Volume 4, Issue 2 ((Autumn & Winter) 2018)                   Iranian J. Seed Res. 2018, 4(2): 37-55 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghanbari M, Modarres-Sanavy S A M, Mokhtassi Bidgoli A, Talebi-Siah Saran P. Effect of hydropriming and seed aging on seed germination and biochemical characteristics of pinto bean (Phaseolus vulgaris) seed under salt stress. Iranian J. Seed Res.. 2018; 4 (2) :37-55
URL: http://yujs.yu.ac.ir/jisr/article-1-226-en.html
Tarbiat Modares University , majid.ghanbari@modares.ac.ir
Abstract:   (14458 Views)
This study was conducted to evaluate the effect of hydropriming and seed aging on germination and enzymatic properties of pinto bean under salinity stress as factorial based on a completely randomized design with four replications. Two groups of seeds (i.e., non-aged and aged seeds), two hydropriming treatments (i.e., hydro primed and unprimed seeds) and six salinity treatments (i.e., 0, 2, 4, 6, 8 and 10 dS/m) were the experimental factors. The results showed that the highest mean time and percentage of germination, plumule length and vigor were observed in the control (i.e., distilled water) and hydropriming treatments. Maximum root length and percentage of seedling water were obtained in the control (i.e., distilled water) and aged seed treatments. In addition, in terms of radicle dry weight, the highest amount was observed in salinity of 4 dS/m and non-aged hydro primed seeds. The highest plumule dry weight was observed in salinity of 6 dS/m and allometric index in salinity of 8 dS/m for non-aged seed and aged seed without hydropriming, respectively. An increase in the levels of salinity stress and aging the seeds increased the malondialdehyde and reduced the activity of germination, mean time and germination percentage, and seedling growth. Seed hydropriming reduced the peroxidation of the cell membrane and generally improved the speed and uniformity of germination, aged and natural seeds vigor under both salinity and optimum conditions. As a result, hydropriming can increase the tolerance of bean seeds to salinity at the germination stage and increase the germination capacity of stored seeds for cultivation.
  1. The effect of hydropriming on recovering the power of seed germination of pinto bean has been studied under the salinity condition.
  2. An attempt has been made to determine the effect of hydropriming on increasing the tolerance of pinto bean seeds to storage and salinity stress condition.
  3. The effect of pinto bean storage on biochemical changes and enzyme activity has been studied in salinity stress condition.
Full-Text [PDF 454 kb]   (2267 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2017/03/3 | Accepted: 2018/01/29

1. Aghabarati, A., and Maralian, H. 2011. The effect of seed deterioration on germination and vigor of maple Acer cineracens Boiss. Quarterly Journal of Natural Ecosystems Iran, 2(2): 25-35. [In Persian with English Summary].
2. Agrawal, R. 2003. Seed technology. Pub. Co. PVT. LTD. New Delhi, India. 829p.
3. Ahmadloo, F., Tabari, M., and Behtari, B. 2012. Effect of water stress and accelerated ageing on some physiological characteristics of Pinus brutia Ten. seeds. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 19(2): 345-358. [In Persian with English Summary].
4. Ahmadpoor Dehkordi, S., and Balouchi, H.R. 2013. Effect of seed priming on antioxidant enzymes and lipids peroxidation of cell membrane in Black cumin (Nigella sativa) seedling under salinity and drought stress. Journal of Crop Production, 5(4): 63-85. [In Persian with English Summary].
5. Artola, A., Carrillo-Castaneda, G., and Santos, G.D.L. 2003. Hydropriming: A strategy to increase Lotus Corniculatus L. Seed vigor. Seed Science and Technology, 31: 455-463. [DOI:10.15258/sst.2003.31.2.22]
6. Azarnivand, H., Zandi Esfahan, E., and Shahriary, E. 2005. Effect of salinity stress on germination of Haloxylon aphyllum, Seidlitzia rosmarinus and Hammada salicornica. Journal of Desert, 11(1): 187-196. [In Persian with English Summary].
7. Bailly., C. 2004. Active oxygen species and antioxidants in seed biology. Seed Science Research, 14(2): 93-107. [DOI:10.1079/SSR2004159]
8. Basra, S.M.A., Ahmad, N., Khan, M.M., Iqbal, N., and Cheema, M.A. 2003. Assessment of cottonseed deterioration during accelerated aging. Seed Science and Technology, 31(3): 531-540. [DOI:10.15258/sst.2003.31.3.02]
9. Basra, S.M.A., Rehman, K.U., and Iqbal, S. 2000. Cottonseed deterioration: assessment of some physiological and biochemical aspects. International Journal of Agriculture and Biology, 2(3), 195-198.
