Volume 3, Issue 2 ((Autumn & Winter) 2017)                   Iranian J. Seed Res. 2017, 3(2): 155-169 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nowruzian A, Masoumian M, Ebrahimi M A, Bakhshi khaniki G R. Effect of Breaking Dormancy Treatments on Germination of Ferula assa-foetida Seed. Iranian J. Seed Res. 2017; 3 (2) :155-169
URL: http://yujs.yu.ac.ir/jisr/article-1-215-en.html
Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran , masoumian200@yahoo.com
Abstract:   (20168 Views)

Asafetida (Ferula assa- foetida L.) is an important medicinal plant belonging to Apiaceae family and has long dormancy. In this research, vernalization, washing time, GA3, medium strength, harvesting time and interaction of these treatments were studied to optimize condition of germination. The results showed that vernalization at 4-5°C for two weeks increased germination by 50%, as compared with the control. Maximum and minimum germinations were obtained for 6 and 2 hours’ washing, which was 42% and 20.47%, respectively. Germination of Ferula was increased (41.5%) by using 10 mg/l of GA3, as compared with the control. In addition, using half strength MS media led to a 25% increase in germination. Moreover, germination mean increased by applying these treatments to one-year-old seeds, in comparison with fresh ones (61% and 36%, respectively). By running factorial experiments in the CRD, the best combination of treatments which could significantly increase germination was a combination of vernalization (4-5°C for two weeks), half strength MS media, GA3 (10 mg/l) and washing time (6h). Given the results of the study, for the purpose of breaking the dormancy of Asafetida, it is suggested that use is made of one-year-old seeds, and half strength MS media, along with right combinations of vernalization, washing time and GA3.

Full-Text [PDF 685 kb]   (2883 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2016/04/3 | Accepted: 2016/07/27

