093/jxb/erv490] [PMID]
12. Ghaderi-Far, F. and Gorzin, M. 2019. Applied Research in Seed Technology. Published by Gorgan University of Agricultural Science and Natural Resources, 240p.
13. Ghaderi-Far, F., Alimagham, S.M., Kameli, A.M. and Jamali, M. 2012. Isabgol (Plantago ovata Forsk) seed germination and emergence as affected by environmental factors and planting depth. International Journal of Plant Production, 6(2): 185-194.
14. Ghaderi-Far, F., Gherekhloo, J. and Alimagham, M. 2010. Influence of environmental factors on seed germination and seedling emergence of yellow sweet clover (Melilotus officinalis). Planta Daninha, 28(3): 463-469. [DOI:10.1590/S0100-83582010000300002]
15. Ghaderi-Far, F., Soltani, A. and Sadeghipour, H.R. 2009. Evaluation of nonlinear regression models in quantifying germination rate of medicinal pumpkin (Cucurbita pepo L. subsp. Pepo. Convar. Pepo var. styriaca Greb), borago (Borago officinalis L.) and black cumin (Nigella sativa L.) to temperature. Journal of Plant Production, 16(4): 1-19. [In Persian with English Summary].
16. Gruber, S., Emrich, K. and Claupein, W. 2009. Classification of canola (Brassica napus) winter cultivars by secondary dormancy. Canadian Journal of Plant Science, 89: 613-619. [DOI:10.4141/CJPS08190]
17. Heidari, Z., Kamkar, B. and Masoud Sinaki, M. 2014. Determination of cardinal temperatures of milk thistle (Silybum marianum L.) germination. Advances in Plants and Agriculture Research, 1(5): 20-27. [DOI:10.15406/apar.2014.01.00027]
18. ISTA. 2003. ISTA Working Sheets on Tetrazolium Testing. Volume 1. Published by International Seed Testing Association (ISTA). P. O. Box 308, 8303 Bassersdorf, CH-Switzerland.
19. Khaliliaqdam, N., Mirmahmoudi R. and Saeedian S. 2017. Determination of cardinal temperature of Flax seed (Linum usitatissimum L.) by Nonlinear Regression Method. Seed Research Journal, 7(2): 41-49. [In Persian with English Summary].
20. Kheirkhah, M., Kouchaki, A., Rezvani Moghadam, P. and Nasiri Mahalati, M. 2014. The determination of germination cardinal temperature of a medicinal plant perennial Ziziphora (Ziziphora clinopodiodes lam). Iranian Journal of Field Crops Research, 11(4): 543-550. [In Persian with English Summary].
21. Luo, T., Xian, M., Khan, M.N., Hu, L. and Xu, Z. 2018. Estimation of base temperature for germination of rapeseed (Brassica napus) using different models. International Journal of Agriculture and Biology, 20(3): 524-530. [DOI:10.17957/IJAB/15.0512]
22. Mamedi, A., Tavakkol Afshari, R. and Oveisi, M. 2017. Cardinal temperatures for seed germination of three Quinoa (Chenopodium quinoa Willd.) cultivars. Iranian Journal of Field Crop Science, 2: 89-100.
23. NezhadHassan, B., Siahmarguee, A., Zeinali, E., Ghaderi-Far, F. and Soltani, E. 2017. Evaluation of non linear regression models to description germination rate of Arugula (Eruca sativa Mill.) to temperature and water potential. Iranian Journal of Seed Science and Research, 4(2): 1-16. [In Persian with English Summary].
24. Piper, E.L., Boote, K.J., Jones, J.W. and Grimm, S.S. 1996. Comparison of two phenology models for predicting flowering and maturity date of soybean. Crop Science, 36(6): 1606-1614. [DOI:10.2135/cropsci1996.0011183X003600060033x]
25. Ritchie, J.T. and NeSmith, D.S. 1991. Temperature and crop development. In: Hanks, R.J., Ritchie, J.T. (eds.), Modeling Plant and Soil Systems. Agronomy Monograph, 31: 5-29. [DOI:10.2134/agronmonogr31.c2]
26. Russo, V.M., Bruton, B.D. and Sams, C.E. 2010. Classification of temperature response in germination of Brassicas. Industrial Crops Products, 31(1): 48-51. [DOI:10.1016/j.indcrop.2009.08.007]
27. Shayanfar A., Ghaderi-Far F., Behmaram R., Soltani A. and Sadeghipour, H.R. 2017. Assessment of germination and secondary dormancy behaviours of lines and cultivars of canola. Crops Improvement, 19(4): 881-892. [In Persian with English Summary].