10. Bernfeld, P. 1970. Amylase α and β, in methods in Enzymology. In: S. Colowick and N. Kaplan (Eds). Academic Press, New York, p. 149.
11. Bewley, D.J. 1997. Seed germination and dormancy. Plant Cell, 9(7): 1055-1060. [DOI:10.1105/tpc.9.7.1055] [PMID] [PMCID]
12. Chojnowski, F.C., and Come, D. 1997. Physiology and biochemical changes induced in sunflower seeds by osmopriming and drying, storage and aging. Seed Science Research, 7(4): 323-331. [DOI:10.1017/S096025850000372X]
13. Cobley, L.S. 1996. An introduction to the botany of tropical crops. New York, London, Longman. 88-91.
14. Da Silva, E.A.A., Toorop, P.E., Nijsse, J., Bewley, J.D., and Hilhorst, H.W.M. 2005. Exogenous gibberellins inhibit coffee (Coffea arabica L. cv. Rubi) seed germination and cause cell death in the embryo. Journal of Experimental Botany, 56(413): 1029-1038. [DOI:10.1093/jxb/eri096] [PMID]
15. Dai, S., Wang, T., Yan, X., and Chen, S. 2007. Proteomics of pollen development and germination. Journal of Proteome Research, 6(12): 4556-4563. [DOI:10.1021/pr070474y] [PMID]
16. Dell Aquila, A., and Diturin, M. 1996. The germination response to heat and salt stress in evaluation vigor loss in aged wheat seeds. Seed Science and Technology, 24: 309-319.
17. Dkhil, B.B., and Denden, M. 2010. Salt stress induced changes in germination, sugars, starch and enzyme of carbohydrate metabolism in Abelmoschus esculentus L. (Moench.) seeds. African Journal of Agricultural Research, 5(12): 1412-1418.
18. Ehsanullah, K., and Smith, M.I. 2002. Soaking injury in relation to the rate of imbibitions in soybean (Glycine max L. Merrill). Pakistan Journal of Seed Science and Technology, 1(1): 27-33.
19. Eisvand, H.R., and Alizadeh, M.A. 2002. The assessment of some physiological factors badrashbo (Dracocephalum moldavica) medicinal plant seed test under the terms of premature aging. Genetic Research and Correction of Forest and Pasture Plants in Iran, 11(2): 249-255. [In Persian with English Summary].
20. Eisvand, H.R., and Maddah Arefi, H. 2007. Effects of some plant growth regulators on the physiological quality of Bromus aged seed. Iranian Journal of Rangelands and Forest Plant Breeding and Genetic Research, 15(2): 159-171. [In Persian with English Summary].
21. Eisvand, H.R., Tavakkol Afshari, R., Sharifzadeh, F., Arefi, H.M., and Hesamzadeh Hejazi, S.M. 2008. Improve the physiological quality of the seeds of decline in the tall wheatgrass (Agropyron elongatum Host) using hormonal priming for stress and non-stress conditions. Iranian Journal of Field Crop Science, 39(1): 53-65. [In Persian with English Summary].
22. Ellis, R.H., hory, T.P., and Roberts, E.H. 1980. Towards a rational basis for testing seed quality. Proceedings-Easter School of Agricultural Science, University of Nottingham, Butterworths, London. 605-635.
23. Fallah, A., and Babaei, M. 2006. The assessment of salinity stress on germination of rice. Journal of Agricultural Sciences and Natural Resources, 4: 12-18. [In Persian with English Summary].
24. Farhoudi, R. 2006. Effect of salinity stress on alpha-amylase activity, cell membrane leakage and seedling growth of canola cultivars. Journal of Process and Plant Function, 1(1): 14-25. [In Persian with English Summary].
25. Farooq, M., Basra, S.M.A., Warraich, E.A., and Khaliq, A. 2006. Optimization of hydropriming techniques for rice seed invigoration. Seed Science and Technology, 34(2): 529-534. [DOI:10.15258/sst.2006.34.2.25]
26. Fowler, D.B., Gusta, L.V., and Tyler, N.J. 1981. Selection for winter hardiness in wheat. III. Screening methods. Crop Science, 21(6): 896-901. [DOI:10.2135/cropsci1981.0011183X002100060023x]
27. Ghanbari, M., and Karamnia, S. 2016. Evaluation of the effect of seed aging on some characteristics of bean germination (Phaseolus vulgaris L.) landraces of Guilan province under salinity stress conditions. The 6th Iranian Pulse Crops Symposium, 4 May, Khoram-abad. [In Persian Summary].
