Volume 9, Issue 1 ((Spring and Summer) 2022)
Iranian J. Seed Res. 2022, 9(1): 111-126 |
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Sourazar K, Sedghi M, Seyed Sharifi R. (2022). The effect of priming on physiological and biochemical traits of French bean (Phaseolus vulgaris) under cobalt chloride stress. Iranian J. Seed Res.. 9(1), : 7 doi:10.52547/yujs.9.1.111
URL: http://yujs.yu.ac.ir/jisr/article-1-524-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-524-en.html
Ph.D. Student, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabilli, Ardabil, Iran. , k_sourazar@uma.ac.ir
Abstract: (2076 Views)
Extended Abstract
Introduction: The germination stage ensures the durability, establishment, and final yield of plants. The final density of plants per unit area is resulted when the planted seeds germinate fully and with adequate rate. Plants mainly undergo abiotic stresses that are a considerable constraint for agricultural production worldwide. Seed priming is one of the simplest and cheap methods to improve seed germination, acceleration of seedling growth and establishment, uniformity, reduction of flowering time, vigorous seedling production, etc which leads to improved quality and yield of crop under stressful and non-stressful conditions. This study aimed to investigate the effect of different types of priming on enzymatic and physiological changes of French bean seed under cobalt chloride stress conditions.
Materials and Methods: To examine the effect of priming on germination indices, the activity of antioxidant enzymes and proteins in French bean under cobalt chloride stress, a factorial experiment was performed in a completely randomized design with three replications. These factors included four levels: seed priming with 100 mg / l salicylic acid and mannitol, control and distilled water (no priming), and three levels of cobalt chloride stress (0, 100, and 200 mg / l).
Results: The results showed that with increasing cobalt chloride concentration, germination indices (germination rate, root length, root fresh weight, stem fresh weight and stem dry weight) decreased. However, in the pretreatment of distilled water, salicylic acid, and mannitol there was a significant increase in germination indices (mean germination time, radicle length, plumule length, fresh radicle weight, and weight plumule) was observed. The interaction effect of priming and cobalt chloride caused an increase in radicle dry weight compared to control × stress treatment. The highest values of fresh radicle weight (14.4 g), dry weight of plumule (0.27 g), and plumule length (0.17 cm) were obtained in hydro priming pretreatment. The results also showed that hormone priming under stress conditions of 200 and 100 mg / l cobalt chloride increased protein content and polyphenol oxidase activity of French bean seedlings compared with the control treatment, respectively. Osmo priming increased the activity of catalase and peroxidase enzymes under stress and non-stress conditions resulted by 200 mg / l cobalt chloride.
Conclusion: according to the results of the present study, cobalt chloride reduced some physiological and biochemical traits of French bean seeds. However, the French bean has a relatively high cobalt chloride tolerance capacity, so that it showed good tolerance to different concentrations of cobalt chloride for up to 9 days.
Highlights:
1- The effect of different concentrations of cobalt chloride and different types of priming on the germination of French bean seeds was investigated.
2- French bean seedling protein content increased under the influence of hormone priming.
Introduction: The germination stage ensures the durability, establishment, and final yield of plants. The final density of plants per unit area is resulted when the planted seeds germinate fully and with adequate rate. Plants mainly undergo abiotic stresses that are a considerable constraint for agricultural production worldwide. Seed priming is one of the simplest and cheap methods to improve seed germination, acceleration of seedling growth and establishment, uniformity, reduction of flowering time, vigorous seedling production, etc which leads to improved quality and yield of crop under stressful and non-stressful conditions. This study aimed to investigate the effect of different types of priming on enzymatic and physiological changes of French bean seed under cobalt chloride stress conditions.
Materials and Methods: To examine the effect of priming on germination indices, the activity of antioxidant enzymes and proteins in French bean under cobalt chloride stress, a factorial experiment was performed in a completely randomized design with three replications. These factors included four levels: seed priming with 100 mg / l salicylic acid and mannitol, control and distilled water (no priming), and three levels of cobalt chloride stress (0, 100, and 200 mg / l).