28. Shayanfar, A., Ghaderi-Far, F., Behmaram, R., Soltani, A. and Sadeghipour, H.R. 2018. The effect of temperature and light on germination and secondary seed dormancy of rapeseed (Brassica napus L.). Journal of Plant Protection, 32(2): 269-278. [In Persian with English Summary].
29. Soltani, E., Baskin, C.C., Baskin, J.M., Soltani, A., Galeshi, S., Ghaderi-far, F. and Zeinali, E. 2016. A quantitative analysis of seed dormancy and germination in the winter annual weed Sinapis arvensis (Brassicaceae). Botany, 94(4): 289-300. [DOI:10.1139/cjb-2015-0166]
30. Soltani, E., Baskin, J.M. and Baskin, C.C. 2018. A review of the relationship between primary and secondary dormancy, with reference to the volunteer crop weed oilseed rape (Brassica napus). Weed Research, 59(1): 5-14. [DOI:10.1111/wre.12342]
31. Soltani, E., Galeshi, S., Kamkar, B. and Akramghaderi, F. 2008. Modeling seed aging effects on the response of germination to temperature in wheat. Seed Science and Biotechnology, 2(1): 32-36.
32. Stirk, W.A., Novak, O., Žižková, E., Motykac, V., Strnad, M. and van Staden, J. 2012. Comparison of endogenous cytokinins and cytokinin oxidase/dehydrogenase activity in germinating and thermoinhibited Tagetes minuta achenes. Journal of Plant Physiology, 169(7): 696-703. [DOI:10.1016/j.jplph.2012.01.013] [PMID]
33. Suanda, D.K. 2012. Cardinal temperatures of Brassica sp. and how to determine it. Agrotrop, 2(1): 33-39. [DOI:10.5010/JPB.2012.39.1.033]
34. Summerfield, R.J., Roberts, E.H., Ellis, R.H. and Lawn, R.J. 1991. Towards the reliable prediction of time to flowering in six annual crops. I. The development of simple model for fluctuating field environments. Experimental Agriculture, 27(1): 11-31. [DOI:10.1017/S0014479700019165]
35. Tolyat, M.A., Tavakol Afshari, R., Jahansoz, M.R., Nadjafi, F. and Naghdibadi, H.A. 2014. Determination of cardinal germination temperatures of two ecotypes of Thymus daenensis subsp. Daenensis. Seed Science and Technology, 42(1): 28-35. [DOI:10.15258/sst.2014.42.1.03]
36. Torabi, B., Attarzadeh, M. and Soltani, A. 2013. Germination response to temperature in different Safflower (Carthamus tinctorius) cultivars. Seed Technology, 35(1): 47-59.
37. Weber, E.A., Frick, K., Gruber, S. and Claupein, W. 2010. Research and development towards a laboratory method for testing the genotypic predisposition of oilseed rape (Brassica napus L.) to secondary dormancy. Seed Science and Technology, 38(2): 298-310. [DOI:10.15258/sst.2010.38.2.03]
38. Weber, E.A., Gruber, S., Stockmann, F. and Claupein, W. 2013. Can low-dormancy oilseed rape (Brassica napus) genotypes be used to minimize volunteer problems?. Field Crops Research, 147: 32-39. [DOI:10.1016/j.fcr.2013.03.017]
39. Yin, X., Kropff, M.J., McLaren, G. and Visperas, R.M. 1995. A nonlinear model for crop development as a function of temperature. Agricultural and Forest Meteorology, 77(1-2): 1-16. [DOI:10.1016/0168-1923(95)02236-Q]
40. Yuan, X. and Wen, B. 2018. Seed germination response to high temperature and water stress in three invasive Asteraceae weeds from Xishuangbanna, SW China. Plos One, 13(1): e0191710. [DOI:10.1371/journal.pone.0191710] [PMID] [PMCID]
41. Zeinali, E., Soltani, A., GalesHi, S. and Sadati, S.J. 2010. Cardinal temperatures, response to temperature and range of thermal tolerance for seed germination in wheat (Triticum aestivum L.) cultivars. Electronic Journal of Crop Production, 3(3): 23-42. [In Persian with English Summary].

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