28. Ghanbari, M., Mansour Ghanaei Pashaki, K., Safaei Abdolmanaf, S., and Aziz Ali-abadi, K. 2016. Effect of salt stress and hydropriming on germination characteristics of Mungbean (Vigna radiata (L.) Wilczek). Iranian Journal of Pulses Research, 7(1): 65-80. [In Persian with English Summary].
29. Ghasemi Golazani, K., Mohammadian, R., Moghaddam, R. and Sadegheian, Y. 1996. Effect of seed aging on germination and seedling growth had seven sugar beet breeding populations under salinity treatments. Journal of Agricultural Sciences and Natural Resources, 2(4): 39-43. [In Persian with English Summary].
30. Gholami Tileh-boni, H., Salehi Balashahri, M., and Farhadi, R. 2012. The effect of Priming and deterioration of seed germination and seedling growth changes of rice (Oryza sativa L.). Seed Science and Technology, 2(1): 1-13. [In Persian with English Summary].
31. Hamedi, S.E., and Bakhtiari, S. 2011. Effect of Hydro and Haloperiming on seed germination and seedling growth of red bean (Phaseoluse vulgaris L.) under salt stress. The 2nd National Conference on Seed Science and Technology. Islamic Azad University, Mashhad Branch. [In Persian with English Summary].
32. Harris, D., Tripathi, R.S., and Joshi, A. 2000. On-farm seed priming to improve crop establishment and yield in direct-seeded rice. In, IRRI: International Workshop on Dry-seeded Rice Technology, held in Bangkok, 25-28 January 2000. International Rice Research Institute, Manila, Philippines. 164p.
33. Hassanzadeh Kahal Sofla, S., Taheri, G., and Mehrzad, J. 2012. Priming effects on germination of sweet corn (Zea mays cv. Basin) under sodium chloride stress. Seed Science and Technology, 2(1): 62-70. [In Persian with English Summary].
34. Hopkins, R.O., Waldram, K., and Kesner, R.P. 2004. Sequences assessed by declarative and procedural tests of memory in amnesic patients with hippocampal damage. Neuropsychologia, 42(14): 1877-1886. [DOI:10.1016/j.neuropsychologia.2004.05.008] [PMID]
35. Hosseini, F., Siadat, S.A., Bakhshandeh, A.M., and Chab, A.N. 2011. Evaluate the effect of oxygen tension on germination and seedling growth of five components of wheat. Iranian Journal of Field Crops Research, 9(4): 631-638. [In Persian with English Summary].
36. ISTA. 2004. International Rules for Seed Testing. International Seed Testing Association. Zurich. Switzerland.
37. Judi, S., and Sharizadeh, F. 2004. Investigation of hydropriming effects on barley cultivars. Journal of Desert, 11(1): 99-109.
38. Kaur, S., Gupta, A.K., and Kaur, N. 2002. Effect of osmo and hydro priming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress. Plant Growth Regulation, 37(1): 17-22. https://doi.org/10.1023/A:1020355505105 [DOI:10.1023/A:1020310008830]
39. Kaur, S., Gupta, A.K., and Kaur, N. 2003. Priming of chickpea seeds with water and Mannitol overcomes the effect of salt stress on seedling growth. International Chickpea and Pigeon pea Newsletter, 10: 18-20.
40. Khajeh-Hosseini, M., Powell, A.A., and Bingham, I.J. 2003. The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Science and Technology, 31(3): 715-725. [DOI:10.15258/sst.2003.31.3.20]
41. Khalesro, Sh., and Aghaalikhani, M. 2007. Effect of salinity and water Deficit stress on seed germination. Pajouhesh & Sazandegi. 77: 153-163. [In Persian with English Summary].
42. Khalili Eghdam, N., and Gorzin, O. 2012. Effect of deterioration on the discharge of seeds and growth of heterotrophic soybean seedlings. Journal of Seed Science and Technology, 1(1): 29-33. [In Persian with English Summary].
43. Kochaki, A., and Zarif Ketabi, H. 1996. Determine the optimum temperature for germination and monitored for salinity and drought effects of several range species. Journal of Desert, 1(1): 24-36. [In Persian with English Summary].
44. Mahdavi, M. 2005. Applied hydrology. Tehran University Press. 362p. [In Persian]. [PMCID]
45. Mashi, A., and Galeshi, S. 2007. The effect of salinity on germination indexes of four Hull-less barley genotypes. Journal of Agricultural Sciences and Natural Resources, 13(6): 45-57. [In Persian with English Summary].