Results: The results showed that with increasing cobalt chloride concentration, germination indices (germination rate, root length, root fresh weight, stem fresh weight and stem dry weight) decreased. However, in the pretreatment of distilled water, salicylic acid, and mannitol there was a significant increase in germination indices (mean germination time, radicle length, plumule length, fresh radicle weight, and weight plumule) was observed. The interaction effect of priming and cobalt chloride caused an increase in radicle dry weight compared to control × stress treatment. The highest values of fresh radicle weight (14.4 g), dry weight of plumule (0.27 g), and plumule length (0.17 cm) were obtained in hydro priming pretreatment. The results also showed that hormone priming under stress conditions of 200 and 100 mg / l cobalt chloride increased protein content and polyphenol oxidase activity of French bean seedlings compared with the control treatment, respectively. Osmo priming increased the activity of catalase and peroxidase enzymes under stress and non-stress conditions resulted by 200 mg / l cobalt chloride.
Conclusion: according to the results of the present study, cobalt chloride reduced some physiological and biochemical traits of French bean seeds. However, the French bean has a relatively high cobalt chloride tolerance capacity, so that it showed good tolerance to different concentrations of cobalt chloride for up to 9 days.
Highlights:
1- The effect of different concentrations of cobalt chloride and different types of priming on the germination of French bean seeds was investigated.
2- French bean seedling protein content increased under the influence of hormone priming.
Article number: 7
Type of Study: Research |
Subject:
Seed Physiology
Received: 2022/01/14 | Revised: 2024/02/21 | Accepted: 2022/05/23 | ePublished: 2022/12/11
Received: 2022/01/14 | Revised: 2024/02/21 | Accepted: 2022/05/23 | ePublished: 2022/12/11
References
1. Abd-Alla, M.H., Bagy, M.K., Wahab A. and Bashandy, S.R. 2014. Activation of Rhizobium tibeticum with flavonoids anhances nodulation, nitrogen fixation and growth of fenugreek (Trigonella foenum-graecum L.) grown in cobalt polluted soil. Archives of Environmental Contamination and Toxicology, 66(2): 303-315 [DOI:10.1007/s00244-013-9980-7] [PMID]
2. Abdul-Baki, A.A. and Anderson, J.D. 1973. Vigor determination in soybean seed by multiple criteria. crop science, 13(6): 630-633. [DOI:10.2135/cropsci1973.0011183X001300060013x]
3. Agrawal, R. 2003. Seed technology. Publishing Co. Pvt. Ltd. New Delhi, India, 829p.
4. Amini, F., Balouchi, H., Movahhedi Dehnavi, M. and Attarzadeh, M. 2015. Effects of different concentrations of heavy metals application on germination indices and seed vigor of Pinto bean (Phaseolus vulgaris L.). Iranian Seed Science and Research, 3(2): 95-105. [In Persian with English Summary].
5. Amoghein, M.B., Amoghein, R.S. Tobeh A. and Jamaati-e-Somarin, S. 2013. The effect of osmopriming and hydropriming on the different index of germination & early growth of wheat under salty stress. International Research Journal of Applied Basic Science, 4: 1924-1931.