1. Al-Absi, K.M. 2010. The effects of different pre-sowing seed treatments on breaking the dormancy of Mahaleb cherries, (Prunus mahaleb L.) seeds. Seed Science and Technology, 38(2): 332-340. [DOI:10.15258/sst.2010.38.2.06]
2. Bakrim, A., Lamhamdi, M., Sayah, F., and Chibi, F. 2007. Effects of plant hormones and 20 hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedlings growth. African Journal of Biotechnology, 6(24): 2792-2802. [DOI:10.5897/AJB2007.000-2446]
3. Baskin, J.M., and Baskin, C.C. 1998. Ecology, biogeography and evolution of dormancy and germination. Elsevier. 162-177.
4. Bewley, J.D., and Black, M., 1994. Seeds: Physiology of development and germination. Second Edition, Plenum, Press., New York. 199-271. _5 [DOI:10.1007/978-1-4899-1002-8]
5. Chiwocha, S.D., Cutler, A.J., Abrams, S.R., Ambrose, S.J., Yang, J., Ross, A.R., and Kermode, A.R. 2005. The etr1‐2 mutation in Arabidopsis thaliana affects the abscisic acid, auxin, cytokinin and gibberellin metabolic pathways during maintenance of seed dormancy, moist‐chilling and germination. The Plant Journal, 42(1): 35-48. [DOI:10.1111/j.1365-313X.2005.02359.x] [PMID]
6. Gharehmatrossian, S., Popov, Y., and Ghorbanli, M. 2014. Seed germination, dormancy breaking techniques of Citrullus colocynthis (L.) Schrab plant. Iranian Journal of Plant Physiology, 4(4): 1167-1171.
7. Golmohammadi, F. 2013. Medical plant of Ferula assa-foetida and its cultivating, main characteristics and economical importance in South khorasan province - east of Iran. Technical Journal of Engineering and Applied Sciences, 3(18): 2334-2346.
8. Hassan, F.A., and Fetouh, M.I. 2014. Seed germination criteria and seedling characteristics of Magnolia grandiflora L. trees after cold stratification treatments. International Journal of Current Microbiology and Applied Sciences, 3(3): 235-241.
9. Hassani, B., Saboora, A., Radjabian, T., and Fallah Husseini, H. 2009. Effects of temperature, GA3 and Cytokinins on breaking seed dormancy of Ferula assa-foetida L. Iranian Journal of Science and Technology (Sciences), 33(1): 75-85.
10. Imani, A., Rasouli, M., Tavakoli, R., Zarifi, E., Fatahi, R., Barba-Espin, G., and Martinez-Gomez P. 2011. Optimization of seed germination in Prunus species combining hydrogen peroxide or gibberellic acid pre-treatments with stratification. Seed Science and Technology, 39(1): 204-207. [DOI:10.15258/sst.2011.39.1.18]
11. International Seed Testing Association (ISTA), 1999. International rules for seed testing. Seed Science and Technology, (Supplement). 27: 333.
12. International Seed Testing Association (ISTA). 1996. International rules for seed testing. Seed Science and Technology. 21, (Supplement Rules).
13. Makunga, N.P., Jager, A.K., and Van Staden, J., 2005. An improved system for the in vitro regeneration of Thapsia garganica via direct organogenesis–influence of auxins and Cytokinins. Plant Cell, Tissue and Organ Culture, 82(3): 271-280. [DOI:10.1007/s11240-005-1372-x]
14. Martin, K. P., and Madassery, J. 2006. Rapid in vitro propagation of Dendrobium hybrids through direct shoot formation from foliar explants, and protocorm-like bodies. Scientia Horticulturae, 108(1): 95-99. [DOI:10.1016/j.scienta.2005.10.006]
15. Murashige, T., and Skoog, F. 1962. Arevised media for rapid growth and bioassay with tobacco tissue cultures. Physiolagia Plantarum, 15(3): 473-497. [DOI:10.1111/j.1399-3054.1962.tb08052.x]
16. Nadjafi, F., Bannayan, M., Tabrizi, L., and Rastgoo, M. 2006. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environment, 64(3): 542-547. [DOI:10.1016/j.jaridenv.2005.06.009]
17. Otroshy, M., Zamani, A., Khodambashi, M., Ebrahimi, M., and Struik, P.C. 2009. Effect of exogenous hormones and chilling on dormancy breaking of seeds of Asafoetida (Ferula assa-foetida L.). Research Journal of Seed Science, 2(1): 9-15. [DOI:10.3923/rjss.2009.9.15]
18. Parvin, P., Khezri, M., Tavasolian, I., and Hosseini, H. 2015. The effect of gibberellic acid and chilling stratification on seed germination of eastern black walnut (Juglans nigra L.). Journal of Nuts, 6(1): 67-76.
19. Raisi, A. Nabavi Kalat, S.M., and Sohani Darban, A.R. 2013. The study effects of stratification, temperature and potassium nitrate on seed dormancy breaking Ferula assa foetida. World Applied Sciences Journal, 21(3): 379-383.
20. Rawat, J.M.S., Tomar, Y.K., and Rawat, V. 2010. Effect of stratification on seed germination and seedling performance of wild pomegranate. Journal of American Science, 6(5): 97-99.
21. Slater, R.J., and Bryant, J.A. 1982. RNA metabolism during breakage of seed dormancy by low temperature treatment of fruites of Acer platanoides. Annals of Botany, 50(2): 141-149. [DOI:10.1093/oxfordjournals.aob.a086351]
22. Thomas, T.H., 1990. Hormonal involvement in photo regulation of celery seed dormancy. Monograph British Society for Plant Growth Regulation, 20: 51-59.
23. Tipirdamaz, R., and Gomurgen, N. 2000. The effects of temperature and gibberellic acid ongermination of Eranthis hyemalis (L.) salisb seeds. Turkish Journal of Botany, 24(2): 143-145.
24. Zare, A.R., Solouki, M., Omidi, M., Iravani, N., Oladzad Abasabadi, A., and Mahdi Nezad, N. 2011. Effect of various treatments on seed germination and dormancy breaking in Ferula assa foetida L. (Asafetida), a threatened medicinal herb. Trakia Journal of Sciences, 9(2): 57- 61.

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.