46. McDonald, M.B. 2000. Seed priming. In Black, M., and Bewley, J.D. (ed.). Seed technology and Its Biological Basis. Sheffield Acad. Press, Sheffield, England. 287-326.
47. Noctor, B., and Foyer, C.H. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Annual Review Plant Physiology, 49(1): 249-279. [DOI:10.1146/annurev.arplant.49.1.249] [PMID]
48. Parsa, M., and Bagheri, A.R. 2013. Legumes. Mashhad Jihad University Press, 528P. [In Persian].
49. Rajabi, R., and Postini, K. 2005. Effects of NaCl on thirty cultivars of bread wheat seed germination. Agriculture Science Journal, 27: 29-45.
50. Redmann, R.E., Ql, M.Q., and Belyk, M. 1994. Growth of transgenic and standard canola (Brassica napus L.) varieties in response to soil salinity. Plant Science, 74(4): 797-799. [DOI:10.4141/cjps94-142]
51. Salim, M. 1991. Comparative growth responses and ionic relations of four cereals during salt stress. Journal of Agronomy and Crop Science, 166(3): 204-209. [DOI:10.1111/j.1439-037X.1991.tb00905.x]
52. Samiei, D. 2000. Green beans. Promotion magazine, Production office and promotion and technical Publishing Department of the Ministry of Jihad-e-Keshavarzi. [In Persian].
53. Shoeoan, I.S., and Garo, O.P. 1985. Effect of different types of salinities during germination: Seedling growth and water relation. Indian Journal of Plant Physiology, 26: 263-369.
54. Sivritepe, N., Sivritepe, H.O., and Eris, A. 2003.The effects of NaCl priming on salt tolerance in melon seedling grown under saline conditions. Scientia Holticuturae, 97(3-4): 229-237. [DOI:10.1016/S0304-4238(02)00198-X]
55. Soltani, A., Galeshi, S., Zeinali, E., and Latifi, N. 2001. Genetic variation for and interrelationships among seed vigor traits in wheat from the Caspian Sea coasts of Iran. Seed Science and Technology, 29: 653-662.
56. Soltani, A., Gholipoor, M., and Zeinali, E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany, 55(1-2): 195-200. [DOI:10.1016/j.envexpbot.2004.10.012]
57. Soltani, E., Kamkar, B., Galeshi, S., and Akram Ghaderi, F. 2008. The effect of seed deterioration on seed reserves depletion and heterotrophic seedling growth of wheat. Journal of Agricultural Sciences and Natural Resources, 15(1): 182-193. [In Persian with English Summary].
58. Sreenivasulu, N., Grimm, B., Wobns, U., and Weschke, W. 2000. Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedling of foxtail millet. Physiology Plantarum, 109: 435-442. [DOI:10.1034/j.1399-3054.2000.100410.x]
59. Srinivasan, K., and Saxena, S. 2001. Priming seeds for improved viability and storability in Raphanus sativus cv. Chinese Pink. Indian Journal of Plant Physiology, 6: 271-274.
60. Tamartash, R., Shokrian, F., and Kargar, M. 2010. Effects of salinity and drought stress on Trifolium alexanderium L. seed germination properties. Rangeland, 4(2): 288-297. [In Persian with English Summary].
61. Tavili, A., Saberi, M., Jafari, M., Safari, B., and Sadeghi Sangdehi, A. 2009. Influence of salinity and temperature on germination of Trifolium alexanderinum. Journal of Plant Ecophysiology, 1(1): 18-28. [In Persian with English Summary].
62. Verma, S.S., Verma, U., and Tomer, R.P.S. 2003. Studies on seed quality parameters in deterioration seeds in Brassica (Brassica campestris). Seed Science and Technology, 31(2): 389-398. [DOI:10.15258/sst.2003.31.2.15]
63. Windauer, L., Altuna, A., and Benech-Arnold, R. 2007. Hydrotime analysis of Lesquerella fendleri seed germination responses to priming treatments. Industrial Crops and Products, 25(1): 70-74. [DOI:10.1016/j.indcrop.2006.07.004]
64. Zamani, A., Sadat Nouri, S.A., Tavakol Afshari, R., Iran Nejad, H., Akbari, Gh.A., and Tavakoli, A. 2010. Evaluation of lipid peroxidation and activity of antioxidant enzymes in safflower seed under natural and artificial aging. Iranian Journal of Crop Science, 41(3): 545-554. [In Persian with English Summary].
65. Zia, S., and Khan, M.A. 2004. Effect of light salinity and temperature on seed germination of Limonium stocksii. Canadian Journal of Botany, 82(2): 151-157. [DOI:10.1139/b03-118]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.