6. Amooaghaie, R. 2011. The effect of hydro and osmopriming on alfalfa seed germination and antioxidant defenses under salt stress. African Journal of Biotechnology, 10(3): 6269-6275. [DOI:10.5897/AJB11.1984]
7. Aymen, E.M. 2018. Seed Priming with Plant Growth Regulators to Improve Crop Abiotic Stress Tolerance. In: Rakshit, A. and Singh, H.B. (eds.). Advances in Seed Priming. Institute of Agricultural Sciences, BHU, Varanasi, Uttar Pradesh, India, 95-106. [DOI:10.1007/978-981-13-0032-5_6]
8. Balestrasse, K.B., Gardey, L., Gallego S.M. and Tomaro, M.L. 2001. Response of antioxidant defense system in soybean nodules and roots subjected to cadmium stress. Journal of Plant Physiology, 28: 497-504. [DOI:10.1071/PP00158]
9. Bezrukova, M.V., Fatkhutdinova, R.A. and Shakirova, F.M. 2016. Protective effect of wheat germ agglutinin on the course of mitosis in the roots of Triticum aestivum seedlings exposed to cadmium. Russian Journal of Plant Physiology, 63(3): 358-364. [DOI:10.1134/S102144371603002X]
10. Bose, B., Kumar, M. Singhal, R.K. and Mondal, S. 2018. Impact of Seed Priming on the Modulation of Physico-chemical and Molecular Processes During Germination, Growth, and Development of Crops. In: Rakshit, A. and Singh, H.B. (eds.). Advances in Seed Priming. Institute of Agricultural Sciences, BHU, Varanasi, Uttar Pradesh, India, 23-40. [DOI:10.1007/978-981-13-0032-5_2] [PMCID]
11. Bradford, M.M. 1976. A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254. [DOI:10.1016/0003-2697(76)90527-3] [PMID]
12. Chatterjee, C., Gopal, R. and Dube, B.K. 2006. Physiological and biochemical responses of French bean to excess cobalt. Journal of Plant Nutrition, 29: 127-136. [DOI:10.1080/01904160500416513]
13. Chaudhari, B.H., Parmar, J.K., Mali, R.H. and Bumbadiya, N.H. 2017. Effect of Co level and FYM on growth and yield of fodder maize. International Journal of Chemical Studies, 5(1): 327-329. [DOI:10.18782/2320-7051.2631]
14. Eftekhar, N., Fallah, S., Abbasi Sooraki, A., Khodaverdiloo, H. and Rahimi, A. 2019. Effect of salicylic acid and potassium nitrate pretreatment on enhancing the sunflower tolerance in contaminated soils with cadmium. Iranian Journal of Seed Sciences and Research, 6(2): 161-175. [In Persian with English Summary].
15. Eskandari, H. 2012. Seed quality variation of crop plants during seed development and maturation. International Journal of Plant Production, 3(11): 557-560.
16. Ghasemie Pirbaluti, A. and Golparvar, A.R. 2005. A study on morphological and physiological traits of some of the ordinary bean in shahrekord area. In: First National Conference on Pulses. November 20-21, 2005. Ferdowsi University of Mashhad. p88. [In Persian with English Summary].
17. Gul, F., Arfan, M., Shahbaz, M. and Basra, S. 2020. Salicylic acid seed priming modulates morphology, nutrient relations and photosynthetic attributes of wheat grown under cadmium stress. International Journal of Agriculture and Biology, 23: 197-204.
18. Guo, Q., Meng, L., Mao, P.C., Jia Y.Q. and Shi, Y.J. 2013. Role of exogenous salicylic acid in alleviating cadmium-induced toxicity in Kentucky bluegrass. Biochemical Systematics and Ecology, 50: 269-276. [DOI:10.1016/j.bse.2013.05.002]
19. Hayat, S., Ali, B. and Ahmad, A. 2007. Salicylic acid a plant hormone. In: Hayat, S., Ahmad, A. (eds.). Biosynthesis, metabolism and physiological role in plants. Springer Science & Business Media, 1-14. [DOI:10.1007/1-4020-5184-0_1]
20. Horváth, E., Szalai, G. and Janda, T. 2007. Induction of abiotic stress tolerance by salicylic acid signaling. Journal of Plant Growth Regulation, 26(3): 290-300. [DOI:10.1007/s00344-007-9017-4]
21. ISTA., 2009. International rules for seed testing. The International Seed Testing Association. Zurich. Switzerland.
22. Jadoon, S. and Malik, A. 2018. A Review of formation, toxicity of reactive oxygen species by heavy metals and tolerance in plants. International Journal of Biochemistry Research and Review, 21(2): 1-12. [DOI:10.9734/IJBCRR/2018/38670]
23. Jisha, K.C., Vijayakumari, K. and Puthur, J.T. 2013. Seed priming for abiotic stress tolerance: an overview. Acta Physiologiae Plantarum, 35(5): 1381-1396. [DOI:10.1007/s11738-012-1186-5]
24. Karuppanapandian, T. and Kim, W. 2013. Cobalt-induced oxidative stress causes growth inhibition associated with enhanced lipid peroxidation and activates antioxidant responses in Indian mustard (Brassica juncea L.) leaves. Acta Physiologiae Plantarum, 35: 2429-2443. [DOI:10.1007/s11738-013-1277-y]
25. Khazaei, H.R., Nezami, A., Saadatian, B., Pishe, O.A. and Pordel, R. 2016. Effect of seed priming on seedling growth of barley (Hordeum vulgare L.), under salinity stress in phytogel. Environmental Stresses Crop Sciences, 9(1): 87-97. [In Persian with English Summary].
26. Kranner, I. and Colville, L. 2011. Metals and seeds: biochemical and molecular implications and their significance for seed germination. Environmental and Experimental Botany, 72: 93-105. [DOI:10.1016/j.envexpbot.2010.05.005]
27. Liu, Z., Ding, Y., Wang, F., Ye, Y. and Zhu, C. 2016. Role of salicylic acid in resistance to cadmium stress in plants. Plant Cell Reports, 35(4): 719-731. [DOI:10.1007/s00299-015-1925-3] [PMID]
28. Lwalaba, J.L.W., Zvobgo, G., Fu, L., Zhang, X., Mwamba, T.M., Muhammad, N., Mundende, R.P.M. and Zhang, G. 2017a. Alleviating effects of calcium on cobalt toxicity in two barley genotypes differing in cobalt tolerance. Ecotoxicology and Environmental Safety, 139: 488-495. [DOI:10.1016/j.ecoenv.2017.02.019] [PMID]
29. Lwalaba, J.L.W., Zvobgo, G., Mwamba, M., Ahmed, I.M., Mukobo, R.P.M. and Zhang, G. 2017b. Subcellular distribution and chemical forms of Co2+ in three barley genotypes under different Co2+ levels. Acta Physiologiae Plantarum, 39(4): 102. [DOI:10.1007/s11738-017-2400-2]
30. Mahmoodi Tarkhorani, S., Sanjarian Dehaghani, F. and Monsef Shokri, M. 2017. The effect of salicylic acid treatment on the antioxidant enzyme activities in Thymus vulgaris seedlings. Modares Journal of Biotechnology, 10(1): 37-44. [In Persian with English Summary].
31. Marquez Garsia, B., Marquez, C., Sanjose, I., Nieva, F.J.J., Rodriguez Rubio, P. and MunozRodriguez, A.F. 2013. The effects of heavy metals on germination and seedling characteristics in two halophyte species in Mediterranean marshes. Marine Pollution Bulletin, 70: 119-124. [DOI:10.1016/j.marpolbul.2013.02.019] [PMID]
32. Mousavi, S.E., Omidi, H., Mirshekar, Sh. and Bazvand, F. 2019. Effects of priming on germination, growth and physiologic indices in mother line seed of rapeseed (Brassica napus L.) Neptunecultivar under drought stress. Journal of Seed Research, 9(3): 11-21. [In Persian with English Summary].
33. Muthaura, C., Mucheru-Muna, M., Zingore, S., Kihara, J. and Muthamia, J. 2017. Effect of application of different nutrients on growth and yield parameters of maize (Zea mays), case of Kandara Murang'a County. ARPN Journal of Agricultural and Biological Science, 12(1): 19-33.
34. Nouairi, I., Jalali, K., Zribi, F., Barhoumi, F., Zribi, K. and Mhadhbi, H. 2019. Seed priming with calcium chloride improves the photosynthesis performance of faba bean plants subjected to cadmium stress. Photosynthetica, 57(2): 438-445. [DOI:10.32615/ps.2019.055]
35. Serida, K., Mohammad, B.A., Eun, J.H. and Kee, Y.P. 2008. Copper toxicity in Withania somnifera: growth and antioxidant enzymes responses of in vitro grown plants. Environmental and Experimental Botany, 64: 279-285. [DOI:10.1016/j.envexpbot.2008.02.004]
36. Sharma, V., Naugraiya, M.N. and Tomar, G.S. 2018. Toxic effects of cobalt, chromium, lead and nickel chloride on growth performance of siris (Albizia spp.). International Journal of Chemical Studies, 6: 2407-2410.
37. Shulan, Z., Qing, L., Yanting, Q. and Lian, D. 2010. Responses of root growth and protective enzymes to copper stress in turf grass. Acta Biologica Cracoviensia Series Botanica, 52(2): 7-11. [DOI:10.2478/v10182-010-0017-5]
38. Singh, H., Jassal, R.K., Keng, J.S., Sandhu, S.S., Kang H. and Grewal, K. 2015. Seed priming techniques in field crops - a review. Agricultural Review, 36(4): 251-264. [DOI:10.18805/ag.v36i4.6662]
39. Soltani, A., Galeshi, S., Zeinali, E. and Latifi, N. 2001. Genetic variation for and interrelationships among seed vigor traits in wheat from the Caspian Sea voasts of Iran. Seed Science and Technology, 29(3): 653-662.
40. Srivastava, S., Srivastava, A.K., Suprasanna, P. and D'Souza, S.F. 2013a. Identification and profiling of arsenic stress induced microRNA in Brassica juncea. Journal of Experimental Botany, 64(1): 303-315. [DOI:10.1093/jxb/ers333] [PMID]
41. Srivastava, S., Verma, P.C., Chaudhary, V., Singh, N., Abhilash, P.C. and Kumar, K.V. 2013b. Influence of inoculation of arsenic-resistant Staphylococcus arlettae on growth and arsenic uptake in Brassica juncea (L.) Czern. var. R-46. Journal of Hazardous Materials, 262: 1039-1047. [DOI:10.1016/j.jhazmat.2012.08.019] [PMID]
42. Sudhakar, C., Lakshmi, A. and Giridara Kumar, S. 2001. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science, 167: 613-619. [DOI:10.1016/S0168-9452(01)00450-2]
43. Wael, M.S., Mostafa, M.R., Taia, A.A.E.M., Saad, M.H. and Magdi, T.A. 2015. Alleviation of cadmium toxicity in common bean (Phaseolus vulgaris L.) plants by the exogenous application of salicylic acid. The Journal of Horticultural Science and Biotechnology, 90(1): 83-91.
https://doi.org/10.1080/14620316.2015.11513156 [DOI:10.1080/14620316.2015.11513157]
44. Wendling, L.A., Kirby, J.K. and McLaughlin, M.J. 2009. Aging effects on cobalt availability in soils. Environmental Toxicology and Chemistry, 28(8): 1609-1617. [DOI:10.1897/08-544.1] [PMID]
45. Wilkinson, S., Kudoyarova, G.R., Veselov, D.S., Arkhipova, T.N. and Davies, W.J. 2012. Plant hormones interactions: Innovative target for plant breeding and management. Journal of Experimental Botany, 63(9): 3499-3509. [DOI:10.1093/jxb/ers148] [PMID]
46. Wyszkowski, M., Wyszkowska, J. and Radziemska, M. 2009. Macroelement content in field of oats (Avena sativa L.) cultivated on soils contaminated with copper, zinc, tin, cobalt and manganese. Ecological Chemistry and Engineering. A, 16(10): 1387-1394.
47. Zeng, L., Yang, Y., Sun, Y., Du, Z., Xie, Z., Zhou, T. and Kong, W. 2014. Age-Related Decrease in the Mitochondrial Sirtuin Deacetylase Sirt3 Expression Associated with ROS Accumulation in the Auditory Cortex of the Mimetic Aging Rat Model. PLOS one, 9(2): e88019. [DOI:10.1371/journal.pone.0088019] [PMID]
48. Zhang, Y., Xu, S., Yang, S. and Chen, Y. 2015. Salicylic acid alleviates cadmium-induced inhibition of growth and photosynthesis through upregulating antioxidant defense system in two melon cultivars (Cucumis melo L.). Protoplasma, 252(3): 911-924. [DOI:10.1007/s00709-014-0732-y] [PMID]
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